Abstract:
:Oocyte maturation is accompanied by differentiation of surrounding cumulus cells. These cells produce hyaluronic acid (HA) and its storage in intercellular spaces results in expansion of the cells. The cumulus cells also accumulate cyclooxygenase-2 (cox-2) during maturation. Both expansion and cox-2 storage are regulated by FSH and EGF. The aim of this study was to determine whether oocyte meiotic resumption is involved in the regulation of cumulus differentiation or not. We investigated the effects of roscovitine, a reversible inhibitor of meiosis resumption of cattle oocytes on EGF induced expansion and cox-2 expression at the transcript and protein levels respectively (RT-PCR and Western blot), in cumulus oocyte complexes (COCs) and cumulus complexes alone (CCs). EGF induced expansion and cox-2 expression in both COCs and CCs. These effects were prevented by roscovitine, whether in the presence or in the absence of oocyte. However, the oocyte was essential for the reversibility of inhibition by roscovitine. In conclusion, our results indicate that i) oocyte secreted-factors are not essential for cumulus expansion, and ii) roscovitine mediated inhibition of meiotic resumption also respects the functionality of the surrounding somatic cells.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Vigneron C,Nuttinck F,Perreau C,Reinaud P,Charpigny G,Mermillod Pdoi
10.1002/mrd.10265keywords:
subject
Has Abstractpub_date
2003-05-01 00:00:00pages
114-21issue
1eissn
1040-452Xissn
1098-2795journal_volume
65pub_type
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pub_type: 杂志文章
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更新日期:1996-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2017-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2007-02-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2009-09-01 00:00:00
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pub_type: 杂志文章
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