Mutation of the XIST gene upregulates expression of X-linked genes but decreases the developmental rates of cloned male porcine embryos.

Abstract:

:XIST is an X-linked, non-coding gene responsible for the cis induction of X-chromosome inactivation (XCI). Knockout of the XIST allele on an active X chromosome abolishes erroneous XCI and enhances the in vivo development of cloned mouse embryos by more than 10-fold. This study aimed to investigate whether a similar manipulation would improve cloning efficiency in pigs. A male, porcine kidney cell line containing an EGFP insert in exon 1 of the XIST gene, resulting in a knockout allele (XIST-KO), was generated by homologous recombination using transcription activator-like effector nucleases (TALENs). The expression of X-linked genes in embryos cloned from the XIST-KO kidney cells was significantly higher than in male embryos cloned from wild-type (WT) kidney cells, but remained lower than that of in vivo fertilization-produced counterparts. The XIST-KO cloned embryos also had a significantly lower blastocyst rate and a reduced full-term development rate compared to cloned WT embryos. These data suggested that while mutation of a XIST gene can partially rescue abnormal XCI, it cannot improve the developmental efficiency of cloned male porcine embryos-a deficiency that may be caused by incomplete rescue of abnormal XCI and/or by long-term drug selection of the XIST-KO nuclear donor cells, which might adversely affect the developmental efficiency of embryos created from them.

journal_name

Mol Reprod Dev

authors

Yang Y,Wu D,Liu D,Shi J,Zhou R,He X,Quan J,Cai G,Zheng E,Wu Z,Li Z

doi

10.1002/mrd.22808

subject

Has Abstract

pub_date

2017-06-01 00:00:00

pages

525-534

issue

6

eissn

1040-452X

issn

1098-2795

journal_volume

84

pub_type

杂志文章
  • Human sperm-egg binding is inhibited by peptides corresponding to core region of an acrosomal serine protease inhibitor.

    abstract::An antiserum, designated R4 and raised against denatured hamster acrosomes, was shown to localize specifically to the acrosomal region of hamster, rat, mouse, and human spermatozoa, and to inhibit both hamster and human sperm-oocyte binding in vitro. Following screening of a human testis lambda gt11 cDNA expression li...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.1080340308

    authors: Moore A,Penfold LM,Johnson JL,Latchman DS,Moore HD

    更新日期:1993-03-01 00:00:00

  • Repulsive guidance molecules (RGMs) and neogenin in bone morphogenetic protein (BMP) signaling.

    abstract::Bone morphogenetic proteins (BMPs) belong to the transforming growth factor-beta (TGFβ) superfamily. BMPs mediate a highly conserved signal transduction cascade through the type-I and type-II serine/threonine kinase receptors and intracellular Smad proteins, which regulate multiple developmental and homeostatic proces...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章,评审

    doi:10.1002/mrd.22199

    authors: Tian C,Liu J

    更新日期:2013-09-01 00:00:00

  • Effect of cryopreservation on the frequency of chromosomal abnormalities and sex ratio in human sperm.

    abstract::The effects of cryopreservation on the frequency and type of chromosomal abnormalities in human sperm were investigated. Employing a technique that enables direct visualization of human sperm chromosomes following in vitro penetration of hamster oocytes, sperm samples from 10 normal men were examined before and after ...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.1080300213

    authors: Martin RH,Chernos JE,Rademaker AW

    更新日期:1991-10-01 00:00:00

  • Activation of follicle cell surface phospholipase by tyrosine kinase dependent pathway is an essential event in Ascidian fertilization.

    abstract::Eggs of Ascidia ceratodes and Phallusia mammillata block polyspermy by releasing a phosphatidylinositol-linked glycosidase from the follicle cell and egg surface that binds to and blocks all unoccupied sperm binding sites on the vitelline coat. Release of this glycosidase is thought to be under the control of a membra...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/(SICI)1098-2795(199909)54:1<69::AID-MRD10>

    authors: Robert LK,Lucio-Gough LM,Goode CA,Mckinney K,Lambert CC

    更新日期:1999-09-01 00:00:00

  • Annexin A2 is involved in pig (Sus scrofa)sperm-oviduct interaction.

    abstract::The oviduct is a dynamic organ which modulates gamete physiology. Sperm-oviduct interaction provides the formation of a sperm storage reservoir and allows the selection of sperm with certain qualities in eutherian mammals. In sows, the oviductal sperm binding glycoprotein (SBG) has been proposed to be involved in sper...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.20958

    authors: Teijeiro JM,Ignotz GG,Marini PE

    更新日期:2009-04-01 00:00:00

  • Expression of homeobox-containing genes in cDNA libraries derived from cattle oocytes and preimplantation stage embryo.

    abstract::The homeobox-containing gene family plays a pivotal role in regulating, patterning, and axial morphogenesis in the developing embryo. But there is still very little known about the expression and function of these genes in mammalian oocytes and preimplantation stage embryos. In this study we have used degenerate prime...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.1091

    authors: Ponsuksili S,Wimmers K,Adjaye J,Schellander K

    更新日期:2001-11-01 00:00:00

  • Organelle-specific probe JC-1 identifies membrane potential differences in the mitochondrial function of bovine sperm.

    abstract::The mitochondrial probe 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolyl-carbocyan ine iodide (JC-1) not only identifies mitochondria exhibiting low membrane potentials by the emission of green fluorescence (range, 510-520 nm) but also differentiates these from mitochondria exhibiting relatively high membrane ...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/(SICI)1098-2795(199906)53:2<222::AID-MRD11

    authors: Garner DL,Thomas CA

    更新日期:1999-06-01 00:00:00

  • Construction of the nuclear matrix at the transition from maternal to zygotic control of development in the mouse: an immunocytochemical study.

    abstract::The nuclear matrix is thought to be responsible for DNA organization, DNA replication, RNA synthesis, and RNA processing. We have looked for the presence of nuclear matrix antigens during early mouse embryogenesis. Antibodies to peripheral and interior antigens (P1, Pl1, Pl2, and lamin B) were used to immunolocalize n...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.1080320304

    authors: Prather RS,Schatten G

    更新日期:1992-07-01 00:00:00

  • Expression of interleukin-6 in porcine, ovine, and bovine preimplantation conceptuses.

    abstract::A porcine interleukin-6 (pIL-6) cDNA has been cloned from pig spleen cDNA library to provide information that would allow us to study IL-6 mRNA expression during pregnancy of several domestic Artiodactyla. The cDNA is 1058 bp long and with a single open reading frame that encodes a 212 amino acid polypeptide with 28-r...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.1080320404

    authors: Mathialagan N,Bixby JA,Roberts RM

    更新日期:1992-08-01 00:00:00

  • Systematic in vitro and in vivo characterization of Leukemia-inhibiting factor- and Fibroblast growth factor-derived porcine induced pluripotent stem cells.

    abstract::Derivation and stable maintenance of porcine induced pluripotent stem cells (piPSCs) is challenging. We herein systematically analyzed two piPSC lines, derived by lentiviral transduction and cultured under either leukemia inhibitory factor (LIF) or fibroblast growth factor (FGF) conditions, to shed more light on the u...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.22771

    authors: Secher JO,Ceylan A,Mazzoni G,Mashayekhi K,Li T,Muenthaisong S,Nielsen TT,Li D,Li S,Petkov S,Cirera S,Luo Y,Thombs L,Kadarmideen HN,Dinnyes A,Bolund L,Roelen BA,Schmidt M,Callesen H,Hyttel P,Freude KK

    更新日期:2017-03-01 00:00:00

  • F-actin is involved in control of bovine cumulus expansion.

    abstract::Previously, we showed that the gonadotropin-induced expansion of bovine cumulus oophorus occurs concomitantly with the rearrangement of microfilaments (MFs) inside cumulus cell cytoplasm (Sutovský et al., 1993: Biol Reprod 49:1277-1287; Sutovský et al., 1994: Reprod Nutr Dev 34:415-425) and that cumulus expansion in c...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.1080410415

    authors: Sutovský P,Fléchon JE,Pavlok A

    更新日期:1995-08-01 00:00:00

  • Effect of activation time on the nuclear remodeling and in vitro development of nuclear transfer embryos derived from bovine somatic cells.

    abstract::This study was conducted to investigate the effect of recipient activation time on the chromatin structure and development of bovine nuclear transfer embryos. Serum-starved skin cells were electrofused to enucleated oocytes, activated 1-5 hr after fusion, and cultured in vitro. Some fused eggs were fixed at each time ...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.20131

    authors: Choi JY,Kim CI,Park CK,Yang BK,Cheong HT

    更新日期:2004-11-01 00:00:00

  • In vitro development and cell allocation of porcine blastocysts derived by aggregation of in vitro fertilized embryos.

    abstract::In pigs, the morphology and cell number of in vitro-produced blastocysts are inferior to those of their in vivo counterparts. The objective of this study was to increase developmental competence and to gain an understanding of cell allocation in blastocysts derived from the aggregation of four-cell stage porcine embry...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.20728

    authors: Lee SG,Park CH,Choi DH,Kim HS,Ka HH,Lee CK

    更新日期:2007-11-01 00:00:00

  • RFX1 maintains testis cord integrity by regulating the expression of Itga6 in male mouse embryos.

    abstract::Formation and maintenance of testis cords during embryogenesis are essential for establishing testicular structure and function in adults. At least five genes (Wt1, Dhh, Sox8/Sox9, and Dax1) appear to be required for the maintenance of testis cord integrity in mice. Here, we report that RFX1 is specifically expressed ...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.22660

    authors: Wang B,Qi T,Chen SQ,Ye L,Huang ZS,Li H

    更新日期:2016-07-01 00:00:00

  • Transient transmission of a transgene in mouse offspring following in vivo transfection of male germ cells.

    abstract::Sperm-mediated gene transfer in vertebrates has undergone various developments over the last few years, in different laboratories. In the present study, we microinjected a circular plasmid, carrying the lacZ reporter gene mixed with noncommercial cationic lipids, into the seminiferous tubules of anesthetized adult mic...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.10143

    authors: Celebi C,Auvray P,Benvegnu T,Plusquellec D,Jégou B,Guillaudeux T

    更新日期:2002-08-01 00:00:00

  • Rainbow trout has two genes for growth hormone.

    abstract::We report the primary structures of two mRNA species (GH1 and GH2), each predicted from the cloned cDNA and genomic gene sequences, that encode growth hormone in rainbow trout (Salmo gairdneri). Both GH1 and GH2 mRNA contain open reading frames comprising 630 nucleotides and encode 210 amino acid residues, of which 11...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.1080010104

    authors: Agellon LB,Davies SL,Lin CM,Chen TT,Powers DA

    更新日期:1988-01-01 00:00:00

  • Haprin-deficient spermatozoa are incapable of in vitro fertilization.

    abstract::Haprin (TRIM36) is a ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. It is expressed in the testes in both mice and humans and is thought to be involved in spermiogenesis, the acrosome reaction, and fertilization. However, the functional role of Haprin i...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.23344

    authors: Aoki Y,Tsujimura A,Kaseda K,Okabe M,Tokuhiro K,Ohta T,O'Bryan MK,Okuda H,Kitamura K,Ogawa Y,Fujiki T,Wada M,Horie S,Nishimune Y,Tanaka H

    更新日期:2020-05-01 00:00:00

  • Equine inhibin/activin beta A-subunit mRNA is expressed in the endometrial gland, but not in the trophoblast, during pregnancy.

    abstract::The expression of both inhibin alpha- and inhibin/activin beta A-subunit mRNA was examined in equine uteroplacental tissues collected during pregnancy (days 90 to 300). Northern blot analysis revealed that 5 transcripts (7.0, 4.1, 3.4, 2.6, 1.5 kb) of beta A-subunit were present, and the most abundantly expressed tran...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/(SICI)1098-2795(199708)47:4<363::AID-MRD2>

    authors: Yamanouchi K,Hirasawa K,Hasegawa T,Ikeda A,Chang KT,Matsuyama S,Nishihara M,Miyazawa K,Sawasaki T,Tojo H,Tachi C,Takahashi M

    更新日期:1997-08-01 00:00:00

  • Association of MPF, MAPK, and nuclear progression dynamics during activation of young and aged bovine oocytes.

    abstract::We have previously shown that bovine oocytes parthenogenetically activated after 40 hours (hr) of in vitro maturation proceed through the cell cycle faster than those after 20 hr of maturation. In the present study, we used this model of different speed of nuclear progression to investigate the correlation of two hall...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.10072

    authors: Tian XC,Lonergan P,Jeong BS,Evans AC,Yang X

    更新日期:2002-05-01 00:00:00

  • Reflections on E. E. Just, Black Apollo of Science, and the experiences of African American scientists.

    abstract::The text below is a transcript of the keynote address Kenneth Manning gave at the symposium honoring E. E. Just that was held on the campus of Howard University on November 21, 2008. In his talk, Manning reflects on his experiences researching and writing Black Apollo of Science: The Life of Ernest Everett Just, his p...

    journal_title:Molecular reproduction and development

    pub_type: 传,历史文章,杂志文章

    doi:10.1002/mrd.21087

    authors: Manning KR

    更新日期:2009-10-01 00:00:00

  • Nuclear localization signal of SV40 T antigen directs import of plasmid DNA into sea urchin male pronuclei in vitro.

    abstract::Nuclear import of plasmid DNA mediated by a nuclear localization signal (NLS) derived from SV40 T antigen was investigated in a cell-free extract. In vitro assembled sea urchin male pronuclei were incubated in a 100,000g supernatant of a zebrafish fertilized egg lysate, together with fluorescently labeled plasmid DNA ...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/(SICI)1098-2795(199612)45:4<431::AID-MRD4>

    authors: Collas P,Aleström P

    更新日期:1996-12-01 00:00:00

  • x121: a localized maternal transcript in Xenopus laevis.

    abstract::We describe the cloning and characterization of a partial cDNA, x121, that represents an RNA, which is localized in the animal hemisphere of Xenopus oocytes. This RNA is also detected in an animal to vegetal gradient during early cleavage stages. The x121 RNA titer decreases from fertilization through the gastrula sta...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.1080280405

    authors: Kloc M,Reddy BA,Miller M,Eastman E,Etkin LD

    更新日期:1991-04-01 00:00:00

  • DkkL1 (Soggy), a Dickkopf family member, localizes to the acrosome during mammalian spermatogenesis.

    abstract::Dickkopf-like 1 (DkkL1) is related to the Dickkopf gene family, a group of proteins that are characterized as secreted antagonists of Wingless (Wnt) signal transduction proteins. DkkL1 mRNA is found in preimplantation mouse embryos and in developing neural tissue, but in adults it is found primarily in the testes. In ...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.20314

    authors: Kohn MJ,Kaneko KJ,DePamphilis ML

    更新日期:2005-08-01 00:00:00

  • Nucleoli from growing oocytes inhibit the maturation of enucleolated, full-grown oocytes in the pig.

    abstract::In mammals, the nucleolus of full-grown oocyte is essential for embryonic development but not for oocyte maturation. In our study, the role of the growing oocyte nucleolus in oocyte maturation was examined by nucleolus removal and/or transfer into previously enucleolated, growing (around 100 µm in diameter) or full-gr...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.21320

    authors: Kyogoku H,Ogushi S,Miyano T,Fulka J Jr

    更新日期:2011-06-01 00:00:00

  • Effects of gonadotrophins, growth hormone, and activin A on enzymatically isolated follicle growth, oocyte chromatin organization, and steroid secretion.

    abstract::So far, standard follicle culture systems can produce blastocyst from less than 40% of the in vitro matured oocytes compared to over 70% in the in vivo counterpart. Because the capacity for embryonic development is strictly associated with the terminal stage of oocyte growth, the nuclear maturity status of the in vitr...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.20762

    authors: Ola SI,Ai JS,Liu JH,Wang Q,Wang ZB,Chen DY,Sun QY

    更新日期:2008-01-01 00:00:00

  • Increase in DNA methylation downregulates conceptus interferon-tau gene expression.

    abstract::Expression of ovine interferon-tau (oIFNtau) genes, essential for the maternal recognition of pregnancy in ruminant ungulates, is restricted to the trophoblast and is not detected in any other cell types or tissues. Substantial secretion of oIFNtau starts on day 12-13 of pregnancy (day 0 = day of estrus), reaches the ...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.20002

    authors: Nojima H,Nagaoka K,Christenson RK,Shiota K,Imakawa K

    更新日期:2004-04-01 00:00:00

  • Implications of caveolae in testicular and epididymal myoid cells to sperm motility.

    abstract::Seminiferous tubules of the testis and epididymal tubules in adult rodents are enveloped by contractile myoid cells, which move sperm and fluids along the male reproductive tract. Myoid cells in the testis influence Sertoli cells by paracrine signaling, but their role in the epididymis is unknown. Electron microscopy ...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.22649

    authors: Oliveira RL,Parent A,Cyr DG,Gregory M,Mandato CA,Smith CE,Hermo L

    更新日期:2016-06-01 00:00:00

  • Protamines in the internally fertilizing neobatrachian frog Eleutherodactylus coqui.

    abstract::The internally fertilizing primitive frog Ascaphus truei (family Ascaphidae) from the Pacific Northwest is the only frog with an intromittent organ. The more advanced neobatrachian frog Eleutherodactylus coqui (family Leptodactylidae) from Puerto Rico has secondarily acquired internal fertilization but mates by cloaca...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.20207

    authors: Kasinsky HE,Frehlick LJ,Su HW,Ausio J

    更新日期:2005-03-01 00:00:00

  • Plac1 (placenta-specific 1) is essential for normal placental and embryonic development.

    abstract::Plac1 is a recently identified, X-linked gene whose expression is restricted primarily to cells of the trophoblast lineage. It localizes to a chromosomal locus previously implicated in placental growth. We therefore sought to determine if Plac1 is necessary for placental and embryonic development by examining a mutant...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.22062

    authors: Jackman SM,Kong X,Fant ME

    更新日期:2012-08-01 00:00:00

  • Acetyl CoA carboxylase inactivation and meiotic maturation in mouse oocytes.

    abstract::In mouse oocytes, meiotic induction by pharmacological activation of PRKA (adenosine monophosphate-activated protein kinase; formerly known as AMPK) or by hormones depends on stimulation of fatty acid oxidation (FAO). PRKA stimulates FAO by phosphorylating and inactivating acetyl CoA carboxylase (ACAC; formerly ACC), ...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.22505

    authors: Valsangkar DS,Downs SM

    更新日期:2015-09-01 00:00:00