Sperm storage in the female reproductive tract of Scotophilus heathii: role of androgen.

abstract：:Glycogen synthase kinase-3 (GSK-3) is a highly conserved serine/threonine protein kinase implicated in diverse cellular processes. Activity of GSK-3 is essential for meiotic chromatin segregation in oocytes, yet expression and/or function of GSK-3 have not been reported in mammalian preimplantation embryos. Objectives...

journal_title：Molecular reproduction and development

authors： Acevedo N,Wang X,Dunn RL,Smith GD

更新日期：2007-02-01 00:00:00

abstract：:Production of transgenic cattle by microinjection of DNA has been difficult and costly. To explore an alternative method, one- to four-cell bovine embryos were exposed to a replication-defective retrovirus by microinjection of retrovirus producer cells into the perivitelline space. Embryos were cultured in vitro for 3...

journal_title：Molecular reproduction and development

authors： Haskell RE,Bowen RA

更新日期：1995-03-01 00:00:00

abstract：:Spermiogenesis pursues three major objectives: (1) The safeguard of the male genome within the confines of a compact nucleus. (2) The accumulation of enzymes in the acrosome of be released at fertilization. (3) The development of a sperm propelling tail consisting of an axoneme surrounded by a scaffold of keratin-cont...

journal_title：Molecular reproduction and development

authors： Kierszenbaum AL

更新日期：2001-08-01 00:00:00

abstract：:Communication between the oocyte and its somatic cells has been shown to be important in oocyte development. Here we examined how the oocyte may be involved in bovine cumulus cell expansion. Intact bovine cumulus oocyte complexes (COC) were obtained by puncturing antral follicles. From the intact COC, oocytectomised c...

journal_title：Molecular reproduction and development

authors： Ralph JH,Telfer EE,Wilmut I

更新日期：1995-10-01 00:00:00

abstract：:The cytoplasmic beta-actin promoter, commonly used as strong promoter in many gene regulation studies, produces a pattern of male germ cell and preimplantation, embryonic gene expression in transgenic mice. In seven of ten expressing transgenic lines, a chicken beta-actin-lacZ fusion gene was expressed in adult testes...

journal_title：Molecular reproduction and development

authors： Sands AT,Hansen TN,Demayo FJ,Stanley LA,Xin L,Schwartz RJ

更新日期：1993-02-01 00:00:00

abstract：:Transcription factors inhibit or assist RNA polymerases in the initiation and maintenance of transcription; however, the cell specific expression and roles of transcription factors within bovine ovarian follicles during development are unknown. The aim of present study was to determine if the expression of transcripti...

journal_title：Molecular reproduction and development

authors： Zielak AE,Canty MJ,Forde N,Coussens PM,Smith GW,Lonergan P,Ireland JJ,Evans AC

更新日期：2008-05-01 00:00:00

abstract：:The effect of reactive oxygen species production on the motility parameters, DNA integrity, acid phosphatase activity, and protein tyrosine phosphorylation in spermatozoa of the common carp (Cyprinus carpio) was investigated. Spermatozoa were exposed to different concentrations of xanthine and xanthine oxidase (X-XO) ...

journal_title：Molecular reproduction and development

authors： Gazo I,Shaliutina-Kolešová A,Dietrich MA,Linhartová P,Shaliutina O,Cosson J

更新日期：2015-01-01 00:00:00

abstract：:The process of chromatin condensation during spermiogenesis in Rana tigerina is similar to the heterochromatization in somatic cells, where 30 nm fibers are coalesced together into a dense mass in spermatozoa without changing their initial size and nucleosomal organization. This conclusion was supported by the finding...

journal_title：Molecular reproduction and development

authors： Manochantr S,Sretarugsa P,Chavadej J,Sobhon P

更新日期：2005-02-01 00:00:00

abstract：:The objective of this study was to evaluate the effect of progesterone (P4), estradiol (E2), and cortisol (CO) at intraoviductal concentrations on bovine embryo development and quality in vitro. After fertilization of in vitro matured oocytes, zygotes were cultured for 8 days in synthetic oviductal fluid, supplemented...

journal_title：Molecular reproduction and development

authors： Banliat C,Dubuisson F,Corbin E,Beurois J,Tomas D,Le Bourhis D,Salvetti P,Labas V,Mermillod P,Saint-Dizier M

更新日期：2019-06-01 00:00:00

abstract：:Hoxa10, a homeodomain transcription factor, is dynamically expressed in adult uterine endometrium where it is necessary for embryo implantation. Endometrial Hoxa10 expression is driven by estrogen and progesterone. High levels of endometrial Hoxa10 expression coincide with high progesterone levels and development of e...

journal_title：Molecular reproduction and development

authors： Daftary GS,Taylor HS

更新日期：2004-01-01 00:00:00

abstract：:Several proteins with phosphorylated tyrosine residues have been shown to be closely involved in the control meiotic nuclear division. We identified a 42-kD protein in pig oocytes, using a polyclonal antibody to a synthetic phosphotyrosine construct that increases significantly in amount after 12 hr of maturation cult...

journal_title：Molecular reproduction and development

authors： Miyano T,Moor RM,Wooding FB,Shiroo M

更新日期：1996-07-01 00:00:00

abstract：:The homeobox-containing gene family plays a pivotal role in regulating, patterning, and axial morphogenesis in the developing embryo. But there is still very little known about the expression and function of these genes in mammalian oocytes and preimplantation stage embryos. In this study we have used degenerate prime...

journal_title：Molecular reproduction and development

authors： Ponsuksili S,Wimmers K,Adjaye J,Schellander K

更新日期：2001-11-01 00:00:00

abstract：:We have developed a novel reverse transcription-polymerase chain reaction (RT-PCR)-based approach for systematically quantifying in a single experiment the abundances of many different mRNAs in preimplantation mouse embryos. With this approach, the entire mRNA population from a small number of embryos is amplified whi...

journal_title：Molecular reproduction and development

authors： Rambhatla L,Patel B,Dhanasekaran N,Latham KE

更新日期：1995-07-01 00:00:00

abstract：:Amino acid analyses of nuclear basic proteins of an anuran amphibian, Rana catesbeiana, revealed that they are comprised of a full set of core histones and three types of lysine-rich, sperm-specific proteins. On the basis of their amino-acid compositions and partial amino-acid sequences of their trypsin-resistant core...

journal_title：Molecular reproduction and development

authors： Itoh T,Ausio J,Katagiri C

更新日期：1997-06-01 00:00:00

abstract：:The effect of two glycoproteins (estrus-associated glycoprotein [EGP] and tissue inhibitor of metalloproteinase [TIMP-1]) secreted by bovine oviduct cells on in vitro bovine embryo development was assessed. A first set of experiments was conducted to determine whether the embryotrophic activity of the bovine oviduct-c...

journal_title：Molecular reproduction and development

authors： Vansteenbrugge A,Van Langendonckt A,Massip A,Dessy F

更新日期：1997-04-01 00:00:00

abstract：:Triosephosphate isomerase 1 (TPI1) is a member of the glycolytic pathway, which is a critical source of energy for motility in mouse sperm. By immunoblotting, we detected two male, germ line-specific TPI1 bands (Mr 33,400 and 30,800) as well as the somatic-type band (Mr 27,700). Although all three bands were observed ...

journal_title：Molecular reproduction and development

authors： Ijiri TW,Vadnais ML,Lin AM,Huang AP,Cao W,Merdiushev T,Gerton GL

更新日期：2013-10-01 00:00:00

abstract：:The highly conserved Pumilio protein plays crucial roles in fertility of many organisms acting as a repressor of translation, and causing infertility when mutated. Although one of two human Pumilio homologs, PUMILIO2 is expressed mainly in the germ line, its role in mammalian germ cell development has not been reporte...

journal_title：Molecular reproduction and development

authors： Kusz K,Ginter-Matuszewska B,Ziolkowska K,Spik A,Bierla J,Jedrzejczak P,Latos-Bielenska A,Pawelczyk L,Jaruzelska J

更新日期：2007-06-01 00:00:00

abstract：:Estrogen receptor mRNA expression was detected in the head and tail parts of the human epididymis by means of reverse transcription polymerase chain reaction (RT-PCR). Immunocytochemical labelling showed that ciliate and nonciliate cells from the epithelium of the efferent ductules possessed strong to moderate nuclear...

journal_title：Molecular reproduction and development

authors： Ergün S,Ungefroren H,Holstein AF,Davidoff MS

更新日期：1997-08-01 00:00:00

abstract：:Estrogen plays an important role in the human reproductive system and its action is mediated mainly by two specific receptors: α (ERα) and β (ERβ). There are polymorphic variants in both ER genes, and studies showed their association with reproductive outcomes. We aimed to determine the distribution of ERα and ERβ gen...

journal_title：Molecular reproduction and development

authors： Bianco B,Peluso C,Gava MM,Ghirelli-Filho M,Lipay MV,Lipay MA,Christofolini DM,Barbosa CP

更新日期：2011-09-01 00:00:00

abstract：:The present study was conducted to reveal effects of in ovo injection of nonsteroidal aromatase inhibitor (Fadrozole) or estradiol at day 3 of incubation on mRNA levels of P45017alphahydroxylase (P450c17), P450 aromatase (P450arom) and anti-Müllerian hormone (AMH) in the chicken gonads. The mRNA levels in the gonads a...

journal_title：Molecular reproduction and development

authors： Nishikimi H,Kansaku N,Saito N,Usami M,Ohno Y,Shimada K

更新日期：2000-01-01 00:00:00

abstract：:Under the influence of gonadotropins or growth factors, a close cooperation develops between cumulus cells and the oocyte that is implicated in transmitting signals involved in maintaining or releasing the meiotic arrest in the oocyte. While cyclic adenosine 5'-monophosphate (cAMP) is a key molecule in maintaining the...

journal_title：Molecular reproduction and development

authors： Silvestre F,Boni R,Fissore RA,Tosti E

更新日期：2011-10-01 00:00:00

abstract：:Studies of ovine interferon-tau (oIFNtau) gene regulation, an anti-luteolytic factor produced by conceptuses of the ruminant ungulates, have been carried out, but a definitive mechanism for its spatial-temporal transcription has not been elucidated. Recently, specific binding regions for transcription factors AP-1 and...

journal_title：Molecular reproduction and development

authors： Xu N,Takahashi Y,Matsuda F,Sakai S,Christenson RK,Imakawa K

更新日期：2003-05-01 00:00:00

abstract：:Ran, a Ras-related GTPase, is required for transporting proteins in and out of the nucleus during interphase and for regulating the assembly of microtubules. cDNA cloning shows that rat testis, like mouse testis, expresses both somatic and testis-specific forms of Ran-GTPase. The presence of a homologous testis-specif...

journal_title：Molecular reproduction and development

authors： Kierszenbaum AL,Gil M,Rivkin E,Tres LL

更新日期：2002-09-01 00:00:00

abstract：:Abnormal oocyte spindle due to the improper function of ooplasm is associated with female infertility of advanced maternal age. A possible way to overcome this problem is to transfer an oocyte germinal vesicle (GV) which contains genetic materials of a patient with a history of poor embryo development to the cytoplast...

journal_title：Molecular reproduction and development

authors： Li GP,Chen DY,Lian L,Sun QY,Wang MK,Liu JL,Li JS,Han ZM

更新日期：2001-02-01 00:00:00

abstract：:Bicarbonate/CO2 is believed to be the key in vitro effector of sperm capacitation, a process which induces major changes in the sperm plasma membrane in preparation for fertilization. In a flow cytometric study, we examined the effect of bicarbonate on boar spermatozoa using merocyanine, an impermeant lipophilic probe...

journal_title：Molecular reproduction and development

authors： Harrison RA,Ashworth PJ,Miller NG

更新日期：1996-11-01 00:00:00

abstract：:Rainbow trout (Oncorhynchus mykiss) oocytes were incubated for 3 hr in ovarian fluid alone (CC), or cortisol-enriched ovarian fluid [100 or 1,000 ng ml(-1) (CL and CH, respectively)], after which they were fertilized; the growth and development of the embryos reared from these oocytes was monitored until first feed, a...

journal_title：Molecular reproduction and development

authors： Li M,Bureau DP,King WA,Leatherland JF

更新日期：2010-10-01 00:00:00

abstract：:Limited understanding of the importance of known pluripotency factors in pig embryonic stem cells (ESC) impedes the establishment and validation of porcine ESC lines. This study evaluated the expression of known mouse ESC and human ESC (hESC) pluripotency markers in in vivo inner cell mass (ICM) and in vitro-cultured ...

journal_title：Molecular reproduction and development

authors： Blomberg LA,Schreier LL,Talbot NC

更新日期：2008-03-01 00:00:00

abstract：:Ovulated rat oocytes undergo spontaneous activation during in vitro culture. After extrusion of the second polar body, they do not enter interphase but are arrested again in next metaphase-like stage (M III arrest). The present study demonstrates that puromycin and chloral hydrate can trigger transition to interphase ...

journal_title：Molecular reproduction and development

authors： Zernicka-Goetz M

更新日期：1991-02-01 00:00:00

abstract：:PLAC1 is a recently described, trophoblast-specific gene that localizes to a region of the X-chromosome important in placental development. Immunohistochemical analysis demonstrated that PLAC1 polypeptide localizes to the differentiated syncytiotrophoblast throughout gestation (8-41 weeks) as well as a small populatio...

journal_title：Molecular reproduction and development

authors： Massabbal E,Parveen S,Weisoly DL,Nelson DM,Smith SD,Fant M

更新日期：2005-07-01 00:00:00

abstract：:The reproductive tract environment at conception programs the developmental trajectory of the embryo, sets the course of pregnancy, and impacts offspring phenotype and health. Despite the fundamental importance of this stage of reproduction, the rate-limiting regulatory mechanisms operating locally to control fertilit...

journal_title：Molecular reproduction and development

authors： Robertson SA,Zhang B,Chan H,Sharkey DJ,Barry SC,Fullston T,Schjenken JE

更新日期：2017-09-01 00:00:00