Abstract:
:XIST is an X-linked, non-coding gene responsible for the cis induction of X-chromosome inactivation (XCI). Knockout of the XIST allele on an active X chromosome abolishes erroneous XCI and enhances the in vivo development of cloned mouse embryos by more than 10-fold. This study aimed to investigate whether a similar manipulation would improve cloning efficiency in pigs. A male, porcine kidney cell line containing an EGFP insert in exon 1 of the XIST gene, resulting in a knockout allele (XIST-KO), was generated by homologous recombination using transcription activator-like effector nucleases (TALENs). The expression of X-linked genes in embryos cloned from the XIST-KO kidney cells was significantly higher than in male embryos cloned from wild-type (WT) kidney cells, but remained lower than that of in vivo fertilization-produced counterparts. The XIST-KO cloned embryos also had a significantly lower blastocyst rate and a reduced full-term development rate compared to cloned WT embryos. These data suggested that while mutation of a XIST gene can partially rescue abnormal XCI, it cannot improve the developmental efficiency of cloned male porcine embryos-a deficiency that may be caused by incomplete rescue of abnormal XCI and/or by long-term drug selection of the XIST-KO nuclear donor cells, which might adversely affect the developmental efficiency of embryos created from them.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Yang Y,Wu D,Liu D,Shi J,Zhou R,He X,Quan J,Cai G,Zheng E,Wu Z,Li Zdoi
10.1002/mrd.22808subject
Has Abstractpub_date
2017-06-01 00:00:00pages
525-534issue
6eissn
1040-452Xissn
1098-2795journal_volume
84pub_type
杂志文章abstract::An antiserum, designated R4 and raised against denatured hamster acrosomes, was shown to localize specifically to the acrosomal region of hamster, rat, mouse, and human spermatozoa, and to inhibit both hamster and human sperm-oocyte binding in vitro. Following screening of a human testis lambda gt11 cDNA expression li...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080340308
更新日期:1993-03-01 00:00:00
abstract::Bone morphogenetic proteins (BMPs) belong to the transforming growth factor-beta (TGFβ) superfamily. BMPs mediate a highly conserved signal transduction cascade through the type-I and type-II serine/threonine kinase receptors and intracellular Smad proteins, which regulate multiple developmental and homeostatic proces...
journal_title:Molecular reproduction and development
pub_type: 杂志文章,评审
doi:10.1002/mrd.22199
更新日期:2013-09-01 00:00:00
abstract::The effects of cryopreservation on the frequency and type of chromosomal abnormalities in human sperm were investigated. Employing a technique that enables direct visualization of human sperm chromosomes following in vitro penetration of hamster oocytes, sperm samples from 10 normal men were examined before and after ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080300213
更新日期:1991-10-01 00:00:00
abstract::Eggs of Ascidia ceratodes and Phallusia mammillata block polyspermy by releasing a phosphatidylinositol-linked glycosidase from the follicle cell and egg surface that binds to and blocks all unoccupied sperm binding sites on the vitelline coat. Release of this glycosidase is thought to be under the control of a membra...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199909)54:1<69::AID-MRD10>
更新日期:1999-09-01 00:00:00
abstract::The oviduct is a dynamic organ which modulates gamete physiology. Sperm-oviduct interaction provides the formation of a sperm storage reservoir and allows the selection of sperm with certain qualities in eutherian mammals. In sows, the oviductal sperm binding glycoprotein (SBG) has been proposed to be involved in sper...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20958
更新日期:2009-04-01 00:00:00
abstract::The homeobox-containing gene family plays a pivotal role in regulating, patterning, and axial morphogenesis in the developing embryo. But there is still very little known about the expression and function of these genes in mammalian oocytes and preimplantation stage embryos. In this study we have used degenerate prime...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1091
更新日期:2001-11-01 00:00:00
abstract::The mitochondrial probe 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolyl-carbocyan ine iodide (JC-1) not only identifies mitochondria exhibiting low membrane potentials by the emission of green fluorescence (range, 510-520 nm) but also differentiates these from mitochondria exhibiting relatively high membrane ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199906)53:2<222::AID-MRD11
更新日期:1999-06-01 00:00:00
abstract::The nuclear matrix is thought to be responsible for DNA organization, DNA replication, RNA synthesis, and RNA processing. We have looked for the presence of nuclear matrix antigens during early mouse embryogenesis. Antibodies to peripheral and interior antigens (P1, Pl1, Pl2, and lamin B) were used to immunolocalize n...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080320304
更新日期:1992-07-01 00:00:00
abstract::A porcine interleukin-6 (pIL-6) cDNA has been cloned from pig spleen cDNA library to provide information that would allow us to study IL-6 mRNA expression during pregnancy of several domestic Artiodactyla. The cDNA is 1058 bp long and with a single open reading frame that encodes a 212 amino acid polypeptide with 28-r...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080320404
更新日期:1992-08-01 00:00:00
abstract::Derivation and stable maintenance of porcine induced pluripotent stem cells (piPSCs) is challenging. We herein systematically analyzed two piPSC lines, derived by lentiviral transduction and cultured under either leukemia inhibitory factor (LIF) or fibroblast growth factor (FGF) conditions, to shed more light on the u...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22771
更新日期:2017-03-01 00:00:00
abstract::Previously, we showed that the gonadotropin-induced expansion of bovine cumulus oophorus occurs concomitantly with the rearrangement of microfilaments (MFs) inside cumulus cell cytoplasm (Sutovský et al., 1993: Biol Reprod 49:1277-1287; Sutovský et al., 1994: Reprod Nutr Dev 34:415-425) and that cumulus expansion in c...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080410415
更新日期:1995-08-01 00:00:00
abstract::This study was conducted to investigate the effect of recipient activation time on the chromatin structure and development of bovine nuclear transfer embryos. Serum-starved skin cells were electrofused to enucleated oocytes, activated 1-5 hr after fusion, and cultured in vitro. Some fused eggs were fixed at each time ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20131
更新日期:2004-11-01 00:00:00
abstract::In pigs, the morphology and cell number of in vitro-produced blastocysts are inferior to those of their in vivo counterparts. The objective of this study was to increase developmental competence and to gain an understanding of cell allocation in blastocysts derived from the aggregation of four-cell stage porcine embry...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20728
更新日期:2007-11-01 00:00:00
abstract::Formation and maintenance of testis cords during embryogenesis are essential for establishing testicular structure and function in adults. At least five genes (Wt1, Dhh, Sox8/Sox9, and Dax1) appear to be required for the maintenance of testis cord integrity in mice. Here, we report that RFX1 is specifically expressed ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22660
更新日期:2016-07-01 00:00:00
abstract::Sperm-mediated gene transfer in vertebrates has undergone various developments over the last few years, in different laboratories. In the present study, we microinjected a circular plasmid, carrying the lacZ reporter gene mixed with noncommercial cationic lipids, into the seminiferous tubules of anesthetized adult mic...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.10143
更新日期:2002-08-01 00:00:00
abstract::We report the primary structures of two mRNA species (GH1 and GH2), each predicted from the cloned cDNA and genomic gene sequences, that encode growth hormone in rainbow trout (Salmo gairdneri). Both GH1 and GH2 mRNA contain open reading frames comprising 630 nucleotides and encode 210 amino acid residues, of which 11...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080010104
更新日期:1988-01-01 00:00:00
abstract::Haprin (TRIM36) is a ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. It is expressed in the testes in both mice and humans and is thought to be involved in spermiogenesis, the acrosome reaction, and fertilization. However, the functional role of Haprin i...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.23344
更新日期:2020-05-01 00:00:00
abstract::The expression of both inhibin alpha- and inhibin/activin beta A-subunit mRNA was examined in equine uteroplacental tissues collected during pregnancy (days 90 to 300). Northern blot analysis revealed that 5 transcripts (7.0, 4.1, 3.4, 2.6, 1.5 kb) of beta A-subunit were present, and the most abundantly expressed tran...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199708)47:4<363::AID-MRD2>
更新日期:1997-08-01 00:00:00
abstract::We have previously shown that bovine oocytes parthenogenetically activated after 40 hours (hr) of in vitro maturation proceed through the cell cycle faster than those after 20 hr of maturation. In the present study, we used this model of different speed of nuclear progression to investigate the correlation of two hall...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.10072
更新日期:2002-05-01 00:00:00
abstract::The text below is a transcript of the keynote address Kenneth Manning gave at the symposium honoring E. E. Just that was held on the campus of Howard University on November 21, 2008. In his talk, Manning reflects on his experiences researching and writing Black Apollo of Science: The Life of Ernest Everett Just, his p...
journal_title:Molecular reproduction and development
pub_type: 传,历史文章,杂志文章
doi:10.1002/mrd.21087
更新日期:2009-10-01 00:00:00
abstract::Nuclear import of plasmid DNA mediated by a nuclear localization signal (NLS) derived from SV40 T antigen was investigated in a cell-free extract. In vitro assembled sea urchin male pronuclei were incubated in a 100,000g supernatant of a zebrafish fertilized egg lysate, together with fluorescently labeled plasmid DNA ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199612)45:4<431::AID-MRD4>
更新日期:1996-12-01 00:00:00
abstract::We describe the cloning and characterization of a partial cDNA, x121, that represents an RNA, which is localized in the animal hemisphere of Xenopus oocytes. This RNA is also detected in an animal to vegetal gradient during early cleavage stages. The x121 RNA titer decreases from fertilization through the gastrula sta...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080280405
更新日期:1991-04-01 00:00:00
abstract::Dickkopf-like 1 (DkkL1) is related to the Dickkopf gene family, a group of proteins that are characterized as secreted antagonists of Wingless (Wnt) signal transduction proteins. DkkL1 mRNA is found in preimplantation mouse embryos and in developing neural tissue, but in adults it is found primarily in the testes. In ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20314
更新日期:2005-08-01 00:00:00
abstract::In mammals, the nucleolus of full-grown oocyte is essential for embryonic development but not for oocyte maturation. In our study, the role of the growing oocyte nucleolus in oocyte maturation was examined by nucleolus removal and/or transfer into previously enucleolated, growing (around 100 µm in diameter) or full-gr...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.21320
更新日期:2011-06-01 00:00:00
abstract::So far, standard follicle culture systems can produce blastocyst from less than 40% of the in vitro matured oocytes compared to over 70% in the in vivo counterpart. Because the capacity for embryonic development is strictly associated with the terminal stage of oocyte growth, the nuclear maturity status of the in vitr...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20762
更新日期:2008-01-01 00:00:00
abstract::Expression of ovine interferon-tau (oIFNtau) genes, essential for the maternal recognition of pregnancy in ruminant ungulates, is restricted to the trophoblast and is not detected in any other cell types or tissues. Substantial secretion of oIFNtau starts on day 12-13 of pregnancy (day 0 = day of estrus), reaches the ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20002
更新日期:2004-04-01 00:00:00
abstract::Seminiferous tubules of the testis and epididymal tubules in adult rodents are enveloped by contractile myoid cells, which move sperm and fluids along the male reproductive tract. Myoid cells in the testis influence Sertoli cells by paracrine signaling, but their role in the epididymis is unknown. Electron microscopy ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22649
更新日期:2016-06-01 00:00:00
abstract::The internally fertilizing primitive frog Ascaphus truei (family Ascaphidae) from the Pacific Northwest is the only frog with an intromittent organ. The more advanced neobatrachian frog Eleutherodactylus coqui (family Leptodactylidae) from Puerto Rico has secondarily acquired internal fertilization but mates by cloaca...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20207
更新日期:2005-03-01 00:00:00
abstract::Plac1 is a recently identified, X-linked gene whose expression is restricted primarily to cells of the trophoblast lineage. It localizes to a chromosomal locus previously implicated in placental growth. We therefore sought to determine if Plac1 is necessary for placental and embryonic development by examining a mutant...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22062
更新日期:2012-08-01 00:00:00
abstract::In mouse oocytes, meiotic induction by pharmacological activation of PRKA (adenosine monophosphate-activated protein kinase; formerly known as AMPK) or by hormones depends on stimulation of fatty acid oxidation (FAO). PRKA stimulates FAO by phosphorylating and inactivating acetyl CoA carboxylase (ACAC; formerly ACC), ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22505
更新日期:2015-09-01 00:00:00