Identification and function of proteolysis regulators in seminal fluid.

abstract：:To investigate the decrease of transcription and to determine the localization of RNA molecules during spermiogenesis in the fern Scolopendrium, we have used the enzyme-gold electron microscope method. During cell differentiation, the labeling decreases over the cytoplasm and increases over the nucleus. In the latter,...

journal_title：Molecular reproduction and development

authors： Rejon E,Bajon C,Blaize A,Robert D

更新日期：1988-01-01 00:00:00

abstract：:Although placental transfer of maternal calcium (Ca(2+)) is a crucial process for fetal development, the biochemical mechanisms are not completely elucidated. Especially, mechanisms of syncytiotrophoblast Ca(2+) extrusion into fetal circulation remain to be established. In the current study we have investigated the ch...

journal_title：Molecular reproduction and development

authors： Moreau R,Daoud G,Masse A,Simoneau L,Lafond J

更新日期：2003-07-01 00:00:00

abstract：:Diandric and digynic triploid mouse embryos were isolated in the morning on day 10 of gestation. The embryos were separated from their extraembryonic membranes, and the latter were analysed cytogenetically by G-banding to establish the ploidy and sex chromosome constitution of these embryos. The diandric triploid embr...

journal_title：Molecular reproduction and development

authors： Kaufman MH,Lee KK,Speirs S

更新日期：1990-04-01 00:00:00

abstract：:Cumulus oocyte complexes (COCs) and cumulus oocyte complexes connected to a piece of the membrane granulosa (COCGs) were isolated from bovine antral follicles with a diameter of 2 to 8 mm. After culture of COCGs without gonadotrophic hormones for 22 hr approximately 50% of the oocytes were still in the germinal vesicl...

journal_title：Molecular reproduction and development

authors： van Tol HT,van Eijk MJ,Mummery CL,van den Hurk R,Bevers MM

更新日期：1996-10-01 00:00:00

abstract：:We have previously reported metabolic cooperation between Sertoli and peritubular myoid cells in terms of synthesis of one of the main testicular extracellular matrix (ECM) constituents, glycosaminoglycans (GAG). This study concerns Sertoli cell ECM-peritubular myoid cell interactions in terms of GAG synthesis. We hav...

journal_title：Molecular reproduction and development

authors： Raychoudhury SS,Blackshaw AW,Irving MG

更新日期：1993-06-01 00:00:00

abstract：:Hyperactivated motility was studied in guinea pig spermatozoa. In the presence of the local anesthetic procaine, a high number of sperm cells (64%) showed hyperactivation when incubated in minimal culture medium with pyruvate, lactate, and glucose. Hyperactivated motility was dependent on glucose in the medium. Sperm ...

journal_title：Molecular reproduction and development

authors： Mújica A,Neri-Bazan L,Tash JS,Uribe S

更新日期：1994-07-01 00:00:00

abstract：:The mRNA translational control protein, Musashi, plays a critical role in cell fate determination through sequence-specific interactions with select target mRNAs. In proliferating stem cells, Musashi exerts repression of target mRNAs to promote cell cycle progression. During stem cell differentiation, Musashi target m...

journal_title：Molecular reproduction and development

authors： Arumugam K,Macnicol MC,Macnicol AM

更新日期：2012-08-01 00:00:00

abstract：:EPPIN (epididymal protease inhibitor; SPINLW1), an antimicrobial cysteine-rich protein containing both Kunitz and whey acidic protein (WAP)-type four disulfide core protease inhibitor consensus sequences, is a target for male contraception because of its critical role in sperm motility. Here, we characterized EPPIN's ...

journal_title：Molecular reproduction and development

authors： Silva EJ,Patrão MT,Tsuruta JK,O'Rand MG,Avellar MC

更新日期：2012-12-01 00:00:00

abstract：:There are five methyl binding domain (MBD) proteins characterized by a methyl CpG-binding domain. Four MBD proteins (MeCP2 and MBDs 1-3) are linked to transcriptional repression and one (MBD4), to DNA repair. During preimplantation development, the embryo undergoes global demethylation following fertilization and sele...

journal_title：Molecular reproduction and development

authors： Ruddock-D'Cruz NT,Xue J,Wilson KJ,Heffernan C,Prashadkumar S,Cooney MA,Sanchez-Partida LG,French AJ,Holland MK

更新日期：2008-01-01 00:00:00

abstract：:RNA interference (RNAi) is an effective tool for studying gene function in oocytes, but no studies have targeted somatic cells of primary cultured cumulus cell-oocyte complexes (COCs). This is probably due to difficulty in introducing RNAi-inducing molecules, such as a short-hairpin RNA (shRNA) gene, into COCs by comm...

journal_title：Molecular reproduction and development

authors： Sugiura K,Su YQ,Eppig JJ

更新日期：2009-06-01 00:00:00

abstract：:Changes in spermatozoan ultrastructure have been studied during spermiogenesis of the slug Arion rufus (Gastropoda, Pulmonata, Stylommatophora). The ovotestis was investigated during the male stage, definite by the presence of spermatozoa. Some peculiar characteristics are shown by early spermatids: Around the nucleus...

journal_title：Molecular reproduction and development

authors： Pastisson C,Lacorre I

更新日期：1996-04-01 00:00:00

abstract：:DNA methylation/demethylation of donor genomes in recipient ooplasm after nuclear transfer occurs in a species-specific way. In cloned rabbit and bovine embryos, repetitive sequences maintain the donor-type methylation status, but typical demethylation of repetitive sequences takes place in cloned porcine embryos. To ...

journal_title：Molecular reproduction and development

authors： Chen T,Zhang YL,Jiang Y,Liu JH,Schatten H,Chen DY,Sun QY

更新日期：2006-03-01 00:00:00

abstract：:Communication between the oocyte and its somatic cells has been shown to be important in oocyte development. Here we examined how the oocyte may be involved in bovine cumulus cell expansion. Intact bovine cumulus oocyte complexes (COC) were obtained by puncturing antral follicles. From the intact COC, oocytectomised c...

journal_title：Molecular reproduction and development

authors： Ralph JH,Telfer EE,Wilmut I

更新日期：1995-10-01 00:00:00

abstract：:We determined the incidence of activation, male pronuclear formation, and apposition of pronuclei in porcine oocytes following intracytoplasmic injection of various porcine sperm components and foreign species spermatozoa, such as that of cattle, mouse or human. The porcine oocytes were activated by injection of a spe...

journal_title：Molecular reproduction and development

authors： Kim NH,Jun SH,Do JT,Uhm SJ,Lee HT,Chung KS

更新日期：1999-05-01 00:00:00

abstract：:Epigenetics includes the study of external factors that can influence the expression of genes by altering the accessibility of DNA through methylation. To investigate the epigenetic influence of season, sperm head shape, and semen storage on placental and fetal tissues, pregnancies were generated in the summer or wint...

journal_title：Molecular reproduction and development

authors： Rempel LA,Krautkramer MM,Parrish JJ,Miles JR

更新日期：2019-04-01 00:00:00

abstract：:Members of the Pumilio (Pum) family of RNA-binding proteins act as translational repressors and are required for germ cell development and asymmetric division. We identified the chicken Pum1 and Pum2 genes and analyzed their expression patterns in various tissues. Comparative sequence analysis of the Pum1 and Pum2 pro...

journal_title：Molecular reproduction and development

authors： Lee JY,Lim JM,Kim DK,Zheng YH,Moon S,Han BK,Song KD,Kim H,Han JY

更新日期：2008-01-01 00:00:00

abstract：:The purposes of this study were to establish and to explore the biological basis of the chronic stress-induced premature ovarian failure (POF) model and to explore the therapeutic effects of the traditional Chinese medicine Muniziqi. Sexually matured female Sprague-Dawley rats were fed with spinach and cilantro in col...

journal_title：Molecular reproduction and development

authors： Keremu A,Yaoliwasi A,Tuerhong M,Kadeer N,Heyi,Yiming A,Yilike X

更新日期：2019-02-01 00:00:00

abstract：:Mouse zygotes or halves of zygotes, containing either a female or a male pronucleus, were fused with ovulated metaphase II oocytes. In 59.7% of the resulting hybrid cells, the pronuclei underwent premature chromosome condensation (PCC). In some of these heterokaryons the 2 pronuclei differed in the dynamics of condens...

journal_title：Molecular reproduction and development

authors： Ciemerych MA,Czołowska R

更新日期：1993-01-01 00:00:00

abstract：:Inhibin α (Inha) gene expression is regulated, in rat granulosa cells, via a cyclic 3',5'-adenosine monophosphate (AMP)-response element (CRE) found in a region of the promoter that is homologous to the human INHA promoter. We previously found that during in vitro cytotrophoblast differentiation, human INHA gene expre...

journal_title：Molecular reproduction and development

authors： Depoix CL,Debiève F,Hubinont C

更新日期：2014-11-01 00:00:00

abstract：:The present study was conducted to investigate the mechanisms underlying fluid movement across the trophectoderm during blastocyst formation by determining whether aquaporins (AQPs) are expressed during early mammalian development. AQPs belong to a family of major intrinsic membrane proteins and function as molecular ...

journal_title：Molecular reproduction and development

authors： Offenberg H,Barcroft LC,Caveney A,Viuff D,Thomsen PD,Watson AJ

更新日期：2000-12-01 00:00:00

abstract：:The presence and possible role of products of nuclear (c-fos and c-jun) and c-ras proto-oncogenes were investigated in preimplantation embryonic development in mice. Polyclonal antibodies to c-fos or c-jun proto-oncogene products did not affect development of in vitro-cultured embryos from two-cell to morula or from m...

journal_title：Molecular reproduction and development

authors： Ahmad K,Naz RK

更新日期：1993-11-01 00:00:00

abstract：:In vitro studies have suggested that the Cables1 gene regulates epithelial cell proliferation, whereas other studies suggest a role in promoting neural differentiation. In efforts to clarify the functions of Cables1 in vivo, we conducted gain- and loss-of-function studies targeting its ortholog (cables1) in the zebraf...

journal_title：Molecular reproduction and development

authors： Groeneweg JW,White YA,Kokel D,Peterson RT,Zukerberg LR,Berin I,Rueda BR,Wood AW

更新日期：2011-01-01 00:00:00

abstract：:Sperm-mediated gene transfer in vertebrates has undergone various developments over the last few years, in different laboratories. In the present study, we microinjected a circular plasmid, carrying the lacZ reporter gene mixed with noncommercial cationic lipids, into the seminiferous tubules of anesthetized adult mic...

journal_title：Molecular reproduction and development

authors： Celebi C,Auvray P,Benvegnu T,Plusquellec D,Jégou B,Guillaudeux T

更新日期：2002-08-01 00:00:00

abstract：:Connexin 43 (Cx43), a gap junction protein expressed in differentiated granulosa cells, is necessary for normal follicular development. Cx43 expression and regulation by epidermal growth factor (EGF) were characterized in immature rabbit granulosa cells. Cx43 mRNA was expressed in the granulosa cells of primary follic...

journal_title：Molecular reproduction and development

authors： Kennedy KL,Floyd AA,Clarkson AM,Lee VH

更新日期：2003-01-01 00:00:00

abstract：:Estrogen receptor mRNA expression was detected in the head and tail parts of the human epididymis by means of reverse transcription polymerase chain reaction (RT-PCR). Immunocytochemical labelling showed that ciliate and nonciliate cells from the epithelium of the efferent ductules possessed strong to moderate nuclear...

journal_title：Molecular reproduction and development

authors： Ergün S,Ungefroren H,Holstein AF,Davidoff MS

更新日期：1997-08-01 00:00:00

abstract：:Lipopolysaccharide (LPS) induces germ cell apoptosis, but its mechanism of action is not clear. One possibility is that LPS regulates the expression of FAS ligand (FASLG) in Sertoli cells, which will then influence germ cell apoptosis. In this study, LPS reduced the viability of cultured, immature boar Sertoli cells i...

journal_title：Molecular reproduction and development

authors： Wang Y,Zhang JJ,Yang WR,Luo HY,Zhang JH,Wang XZ

更新日期：2015-11-01 00:00:00

abstract：:Spindle positioning is an imperative cellular process that regulates a number of different developmental events throughout embryogenesis. The spindle must be properly positioned in embryos not only for the segregation of chromosomes, but also to segregate developmental determinants into different daughter blastomeres....

journal_title：Molecular reproduction and development

authors： Moorhouse KS,Burgess DR

更新日期：2014-10-01 00:00:00

abstract：:Rat epididymal glycoprotein DE associates with the dorsal region of the sperm head during sperm maturation, migrates to the equatorial segment (ES) with the acrosome reaction (AR), and is involved in gamete membrane fusion. In the present study we examined the association of DE with the sperm surface and the relations...

journal_title：Molecular reproduction and development

authors： Cohen DJ,Rochwerger L,Ellerman DA,Morgenfeld MM,Busso D,Cuasnicú PS

更新日期：2000-06-01 00:00:00

abstract：:As a means of determining whether ovarian follicular fluid reaches the functional sperm reservoir in the caudal isthmus of the Fallopian tube shortly after ovulation, 0.01-0.02 ml aliquots of whole or steroid-free follicular fluid were introduced into the distal extremity of the isthmus within 1 hr before ovulation. E...

journal_title：Molecular reproduction and development

authors： Hunter RH,Petersen HH,Greve T

更新日期：1999-11-01 00:00:00

abstract：:The possibility to isolate canine mesenchymal stem cells (MSCs) from foetal adnexa is interesting since several canine genetic disorders are reported to resemble similar dysfunctions in humans. In this study, we successfully isolated, cytogenetically and molecularly characterized, and followed the differentiation pote...

journal_title：Molecular reproduction and development

authors： Filioli Uranio M,Valentini L,Lange-Consiglio A,Caira M,Guaricci AC,L'Abbate A,Catacchio CR,Ventura M,Cremonesi F,Dell'Aquila ME

更新日期：2011-05-01 00:00:00