Estrogen receptor- and aromatase-deficient mice provide insight into the roles of estrogen within the ovary and uterus.

abstract：:The timing of the transition from maternal to zygotic control of development (MZT) and the initiation of transcription was studied in domestic cat embryos to determine if there is a temporal association between these phenomena and the development block observed in cat embryos fertilized in vitro. Embryos were derived ...

journal_title：Molecular reproduction and development

authors： Hoffert KA,Anderson GB,Wildt DE,Roth TL

更新日期：1997-10-01 00:00:00

abstract：:Topoisomerase II (topo II) is an essential enzyme that alters DNA topology. This activity is important for a variety of chromosome functions including DNA replication, transcription, recombination, and chromosome condensation and segregation. Previously we localized topo II in mouse gametes and preimplantation embryos...

journal_title：Molecular reproduction and development

authors： St Pierre J,Wright DJ,Rowe TC,Wright SJ

更新日期：2002-03-01 00:00:00

abstract：:The red wolf (Canis rufus) is an endangered species with 194 individuals remaining in the wild and in various captive facilities. Breeding efforts at the Graham, WA site (Point Defiance Zoo and Aquarium) have involved artificial insemination with fresh or frozen semen in an effort to increase population and maximize t...

journal_title：Molecular reproduction and development

authors： Koehler JK,Platz CC Jr,Waddell W,Jones MH,Smith R,Behrns S

更新日期：1994-04-01 00:00:00

abstract：:The aim of the present study is to investigate the effect of insulin-like growth factor (IGF-I) on the development of cultured rat pre-antral follicles. Pre-antral follicles with a diameter between 140 and 160 microm are mechanically isolated from ovaries of 10-day-old rats and cultured in groups for 6 days in FSH and...

journal_title：Molecular reproduction and development

authors： Zhao J,Taverne MA,Van Der Weijden GC,Bevers MM,Van Den Hurk R

更新日期：2001-03-01 00:00:00

abstract：:Copulation produces different stimuli in the female reproductive tract in camelids, which lead to ovulation. Expression of β-nerve growth factor (β-NGF) and its specific receptor, tropomyosin receptor kinase A (TrKA), was studied comparing the oviductal microenvironment of mated and nonmated llamas. β-NGF and TrKA wer...

journal_title：Molecular reproduction and development

authors： Sari LM,Zampini R,Argañaraz ME,Ratto MH,Apichela SA

更新日期：2020-11-01 00:00:00

abstract：:In mammals, Sry, Sox9, and M33 act as regulators at a chromatin level and promote the maturation of embryonic gonads into testes. Recently, it was shown that transcriptional regulation by DNA methylation plays crucial roles in gene expression during the differentiation and development of various cell types. To determi...

journal_title：Molecular reproduction and development

authors： Mizukami T,Kanai Y,Fujisawa M,Kanai-Azuma M,Kurohmaru M,Hayashi Y

更新日期：2008-06-01 00:00:00

abstract：:The formation of cell membrane following CSF-1 stimulation of a macrophage cell line is coordinated with cell cycle progression. The majority of membrane phospholipid accumulates during the S phase and results from cell-cycle dependent oscillations in the rates of phosphatidylcholine biosynthesis and degradation. Both...

journal_title：Molecular reproduction and development

authors： Jackowski S,Xu XX,Rock CO

更新日期：1997-01-01 00:00:00

abstract：:Limited understanding of the importance of known pluripotency factors in pig embryonic stem cells (ESC) impedes the establishment and validation of porcine ESC lines. This study evaluated the expression of known mouse ESC and human ESC (hESC) pluripotency markers in in vivo inner cell mass (ICM) and in vitro-cultured ...

journal_title：Molecular reproduction and development

authors： Blomberg LA,Schreier LL,Talbot NC

更新日期：2008-03-01 00:00:00

abstract：:A multicolor procedure employing fluorescence in situ hybridization is described for detecting chromosomal domains and germinal aneuploidy in late-step spermatids in mice using DNA probes specific for repetitive sequences near the centromeres of chromosomes 8 and X. These probes were nick-translated with biotin- or di...

journal_title：Molecular reproduction and development

authors： Wyrobek A,Lowe X,Pinkel D,Bishop J

更新日期：1995-02-01 00:00:00

abstract：:Previously, we cloned and sequenced a sperm specific antigen, designated as HSS (EMBL nomenclature human sperm associated antigen 9: hSPAG9) from human testis (Shankar et al.: Biochem Biophys Res Commun 243:561-565, 1998). The present study was conducted to isolate baboon proteomic homologue in order to find out wheth...

journal_title：Molecular reproduction and development

authors： Shankar S,Mohapatra B,Verma S,Selvi R,Jagadish N,Suri A

更新日期：2004-10-01 00:00:00

abstract：:Tcp-11 is a candidate for a distorter gene within the t-complex on mouse chromosome 17; although t-complex genes appear to affect sperm function, relatively little is known about mechanisms whereby these genes might play a specific physiological role. We present evidence that the protein TCP-11 is found on the surface...

journal_title：Molecular reproduction and development

authors： Fraser LR,Hosseini R,Hanyalogou A,Talmor A,Dudley RK

更新日期：1997-11-01 00:00:00

abstract：:Insulators are located at the boundaries of differentially regulated genes and delimit their interactions by establishing independent chromatin structures. Recently, an insulator sequence has been found in the 5'-flanking region of arylsulfatase (ARS) gene from sea urchin. To investigate functional conservation of thi...

journal_title：Molecular reproduction and development

authors： Takada T,Iida K,Akasaka K,Yasue H,Torii R,Tsujimoto G,Taira M,Kimura H

更新日期：2000-11-01 00:00:00

abstract：:Differential expression of genes leads to variations in the phenotypes of X and Y sperm, although some differentially expressed gene products are shared through intercellular bridges. Genes differentially expressed in bovine X and Y sperm were identified by a combination of suppression subtractive hybridization (SSH),...

journal_title：Molecular reproduction and development

authors： Chen X,Yue Y,He Y,Zhu H,Hao H,Zhao X,Qin T,Wang D

更新日期：2014-10-01 00:00:00

abstract：:A number of cytokines and growth factors are known to modulate proliferation and differentiation of human endometrium. In this study, the expression of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), and VEGF receptors, fms-like tyrosine kinase (Flt1) and kinase insert domain-containi...

journal_title：Molecular reproduction and development

authors： Wei P,Chen XL,Song XX,Han CS,Liu YX

更新日期：2004-08-01 00:00:00

abstract：:Biochemical and immunoelectron microscopic methods have been used to analyze the distribution of actin in boar spermatozoa and its state of aggregation before and after acrosome reaction. F-actin was detected on sperm head and tail by electron microscopy using an improved phalloidin probe: incubation with a fluorescei...

journal_title：Molecular reproduction and development

authors： Castellani-Ceresa L,Brivio MF,Radaelli G

更新日期：1992-09-01 00:00:00

abstract：:MicroRNAs (miRNAs) are small noncoding RNAs that orchestrate numerous cellular processes both under normal physiological conditions as well as in diseases. This review summarizes the functional roles and transcriptional regulation of the highly evolutionarily conserved miRNA, microRNA-31 (miR-31). miR-31 is an importa...

journal_title：Molecular reproduction and development

authors： Stepicheva NA,Song JL

更新日期：2016-08-01 00:00:00

abstract：:It has been reported that the MRL/MpJ mouse strain shows several unique phenotypes, including rapid wound healing, inherent collagen disease, heat shock-resistant spermatocytes, and metaphase-specific apoptosis (Msa) in the testis. In the present study, we found the genetic mutation associated with Msa by chromosomal ...

journal_title：Molecular reproduction and development

authors： Namiki Y,Endoh D,Kon Y

更新日期：2003-02-01 00:00:00

abstract：:We describe the cloning and characterization of a partial cDNA, x121, that represents an RNA, which is localized in the animal hemisphere of Xenopus oocytes. This RNA is also detected in an animal to vegetal gradient during early cleavage stages. The x121 RNA titer decreases from fertilization through the gastrula sta...

journal_title：Molecular reproduction and development

authors： Kloc M,Reddy BA,Miller M,Eastman E,Etkin LD

更新日期：1991-04-01 00:00:00

abstract：:One of the hallmarks of mammalian sperm capacitation is the loss of cholesterol from the plasma membrane. Cholesterol has been associated with the formation of detergent insoluble membrane microdomains in many cell types, and sperm from several mammalian species have been shown to contain detergent-resistant membranes...

journal_title：Molecular reproduction and development

authors： Thaler CD,Thomas M,Ramalie JR

更新日期：2006-12-01 00:00:00

abstract：:The development of outbred mouse (CF1) zygotes in vitro has been studied using medium SOM in which the concentrations of NaCl (85, 105, 125 mM), glutamine (0, 1, 2 mM), and betaine (0, 1, 2 mM) were varied. The effects of the compounds were studied using a 3(3) factorial experimental arrangement. The inhibitory effect...

journal_title：Molecular reproduction and development

authors： Biggers JD,Lawitts JA,Lechene CP

更新日期：1993-04-01 00:00:00

abstract：:Production of embryos in vitro has enormous potential for research and commercial applications. Unfortunately, in vitro production of porcine embryos is extremely inefficient. Despite the characterization of distinct phenotypes, little is known about the molecular mechanisms and altered physiological processes of in v...

journal_title：Molecular reproduction and development

authors： Miles JR,Blomberg LA,Krisher RL,Everts RE,Sonstegard TS,Van Tassell CP,Zuelke KA

更新日期：2008-06-01 00:00:00

abstract：:We have cloned and sequenced cDNAs encoding autoantigen 1 (AA1), a testis-specific protein and the major autoantigen of the guinea pig sperm acrosome. The cDNA predicts a precursor protein of 244 amino acids including a 21 amino acid hydrophobic, secretory signal sequence. The mature polypeptide is predicted to have a...

journal_title：Molecular reproduction and development

authors： Foster JA,Gerton GL

更新日期：1996-06-01 00:00:00

abstract：:P450aromatase (CYP19) is an essential part of the enzyme complex catalyzing the conversion of androgens into estrogens, which is a key reaction in the sex differentiation in vertebrates. Two full-length cDNAs encoding the genetic distinct CYP19A and CYP19B isozymes were isolated from Atlantic halibut (Hippoglossus hip...

journal_title：Molecular reproduction and development

authors： van Nes S,Moe M,Andersen Ø

更新日期：2005-12-01 00:00:00

abstract：:The mouse metallothionein (MT) gene family consists of four known members (MT-I through IV) clustered on chromosome 8. Studies reported herein examine the expression and regulation of the MT-III and MT-IV genes in specific cell types in the maternal reproductive tract, developing embryo, and fetus known to express the...

journal_title：Molecular reproduction and development

authors： Liang L,Fu K,Lee DK,Sobieski RJ,Dalton T,Andrews GK

更新日期：1996-01-01 00:00:00

abstract：:The present study has used methoxyacetic acid (MAA)-induced depletion of specific germ cell types in the rat and in situ hybridization with nonradioactive riboprobes to determine the stages of the spermatogenic cycle at which there is expression of the mRNA for the basic chromosomal protein transition protein 2 (TP2)....

journal_title：Molecular reproduction and development

authors： Saunders PT,Millar MR,Maguire SM,Sharpe RM

更新日期：1992-12-01 00:00:00

abstract：:The distribution of microtubules was studied during fertilization of the rabbit oocyte by immunofluorescence microscopy after staining with an anti-alpha-tubulin antibody. In ovulated oocytes, microtubules were found exclusively in the meiotic spindle. At fertilization, the paternal centrosome generated sperm astral m...

journal_title：Molecular reproduction and development

authors： Yllera-Fernández MM,Crozet N,Ahmed-Ali M

更新日期：1992-07-01 00:00:00

abstract：:Using a specific protein binding assay we have shown that a spermatozoon of the ascidian Ciona intestinalis contains 1.58 +/- 0.74 x 10(-19) moles of inositol 1,4,5-tri-phosphate (InsP3), while a human spermatozoon contains 6.4 +/- 0.14 x 10(-19) moles. Induction of the acrosome reaction (AR) in both species, by expos...

journal_title：Molecular reproduction and development

authors： Tosti E,Palumbo A,Dale B

更新日期：1993-05-01 00:00:00

abstract：:Global warming represents a major stressful environmental condition that compromises the reproductive efficiency of animals and humans via a rise of body temperature above its physiological homeothermic point (heat stress [HS]). The injuries caused by HS on reproductive function involves both male and female component...

journal_title：Molecular reproduction and development

authors： Boni R

更新日期：2019-10-01 00:00:00

abstract：:An early step in mammalian fertilization is species-restricted binding of sperm to the egg's zona pellucida (ZP), a thick extracellular coat that surrounds eggs. Sperm bind to the ZP of unfertilized eggs, but not to the ZP of fertilized eggs. Shortly after binding to the unfertilized egg ZP, sperm undergo the acrosome...

journal_title：Molecular reproduction and development

authors： Litscher ES,Williams Z,Wassarman PM

更新日期：2009-10-01 00:00:00

abstract：:The normal proto-oncogene c-fms encodes the macrophage growth factor (M-CSF) receptor involved in growth, survival, and differentiation along the monocyte-macrophage lineage of hematopoietic cell development. A major portion of our research concerns unraveling the temporal, molecular, and structural features that dete...

journal_title：Molecular reproduction and development

authors： Rohrschneider LR,Bourette RP,Lioubin MN,Algate PA,Myles GM,Carlberg K

更新日期：1997-01-01 00:00:00