Decreased protein tyrosine phosphorylation and membrane fluidity in spermatozoa from infertile men with varicocele.

abstract：:We determined the incidence of activation, male pronuclear formation, and apposition of pronuclei in porcine oocytes following intracytoplasmic injection of various porcine sperm components and foreign species spermatozoa, such as that of cattle, mouse or human. The porcine oocytes were activated by injection of a spe...

journal_title：Molecular reproduction and development

authors： Kim NH,Jun SH,Do JT,Uhm SJ,Lee HT,Chung KS

更新日期：1999-05-01 00:00:00

abstract：:The cytoplasmic beta-actin promoter, commonly used as strong promoter in many gene regulation studies, produces a pattern of male germ cell and preimplantation, embryonic gene expression in transgenic mice. In seven of ten expressing transgenic lines, a chicken beta-actin-lacZ fusion gene was expressed in adult testes...

journal_title：Molecular reproduction and development

authors： Sands AT,Hansen TN,Demayo FJ,Stanley LA,Xin L,Schwartz RJ

更新日期：1993-02-01 00:00:00

abstract：:Plac1 is a recently identified, X-linked gene whose expression is restricted primarily to cells of the trophoblast lineage. It localizes to a chromosomal locus previously implicated in placental growth. We therefore sought to determine if Plac1 is necessary for placental and embryonic development by examining a mutant...

journal_title：Molecular reproduction and development

authors： Jackman SM,Kong X,Fant ME

更新日期：2012-08-01 00:00:00

abstract：:Seminiferous tubules of the testis and epididymal tubules in adult rodents are enveloped by contractile myoid cells, which move sperm and fluids along the male reproductive tract. Myoid cells in the testis influence Sertoli cells by paracrine signaling, but their role in the epididymis is unknown. Electron microscopy ...

journal_title：Molecular reproduction and development

authors： Oliveira RL,Parent A,Cyr DG,Gregory M,Mandato CA,Smith CE,Hermo L

更新日期：2016-06-01 00:00:00

abstract：:Connexin 43 (Cx43), a gap junction protein expressed in differentiated granulosa cells, is necessary for normal follicular development. Cx43 expression and regulation by epidermal growth factor (EGF) were characterized in immature rabbit granulosa cells. Cx43 mRNA was expressed in the granulosa cells of primary follic...

journal_title：Molecular reproduction and development

authors： Kennedy KL,Floyd AA,Clarkson AM,Lee VH

更新日期：2003-01-01 00:00:00

abstract：:While most cyclin-dependent kinases (CDKs) are involved in cell cycle control, CDK5 is mostly known for crucial functions in neurogenesis. However, we cloned sea urchin CDK5 from a two-cell stage cDNA library and found that the protein is present in eggs and embryos, up to the pluteus stage, but without associated kin...

journal_title：Molecular reproduction and development

authors： Lozano JC,Schatt P,Vergé V,Gobinet J,Villey V,Peaucellier G

更新日期：2010-05-01 00:00:00

abstract：:The text below is a transcript of the keynote address Kenneth Manning gave at the symposium honoring E. E. Just that was held on the campus of Howard University on November 21, 2008. In his talk, Manning reflects on his experiences researching and writing Black Apollo of Science: The Life of Ernest Everett Just, his p...

journal_title：Molecular reproduction and development

authors： Manning KR

更新日期：2009-10-01 00:00:00

abstract：:This study investigated the mechanisms for the regulation of intracellular pH in bovine oocytes and embryos. Na(+)/H(+) antiporter activity for the regulation of intracellular pH in the acid to neutral range was detected in both in vitro matured bovine oocytes and in vitro produced embryos. However, the activity of th...

journal_title：Molecular reproduction and development

authors： Lane M,Bavister BD

更新日期：1999-12-01 00:00:00

abstract：:Fragmentation occurs during early developmental stages of electrically activated oocytes and nuclear transfer (NT) embryos. It might contribute to the low developmental rate of porcine NT embryos. The present study was conducted to investigate whether the addition of sugars such as sorbitol or sucrose suppresses fragm...

journal_title：Molecular reproduction and development

authors： Im GS,Yang BS,Lai L,Liu Z,Hao Y,Prather RS

更新日期：2005-06-01 00:00:00

abstract：:An in vitro superfusion method was used to test sex hormone release from different kinds of ovarian follicle (growing follicles, postovulatory follicles, and atretic follicles) in the lizard Podarcis sicula sicula. Sex hormone output changes with the stage of follicle evolution and sexual cycle. Previtellogenetic foll...

journal_title：Molecular reproduction and development

authors： Ciarcia G,Paolucci M,Di Fiore MM

更新日期：1993-07-01 00:00:00

abstract：:The expression of stem cell factor (SCF) mRNA and protein was examined in the postnatal rat testis. Northern blot analysis of total RNA from 1-4 days postpartum (dpp) testes showed none or barely detectable levels of the approximately 6.5 kb SCF transcript. At 5 dpp, there was a striking elevation in this mRNA, a timi...

journal_title：Molecular reproduction and development

authors： Munsie M,Schlatt S,deKretser DM,Loveland KL

更新日期：1997-05-01 00:00:00

abstract：:Copulation produces different stimuli in the female reproductive tract in camelids, which lead to ovulation. Expression of β-nerve growth factor (β-NGF) and its specific receptor, tropomyosin receptor kinase A (TrKA), was studied comparing the oviductal microenvironment of mated and nonmated llamas. β-NGF and TrKA wer...

journal_title：Molecular reproduction and development

authors： Sari LM,Zampini R,Argañaraz ME,Ratto MH,Apichela SA

更新日期：2020-11-01 00:00:00

abstract：:Ran, a Ras-related GTPase, is required for transporting proteins in and out of the nucleus during interphase and for regulating the assembly of microtubules. cDNA cloning shows that rat testis, like mouse testis, expresses both somatic and testis-specific forms of Ran-GTPase. The presence of a homologous testis-specif...

journal_title：Molecular reproduction and development

authors： Kierszenbaum AL,Gil M,Rivkin E,Tres LL

更新日期：2002-09-01 00:00:00

abstract：:The objective of this study was to examine the effects of colcemid treatment during oocyte in vitro maturation (IVM) and embryonic development after parthenogenetic activation (PA) and somatic-cell nucleus transfer (SCNT) in pigs. Immature oocytes were treated with colcemid from 0 to 22, 38 to 42, or 0 to 22 hr follow...

journal_title：Molecular reproduction and development

authors： Lee J,Park JI,Lee GS,Choi JH,Lee ST,Park CK,Kim DY,Hyun SH,Lee E

更新日期：2015-06-01 00:00:00

abstract：:The mouse metallothionein (MT) gene family consists of four known members (MT-I through IV) clustered on chromosome 8. Studies reported herein examine the expression and regulation of the MT-III and MT-IV genes in specific cell types in the maternal reproductive tract, developing embryo, and fetus known to express the...

journal_title：Molecular reproduction and development

authors： Liang L,Fu K,Lee DK,Sobieski RJ,Dalton T,Andrews GK

更新日期：1996-01-01 00:00:00

abstract：:Homozygous deletion of the Lis1 gene (Lis1(-/-)) in mouse resulted in early embryonic lethality immediately after embryo implantation by an undefined mechanism. We seek to define the nature of this demise. LIS1 (pafah1b1) is a 46 kDa protein with seven tryptophan-aspartate (WD) repeats. It docks with many proteins and...

journal_title：Molecular reproduction and development

authors： Cahana A,Jin XL,Reiner O,Wynshaw-Boris A,O'Neill C

更新日期：2003-10-01 00:00:00

abstract：:For mammalian organism, fertilization begins with species-specific recognition between sperm and egg, a process depending upon egg zona pellucida glycoproteins and putative sperm interacting protein(s). In mouse, zona pellucida glycoprotein ZP3 is believed to be the primary receptor for sperm and inducer of sperm acro...

journal_title：Molecular reproduction and development

authors： Li D,Cao S,Xu C

更新日期：2007-10-01 00:00:00

abstract：:Fibulin-1 is a secreted protein associated with elastic matrix fibres and basement membranes. It plays a role in stabilizing blood vessels and can also regulate cell motility and invasiveness. We studied the regulation of the fibulin-1 gene in the rat and human endometrium, an organ where cyclic tissue remodeling and ...

journal_title：Molecular reproduction and development

authors： Haendler B,Yamanouchi H,Lessey BA,Chwalisz K,Hess-Stumpp H

更新日期：2004-07-01 00:00:00

abstract：:Sperm must undergo capacitation to become fertilization competent. Here we validated that monosialotetrahexosylganglioside (GM1 ) localization patterns, which were assessed in the Cap-Score™ Sperm Function Test, reflect a capacitated state in human sperm. First, we defined patterns representing sperm that do or do not...

journal_title：Molecular reproduction and development

authors： Moody MA,Cardona C,Simpson AJ,Smith TT,Travis AJ,Ostermeier GC

更新日期：2017-05-01 00:00:00

abstract：:The developmental pattern of expression of the Strongylocentrotus purpuratus CyIIIb actin gene was determined by RNA blot hybridizations carried out with a gene-specific probe and total embryonic RNA isolated from various stages of development. The results indicate that the CyIIIb mRNA is not detected in the maternal ...

journal_title：Molecular reproduction and development

authors： Flytzanis CN,Bogosian EA,Niemeyer CC

更新日期：1989-01-01 00:00:00

abstract：:Clusterin is a heterodimeric glycoprotein synthesized and secreted by rat Sertoli cells and epididymal epithelium. The goal of this study was to determine the presence of clusterin in the luminal fluid of the cauda epididymides and its association with the membranes of developing spermatozoa in the presence and absenc...

journal_title：Molecular reproduction and development

authors： Mattmueller DR,Hinton BT

更新日期：1992-05-01 00:00:00

abstract：:In the present study propidium iodide was used as a fluorescent dye to stain DNA of cells of hamster testicular origin and fluorescent intensities were analyzed by flow cytometry. We used hamster testicular cells from the first spermatogenic wave to observe the consecutive appearance of the different types of cells du...

journal_title：Molecular reproduction and development

authors： Golan R,Weissenberg R,Oschry Y,Shochat L,Lewin LM

更新日期：2000-02-01 00:00:00

abstract：:The objective of this study was to evaluate the effect of progesterone (P4), estradiol (E2), and cortisol (CO) at intraoviductal concentrations on bovine embryo development and quality in vitro. After fertilization of in vitro matured oocytes, zygotes were cultured for 8 days in synthetic oviductal fluid, supplemented...

journal_title：Molecular reproduction and development

authors： Banliat C,Dubuisson F,Corbin E,Beurois J,Tomas D,Le Bourhis D,Salvetti P,Labas V,Mermillod P,Saint-Dizier M

更新日期：2019-06-01 00:00:00

abstract：:Mitochondria play an important role in the integration and transmission of cell death signals mediated by the Bcl-2 family proteins. Experiments were conducted to determine whether the anti-apoptotic peptides BH4 domain of Bcl-xL (TAT-BH4) and Bax inhibitor peptide (BIP) suppresses heat stress (HS) injury in oocytes b...

journal_title：Molecular reproduction and development

authors： Soto P,Smith LC

更新日期：2009-07-01 00:00:00

abstract：:The non-neuronal monoamine transporters (OCT1, OCT2, EMT, and PMAT) play a key role in the clearance of monoamines from extracellular compartments. In a previous report we described endometrial distribution and cyclic variation of the vesicular monoamine transporter (VMAT2) mRNA and the neuronal norepinephrine transpo...

journal_title：Molecular reproduction and development

authors： Bottalico B,Noskova V,Pilka R,Larsson I,Domanski H,Casslén B,Hansson SR

更新日期：2007-10-01 00:00:00

abstract：:In this report we show that the first event of activation in the human oocyte, the Fertilization current (FC), is a slow transient outward current of 300 pA, which induces a gradual hyperpolarization of the plasma membrane from -20mV to -60mV, 60-120 min after insemination, followed by a repolarization to -20mV. Activ...

journal_title：Molecular reproduction and development

authors： Gianaroli L,Tosti E,Magli C,Iaccarino M,Ferraretti AP,Dale B

更新日期：1994-06-01 00:00:00

abstract：:The development of outbred mouse (CF1) zygotes in vitro has been studied using medium SOM in which the concentrations of NaCl (85, 105, 125 mM), glutamine (0, 1, 2 mM), and betaine (0, 1, 2 mM) were varied. The effects of the compounds were studied using a 3(3) factorial experimental arrangement. The inhibitory effect...

journal_title：Molecular reproduction and development

authors： Biggers JD,Lawitts JA,Lechene CP

更新日期：1993-04-01 00:00:00

abstract：:Gonadotropin-Releasing Hormones (GnRHs) are decapeptides well known to regulate the reproductive cycle. They are expressed not only in the brain, but also in other tissues including the gonads. It is believed that they may be involved in the endocrine and paracrine regulation of the reproductive cycle. To date, two fo...

journal_title：Molecular reproduction and development

authors： Uzbekova S,Lareyre JJ,Madigou T,Davail B,Jalabert B,Breton B

更新日期：2002-05-01 00:00:00

abstract：:The ability of synthetic arginine-glycine-aspartic acid (RGD)-containing peptides to induce intracellular calcium transients similar to those observed at fertilization by spermatozoa in the bovine has been reported (Campbell et al., 2000: Biol Reprod 62:1702-1709; Sessions et al., 2006. Mol Reprod Dev). These results ...

journal_title：Molecular reproduction and development

authors： White KL,Passipieri M,Bunch TD,Campbell KD,Pate B

更新日期：2007-01-01 00:00:00

abstract：:The completion of spermiogenesis requires condensation of the haploid spermatid genome. This task is accomplished in a gradual and relentless manner by first erasing the nucleosomal organization of chromatin while the DNA is protected by transient nuclear proteins TP1 and TP2. Then, the more permanent protamines come ...

journal_title：Molecular reproduction and development

authors： Kierszenbaum AL

更新日期：2001-04-01 00:00:00