Abstract：
:In the rat, in response to blastocyst implantation, stromal cells of the endometrium proliferate and differentiate into decidual cells, forming the decidua. After reaching its maximum development, the decidua undergoes regression. This phenomenon appears to be due to an active process involving apoptosis. As there is sparse knowledge concerning the mechanisms of induction of decidual cell death, the potential role of cytokines present in the uterine environment during pregnancy, such as tumor necrosis factor (TNF) and interferon-gamma (INF-gamma) was explored in primary cultures of rat decidual cells. The effects of these factors upon cellular viability, nuclear morphologic alterations, expression, and enzymatic activities of the effector caspases-3/7 were evaluated. The results obtained demonstrated that in contrast to TNF, which did not induce any alteration, INF-gamma and in association with TNF caused a decrease in cell viability and an increase in the appearance of apoptotic bodies in a time-dependent manner that was augmented in the co-presence of TNF. An increase in caspase-3/7 activities after 12 hr of TNF/INF-gamma treatment was also observed. These findings suggest that INF-gamma expressed in the uterine environment may play an important role in regulating apoptosis through potential synergistic mechanisms with TNF and thereby modulate decidual stability and regression during pregnancy.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Almeida A,Correia-da-Silva G,Cepa M,Bell SC,Teixeira NAdoi
10.1002/mrd.20524subject
Has Abstractpub_date
2007-03-01 00:00:00pages
371-7issue
3eissn
1040-452Xissn
1098-2795journal_volume
74pub_type
杂志文章abstract：:Previous studies have shown that in adult male mice, expression of the meg1 gene is restricted to meiotic and early postmeiotic testicular germ cells. We have now analyzed the expression of meg1 during postnatal testicular development and the comparable meiotic stages in the female. The 0.75 kb transcript for meg1 beg...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080380104
更新日期：1994-05-01 00:00:00
abstract：:The mRNAs of the nuclear encoded genes, ornithine decarboxylase (ODCase) and poly(ADP)ribose polymerase (PADPRP), and the mitochondrial encoded genes, cytochrome oxidase I and II (COI and COII) and ATPase 6, are differentially expressed during spermatogenesis (Alcivar et al., 1989: Biol Reprod 41:1133; 1989: Dev Biol ...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2795(199603)43:3<283::AID-MRD1>
更新日期：1996-03-01 00:00:00
abstract：:Estrogen receptor (ER)- (alpha, beta, and both alpha and beta) and aromatase (Ar) knockout (KO) mice have been created to assess the biological effects of estrogens. This review article discusses the ovarian and uterine phenotypes of these mice. The data obtained have confirmed some older inferences about how the ster...
journal_title：Molecular reproduction and development
pub_type： 杂志文章,评审
doi：10.1002/mrd.1039
更新日期：2001-07-01 00:00:00
abstract：:Using alpha and beta spectrin mammalian antibodies on Western blotting, we demonstrated that lizard ovarian follicles contain two isoforms of alpha spectrin, Mr 94 and 134 kDa, and a 230 kDa beta spectrin, and that their pattern modifies in relation to pyriform cell differentiation. In fact, a positive immunoreaction ...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20008
更新日期：2004-01-01 00:00:00
abstract：:During the molecular cloning of the ovine testicular follicle-stimulating (FSH) receptor that couples to the Gs-type effector systems, we discovered novel cDNA clones that were highly homologous. Some of these clones contained an insert of 1,584 bp, which consisted of a divergent 3' region spliced with a 5' region tha...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2795(199712)48:4<458::AID-MRD6>
更新日期：1997-12-01 00:00:00
abstract：:Irregular uterine bleeding is a major side effect of long-acting progestogen-only contraceptives in women, and is the primary reason women discontinue their use. In this study, a mouse model of endometrial breakdown was established using a subcutaneous progesterone implant to understand how irregular bleeding begins. ...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.22686
更新日期：2016-09-01 00:00:00
abstract：:A full-length cDNA encoding the bovine transforming growth factor beta (TGF-beta) receptor type I (bT beta R-I) was isolated from a placenta cDNA library. The deduced protein sequence of 499 residues contains a single transmembrane domain, a cysteinerich extracellular domain, and an intracellular kinase domain with pr...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2795(199801)49:1<1::AID-MRD1>3.
更新日期：1998-01-01 00:00:00
abstract：:Development of female gonads in the chicken is asymmetric. This asymmetry affects gene expression, morphology, and germ cell development; consequently only the left ovary develops into a functional organ, whereas the right ovary remains vestigial. In males, on the other hand, both gonads develop into functional testes...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.22516
更新日期：2015-10-01 00:00:00
abstract：:Although placental transfer of maternal calcium (Ca(2+)) is a crucial process for fetal development, the biochemical mechanisms are not completely elucidated. Especially, mechanisms of syncytiotrophoblast Ca(2+) extrusion into fetal circulation remain to be established. In the current study we have investigated the ch...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.10303
更新日期：2003-07-01 00:00:00
abstract：:This study was designed to examine the recovery of mitochondrial function and endogenous antioxidant systems in vitrified oocytes during extended incubations. After 16 hr of in vitro maturation, bovine meiosis-II oocytes were vitrified, and then surviving oocytes were cultured an additional 8 hr. ATP content, ATP synt...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.21389
更新日期：2011-12-01 00:00:00
abstract：:The objective of this study was to use atomic force microscopy (AFM), with submicron resolution, for morphophologic and morphometric analyses of acrosome intact and acrosome-reacted human sperm heads. A mixed population of acrosome intact and reacted sperm was produced by treating capacitated sperm with A23187, which ...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.10195
更新日期：2002-12-01 00:00:00
abstract：:Diandric and digynic triploid mouse embryos were isolated in the morning on day 10 of gestation. The embryos were separated from their extraembryonic membranes, and the latter were analysed cytogenetically by G-banding to establish the ploidy and sex chromosome constitution of these embryos. The diandric triploid embr...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080250409
更新日期：1990-04-01 00:00:00
abstract：:While most cyclin-dependent kinases (CDKs) are involved in cell cycle control, CDK5 is mostly known for crucial functions in neurogenesis. However, we cloned sea urchin CDK5 from a two-cell stage cDNA library and found that the protein is present in eggs and embryos, up to the pluteus stage, but without associated kin...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.21165
更新日期：2010-05-01 00:00:00
abstract：:One of the hallmarks of mammalian sperm capacitation is the loss of cholesterol from the plasma membrane. Cholesterol has been associated with the formation of detergent insoluble membrane microdomains in many cell types, and sperm from several mammalian species have been shown to contain detergent-resistant membranes...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20540
更新日期：2006-12-01 00:00:00
abstract：:p53, p21, and Rb are proteins with an important role in cell-cycle control. The expression and distribution of these gene products and the apoptotic rate were studied in the marbled-newt testis along the annual cycle to know the role of these factors in the control of spermatogenesis and glandular tissue formation. Th...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.10167
更新日期：2002-10-01 00:00:00
abstract：:Sperm from the decapod crustacean Rhynchocinetes typus undergo dramatic shape changes as they pass from the vas deferens to seawater and interact with the oocyte envelopes. Using FITC-phalloidin and antitubulin antibodies, we were able to localize microfilaments and a tubulin-like protein in R. typus spermatozoon. Mic...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080280410
更新日期：1991-04-01 00:00:00
abstract：:We aimed in this study to explore if sphingosine-1-phosphate (S1P) reduces apoptosis of primordial follicles during cryopreservation of human ovarian cortical samples. Ovarian cortical tissue fragments obtained from young patients who underwent laparoscopic excision of benign ovarian cysts were used for the experiment...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.23043
更新日期：2018-11-01 00:00:00
abstract：:The present studies were performed to establish the effects of the size and number of artificial holes produced in the zona pellucida (ZP) on hatching and trophoblast outgrowth in vitro. Limited partial zona dissection (PZD) produced small, narrow incisions, and zona drilling with acidic Tyrode's (AT) across a larger ...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080300110
更新日期：1991-09-01 00:00:00
abstract：:Global warming represents a major stressful environmental condition that compromises the reproductive efficiency of animals and humans via a rise of body temperature above its physiological homeothermic point (heat stress [HS]). The injuries caused by HS on reproductive function involves both male and female component...
journal_title：Molecular reproduction and development
pub_type： 杂志文章,评审
doi：10.1002/mrd.23123
更新日期：2019-10-01 00:00:00
abstract：:Artificial activation is required for successful intracytoplasmic sperm injection (ICSI) to induce haploidy pronuclear formation with extraction of second polar body. The present study showed that an additional treatment with Phorbol 12-myristate 13-acetate (PMA) followed by Ca(2+) ionophore treatment improved the rat...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20182
更新日期：2005-01-01 00:00:00
abstract：:Fertilization in invertebrates results in tyrosine (Tyr) phosphorylation of several egg proteins. However, the involvement of Tyr phosphorylation in mediating mammalian egg activation has not yet been investigated. Using an antibody specific for phosphotyrosine (P-Tyr), immunoblotting, and densitometric analysis, we f...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2795(199802)49:2<176::AID-MRD8>
更新日期：1998-02-01 00:00:00
abstract：:The murine intracisternal A particle (IAP) proviral elements are expressed at low levels in undifferentiated F9 embryonal carcinoma cells but are highly expressed when F9 cells are induced to differentiate into parietal endoderm-like cells. IAP elements are also expressed in parietal endoderm-like PYS-2 cells. We prev...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080410103
更新日期：1995-05-01 00:00:00
abstract：:MicroRNAs (miRNAs) are small noncoding RNAs that orchestrate numerous cellular processes both under normal physiological conditions as well as in diseases. This review summarizes the functional roles and transcriptional regulation of the highly evolutionarily conserved miRNA, microRNA-31 (miR-31). miR-31 is an importa...
journal_title：Molecular reproduction and development
pub_type： 杂志文章,评审
doi：10.1002/mrd.22678
更新日期：2016-08-01 00:00:00
abstract：:Although the epididymal environment promotes the maturation and survival of spermatozoa, not all spermatozoa remain viable during passage through the epididymis. Does the epididymis has a protective mechanism(s) to segregate the viable sperm from defective spermatozoa? Previously, we identified 260/280 kDa oligomers (...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.23438
更新日期：2020-12-01 00:00:00
abstract：:Male infertility or subfertility is frequently associated with disruption of the hypothalamic-pituitary-testis axis events, like secondary hypogonadism. However, little is known how this condition affects the proteomic composition of the epididymal fluid. In the present study, we evaluated the proteomic changes in the...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.23408
更新日期：2020-09-01 00:00:00
abstract：:Inner cell masses (ICM) from in vitro produced day 8 or 9 bovine blastocysts were isolated by immunosurgery and cultured under different conditions in order to establish which of two feeder cell types and culture media were most efficient in supporting attachment and outgrowth of the bovine ICM cells. The efficiency o...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080400408
更新日期：1995-04-01 00:00:00
abstract：:In the cow, cryopreserved semen is inseminated into the uterus, and most of sperm are removed by backflow and phagocytes. Nevertheless, the mechanism responsible for sperm phagocytosis is unclear. Here, we used cultured bovine uterine epithelial cells (BUECs) to investigate the uterine response to sperm and the mechan...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.22955
更新日期：2018-03-01 00:00:00
abstract：:Spermatozoa from the sperm-rich fractions of the semen of 6 beagle dogs were capacitated and the effect of both zona pellucida (ZP) proteins and progesterone on calcium flux and the acrosome reaction measured. Sperm calcium flux was determined using the dual wavelength calcium probe indo-1/AM (6 microM) in a flow cyto...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1114
更新日期：2001-12-01 00:00:00
abstract：:We compared the results of intracytoplasmic sperm injection (ICSI) that leads to full term development of hybrid (B6C3F1 and B6D2F1) and inbred (C57BL/6) mouse embryos. Although fertilization and pre-implantation development of C57BL/6 eggs were similar to those of F1 hybrid eggs, post-implantation development of the ...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1063
更新日期：2001-09-01 00:00:00
abstract：:The present study was conducted to reveal effects of in ovo injection of nonsteroidal aromatase inhibitor (Fadrozole) or estradiol at day 3 of incubation on mRNA levels of P45017alphahydroxylase (P450c17), P450 aromatase (P450arom) and anti-Müllerian hormone (AMH) in the chicken gonads. The mRNA levels in the gonads a...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2795(200001)55:1<20::AID-MRD4>3
更新日期：2000-01-01 00:00:00
