Follicle-oocyte atresia and temporal taphonomy in cold-stored domestic cat ovaries.

abstract：:Zona pellucida (ZP) glycoproteins have been proposed as candidate antigens for an immunocontraceptive vaccine. The efficacy of such a vaccine has to be evaluated in nonhuman primates, thus necessitating the characterization of their ZP glycoproteins. A bonnet monkey (Macaca radiata) ovarian cDNA lambda gt11 library wa...

journal_title：Molecular reproduction and development

authors： Jethanandani P,Santhanam R,Gupta SK

更新日期：1998-06-01 00:00:00

abstract：:The murine intracisternal A particle (IAP) proviral elements are expressed at low levels in undifferentiated F9 embryonal carcinoma cells but are highly expressed when F9 cells are induced to differentiate into parietal endoderm-like cells. IAP elements are also expressed in parietal endoderm-like PYS-2 cells. We prev...

journal_title：Molecular reproduction and development

authors： Kamat JP,Basu A,Satyamoorthy K,Xu MQ,Showe L,Howe CC

更新日期：1995-05-01 00:00:00

abstract：:Studies of ovine interferon-tau (oIFNtau) gene regulation, an anti-luteolytic factor produced by conceptuses of the ruminant ungulates, have been carried out, but a definitive mechanism for its spatial-temporal transcription has not been elucidated. Recently, specific binding regions for transcription factors AP-1 and...

journal_title：Molecular reproduction and development

authors： Xu N,Takahashi Y,Matsuda F,Sakai S,Christenson RK,Imakawa K

更新日期：2003-05-01 00:00:00

abstract：:We previously showed that, during mouse oocyte maturation, specific maternal mRNAs (actins) are deadenylated, while others (hypoxanthine phosphoribosyltransferase:HPRT) are adenylated. As in other systems, these changes can be correlated with changes in translational activities. Maturation-specific polyadenylation in ...

journal_title：Molecular reproduction and development

authors： Paynton BV,Bachvarova R

更新日期：1994-02-01 00:00:00

abstract：:Fragmentation occurs during early developmental stages of electrically activated oocytes and nuclear transfer (NT) embryos. It might contribute to the low developmental rate of porcine NT embryos. The present study was conducted to investigate whether the addition of sugars such as sorbitol or sucrose suppresses fragm...

journal_title：Molecular reproduction and development

authors： Im GS,Yang BS,Lai L,Liu Z,Hao Y,Prather RS

更新日期：2005-06-01 00:00:00

abstract：:Glycoprotein hormone receptors contain large extracellular domains encoded by multiple exons that can be alternatively spliced. Using human ovarian surface epithelium, we cloned two new splice variants of the human follicle-stimulating hormone receptor (FSH-R) gene, hFSH-R2 and hFSH-R3. The hFSH-R2 splice variant diff...

journal_title：Molecular reproduction and development

authors： Zhou J,Huang Y,Li L,Zhu L,Zhang D,Zhang S,Chen Y

更新日期：2013-11-01 00:00:00

abstract：:Testis-specific protein kinases are important because of their potential role in spermiogenesis, sperm maturation, and sperm function. In the present study, a novel serine-threonine kinase with high identity to human serine-threonine kinase 31 (STK31) was cloned from equine testis and expression of the protein was cha...

journal_title：Molecular reproduction and development

authors： Sabeur K,Ball BA,Corbin CJ,Conley A

更新日期：2008-05-01 00:00:00

abstract：:Using alpha and beta spectrin mammalian antibodies on Western blotting, we demonstrated that lizard ovarian follicles contain two isoforms of alpha spectrin, Mr 94 and 134 kDa, and a 230 kDa beta spectrin, and that their pattern modifies in relation to pyriform cell differentiation. In fact, a positive immunoreaction ...

journal_title：Molecular reproduction and development

authors： Ricchiari L,Carmela VM,Marina P,Rosa C,Annamaria L,Piero A

更新日期：2004-01-01 00:00:00

abstract：:In pigs, the morphology and cell number of in vitro-produced blastocysts are inferior to those of their in vivo counterparts. The objective of this study was to increase developmental competence and to gain an understanding of cell allocation in blastocysts derived from the aggregation of four-cell stage porcine embry...

journal_title：Molecular reproduction and development

authors： Lee SG,Park CH,Choi DH,Kim HS,Ka HH,Lee CK

更新日期：2007-11-01 00:00:00

abstract：:Demecolcine-induced enucleation (IE) of mouse oocytes has been shown to improve development to term of cloned mice. In this study, we characterized the kinetics and morphological progression of bovine oocytes subjected to IE, and evaluated their ability to support embryo development to the blastocyst stage after nucle...

journal_title：Molecular reproduction and development

authors： Russell DF,Ibáñez E,Albertini DF,Overström EW

更新日期：2005-10-01 00:00:00

abstract：:Activation of the mammalian egg results in cortical reaction (CR), which is correlated with an increase in intracellular Ca2+ concentration and PKC activation. The CR is a gradual rather then an "all or none" response, and can be regulated by different concentrations of parthenogenetic activators. To evaluate the biol...

journal_title：Molecular reproduction and development

authors： Raz T,Skutelsky E,Amihai D,Hammel I,Shalgi R

更新日期：1998-11-01 00:00:00

abstract：:Prostaglandins could be involved in various aspects of final differentiation of ovarian follicles. Prostaglandins are generated by the cyclooxygenase (cox) pathway. Until now, the expression pattern of isoforms cox-1 and cox-2 of cyclooxygenase in bovine cumulus-oocyte complexes (COCs) was unknown. Using immunodetecti...

journal_title：Molecular reproduction and development

authors： Nuttinck F,Reinaud P,Tricoire H,Vigneron C,Peynot N,Mialot JP,Mermillod P,Charpigny G

更新日期：2002-01-01 00:00:00

abstract：:Epigenetics includes the study of external factors that can influence the expression of genes by altering the accessibility of DNA through methylation. To investigate the epigenetic influence of season, sperm head shape, and semen storage on placental and fetal tissues, pregnancies were generated in the summer or wint...

journal_title：Molecular reproduction and development

authors： Rempel LA,Krautkramer MM,Parrish JJ,Miles JR

更新日期：2019-04-01 00:00:00

abstract：:Changes in the level of transcriptional activity in 32-cell stage morula nuclei were studied after blastomere electrofusion to enucleated oocytes. Nuclear transplant recipients were pulse labelled with 3H-uridine during cultivation in vitro, embryos were then fixed and processed for autoradiography and electron micros...

journal_title：Molecular reproduction and development

authors： Kanka J,Hozák P,Heyman Y,Chesné P,Degrolard J,Renard JP,Fléchon JE

更新日期：1996-02-01 00:00:00

abstract：:Normal epididymal function, such as protein expression and secretion, is primarily regulated by testicular androgens and temperature. However, the role of spermatozoa in this critical process has never been studied. In order to determine whether sperm itself could regulate epididymal function, we have developed a cell...

journal_title：Molecular reproduction and development

authors： Reyes-Moreno C,Laflamme J,Frenette G,Sirard MA,Sullivan R

更新日期：2008-03-01 00:00:00

abstract：:Sequential culture and coculture are two methods of improving the development of preimplantation embryos in vitro. Direct comparison of the efficiency of these methods is limited. Proliferation and apoptosis determine the total number of blastomere in preimplantation embryo, which is a sensitive parameter for evaluati...

journal_title：Molecular reproduction and development

authors： Xu JS,Chan ST,Lee WW,Lee KF,Yeung WS

更新日期：2004-05-01 00:00:00

abstract：:Bicarbonate/CO2 is believed to be the key in vitro effector of sperm capacitation, a process which induces major changes in the sperm plasma membrane in preparation for fertilization. In a flow cytometric study, we examined the effect of bicarbonate on boar spermatozoa using merocyanine, an impermeant lipophilic probe...

journal_title：Molecular reproduction and development

authors： Harrison RA,Ashworth PJ,Miller NG

更新日期：1996-11-01 00:00:00

abstract：:Carp hyosophorin (HSP) is purified from oocytes. It is a highly glycosylated protein (10% protein and 90% carbohydrate) of high molecular weight (>100 kDa) and is localized in the cortical granules of oocytes. During cortical reaction carp HSP is exocytosed into the perivitelline space and is rapidly cleaved to the lo...

journal_title：Molecular reproduction and development

authors： Tsao CC,Huang FL,Chang YS

更新日期：1999-03-01 00:00:00

abstract：:The aim of this study was to evaluate the role of adiponectin in the delayed embryonic development of Cynopterus sphinx. Adiponectin receptor (ADIPOR1) abundance was first observed to be lower during the delayed versus non-delayed periods of utero-embryonic unit development. The effects of adiponectin treatment on emb...

journal_title：Molecular reproduction and development

authors： Anuradha,Krishna A

更新日期：2014-12-01 00:00:00

abstract：:Insulators are located at the boundaries of differentially regulated genes and delimit their interactions by establishing independent chromatin structures. Recently, an insulator sequence has been found in the 5'-flanking region of arylsulfatase (ARS) gene from sea urchin. To investigate functional conservation of thi...

journal_title：Molecular reproduction and development

authors： Takada T,Iida K,Akasaka K,Yasue H,Torii R,Tsujimoto G,Taira M,Kimura H

更新日期：2000-11-01 00:00:00

abstract：:Regulation of protein tyrosine phosphorylation is required for sperm capacitation and oocyte fertilization. The objective of the present work was to study the role of the calcium-sensing receptor (CaSR) on protein tyrosine phosphorylation in boar spermatozoa under capacitating conditions. To do this, boar spermatozoa ...

journal_title：Molecular reproduction and development

authors： Macías-García B,García-Marín LJ,Bragado MJ,González-Fernández L

更新日期：2019-07-01 00:00:00

abstract：:Under the influence of gonadotropins or growth factors, a close cooperation develops between cumulus cells and the oocyte that is implicated in transmitting signals involved in maintaining or releasing the meiotic arrest in the oocyte. While cyclic adenosine 5'-monophosphate (cAMP) is a key molecule in maintaining the...

journal_title：Molecular reproduction and development

authors： Silvestre F,Boni R,Fissore RA,Tosti E

更新日期：2011-10-01 00:00:00

abstract：:The internally fertilizing primitive frog Ascaphus truei (family Ascaphidae) from the Pacific Northwest is the only frog with an intromittent organ. The more advanced neobatrachian frog Eleutherodactylus coqui (family Leptodactylidae) from Puerto Rico has secondarily acquired internal fertilization but mates by cloaca...

journal_title：Molecular reproduction and development

authors： Kasinsky HE,Frehlick LJ,Su HW,Ausio J

更新日期：2005-03-01 00:00:00

abstract：:The neomycin-resistant gene (neo(r)) is probably the most commonly used selectable marker gene in gene targeting and gene transfection research. In this study, the neo(r) gene construct was introduced into in vitro cultured goat foetal fibroblast cells (IV-5), and the cells were selected with 900 microg/ml G418. The G...

journal_title：Molecular reproduction and development

authors： Zou X,Wang Y,Cheng Y,Yang Y,Ju H,Tang H,Shen Y,Mu Z,Xu S,Du M

更新日期：2002-02-01 00:00:00

abstract：:Full-length cDNA of a novel mouse gene upregulated in late stages of spermatogenic cells was cloned from mouse testis using overlapping RT-PCR and RACE. The mRNA of the gene was expressed mainly in diplotene/pachytene spermatocytes, round and elongating spermatids. We named this gene as SRG-L (Spermatogenesis Related ...

journal_title：Molecular reproduction and development

authors： Ma Q,Wang H,Guo R,Wang H,Ge Y,Ma J,Xue S,Han D

更新日期：2006-09-01 00:00:00

abstract：:Temporal pattern of expression of Cu/Zn and Mn superoxide dismutases (SODs) was investigated in bovine oocytes and embryos produced in vitro in two different culture conditions and in vivo after superovulation. SODs were examined at a transcriptional level in single oocytes and embryos by reverse transcriptase-polymer...

journal_title：Molecular reproduction and development

authors： Lequarré AS,Feugang JM,Malhomme O,Donnay I,Massip A,Dessy F,Van Langendonckt A

更新日期：2001-01-01 00:00:00

abstract：:In the rat, in response to blastocyst implantation, stromal cells of the endometrium proliferate and differentiate into decidual cells, forming the decidua. After reaching its maximum development, the decidua undergoes regression. This phenomenon appears to be due to an active process involving apoptosis. As there is ...

journal_title：Molecular reproduction and development

authors： Almeida A,Correia-da-Silva G,Cepa M,Bell SC,Teixeira NA

更新日期：2007-03-01 00:00:00

abstract：:We compared the results of intracytoplasmic sperm injection (ICSI) that leads to full term development of hybrid (B6C3F1 and B6D2F1) and inbred (C57BL/6) mouse embryos. Although fertilization and pre-implantation development of C57BL/6 eggs were similar to those of F1 hybrid eggs, post-implantation development of the ...

journal_title：Molecular reproduction and development

authors： Kawase Y,Iwata T,Toyoda Y,Wakayama T,Yanagimachi R,Suzuki H

更新日期：2001-09-01 00:00:00

abstract：:Irregular uterine bleeding is a major side effect of long-acting progestogen-only contraceptives in women, and is the primary reason women discontinue their use. In this study, a mouse model of endometrial breakdown was established using a subcutaneous progesterone implant to understand how irregular bleeding begins. ...

journal_title：Molecular reproduction and development

authors： Zhang GH,Cui LJ,Li AY,Zhang JP,Liu Y,Zhao JS,Xu XB,He B,Wang JD,Chu L,Li YF

更新日期：2016-09-01 00:00:00

abstract：:The influence of somatic cells on oocyte penetrability was studied during in vitro maturation. Four experiments were carried out. In the first, pig oocytes were fertilized in vitro immediately after collection (immature oocytes) or after being cultured for 44 hr with cumulus cells connected to the whole wall of the ex...

journal_title：Molecular reproduction and development

authors： Galeati G,Modina S,Lauria A,Mattioli M

更新日期：1991-05-01 00:00:00