Abstract:
:Under the influence of progesterone, the porcine uterus synthesizes plasmin/trypsin inhibitor (PTI), a low molecular weight protein (M(r) approximately 14,000) belonging to the Kunitz family of proteinase inhibitors. Here it is demonstrated that mRNA for the same protein is produced by the developing trophoblast during early pregnancy and by the placenta throughout gestation. The transcript for PTI was represented in approximately 0.1% of total phage plaques of a day 13-17 porcine conceptus cDNA library. It shared 99% nucleotide sequence identity with the cDNA isolated from the uterine library and encoded a 112-amino-acid protein identical to the uterine-produced PTI, which has a well-defined Kunitz domain comprised of 64 residues at its amino terminus. Northern analysis and in situ hybridization studies confirmed that expression of PTI by the conceptus begins as early as day 10 of pregnancy and is continued in placental tissues until at least day 90 of gestation. Expression was trophoblast specific at day 20 of gestation, as in situ hybridization detected no mRNA in the embryo. The pattern of PTI expression during pregnancy is consistent with a role either in controlling trophoblast invasiveness or in inhibiting proteinases with trypsin-like specificity released by immune or inflammatory cells.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Duffy JY,Stallings-Mann ML,Trout WE,Roberts RMdoi
10.1002/(SICI)1098-2795(199704)46:4<443::AID-MRD1>subject
Has Abstractpub_date
1997-04-01 00:00:00pages
443-9issue
4eissn
1040-452Xissn
1098-2795pii
10.1002/(SICI)1098-2795(199704)46:4<443::AID-MRD1>journal_volume
46pub_type
杂志文章abstract::The murine intracisternal A particle (IAP) proviral elements are expressed at low levels in undifferentiated F9 embryonal carcinoma cells but are highly expressed when F9 cells are induced to differentiate into parietal endoderm-like cells. IAP elements are also expressed in parietal endoderm-like PYS-2 cells. We prev...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
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journal_title:Molecular reproduction and development
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doi:10.1002/mrd.1080380103
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journal_title:Molecular reproduction and development
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pub_type: 杂志文章,评审
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pub_type: 杂志文章
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更新日期:1996-05-01 00:00:00
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doi:10.1002/mrd.20038
更新日期:2004-04-01 00:00:00
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更新日期:2003-06-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
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更新日期:1994-12-01 00:00:00
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更新日期:1996-04-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080400108
更新日期:1995-01-01 00:00:00
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更新日期:2018-11-01 00:00:00
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pub_type: 杂志文章
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