Abstract:
:In this study we have examined the effect of insulin like growth factor I (IGF-I) and its interaction with gonadotropins in the presence or absence of granulosa cell coculture on in vitro oocyte maturation (IVM) and their subsequent embryonic development in buffalo. We also have examined the role of IGF-I alone or in combination with gonadotropins on DNA synthesis, steroidogenesis, and protein synthesis of cumulus-oocytes complexes (COCs) and granulosa cells. Results showed that IGF-I stimulates oocytes maturation in a dose-dependent manner, with maximal effect at a dose of 100 ng/ml (P < 0.05). IGF-I showed positive interaction with follicle-stimulating hormone (FSH) in the presence or absence of granulosa cells on meiotic maturation and synergistically enhanced DNA synthesis, protein synthesis, and steroidogenesis in the presence of granulosa cells. This synergistic effect is mainly caused by the increase of IGF-I receptors in granulosa cells by FSH, as evident by [125I]IGF-I binding study. Luteinizing hormone (LH), however, was found to suppress IGF-I and IGF-I + FSH stimulated oocyte maturation. Addition of LH to cultures containing IGF-I + FSH, on the contrary, caused a significant increase in oocyte maturation when cocultured with granulosa cells. Addition of IGF-I during IVM significantly improve cleavage and blastocyst development rate over the control group. However, there was no cumulative effect when IGF-I and gonadotropins were present together. Addition of granulosa cells during IVM, however, enhanced blastocyst development in the IGF-I + FSH and IGF-I + FSH + LH groups. Our results demonstrated that IGF-I is a major follicular factor responsible for stimulating oocyte maturation in the buffalo. Interaction between IGF-I and FSH suggests that they seem to act synergistically as an autocrine and paracrine regulator of granulosa cells and therefore together promote mitosis, steroidogenesis, and protein synthesis.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Pawshe CH,Rao KB,Totey SMdoi
10.1002/(SICI)1098-2795(199803)49:3<277::AID-MRD8>subject
Has Abstractpub_date
1998-03-01 00:00:00pages
277-85issue
3eissn
1040-452Xissn
1098-2795pii
10.1002/(SICI)1098-2795(199803)49:3<277::AID-MRD8>journal_volume
49pub_type
杂志文章abstract::Acrosin is thought to fulfill several different roles in fertilization including that of a serine protease and in secondary zona pellucida (ZP) binding. However, acrosin's importance as a fertilization protein has been questioned. Especially since it was discovered that acrosin knockout mice are fertile. In this study...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
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journal_title:Molecular reproduction and development
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pub_type: 杂志文章
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更新日期:1999-10-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22505
更新日期:2015-09-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080370207
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
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journal_title:Molecular reproduction and development
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doi:10.1002/(SICI)1098-2795(199704)46:4<443::AID-MRD1>
更新日期:1997-04-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
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更新日期:2008-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:1988-01-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22309
更新日期:2014-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199712)48:4<458::AID-MRD6>
更新日期:1997-12-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20916
更新日期:2009-01-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22801
更新日期:2017-05-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080290107
更新日期:1991-05-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080380212
更新日期:1994-06-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20189
更新日期:2005-01-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20548
更新日期:2006-11-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.10234
更新日期:2003-04-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2008-05-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080330404
更新日期:1992-12-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/1098-2795(200012)57:4<406::AID-MRD13>3.0.C
更新日期:2000-12-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1010
更新日期:2001-05-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.10395
更新日期:2004-02-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20522
更新日期:2007-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:1996-06-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080340406
更新日期:1993-04-01 00:00:00
abstract::Members of the Binder of SPerm (BSP) superfamily have been identified in both human and mouse epididymis. These proteins are known to bind sperm membrane and promote sperm capacitation. Studies suggest that BSPH2 might play a different role in sperm functions from its counterparts; however, the role of BSPH2 remains m...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.23039
更新日期:2018-08-01 00:00:00
abstract::In vitro growth of preantral follicles has the potential to produce considerable numbers of competent oocytes for use in medicine, agriculture, and even wildlife conservation. The critical regulatory role of growth factors and hormones in the development of preantral follicles has been established. This study investig...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22131
更新日期:2013-01-01 00:00:00