The molecular machinery of germ line specification.

abstract：:Amino acid analyses of nuclear basic proteins of an anuran amphibian, Rana catesbeiana, revealed that they are comprised of a full set of core histones and three types of lysine-rich, sperm-specific proteins. On the basis of their amino-acid compositions and partial amino-acid sequences of their trypsin-resistant core...

journal_title：Molecular reproduction and development

authors： Itoh T,Ausio J,Katagiri C

更新日期：1997-06-01 00:00:00

abstract：:Mammalian reproductive processes involve spermatogenesis, which occurs in the testis, and fertilization, which takes place in the female genital tract. Fertilization is a successive, multistep, and extremely complicated event that usually includes sperm survival in the uterus, sperm migration through the uterotubal ju...

journal_title：Molecular reproduction and development

authors： Xiong W,Wang Z,Shen C

更新日期：2019-08-01 00:00:00

abstract：:The objective of the present study was to investigate the ultrastructural consequences of vitrification of bovine oocytes at the metaphase II (MII) stage by the so-called "Open Pulled Straw" method. Oocytes were matured in vitro for 22 hr and cryopreserved by vitrification. After warming and additional 2 hr of culture...

journal_title：Molecular reproduction and development

authors： Hyttel P,Vajta G,Callesen H

更新日期：2000-05-01 00:00:00

abstract：:During the molecular cloning of the ovine testicular follicle-stimulating (FSH) receptor that couples to the Gs-type effector systems, we discovered novel cDNA clones that were highly homologous. Some of these clones contained an insert of 1,584 bp, which consisted of a divergent 3' region spliced with a 5' region tha...

journal_title：Molecular reproduction and development

authors： Yarney TA,Jiang L,Khan H,MacDonald EA,Laird DW,Sairam MR

更新日期：1997-12-01 00:00:00

abstract：:In this study, we cloned and characterized the follicle stimulating hormone receptor (Fshr) and luteinizing hormone receptor (Lhr) cDNAs of pejerrey Odontesthes bonariensis, a species with temperature-dependent sex determination (TSD), and analyzed their expression in relation to Fshb and Lhb subunits during gonadogen...

journal_title：Molecular reproduction and development

authors： Shinoda T,Miranda LA,Okuma K,Hattori RS,Fernandino JI,Yoshizaki G,Somoza GM,Strüssmann CA

更新日期：2010-06-01 00:00:00

abstract：:Fertilization causes mature oocytes or eggs to increase their concentrations of intracellular calcium ions (Ca²⁺) in all animals that have been examined, and such Ca²⁺ elevations, in turn, provide key activating signals that are required for non-parthenogenetic development. Several lines of evidence indicate that the ...

journal_title：Molecular reproduction and development

authors： Kashir J,Deguchi R,Jones C,Coward K,Stricker SA

更新日期：2013-10-01 00:00:00

abstract：:Hyperactivated motility was studied in guinea pig spermatozoa. In the presence of the local anesthetic procaine, a high number of sperm cells (64%) showed hyperactivation when incubated in minimal culture medium with pyruvate, lactate, and glucose. Hyperactivated motility was dependent on glucose in the medium. Sperm ...

journal_title：Molecular reproduction and development

authors： Mújica A,Neri-Bazan L,Tash JS,Uribe S

更新日期：1994-07-01 00:00:00

abstract：:In this study cytoskeletal antigens common to brushtail possum and tammar wallaby spermatozoa were characterised using a monoclonal antibody (PSA-10). Using indirect immunofluorescence, the PSA-10 antibody detected antigens predominantly associated with the midpiece and principal piece of mature, permeabilised marsupi...

journal_title：Molecular reproduction and development

authors： Harris MS,Rodger JC

更新日期：1998-08-01 00:00:00

abstract：:In the dasyurid marsupial, Sminthopsis crassicaudata, the mature spermatozoon has an inner homogeneous (C1) and a peripheral indented (C2) region. Using DNase-gold conjugates, and biotinylated genomic DNA probes, DNA was found to occur in both C1 and C2 regions. The morphogenesis of the spermatozoon nucleus was invest...

journal_title：Molecular reproduction and development

authors： Soon LL,Breed WG

更新日期：1996-02-01 00:00:00

abstract：:The aim of this study was to evaluate the role of adiponectin in the delayed embryonic development of Cynopterus sphinx. Adiponectin receptor (ADIPOR1) abundance was first observed to be lower during the delayed versus non-delayed periods of utero-embryonic unit development. The effects of adiponectin treatment on emb...

journal_title：Molecular reproduction and development

authors： Anuradha,Krishna A

更新日期：2014-12-01 00:00:00

abstract：:Myricetin is a natural flavonoid, particularly enriched in red wines, whose occurrence is widespread among plants. Despite extensive research, the beneficial effects of Myricetin on human health are still controversial. Here, we tested the estrogen-like effect of the phytoestrogen Myricetin on human ejaculated sperm b...

journal_title：Molecular reproduction and development

authors： Aquila S,Santoro M,De Amicis F,Guido C,Bonofiglio D,Lanzino M,Cesario MG,Perrotta I,Sisci D,Morelli C

更新日期：2013-02-01 00:00:00

abstract：:The present study examined the effect of elevated Ca(2+) concentration in fusion/activation medium on the fusion and development of fetal fibroblast nuclear transfer (NT) porcine embryos. Frozen-thawed and serum starved fetal fibroblasts were transferred into the perivitelline space of enucleated oocytes. Cell fusion ...

journal_title：Molecular reproduction and development

authors： Cheong HT,Park KW,Im GS,Lai L,Sun QY,Day BN,Prather RS

更新日期：2002-04-01 00:00:00

abstract：:In studies of somatic cell nuclear transfer (SCNT), the ability of factors within the oocyte to epigenetically reprogram transferred nuclei is essential for embryonic development of the clone to proceed. However, irregular patterns of X-chromosome inactivation, abnormal expression of imprinted genes, and genomic DNA h...

journal_title：Molecular reproduction and development

authors： Golding MC,Williamson GL,Stroud TK,Westhusin ME,Long CR

更新日期：2011-05-01 00:00:00

abstract：:Full-length cDNA of a novel mouse gene upregulated in late stages of spermatogenic cells was cloned from mouse testis using overlapping RT-PCR and RACE. The mRNA of the gene was expressed mainly in diplotene/pachytene spermatocytes, round and elongating spermatids. We named this gene as SRG-L (Spermatogenesis Related ...

journal_title：Molecular reproduction and development

authors： Ma Q,Wang H,Guo R,Wang H,Ge Y,Ma J,Xue S,Han D

更新日期：2006-09-01 00:00:00

abstract：:In mammals, Sry, Sox9, and M33 act as regulators at a chromatin level and promote the maturation of embryonic gonads into testes. Recently, it was shown that transcriptional regulation by DNA methylation plays crucial roles in gene expression during the differentiation and development of various cell types. To determi...

journal_title：Molecular reproduction and development

authors： Mizukami T,Kanai Y,Fujisawa M,Kanai-Azuma M,Kurohmaru M,Hayashi Y

更新日期：2008-06-01 00:00:00

abstract：:Oxygen consumption of preimplantation and early postimplantation mouse embryos has been measured using a novel noninvasive ultramicrofluorescence technique, based on an oil-soluble, nontoxic quaternary benzoid compound pyrene, whose fluorescence is quenched in the presence of oxygen. Pyruvate and glucose consumption, ...

journal_title：Molecular reproduction and development

authors： Houghton FD,Thompson JG,Kennedy CJ,Leese HJ

更新日期：1996-08-01 00:00:00

abstract：:Previously, we cloned and sequenced a sperm specific antigen, designated as HSS (EMBL nomenclature human sperm associated antigen 9: hSPAG9) from human testis (Shankar et al.: Biochem Biophys Res Commun 243:561-565, 1998). The present study was conducted to isolate baboon proteomic homologue in order to find out wheth...

journal_title：Molecular reproduction and development

authors： Shankar S,Mohapatra B,Verma S,Selvi R,Jagadish N,Suri A

更新日期：2004-10-01 00:00:00

abstract：:The present study was conducted to reveal effects of in ovo injection of nonsteroidal aromatase inhibitor (Fadrozole) or estradiol at day 3 of incubation on mRNA levels of P45017alphahydroxylase (P450c17), P450 aromatase (P450arom) and anti-Müllerian hormone (AMH) in the chicken gonads. The mRNA levels in the gonads a...

journal_title：Molecular reproduction and development

authors： Nishikimi H,Kansaku N,Saito N,Usami M,Ohno Y,Shimada K

更新日期：2000-01-01 00:00:00

abstract：:Among Sicilian stick insects, two hybridogenetic complexes have been discovered: Bacillus rossius-grandii benazzii and B. rossius-grandii grandii, which also produce androgenetic offspring. The egg maturation of the former is analyzed here through DAPI fluorometry, which, besides the assessment of the meiotic stages, ...

journal_title：Molecular reproduction and development

authors： Tinti F,Scali V

更新日期：1992-11-01 00:00:00

abstract：:In vitro culture of mammalian preimplantation embryos is associated with various developmental disorders such as retardation in development and cell damage. The molecular mechanisms underlying these processes are poorly understood. One of the possible reasons may be the unphysiologically high oxygen concentration used...

journal_title：Molecular reproduction and development

authors： Koerber S,Santos AN,Tetens F,Küchenhoff A,Fischer B

更新日期：1998-04-01 00:00:00

abstract：:Studies of ovine interferon-tau (oIFNtau) gene regulation, an anti-luteolytic factor produced by conceptuses of the ruminant ungulates, have been carried out, but a definitive mechanism for its spatial-temporal transcription has not been elucidated. Recently, specific binding regions for transcription factors AP-1 and...

journal_title：Molecular reproduction and development

authors： Xu N,Takahashi Y,Matsuda F,Sakai S,Christenson RK,Imakawa K

更新日期：2003-05-01 00:00:00

abstract：:The objective of this research was to evaluate if DNA hypomethylation in cells used as karyoplasts would improve development of bovine nuclear transplantation (NT) embryos. DNA from serum-fed (SF), serum-starved (SS), and 1, or 5 microM 5-azacytidine (5-aza-CR) treated cells was digested with a methylation sensitive e...

journal_title：Molecular reproduction and development

authors： Jones KL,Hill J,Shin TY,Lui L,Westhusin M

更新日期：2001-10-01 00:00:00

abstract：:The aim of the present study was to investigate the implication of protein kinase C (PKC) in the mouse egg activation process. We used OAG (1-oleoyl-2-acetyl-sn-glycerol) as a PKC activator, calphostin C as a specific PKC inhibitor, and the calcium ionophore A23187 as a standard parthenogenetic agent. The exposure of ...

journal_title：Molecular reproduction and development

authors： Colonna R,Tatone C,Francione A,Rosati F,Callaini G,Corda D,Di Francesco L

更新日期：1997-10-01 00:00:00

abstract：:In this study we have examined the effect of insulin like growth factor I (IGF-I) and its interaction with gonadotropins in the presence or absence of granulosa cell coculture on in vitro oocyte maturation (IVM) and their subsequent embryonic development in buffalo. We also have examined the role of IGF-I alone or in ...

journal_title：Molecular reproduction and development

authors： Pawshe CH,Rao KB,Totey SM

更新日期：1998-03-01 00:00:00

abstract：:Mouse oocyte development in vitro has been studied in the past several years, but no evidence showed that the fertilizable oocytes could be obtained from the fetal mouse germ cells before the formation of the primordial follicles. In this study, an efficient and simple method has been established to obtain the mature ...

journal_title：Molecular reproduction and development

authors： Shen W,Li L,Zhang D,Pan Q,Ding M,Deng H

更新日期：2006-10-01 00:00:00

abstract：:A porcine interleukin-6 (pIL-6) cDNA has been cloned from pig spleen cDNA library to provide information that would allow us to study IL-6 mRNA expression during pregnancy of several domestic Artiodactyla. The cDNA is 1058 bp long and with a single open reading frame that encodes a 212 amino acid polypeptide with 28-r...

journal_title：Molecular reproduction and development

authors： Mathialagan N,Bixby JA,Roberts RM

更新日期：1992-08-01 00:00:00

abstract：:Estrogen plays an important role in the human reproductive system and its action is mediated mainly by two specific receptors: α (ERα) and β (ERβ). There are polymorphic variants in both ER genes, and studies showed their association with reproductive outcomes. We aimed to determine the distribution of ERα and ERβ gen...

journal_title：Molecular reproduction and development

authors： Bianco B,Peluso C,Gava MM,Ghirelli-Filho M,Lipay MV,Lipay MA,Christofolini DM,Barbosa CP

更新日期：2011-09-01 00:00:00

abstract：:Follicle-stimulating hormone (FSH) regulates folliculogenesis in the ovary and spermatogenesis in the testis via specific, high affinity membrane-bound receptors (FSHR). To assess the role of FSHR gene expression in regulating expression of FSHR protein in the plasma membrane, the effects of a porcine FSHR cDNA antise...

journal_title：Molecular reproduction and development

authors： Zhu C,Wang Y,Nixon MD,La Barbera AR

更新日期：2003-06-01 00:00:00

abstract：:We have previously reported metabolic cooperation between Sertoli and peritubular myoid cells in terms of synthesis of one of the main testicular extracellular matrix (ECM) constituents, glycosaminoglycans (GAG). This study concerns Sertoli cell ECM-peritubular myoid cell interactions in terms of GAG synthesis. We hav...

journal_title：Molecular reproduction and development

authors： Raychoudhury SS,Blackshaw AW,Irving MG

更新日期：1993-06-01 00:00:00

abstract：:An exogenous supply of hematopoietic cytokines is essential for maintaining murine embryonic stem (ES) cells in a proliferative yet undifferentiated state. Recently, it was demonstrated that hematopoietic cytokines utilize the gp130 signal transduction pathway to maintain this phenotype, although their involvement tow...

journal_title：Molecular reproduction and development

authors： Moore K,Piedrahita JA

更新日期：1996-10-01 00:00:00