Abstract:
:A novel gene Ggnbp1 was identified during yeast two-hybrid screening of gametogenetin protein 1 (GGN1)-interacting proteins. Ggnbp1 gene was found in mouse, rat, and human genomes but not in sequenced yeast, worms, fly, or fish genomes. Northern blotting analysis revealed that the gene was specifically expressed in the testis but not expressed in the other tissues. In situ hybridization showed that it was testicular germ cell-specific and was specifically expressed in later primary spermatocytes, meiotic cells, and early round spermatids. Western blotting analysis detected a protein of expected size in and only in the testis. By making membrane and cytosolic fractions of germ cells, we were able to show that GGNBP1 associated with the membrane. The identification and characterization of a novel germ cell-specific gene Ggnbp1 is the first step toward the defining of the functions of Ggnbp1 in spermatogenesis.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Zhou Y,Zhao Q,Bishop CE,Huang P,Lu Bdoi
10.1002/mrd.20214keywords:
subject
Has Abstractpub_date
2005-03-01 00:00:00pages
301-7issue
3eissn
1040-452Xissn
1098-2795journal_volume
70pub_type
杂志文章abstract::For mammalian organism, fertilization begins with species-specific recognition between sperm and egg, a process depending upon egg zona pellucida glycoproteins and putative sperm interacting protein(s). In mouse, zona pellucida glycoprotein ZP3 is believed to be the primary receptor for sperm and inducer of sperm acro...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
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journal_title:Molecular reproduction and development
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
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journal_title:Molecular reproduction and development
pub_type: 杂志文章,评审
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Molecular reproduction and development
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
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journal_title:Molecular reproduction and development
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199605)44:1<121::AID-MRD14
更新日期:1996-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1002/mrd.20002
更新日期:2004-04-01 00:00:00
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journal_title:Molecular reproduction and development
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pub_type: 杂志文章
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更新日期:2002-01-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
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更新日期:2005-01-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20854
更新日期:2008-07-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080310206
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2008-05-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199606)44:2<141::AID-MRD1>
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更新日期:2002-04-01 00:00:00