Abstract：
:Dickkopf-like 1 (DkkL1) is related to the Dickkopf gene family, a group of proteins that are characterized as secreted antagonists of Wingless (Wnt) signal transduction proteins. DkkL1 mRNA is found in preimplantation mouse embryos and in developing neural tissue, but in adults it is found primarily in the testes. In an effort to elucidate its function, the distribution of DkkL1 protein in mouse testis and mature sperm was analyzed by immuno-histochemistry and immuno-blotting techniques. DkkL1 first appeared in the developing spermatocytes in seminiferous tubules as early as Stage XII, coincident with the appearance of DkkL1 mRNA. Surprisingly, however, DkkL1 localized to the developing acrosome in spermatocytes and spermatids and to the acrosome in mature sperm. Furthermore, DkkL1 was N-glycosylated in the testis, but it did not appear to be excreted, and the DkkL1 in mature sperm was no longer N-glycosylated, suggesting that additional post-translational modifications occurred during the final stages of spermatogenesis. These results identify a member of the Dickkopf family as a novel acrosomal protein that may be involved in acrosome assembly or function, a unique role for a secreted signaling molecule.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Kohn MJ,Kaneko KJ,DePamphilis MLdoi
10.1002/mrd.20314keywords:
subject
Has Abstractpub_date
2005-08-01 00:00:00pages
516-22issue
4eissn
1040-452Xissn
1098-2795journal_volume
71pub_type
杂志文章abstract：:This report is part of a continuing study in which we employ monoclonal antibodies to membrane domains and internal organelles of rat spermatozoa in order to trace events during maturation, capacitation, fertilization, and early development. In the present study, we have used immunocytochemistry at the light and EM le...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080370304
更新日期：1994-03-01 00:00:00
abstract：:Selection of transgenic embryos prior to embryo transfer is a means to increase the efficiency of transgenic livestock production. Among transgenic reporters, cytoplasmic expression of green fluorescent protein (GFP) has features that make it ideal for transgenic embryo selection. The primary objective of this study w...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
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更新日期：1999-04-01 00:00:00
abstract：:RNA interference (RNAi) is an effective tool for studying gene function in oocytes, but no studies have targeted somatic cells of primary cultured cumulus cell-oocyte complexes (COCs). This is probably due to difficulty in introducing RNAi-inducing molecules, such as a short-hairpin RNA (shRNA) gene, into COCs by comm...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20971
更新日期：2009-06-01 00:00:00
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journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080380104
更新日期：1994-05-01 00:00:00
abstract：:Derivation and stable maintenance of porcine induced pluripotent stem cells (piPSCs) is challenging. We herein systematically analyzed two piPSC lines, derived by lentiviral transduction and cultured under either leukemia inhibitory factor (LIF) or fibroblast growth factor (FGF) conditions, to shed more light on the u...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.22771
更新日期：2017-03-01 00:00:00
abstract：:We report use of an in vitro assay (Barbato et al., 1998: Biol Reprod 58:686-699) to assess binding ability of cauda epididymal mouse sperm to a surrogate zona pellucida and effect of a synthetic peptide (Amann et al., 1999: J Androl 20: 42-46) on fertilization ability in in vitro fertilization (IVF) tests. Sperm from...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/1098-2795(200012)57:4<406::AID-MRD13>3.0.C
更新日期：2000-12-01 00:00:00
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journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.22066
更新日期：2012-09-01 00:00:00
abstract：:The cytoplasmic beta-actin promoter, commonly used as strong promoter in many gene regulation studies, produces a pattern of male germ cell and preimplantation, embryonic gene expression in transgenic mice. In seven of ten expressing transgenic lines, a chicken beta-actin-lacZ fusion gene was expressed in adult testes...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080340202
更新日期：1993-02-01 00:00:00
abstract：:The role of extracellular matrix (ECM) in the differentiation of tissue types was examined in embryos of Strongylocentrotus purpuratus. We have examined the expression of various tissue-specific molecular markers after disrupting the ECM by culturing embryos in the presence of beta-aminoproprionitrile fumarate (BAPN),...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080290303
更新日期：1991-07-01 00:00:00
abstract：:Spermatozoa from the sperm-rich fractions of the semen of 6 beagle dogs were capacitated and the effect of both zona pellucida (ZP) proteins and progesterone on calcium flux and the acrosome reaction measured. Sperm calcium flux was determined using the dual wavelength calcium probe indo-1/AM (6 microM) in a flow cyto...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1114
更新日期：2001-12-01 00:00:00
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journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.10110
更新日期：2002-04-01 00:00:00
abstract：:Previously, we cloned and sequenced a sperm specific antigen, designated as HSS (EMBL nomenclature human sperm associated antigen 9: hSPAG9) from human testis (Shankar et al.: Biochem Biophys Res Commun 243:561-565, 1998). The present study was conducted to isolate baboon proteomic homologue in order to find out wheth...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20164
更新日期：2004-10-01 00:00:00
abstract：:Production of transgenic cattle by microinjection of DNA has been difficult and costly. To explore an alternative method, one- to four-cell bovine embryos were exposed to a replication-defective retrovirus by microinjection of retrovirus producer cells into the perivitelline space. Embryos were cultured in vitro for 3...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080400316
更新日期：1995-03-01 00:00:00
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journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20908
更新日期：2008-11-01 00:00:00
abstract：:The normal proto-oncogene c-fms encodes the macrophage growth factor (M-CSF) receptor involved in growth, survival, and differentiation along the monocyte-macrophage lineage of hematopoietic cell development. A major portion of our research concerns unraveling the temporal, molecular, and structural features that dete...
journal_title：Molecular reproduction and development
pub_type： 杂志文章,评审
doi：10.1002/(SICI)1098-2795(199701)46:1<96::AID-MRD15>
更新日期：1997-01-01 00:00:00
abstract：:Using a specific protein binding assay we have shown that a spermatozoon of the ascidian Ciona intestinalis contains 1.58 +/- 0.74 x 10(-19) moles of inositol 1,4,5-tri-phosphate (InsP3), while a human spermatozoon contains 6.4 +/- 0.14 x 10(-19) moles. Induction of the acrosome reaction (AR) in both species, by expos...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080350109
更新日期：1993-05-01 00:00:00
abstract：:The dysregulation of microRNAs (miRNAs) plays an important role in asthenozoospermia. This study evaluated the sperm microRNA-423-5p (miR-423-5p) expression in asthenozoospermia and normozoospermia, exploring the role of miR-423-5p in asthenozoospermia. Eighty participants were divided into asthenozoospermic (AZS, n =...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.23454
更新日期：2021-01-28 00:00:00
abstract：:Connexin 43 (Cx43), a gap junction protein expressed in differentiated granulosa cells, is necessary for normal follicular development. Cx43 expression and regulation by epidermal growth factor (EGF) were characterized in immature rabbit granulosa cells. Cx43 mRNA was expressed in the granulosa cells of primary follic...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.10219
更新日期：2003-01-01 00:00:00
abstract：:Carp hyosophorin (HSP) is purified from oocytes. It is a highly glycosylated protein (10% protein and 90% carbohydrate) of high molecular weight (>100 kDa) and is localized in the cortical granules of oocytes. During cortical reaction carp HSP is exocytosed into the perivitelline space and is rapidly cleaved to the lo...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2795(199903)52:3<288::AID-MRD6>
更新日期：1999-03-01 00:00:00
abstract：:During early placental development, tumor suppressors and oncogenes work synergistically to regulate cell proliferation and differentiation in a restrained manner compared with the uncontrollable growth in cancer. One example of this partnership is the regulation of the oncofetal protein HMGA2 by BRCA1. BRCA1 forms a ...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.23255
更新日期：2019-11-01 00:00:00
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journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20868
更新日期：2008-07-01 00:00:00
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journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2795(199710)48:2<208::AID-MRD8>
更新日期：1997-10-01 00:00:00
abstract：:Human embryonic stem cells (hESCs) hold great promise for cell-based therapies and drug screening applications. However, growing and processing large quantities of undifferentiated hESCs is a challenging task. Conventionally, hESCs are passaged as clusters, which can limit their growth efficiency and use in downstream...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20809
更新日期：2008-05-01 00:00:00
abstract：:Meiotic progression requires the translation of maternal mRNAs in a strict temporal order. In isolated animal oocytes, translation of maternal mRNAs containing a cytoplasmic polyadenylation element (CPE), such as cyclin B, is activated by in vitro stimulation of meiotic resumption which induces phosphorylation of CPEB...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.22599
更新日期：2016-01-01 00:00:00
abstract：:A method for assessment of the human sperm acrosome reaction is reported using fluorescein isothiocyanate (FITC)-conjugated Concanavalin A (ConA). The technique involved labelling prefixed spermatozoa, where only those spermatozoa that showed a complete loss of the acrosome bound FITC-ConA to the acrosomal region. Com...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080250306
更新日期：1990-03-01 00:00:00
abstract：:We have previously reported metabolic cooperation between Sertoli and peritubular myoid cells in terms of synthesis of one of the main testicular extracellular matrix (ECM) constituents, glycosaminoglycans (GAG). This study concerns Sertoli cell ECM-peritubular myoid cell interactions in terms of GAG synthesis. We hav...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080350208
更新日期：1993-06-01 00:00:00
abstract：:The effects of cryopreservation on the frequency and type of chromosomal abnormalities in human sperm were investigated. Employing a technique that enables direct visualization of human sperm chromosomes following in vitro penetration of hamster oocytes, sperm samples from 10 normal men were examined before and after ...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080300213
更新日期：1991-10-01 00:00:00
abstract：:During the molecular cloning of the ovine testicular follicle-stimulating (FSH) receptor that couples to the Gs-type effector systems, we discovered novel cDNA clones that were highly homologous. Some of these clones contained an insert of 1,584 bp, which consisted of a divergent 3' region spliced with a 5' region tha...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2795(199712)48:4<458::AID-MRD6>
更新日期：1997-12-01 00:00:00
abstract：:Activation of bovine oocytes by experimental procedures that closely mimic normal fertilization and allow to obtain haploid oocytes is essential both for intracytoplasmic sperm injection (ICSI) and for nuclear transfer. Therefore, with the goal of producing haploid activated oocytes, this study evaluated whether bohem...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2795(200004)55:4<422::AID-MRD10
更新日期：2000-04-01 00:00:00
abstract：:To study self-renewal, genetic modification, and differentiation of avian spermatogonial stem cells (SSCs), we isolated chicken SSCs from fetal testes on the 16th hatching day via enzyme digestion, and then cultured the SSCs over 2 months after purification in vitro. SSCs were identified by alkaline phosphatase staini...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.21147
更新日期：2010-04-01 00:00:00
