Abstract:
:In the dasyurid marsupial, Sminthopsis crassicaudata, the mature spermatozoon has an inner homogeneous (C1) and a peripheral indented (C2) region. Using DNase-gold conjugates, and biotinylated genomic DNA probes, DNA was found to occur in both C1 and C2 regions. The morphogenesis of the spermatozoon nucleus was investigated using ultrastructural and cytochemical studies. Spermiogenesis was divided into 15 steps. By step 10, condensation of the C1 region was complete, and at the caudal extremity of the spermatid nucleus, the nuclear envelope enclosed an electron-lucent space. This space and the surrounding nuclear envelope became very enlarged at step 11. At this stage, a plate of approximately 70 nm in thickness was present along the caudal segment of the C1 region; this "nuclear mantle" did not bind DNase-gold conjugates but stained for lysine-rich proteins using alcoholic phosphotungstic acid. Chromatin condensation resumed at step 12 with the appearance of spherical chromatin structures peripheral to the C1 chromatin. These structures then partially coalesced and the indentations of the C2 region were observed. The expanded nuclear envelope at the caudal extremity persisted in caput epididymal spermatozoa. Spherical inclusions within it did not bind to DNase-gold conjugates but stained for lysine-rich proteins. As the sperm traveled down the epididymis, these inclusions amassed near the nuclear pores and were then removed from the nucleus. In addition, the nuclear mantle was found to have disappeared by the time the spermatozoa reached the corpus epididymidis.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Soon LL,Breed WGdoi
10.1002/(SICI)1098-2795(199602)43:2<217::AID-MRD11subject
Has Abstractpub_date
1996-02-01 00:00:00pages
217-27issue
2eissn
1040-452Xissn
1098-2795pii
10.1002/(SICI)1098-2795(199602)43:2<217::AID-MRD11journal_volume
43pub_type
杂志文章abstract::In studies of somatic cell nuclear transfer (SCNT), the ability of factors within the oocyte to epigenetically reprogram transferred nuclei is essential for embryonic development of the clone to proceed. However, irregular patterns of X-chromosome inactivation, abnormal expression of imprinted genes, and genomic DNA h...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.21306
更新日期:2011-05-01 00:00:00
abstract::A porcine interleukin-6 (pIL-6) cDNA has been cloned from pig spleen cDNA library to provide information that would allow us to study IL-6 mRNA expression during pregnancy of several domestic Artiodactyla. The cDNA is 1058 bp long and with a single open reading frame that encodes a 212 amino acid polypeptide with 28-r...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080320404
更新日期:1992-08-01 00:00:00
abstract::Carp hyosophorin (HSP) is purified from oocytes. It is a highly glycosylated protein (10% protein and 90% carbohydrate) of high molecular weight (>100 kDa) and is localized in the cortical granules of oocytes. During cortical reaction carp HSP is exocytosed into the perivitelline space and is rapidly cleaved to the lo...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199903)52:3<288::AID-MRD6>
更新日期:1999-03-01 00:00:00
abstract::Estrogen receptor mRNA expression was detected in the head and tail parts of the human epididymis by means of reverse transcription polymerase chain reaction (RT-PCR). Immunocytochemical labelling showed that ciliate and nonciliate cells from the epithelium of the efferent ductules possessed strong to moderate nuclear...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199708)47:4<448::AID-MRD12
更新日期:1997-08-01 00:00:00
abstract::The aim of the present study is to investigate the effect of insulin-like growth factor (IGF-I) on the development of cultured rat pre-antral follicles. Pre-antral follicles with a diameter between 140 and 160 microm are mechanically isolated from ovaries of 10-day-old rats and cultured in groups for 6 days in FSH and...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/1098-2795(200103)58:3<287::AID-MRD7>3.0.CO
更新日期:2001-03-01 00:00:00
abstract::A spontaneously established porcine granulosa cell line (PGC-2) was cloned through the continuous culturing of primary granulosa cells collected from equine chorionic gonadotropin (eCG)-treated prepubertal gilts. This established cell line has undergone approximately 100 passages and shows contact-inhibition of growth...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199611)45:3<299::AID-MRD6>
更新日期:1996-11-01 00:00:00
abstract::Sperm from the decapod crustacean Rhynchocinetes typus undergo dramatic shape changes as they pass from the vas deferens to seawater and interact with the oocyte envelopes. Using FITC-phalloidin and antitubulin antibodies, we were able to localize microfilaments and a tubulin-like protein in R. typus spermatozoon. Mic...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080280410
更新日期:1991-04-01 00:00:00
abstract::Primordial germ cells (PGCs) are useful for producing transgenic chickens and preserving genetic material in avian species. In this study, we investigated the in vitro differentiation potential of chicken PGCs into different cell types. For differentiation into adipocytes, chicken PGCs were cultured for 21 days in ind...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.21224
更新日期:2010-09-01 00:00:00
abstract::In vitro growth of preantral follicles has the potential to produce considerable numbers of competent oocytes for use in medicine, agriculture, and even wildlife conservation. The critical regulatory role of growth factors and hormones in the development of preantral follicles has been established. This study investig...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22131
更新日期:2013-01-01 00:00:00
abstract::In the mammalian testis the cytoplasmic beta and gamma actins are expressed in all stages of germ-cell differentiation, whereas gamma enteric actin is expressed in germ cells solely in postmeiotic stages. Northern blot analysis of mouse testicular RNAs reveals actin mRNAs of about 2.1, 1.5, and 1.4 kB. The 2.1-kB mRNA...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199606)44:2<141::AID-MRD1>
更新日期:1996-06-01 00:00:00
abstract::Pannexins (Panxs) are channel-forming proteins that have homology to the invertebrate gap junction proteins, the innexins. These proteins form membrane channels implicated in ATP release. To evaluate the role of Panxs in the male reproductive tract, we investigated the distribution and regulation of Panx1 and 3 in the...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.21280
更新日期:2011-02-01 00:00:00
abstract::The aim of this study was to examine the expression and regulation of peroxisome proliferator-activated receptor (PPAR) PPARdelta gene in mouse uterus during early pregnancy by in situ hybridization and immunohistochemistry. PPARdelta expression under pseudopregnancy, delayed implantation, hormonal treatment, and arti...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.10348
更新日期:2003-11-01 00:00:00
abstract::The expression of both inhibin alpha- and inhibin/activin beta A-subunit mRNA was examined in equine uteroplacental tissues collected during pregnancy (days 90 to 300). Northern blot analysis revealed that 5 transcripts (7.0, 4.1, 3.4, 2.6, 1.5 kb) of beta A-subunit were present, and the most abundantly expressed tran...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199708)47:4<363::AID-MRD2>
更新日期:1997-08-01 00:00:00
abstract::The cumulus cells that surround the mammalian oocyte become dispersed following the preovulatory surge of the pituitary gonadotropin, luteinizing hormone (LH). We have examined cumulus-oocyte complexes of PMSG-primed immature rats before and at 1, 2, 3, 4, 6, and 8 hr after injection of human chorionic gonadotropin (h...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080280313
更新日期:1991-03-01 00:00:00
abstract::The genome of germline committed cells is thought to be protected by mechanisms of transcriptional silencing, posing a barrier to transgenesis using cultured germline cells. We found that selection for transgene integration into the primordial germ cell genome required that the transgenes be flanked by the chicken bet...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20859
更新日期:2008-07-01 00:00:00
abstract::The aim of this study is to examine the effect of bovine oocyte maturation, fertilization or culture in vivo or in vitro on the proportion of oocytes reaching the blastocyst stage, and on blastocyst quality as measured by survival following vitrification. In Experiment 1, 4 groups of oocytes were used: (1) immature oo...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1153
更新日期:2002-02-01 00:00:00
abstract::Fresh cytosols extracted from unfertilized amphibian eggs contain a cytostatic factor (CSF) which arrests the cell cycle at metaphase when microinjected into cleaving blastomeres. This CSF is sensitive to Ca2+, and is designated primary CSF (1 degree CSF). During storage of Ca(2+)-containing cytosols at 2 degrees C, s...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199707)47:3<302::AID-MRD9>
更新日期:1997-07-01 00:00:00
abstract::Gonadotropin-Releasing Hormones (GnRHs) are decapeptides well known to regulate the reproductive cycle. They are expressed not only in the brain, but also in other tissues including the gonads. It is believed that they may be involved in the endocrine and paracrine regulation of the reproductive cycle. To date, two fo...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.10065
更新日期:2002-05-01 00:00:00
abstract::Various thiol compounds are known to improve cytoplasmic and/or nuclear maturation of oocytes in vitro. The present study examined the effects of two thiol compounds, cysteine (0.1, 0.5, and 1.0 mM) and cysteamine (50, 100, and 200 microM), on cytoplasmic and nuclear maturation of canine oocytes. Oocytes collected fro...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20674
更新日期:2007-09-01 00:00:00
abstract::Altering incubation temperature during embryogenesis has an impact on chicken embryo growth, but the underlying molecular mechanisms are not understood; the present study was performed to address these changes. Broiler eggs were incubated at low (36.8°C), control (37.8°C), and high (38.8°C) temperatures between Embryo...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22596
更新日期:2016-01-01 00:00:00
abstract::Human ejaculate spermatozoa contain multiple mRNA species carried over from earlier stages in spermatogenesis. To date, gene-specific RT-PCR or in situ hybridization has detected transcripts for beta-actin, heat shock proteins (HSP) 70 and 90, protamines (PRM) 1 and 2, transition protein (TNp) 2, HLA II, beta-integrin...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(200006)56:2+<259::AID-MRD1
更新日期:2000-06-01 00:00:00
abstract::We produced four transgenic founder rats (F0) by introducing into rat embryos a fusion gene, which consisted of rat growth hormone (GH) promoter containing with four copies of thyroid hormone response element (TRE) and antisense cDNA sequences for rat GH. This transgene promoter directed 2.8-fold stimulation of CAT ge...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080360109
更新日期:1993-09-01 00:00:00
abstract::We have previously shown that bovine oocytes parthenogenetically activated after 40 hours (hr) of in vitro maturation proceed through the cell cycle faster than those after 20 hr of maturation. In the present study, we used this model of different speed of nuclear progression to investigate the correlation of two hall...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.10072
更新日期:2002-05-01 00:00:00
abstract::Previous studies from our laboratory have identified MPS, a 100-kDa protein, as the major phosphoprotein substrate of caprine sperm ecto-cyclic AMP independent protein kinase. In this study the isolated (32)P-labelled MPS has been incorporated into mature caprine (Capra indicus) cauda-epididymal spermatozoa with the h...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20854
更新日期:2008-07-01 00:00:00
abstract::Sry expression is essential for initiating male sex differentiation, and the expression occurs only during a restricted period in the developing gonad. It is thought that Sry is part of a pathway of genes that regulate sex determination. Although the interactions of several genes with Sry expression have been suggeste...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.10278
更新日期:2003-04-01 00:00:00
abstract::Three monoclonal antibodies against human protamines were obtained by immunization with total human basic nuclear proteins or purified protamine HP3. The specificity of antibodies was assessed by enzyme-linked immunosorbent assay (ELISA) and Western blot. They recognized three distinct epitopes: One was specific for t...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080360115
更新日期:1993-09-01 00:00:00
abstract::An in vitro superfusion method was used to test sex hormone release from different kinds of ovarian follicle (growing follicles, postovulatory follicles, and atretic follicles) in the lizard Podarcis sicula sicula. Sex hormone output changes with the stage of follicle evolution and sexual cycle. Previtellogenetic foll...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080350307
更新日期:1993-07-01 00:00:00
abstract::The non-neuronal monoamine transporters (OCT1, OCT2, EMT, and PMAT) play a key role in the clearance of monoamines from extracellular compartments. In a previous report we described endometrial distribution and cyclic variation of the vesicular monoamine transporter (VMAT2) mRNA and the neuronal norepinephrine transpo...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20697
更新日期:2007-10-01 00:00:00
abstract::In Ciona intestinalis, sperm penetration through the egg vitelline coat is an essential event of fertilization. We investigated whether trypsin- and chymotrypsin-like enzymes are involved in this event. Inhibitors and peptide substrates for chymotrypsin-like enzymes blocked the overall process of fertilization in a co...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080260405
更新日期:1990-08-01 00:00:00
abstract::Previously, we cloned and sequenced a sperm specific antigen, designated as HSS (EMBL nomenclature human sperm associated antigen 9: hSPAG9) from human testis (Shankar et al.: Biochem Biophys Res Commun 243:561-565, 1998). The present study was conducted to isolate baboon proteomic homologue in order to find out wheth...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20164
更新日期:2004-10-01 00:00:00