Differential expression of ornithine decarboxylase, poly(ADP)ribose polymerase, and mitochondrial mRNAs following testosterone administration to hypophysectomized rats.

abstract：:Previously, we cloned and sequenced a sperm specific antigen, designated as HSS (EMBL nomenclature human sperm associated antigen 9: hSPAG9) from human testis (Shankar et al.: Biochem Biophys Res Commun 243:561-565, 1998). The present study was conducted to isolate baboon proteomic homologue in order to find out wheth...

journal_title：Molecular reproduction and development

authors： Shankar S,Mohapatra B,Verma S,Selvi R,Jagadish N,Suri A

更新日期：2004-10-01 00:00:00

abstract：:The ter (teratoma, chromosome 18) mutation causes a deficiency of primordial germ cells (PGCs) in ter/ter embryos from the ter congenic mouse strain at 8.0 days post coitum (dpc). In order to analyse the function of the ter gene, here we examined effects of conditioned medium (CM) from 14.5 dpc testicular and ovarian ...

journal_title：Molecular reproduction and development

authors： Takabayashi S,Sasaoka Y,Yamashita M,Tokumoto T,Ishikawa K,Noguchi M

更新日期：2001-11-01 00:00:00

abstract：:The highly conserved Pumilio protein plays crucial roles in fertility of many organisms acting as a repressor of translation, and causing infertility when mutated. Although one of two human Pumilio homologs, PUMILIO2 is expressed mainly in the germ line, its role in mammalian germ cell development has not been reporte...

journal_title：Molecular reproduction and development

authors： Kusz K,Ginter-Matuszewska B,Ziolkowska K,Spik A,Bierla J,Jedrzejczak P,Latos-Bielenska A,Pawelczyk L,Jaruzelska J

更新日期：2007-06-01 00:00:00

abstract：:Derivation and stable maintenance of porcine induced pluripotent stem cells (piPSCs) is challenging. We herein systematically analyzed two piPSC lines, derived by lentiviral transduction and cultured under either leukemia inhibitory factor (LIF) or fibroblast growth factor (FGF) conditions, to shed more light on the u...

journal_title：Molecular reproduction and development

authors： Secher JO,Ceylan A,Mazzoni G,Mashayekhi K,Li T,Muenthaisong S,Nielsen TT,Li D,Li S,Petkov S,Cirera S,Luo Y,Thombs L,Kadarmideen HN,Dinnyes A,Bolund L,Roelen BA,Schmidt M,Callesen H,Hyttel P,Freude KK

更新日期：2017-03-01 00:00:00

abstract：:In this study cytoskeletal antigens common to brushtail possum and tammar wallaby spermatozoa were characterised using a monoclonal antibody (PSA-10). Using indirect immunofluorescence, the PSA-10 antibody detected antigens predominantly associated with the midpiece and principal piece of mature, permeabilised marsupi...

journal_title：Molecular reproduction and development

authors： Harris MS,Rodger JC

更新日期：1998-08-01 00:00:00

abstract：:Decades worth of research have consistently shown the adverse effects of elevated temperatures on reproductive parameters of livestock species. The objective of this study was to evaluate the developmental and apoptotic responses of porcine in vitro fertilized (IVF) and parthenogenetically activated (PA) embryos heat ...

journal_title：Molecular reproduction and development

authors： Isom SC,Prather RS,Rucker EB 3rd

更新日期：2007-05-01 00:00:00

abstract：:The sequence of ionic changes involved in initiation of acrosomal exocytosis in capacitated mouse spermatozoa was investigated. Earlier studies demonstrated that a large influx of Na+ is required for exocytosis, this Na+ apparently being associated with an increase in intracellular pH (pHi) via an Na(+)-H+ exchanger. ...

journal_title：Molecular reproduction and development

authors： Fraser LR

更新日期：1993-11-01 00:00:00

abstract：:Endometriosis is defined as endometrial tissue outside of the uterine cavity. The pathogenesis of this common disease remains poorly understood. However, the implantation and invasion of the viable cells from retrograde menstruation into the peritoneum is a widely accepted theory. To date, the mechanisms by which cell...

journal_title：Molecular reproduction and development

authors： Chen GT,Tai CT,Yeh LS,Yang TC,Tsai HD

更新日期：2002-07-01 00:00:00

abstract：:Pre-pubertal pig oocytes display reduced developmental competence compared with adult oocytes following in vitro maturation (IVM). Exposure to dibutyryl cyclic adenosine monophosphate (dbcAMP) for the first 20 hr IVM improves development of pre-pubertal oocytes, suggesting that their cAMP content may be inadequate. Th...

journal_title：Molecular reproduction and development

authors： Bagg MA,Nottle MB,Grupen CG,Armstrong DT

更新日期：2006-10-01 00:00:00

abstract：:Ejaculated boar spermatozoa exhibit two types of hyperactivation: full and non-full. Full-type hyperactivation is characterized by the asymmetrical bending of the entire middle piece-principal piece and a twisting/figure-eight-like trajectory, and can be induced by simple incubation with CaCl2 after preincubation with...

journal_title：Molecular reproduction and development

authors： Otsuka N,Harayama H

更新日期：2017-11-01 00:00:00

abstract：:A polymorphism in the igf2 gene of chickens was identified using NlaIII (GenBank accession number AF218827). In some embryos, the igf2 alleles were expressed monoallelically from either maternal or paternal alleles. These data demonstrate that genomic imprinting is not confined to mammalian vertebrates and suggest tha...

journal_title：Molecular reproduction and development

authors： Koski LB,Sasaki E,Roberts RD,Gibson J,Etches RJ

更新日期：2000-07-01 00:00:00

abstract：:Various thiol compounds are known to improve cytoplasmic and/or nuclear maturation of oocytes in vitro. The present study examined the effects of two thiol compounds, cysteine (0.1, 0.5, and 1.0 mM) and cysteamine (50, 100, and 200 microM), on cytoplasmic and nuclear maturation of canine oocytes. Oocytes collected fro...

journal_title：Molecular reproduction and development

authors： Hossein MS,Kim MK,Jang G,Oh HJ,Koo O,Kim JJ,Kang SK,Lee BC,Hwang WS

更新日期：2007-09-01 00:00:00

abstract：:Recent studies have independently shown that cyclic adenosine 3'5'-monophosphate (cAMP) modulation prior to in vitro maturation (IVM) and epidermal growth factor (EGF)-like peptide supplementation during IVM improve subsequent oocyte developmental outcomes. This study investigated the effects of an IVM system that inc...

journal_title：Molecular reproduction and development

authors： Richani D,Wang X,Zeng HT,Smitz J,Thompson JG,Gilchrist RB

更新日期：2014-05-01 00:00:00

abstract：:Mouse oocyte development in vitro has been studied in the past several years, but no evidence showed that the fertilizable oocytes could be obtained from the fetal mouse germ cells before the formation of the primordial follicles. In this study, an efficient and simple method has been established to obtain the mature ...

journal_title：Molecular reproduction and development

authors： Shen W,Li L,Zhang D,Pan Q,Ding M,Deng H

更新日期：2006-10-01 00:00:00

abstract：:During early placental development, tumor suppressors and oncogenes work synergistically to regulate cell proliferation and differentiation in a restrained manner compared with the uncontrollable growth in cancer. One example of this partnership is the regulation of the oncofetal protein HMGA2 by BRCA1. BRCA1 forms a ...

journal_title：Molecular reproduction and development

authors： West RC,Russ JE,Bouma GJ,Winger QA

更新日期：2019-11-01 00:00:00

abstract：:Dead-end1 (Dnd1) expression is restricted to the vertebrate germline where it is believed to activate translation of messenger RNAs (mRNAs) required to protect and promote that unique lineage. Nanos1 is one such germline mRNA whose translation is blocked by a secondary mRNA structure within the open reading frame (ORF...

journal_title：Molecular reproduction and development

authors： Aguero T,Jin Z,Owens D,Malhotra A,Newman K,Yang J,King ML

更新日期：2018-12-01 00:00:00

abstract：:We have previously shown that each of the metalloendoprotease (MEP) inhibitors phosphoramidon, diethylenetriaminepentaacetic acid, and carbobenzoxy-L-phenylalanine, when present only during the human sperm acrosome reaction (AR), will not inhibit the AR or sperm motility but will decrease the number of sperm that pene...

journal_title：Molecular reproduction and development

authors： Díaz-Pérez E,Meizel S

更新日期：1992-02-01 00:00:00

abstract：:Protectin (CD59) is a complement regulatory protein which blocks the membrane attack complex during complement activation. CD59 was identified on the human sperm surface by means of H19, an IgG1 anti-protectin mouse monoclonal antibody. Using indirect immunofluorescence, flow cytometry and immunoperoxidase, CD59 was f...

journal_title：Molecular reproduction and development

authors： Fenichel P,Cervoni F,Hofmann P,Deckert M,Emiliozzi C,Hsi BL,Rossi B

更新日期：1994-07-01 00:00:00

abstract：:Rat epididymal glycoprotein DE associates with the dorsal region of the sperm head during sperm maturation, migrates to the equatorial segment (ES) with the acrosome reaction (AR), and is involved in gamete membrane fusion. In the present study we examined the association of DE with the sperm surface and the relations...

journal_title：Molecular reproduction and development

authors： Cohen DJ,Rochwerger L,Ellerman DA,Morgenfeld MM,Busso D,Cuasnicú PS

更新日期：2000-06-01 00:00:00

abstract：:The role of extracellular matrix (ECM) in the differentiation of tissue types was examined in embryos of Strongylocentrotus purpuratus. We have examined the expression of various tissue-specific molecular markers after disrupting the ECM by culturing embryos in the presence of beta-aminoproprionitrile fumarate (BAPN),...

journal_title：Molecular reproduction and development

authors： Benson S,Rawson R,Killian C,Wilt F

更新日期：1991-07-01 00:00:00

abstract：:We report a method for multiplex genotyping of bovine embryo microblade biopsies. We have tested the reliability of the method and the viability of the embryos in vitro and in vivo. Two polymorphic gene markers (GHR F279Y and PRLR S18N) associated with milk production traits and one marker for sex diagnosis (ZFX/ZFY) ...

journal_title：Molecular reproduction and development

authors： Peippo J,Viitala S,Virta J,Räty M,Tammiranta N,Lamminen T,Aro J,Myllymäki H,Vilkki J

更新日期：2007-11-01 00:00:00

abstract：:Mouse egg activation, which includes release from meiotic metaphase II arrest, results from fertilization-induced increase in intracellular calcium concentration ([Ca2+]i). However, during egg activation caused by exposure to the protein synthesis inhibitor, cycloheximide, [Ca2+]i did not change. Although eggs fertili...

journal_title：Molecular reproduction and development

authors： Moses RM,Kline D

更新日期：1995-06-01 00:00:00

abstract：:Proteins synthesized by DDK mice embryos were analyzed by 2D electrophoresis and a new egg-specific polypeptide, D14, was identified. The protein is characterized by its high rate of synthesis and electrophoretic properties (MW 36,500, pl greater than 8). The synthesis of D14 is strictly developmentally regulated: sta...

journal_title：Molecular reproduction and development

authors： Richoux V,Renard JP,Babinet C

更新日期：1991-03-01 00:00:00

abstract：:Prostaglandins could be involved in various aspects of final differentiation of ovarian follicles. Prostaglandins are generated by the cyclooxygenase (cox) pathway. Until now, the expression pattern of isoforms cox-1 and cox-2 of cyclooxygenase in bovine cumulus-oocyte complexes (COCs) was unknown. Using immunodetecti...

journal_title：Molecular reproduction and development

authors： Nuttinck F,Reinaud P,Tricoire H,Vigneron C,Peynot N,Mialot JP,Mermillod P,Charpigny G

更新日期：2002-01-01 00:00:00

abstract：:Estrogen receptor (ER)- (alpha, beta, and both alpha and beta) and aromatase (Ar) knockout (KO) mice have been created to assess the biological effects of estrogens. This review article discusses the ovarian and uterine phenotypes of these mice. The data obtained have confirmed some older inferences about how the ster...

journal_title：Molecular reproduction and development

authors： Rosenfeld CS,Roberts RM,Lubahn DB

更新日期：2001-07-01 00:00:00

abstract：:The expression of both inhibin alpha- and inhibin/activin beta A-subunit mRNA was examined in equine uteroplacental tissues collected during pregnancy (days 90 to 300). Northern blot analysis revealed that 5 transcripts (7.0, 4.1, 3.4, 2.6, 1.5 kb) of beta A-subunit were present, and the most abundantly expressed tran...

journal_title：Molecular reproduction and development

authors： Yamanouchi K,Hirasawa K,Hasegawa T,Ikeda A,Chang KT,Matsuyama S,Nishihara M,Miyazawa K,Sawasaki T,Tojo H,Tachi C,Takahashi M

更新日期：1997-08-01 00:00:00

abstract：:It has been reported that the MRL/MpJ mouse strain shows several unique phenotypes, including rapid wound healing, inherent collagen disease, heat shock-resistant spermatocytes, and metaphase-specific apoptosis (Msa) in the testis. In the present study, we found the genetic mutation associated with Msa by chromosomal ...

journal_title：Molecular reproduction and development

authors： Namiki Y,Endoh D,Kon Y

更新日期：2003-02-01 00:00:00

abstract：:Mitogen-activated protein kinase (MAPK) plays a crucial role in meiotic maturation of mouse oocytes. In order to understand the mechanism by which MAPK regulates meiotic maturation, we examined the effects of the MAPK pathway inhibitor U0126 on microtubule organization, gamma-tubulin and nuclear mitotic apparatus prot...

journal_title：Molecular reproduction and development

authors： Lee SE,Kim JH,Kim NH

更新日期：2007-07-01 00:00:00

abstract：:In studies of somatic cell nuclear transfer (SCNT), the ability of factors within the oocyte to epigenetically reprogram transferred nuclei is essential for embryonic development of the clone to proceed. However, irregular patterns of X-chromosome inactivation, abnormal expression of imprinted genes, and genomic DNA h...

journal_title：Molecular reproduction and development

authors： Golding MC,Williamson GL,Stroud TK,Westhusin ME,Long CR

更新日期：2011-05-01 00:00:00

abstract：:To establish reliable criteria for the evaluation of nuclear donor embryos, we studied the effect of cell number and cell size of in vitro produced day 6 donor morulae on the rate of blastocyst formation following nuclear transfer to in vitro matured oocytes. In experiment 1, donor embryos were divided into three grou...

journal_title：Molecular reproduction and development

authors： Zakhartchenko V,Wolf E,Palma GA,Brem G

更新日期：1995-09-01 00:00:00