Research on the establishment of chronic stress-induced premature ovarian failure the rat model and effects of Chinese medicine Muniziqi treatment.

abstract：:The present study aims at quantification of gonadotropin releasing hormone (GnRH) by radioimmunoassay, relative expression of its mRNA by real-time PCR accompanied by its cellular localization in the rat ovary by immunonohistochemistry (IHC) during different time points of pregnancy. To determine the involvement of en...

journal_title：Molecular reproduction and development

authors： Sengupta A,Chakrabarti N,Sridaran R

更新日期：2008-06-01 00:00:00

abstract：:Post-acrosomal WW-domain binding protein (PAWP) is a signaling molecule located in the post-acrosomal sheath (PAS) of mammalian spermatozoa. We hypothesized that the proper integration of PAWP in the sperm PAS is reflective of bull-sperm quality and fertility. Cryopreserved semen samples from 298 sires of acceptable, ...

journal_title：Molecular reproduction and development

authors： Kennedy CE,Krieger KB,Sutovsky M,Xu W,Vargovič P,Didion BA,Ellersieck MR,Hennessy ME,Verstegen J,Oko R,Sutovsky P

更新日期：2014-05-01 00:00:00

abstract：:Connexin 43 (Cx43), a gap junction protein expressed in differentiated granulosa cells, is necessary for normal follicular development. Cx43 expression and regulation by epidermal growth factor (EGF) were characterized in immature rabbit granulosa cells. Cx43 mRNA was expressed in the granulosa cells of primary follic...

journal_title：Molecular reproduction and development

authors： Kennedy KL,Floyd AA,Clarkson AM,Lee VH

更新日期：2003-01-01 00:00:00

abstract：:Enzyme activities were determined quantitatively in individual rat oocytes to study their energy metabolism during maturation. Low hexokinase activity and high activities of lactate dehydrogenase and enzymes in the phosphate pathway, i.e., glucose 6-P and 6-P gluconate dehydrogenases, were characteristic of immature o...

journal_title：Molecular reproduction and development

authors： Tsutsumi O,Satoh K,Taketani Y,Kato T

更新日期：1992-11-01 00:00:00

abstract：:In mouse oocytes, meiotic induction by pharmacological activation of PRKA (adenosine monophosphate-activated protein kinase; formerly known as AMPK) or by hormones depends on stimulation of fatty acid oxidation (FAO). PRKA stimulates FAO by phosphorylating and inactivating acetyl CoA carboxylase (ACAC; formerly ACC), ...

journal_title：Molecular reproduction and development

authors： Valsangkar DS,Downs SM

更新日期：2015-09-01 00:00:00

abstract：:The 26S proteasome is a self-compartmentalizing protease responsible for the degradation of intracellular proteins. This giant intracellular protease is formed by several subunits arranged into two 19S polar caps-where protein recognition and ATP-dependent unfolding occur-flanking a 20S central barrel-shaped structure...

journal_title：Molecular reproduction and development

authors： Kierszenbaum AL

更新日期：2000-10-01 00:00:00

abstract：:Under the influence of progesterone, the porcine uterus synthesizes plasmin/trypsin inhibitor (PTI), a low molecular weight protein (M(r) approximately 14,000) belonging to the Kunitz family of proteinase inhibitors. Here it is demonstrated that mRNA for the same protein is produced by the developing trophoblast durin...

journal_title：Molecular reproduction and development

authors： Duffy JY,Stallings-Mann ML,Trout WE,Roberts RM

更新日期：1997-04-01 00:00:00

abstract：:Zona pellucida thickness was measured daily in zygotes and cleavage stage embryos. Measurements were performed on a Nikon inverted microscope equipped with Hoffman modulation optics, using an ocular micrometer. Zona thickness of each zygote/embryo was measured four times, the zygote/embryo was then "rolled over," and ...

journal_title：Molecular reproduction and development

authors： Garside WT,Loret de Mola JR,Bucci JA,Tureck RW,Heyner S

更新日期：1997-05-01 00:00:00

abstract：:The aim of this study was to evaluate the role of adiponectin in the delayed embryonic development of Cynopterus sphinx. Adiponectin receptor (ADIPOR1) abundance was first observed to be lower during the delayed versus non-delayed periods of utero-embryonic unit development. The effects of adiponectin treatment on emb...

journal_title：Molecular reproduction and development

authors： Anuradha,Krishna A

更新日期：2014-12-01 00:00:00

abstract：:This study investigated the mechanisms for the regulation of intracellular pH in bovine oocytes and embryos. Na(+)/H(+) antiporter activity for the regulation of intracellular pH in the acid to neutral range was detected in both in vitro matured bovine oocytes and in vitro produced embryos. However, the activity of th...

journal_title：Molecular reproduction and development

authors： Lane M,Bavister BD

更新日期：1999-12-01 00:00:00

abstract：:Successful fertilization in mammals depends on the sperm's ability to initiate intracellular Ca2+ oscillations in the egg, a phenomenon that is elicited by Sperm-oocyte activating factors (SOAFs), whose quantitative and/or qualitative defect might result in fertilization failure. One such proposed factor is Post-acros...

journal_title：Molecular reproduction and development

authors： Freour T,Barragan M,Torra-Massana M,Ferrer-Vaquer A,Vassena R

更新日期：2018-02-01 00:00:00

abstract：:In fish, growth hormone (GH), prolactin (PRL) and somatolactin (SL) are three major peptide hormones produced in the pituitary gland. Using reverse transcription-polymerase chain reaction (RT-PCR) and Southern blot analysis of the amplified products, the expression of GH, PRL, and SL genes were determined during the e...

journal_title：Molecular reproduction and development

authors： Yang BY,Greene M,Chen TT

更新日期：1999-06-01 00:00:00

abstract：:Dead-end1 (Dnd1) expression is restricted to the vertebrate germline where it is believed to activate translation of messenger RNAs (mRNAs) required to protect and promote that unique lineage. Nanos1 is one such germline mRNA whose translation is blocked by a secondary mRNA structure within the open reading frame (ORF...

journal_title：Molecular reproduction and development

authors： Aguero T,Jin Z,Owens D,Malhotra A,Newman K,Yang J,King ML

更新日期：2018-12-01 00:00:00

abstract：:An in vitro superfusion method was used to test sex hormone release from different kinds of ovarian follicle (growing follicles, postovulatory follicles, and atretic follicles) in the lizard Podarcis sicula sicula. Sex hormone output changes with the stage of follicle evolution and sexual cycle. Previtellogenetic foll...

journal_title：Molecular reproduction and development

authors： Ciarcia G,Paolucci M,Di Fiore MM

更新日期：1993-07-01 00:00:00

abstract：:Mitochondrial metabolic capacity and DNA replication have both been shown to affect oocyte quality, but it is unclear which one is more critical. In this study, immature oocytes were treated with FCCP or ddC to independently inhibit the respective mitochondrial metabolic capacity or DNA replication of oocytes during i...

journal_title：Molecular reproduction and development

authors： Ge H,Tollner TL,Hu Z,Dai M,Li X,Guan H,Shan D,Zhang X,Lv J,Huang C,Dong Q

更新日期：2012-06-01 00:00:00

abstract：:cAMP response element binding protein (CREB)-binding protein (CBP) and p300 are two structurally related transcriptional co-activators that activate expression of many eukaryotic genes. Current dogma would suggest that these transcriptional co-activators have similar mechanisms of transcription regulation. Studies of ...

journal_title：Molecular reproduction and development

authors： Kwok RP,Liu XT,Smith GD

更新日期：2006-07-01 00:00:00

abstract：:Cumulus oocyte complexes (COCs) and cumulus oocyte complexes connected to a piece of the membrane granulosa (COCGs) were isolated from bovine antral follicles with a diameter of 2 to 8 mm. After culture of COCGs without gonadotrophic hormones for 22 hr approximately 50% of the oocytes were still in the germinal vesicl...

journal_title：Molecular reproduction and development

authors： van Tol HT,van Eijk MJ,Mummery CL,van den Hurk R,Bevers MM

更新日期：1996-10-01 00:00:00

abstract：:In the present study we analyse the nature and the functional significance of the spherical and fibrillo-granular structures appearing in the oocyte nucleus of the lizard Podarcis sicula, following the disappearance of the typical nucleolus. By LM and TEM approaches, we demonstrate that the fibrillo-granuli, containin...

journal_title：Molecular reproduction and development

authors： Tammaro S,Andreuccetti P,Filosa S,Indolfi P,Prisco M,Motta CM

更新日期：1998-12-01 00:00:00

abstract：:Triosephosphate isomerase 1 (TPI1) is a member of the glycolytic pathway, which is a critical source of energy for motility in mouse sperm. By immunoblotting, we detected two male, germ line-specific TPI1 bands (Mr 33,400 and 30,800) as well as the somatic-type band (Mr 27,700). Although all three bands were observed ...

journal_title：Molecular reproduction and development

authors： Ijiri TW,Vadnais ML,Lin AM,Huang AP,Cao W,Merdiushev T,Gerton GL

更新日期：2013-10-01 00:00:00

abstract：:The mRNAs of the nuclear encoded genes, ornithine decarboxylase (ODCase) and poly(ADP)ribose polymerase (PADPRP), and the mitochondrial encoded genes, cytochrome oxidase I and II (COI and COII) and ATPase 6, are differentially expressed during spermatogenesis (Alcivar et al., 1989: Biol Reprod 41:1133; 1989: Dev Biol ...

journal_title：Molecular reproduction and development

authors： Alcivar-Warren A,Trasler JM,Awoniyi CA,Zirkin BR,Hecht NB

更新日期：1996-03-01 00:00:00

abstract：:Full-length cDNA of a novel mouse gene upregulated in late stages of spermatogenic cells was cloned from mouse testis using overlapping RT-PCR and RACE. The mRNA of the gene was expressed mainly in diplotene/pachytene spermatocytes, round and elongating spermatids. We named this gene as SRG-L (Spermatogenesis Related ...

journal_title：Molecular reproduction and development

authors： Ma Q,Wang H,Guo R,Wang H,Ge Y,Ma J,Xue S,Han D

更新日期：2006-09-01 00:00:00

abstract：:The presence of two ras-related proteins (22 and 23 kDa) was demonstrated in Xenopus embryonic extracts by selective immunoprecipitation using anti-ras monoclonal antibodies 142-24E05 and Y13-259. We further describe the cytological effects of the microinjection of anti-ras monoclonal antibody Y13-259 into early cleav...

journal_title：Molecular reproduction and development

authors： Miron MJ,Lanoix J,Paiement J

更新日期：1990-04-01 00:00:00

abstract：:Insulators are located at the boundaries of differentially regulated genes and delimit their interactions by establishing independent chromatin structures. Recently, an insulator sequence has been found in the 5'-flanking region of arylsulfatase (ARS) gene from sea urchin. To investigate functional conservation of thi...

journal_title：Molecular reproduction and development

authors： Takada T,Iida K,Akasaka K,Yasue H,Torii R,Tsujimoto G,Taira M,Kimura H

更新日期：2000-11-01 00:00:00

abstract：:In studies of somatic cell nuclear transfer (SCNT), the ability of factors within the oocyte to epigenetically reprogram transferred nuclei is essential for embryonic development of the clone to proceed. However, irregular patterns of X-chromosome inactivation, abnormal expression of imprinted genes, and genomic DNA h...

journal_title：Molecular reproduction and development

authors： Golding MC,Williamson GL,Stroud TK,Westhusin ME,Long CR

更新日期：2011-05-01 00:00:00

abstract：:Fertile offspring from somatic cell nuclear transfer (SCNT) is the goal of most cloning laboratories. For this process to be successful, a number of events must occur correctly. First the donor nucleus must be in a state that is amenable to remodeling and subsequent genomic reprogramming. The nucleus must be introduce...

journal_title：Molecular reproduction and development

authors： Whitworth KM,Prather RS

更新日期：2010-12-01 00:00:00

abstract：:A quantitative primer extension method was used to measure the mass of histone gene transcripts in mature oocytes of the amphibian Xenopus laevis. The procedure, using a large excess of gene-specific oligonucleotide primer and continuous incorporation of a radiolabeled deoxynucleoside triphosphate precursor, is more s...

journal_title：Molecular reproduction and development

authors： Brashears-Macatee S,Hinkley C,Perry M

更新日期：1990-01-01 00:00:00

abstract：:There are always more evidences indicating that 17beta-estradiol (E2) is to be necessary for normal male fertility. Here we report the expression of the most ubiquitously expressed member of the akt family of genes, akt1, in the lizard (Podarcis s. sicula) testis. We have used a nonmammalian vertebrate model (the liza...

journal_title：Molecular reproduction and development

authors： Russo M,Troncone G,Guarino FM,Angelini F,Chieffi P

更新日期：2005-05-01 00:00:00

abstract：:The expression of both inhibin alpha- and inhibin/activin beta A-subunit mRNA was examined in equine uteroplacental tissues collected during pregnancy (days 90 to 300). Northern blot analysis revealed that 5 transcripts (7.0, 4.1, 3.4, 2.6, 1.5 kb) of beta A-subunit were present, and the most abundantly expressed tran...

journal_title：Molecular reproduction and development

authors： Yamanouchi K,Hirasawa K,Hasegawa T,Ikeda A,Chang KT,Matsuyama S,Nishihara M,Miyazawa K,Sawasaki T,Tojo H,Tachi C,Takahashi M

更新日期：1997-08-01 00:00:00

abstract：:In the equine, the zona pellucida (ZP) is the major barrier to successful in vitro fertilization. Therefore the aim of our studies was to analyze species-specific features of the equine ZP in regard to structure and glycoprotein ZPB and ZPC expression sites during oocyte development and embryogenesis. The equine ZP re...

journal_title：Molecular reproduction and development

authors： Kölle S,Dubois CS,Caillaud M,Lahuec C,Sinowatz F,Goudet G

更新日期：2007-07-01 00:00:00

abstract：:Normal epididymal function, such as protein expression and secretion, is primarily regulated by testicular androgens and temperature. However, the role of spermatozoa in this critical process has never been studied. In order to determine whether sperm itself could regulate epididymal function, we have developed a cell...

journal_title：Molecular reproduction and development

authors： Reyes-Moreno C,Laflamme J,Frenette G,Sirard MA,Sullivan R

更新日期：2008-03-01 00:00:00