Abstract:
:Three staining procedures to detect sperm acrosome integrity were compared via electron microscopy. Stains were applied to epididymal, freshly ejaculated, in vivo capacitated, and sonicated sperm cells in addition to spermatozoa displaying sequentially removed plasma and outer and inner acrosomal membranes. Sequential membrane removal procedures resulted in removal of plasma membranes from 73% of all sperm cells, removal of plasma and outer acrosomal membranes from 74% of all sperm cells, and removal of plasma and outer and inner acrosomal membranes from 87% of all sperm cells as determined by electron microscopy. Live/dead staining results were not statistically different from subjective microscopic motility evaluations (P less than 0.005) for epididymal, sonicated, freshly ejaculated, and in vivo capacitated sperm samples. All three stains assessed were similarly capable of detecting the acrosome status of freshly ejaculated and of sonicated spermatozoa compared to data obtained by electron microscopy (P = 0.010). However, only the Bryan-Akruk stain afforded data that were closely correlated with data obtained via electron microscopy for all sperm types assessed; the latter included in vivo capacitated spermatozoa and sperm cells rendered free of plasma membranes. Results confirmed an earlier report by successfully effecting sequential removal of rabbit acrosomal membranes and documented use of the Bryan-Akruk acrosomal stain for evaluation of sperm cell populations for fertilizing ability. These findings should prove useful in further investigations of mechanisms involved in achievement of fertilizing ability by rabbit spermatozoa.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
McBride CE,Fayrer-Hosken RA,Srivastava PN,Brackett BGdoi
10.1002/mrd.1080260106subject
Has Abstractpub_date
1990-05-01 00:00:00pages
30-9issue
1eissn
1040-452Xissn
1098-2795journal_volume
26pub_type
杂志文章abstract::Estrogen receptor mRNA expression was detected in the head and tail parts of the human epididymis by means of reverse transcription polymerase chain reaction (RT-PCR). Immunocytochemical labelling showed that ciliate and nonciliate cells from the epithelium of the efferent ductules possessed strong to moderate nuclear...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199708)47:4<448::AID-MRD12
更新日期:1997-08-01 00:00:00
abstract::An early step in mammalian fertilization is species-restricted binding of sperm to the egg's zona pellucida (ZP), a thick extracellular coat that surrounds eggs. Sperm bind to the ZP of unfertilized eggs, but not to the ZP of fertilized eggs. Shortly after binding to the unfertilized egg ZP, sperm undergo the acrosome...
journal_title:Molecular reproduction and development
pub_type: 杂志文章,评审
doi:10.1002/mrd.21046
更新日期:2009-10-01 00:00:00
abstract::In the present study propidium iodide was used as a fluorescent dye to stain DNA of cells of hamster testicular origin and fluorescent intensities were analyzed by flow cytometry. We used hamster testicular cells from the first spermatogenic wave to observe the consecutive appearance of the different types of cells du...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(200002)55:2<205::AID-MRD10
更新日期:2000-02-01 00:00:00
abstract::Prostaglandins could be involved in various aspects of final differentiation of ovarian follicles. Prostaglandins are generated by the cyclooxygenase (cox) pathway. Until now, the expression pattern of isoforms cox-1 and cox-2 of cyclooxygenase in bovine cumulus-oocyte complexes (COCs) was unknown. Using immunodetecti...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1135
更新日期:2002-01-01 00:00:00
abstract::Previously we demonstrated that lines of transgenic mice carrying the herpes simplex type 1 virus thymidine kinase (HSV1-tk) reporter gene are male-sterile. Ectopic transcription of the HSV1-tk reporter in the testis was initiated downstream of the normal translation initiation codon and truncated proteins consistent ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080410405
更新日期:1995-08-01 00:00:00
abstract::A method for assessment of the human sperm acrosome reaction is reported using fluorescein isothiocyanate (FITC)-conjugated Concanavalin A (ConA). The technique involved labelling prefixed spermatozoa, where only those spermatozoa that showed a complete loss of the acrosome bound FITC-ConA to the acrosomal region. Com...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080250306
更新日期:1990-03-01 00:00:00
abstract::In Ciona intestinalis, sperm penetration through the egg vitelline coat is an essential event of fertilization. We investigated whether trypsin- and chymotrypsin-like enzymes are involved in this event. Inhibitors and peptide substrates for chymotrypsin-like enzymes blocked the overall process of fertilization in a co...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080260405
更新日期:1990-08-01 00:00:00
abstract::We have previously shown that bovine oocytes parthenogenetically activated after 40 hours (hr) of in vitro maturation proceed through the cell cycle faster than those after 20 hr of maturation. In the present study, we used this model of different speed of nuclear progression to investigate the correlation of two hall...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.10072
更新日期:2002-05-01 00:00:00
abstract::It is known that the hypothalamic-pituitary-gonadal axis participates in the sex change of hermaphrodite teleosts, and gonadal steroid hormones mediate this physiological process. The secretion of gonadal steroids is directly regulated by signaling pathways involving gonadotropins (GtHs) and gonadotropin receptors (Gt...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.21319
更新日期:2011-06-01 00:00:00
abstract::LIN28B is an RNA-binding protein necessary for maintaining pluripotency in stem cells and plays an important role in trophoblast cell differentiation. LIN28B action on target gene function often involves the Let-7 miRNA family. Previous work in cancer cells revealed that LIN28 through Let-7 miRNA regulates expression ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.23226
更新日期:2019-09-01 00:00:00
abstract::The objective of the present study was to determine whether Fyn kinase participated in signaling events during sperm-egg interactions, sperm incorporation, and meiosis II. The functional requirement of Fyn kinase activity in these events was tested through the use of the protein kinase inhibitor SKI-606 (Bosutinib) an...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.21034
更新日期:2009-09-01 00:00:00
abstract::Under the influence of gonadotropins or growth factors, a close cooperation develops between cumulus cells and the oocyte that is implicated in transmitting signals involved in maintaining or releasing the meiotic arrest in the oocyte. While cyclic adenosine 5'-monophosphate (cAMP) is a key molecule in maintaining the...
journal_title:Molecular reproduction and development
pub_type: 杂志文章,评审
doi:10.1002/mrd.21332
更新日期:2011-10-01 00:00:00
abstract::Spindle positioning is an imperative cellular process that regulates a number of different developmental events throughout embryogenesis. The spindle must be properly positioned in embryos not only for the segregation of chromosomes, but also to segregate developmental determinants into different daughter blastomeres....
journal_title:Molecular reproduction and development
pub_type: 杂志文章,评审
doi:10.1002/mrd.22418
更新日期:2014-10-01 00:00:00
abstract::An in vitro superfusion method was used to test sex hormone release from different kinds of ovarian follicle (growing follicles, postovulatory follicles, and atretic follicles) in the lizard Podarcis sicula sicula. Sex hormone output changes with the stage of follicle evolution and sexual cycle. Previtellogenetic foll...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080350307
更新日期:1993-07-01 00:00:00
abstract::Expression of ovine interferon-tau (oIFNtau), a factor essential for the process of maternal recognition of pregnancy in ruminant ungulates, is restricted to the trophoblast. However, the molecular mechanisms by which oIFNtau expression is restricted to the trophectoderm have not been fully elucidated. The objective o...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20457
更新日期:2006-05-01 00:00:00
abstract::Testis-specific protein kinases are important because of their potential role in spermiogenesis, sperm maturation, and sperm function. In the present study, a novel serine-threonine kinase with high identity to human serine-threonine kinase 31 (STK31) was cloned from equine testis and expression of the protein was cha...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20792
更新日期:2008-05-01 00:00:00
abstract::Development of female gonads in the chicken is asymmetric. This asymmetry affects gene expression, morphology, and germ cell development; consequently only the left ovary develops into a functional organ, whereas the right ovary remains vestigial. In males, on the other hand, both gonads develop into functional testes...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22516
更新日期:2015-10-01 00:00:00
abstract::The developmental pattern of expression of the Strongylocentrotus purpuratus CyIIIb actin gene was determined by RNA blot hybridizations carried out with a gene-specific probe and total embryonic RNA isolated from various stages of development. The results indicate that the CyIIIb mRNA is not detected in the maternal ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080010309
更新日期:1989-01-01 00:00:00
abstract::The homeobox-containing gene family plays a pivotal role in regulating, patterning, and axial morphogenesis in the developing embryo. But there is still very little known about the expression and function of these genes in mammalian oocytes and preimplantation stage embryos. In this study we have used degenerate prime...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1091
更新日期:2001-11-01 00:00:00
abstract::The neomycin-resistant gene (neo(r)) is probably the most commonly used selectable marker gene in gene targeting and gene transfection research. In this study, the neo(r) gene construct was introduced into in vitro cultured goat foetal fibroblast cells (IV-5), and the cells were selected with 900 microg/ml G418. The G...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1143
更新日期:2002-02-01 00:00:00
abstract::The analysis of differences in gene expression, responding to cryopreservation may explain some of the observed differences in further development of the preimplantation stage embryos. The aim of this study was to create a link, for the first time, between morphological/developmental observations and gene activity cha...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20450
更新日期:2006-06-01 00:00:00
abstract::In this review, we discuss the expression, regulation, downstream mechanisms, and function of stress-induced stress enzymes in mammalian oocytes, peri-implantation embryos, and the stem cells derived from those embryos. Recent reports suggest that stress enzymes mediate developmental functions during early mammalian d...
journal_title:Molecular reproduction and development
pub_type: 杂志文章,评审
doi:10.1002/mrd.20787
更新日期:2008-04-01 00:00:00
abstract::The objective of this study was to examine the effects of different culture media used for maturation of bovine oocytes on in vitro embryo development following in vitro fertilization. Oocytes were aspirated from 2-5 mm follicles of ovaries collected at a local abattoir. The oocyte-cumulus complexes (OCCs) were cultur...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080310113
更新日期:1992-01-01 00:00:00
abstract::The formation of cell membrane following CSF-1 stimulation of a macrophage cell line is coordinated with cell cycle progression. The majority of membrane phospholipid accumulates during the S phase and results from cell-cycle dependent oscillations in the rates of phosphatidylcholine biosynthesis and degradation. Both...
journal_title:Molecular reproduction and development
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1098-2795(199701)46:1<24::AID-MRD5>3
更新日期:1997-01-01 00:00:00
abstract::Morphogenesis of the Eledone cirrhosa sperm nucleus, as studied by electron microscopic techniques, is compared with that of Octopus vulgaris. Both species of cephalopods belong to the family Octopodidae. The results indicate that extensive nuclear helicoidization during E. cirrhosa spermiogenesis is brought about by ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.10124
更新日期:2002-07-01 00:00:00
abstract::The aim of this study was to elucidate the relationship between protein tyrosine phosphorylation state and sperm characteristics in frozen-stored spermatozoa of Japanese Black bulls. The spermatozoa were washed with PBS containing polyvinyl alcohol and then incubated with cell-permeable cAMP analog cBiMPS to induce fl...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.21233
更新日期:2010-10-01 00:00:00
abstract::Zona pellucida thickness was measured daily in zygotes and cleavage stage embryos. Measurements were performed on a Nikon inverted microscope equipped with Hoffman modulation optics, using an ocular micrometer. Zona thickness of each zygote/embryo was measured four times, the zygote/embryo was then "rolled over," and ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199705)47:1<99::AID-MRD13>
更新日期:1997-05-01 00:00:00
abstract::Germ cells require communication with associated somatic cells for normal gametogenesis, as exemplified by an oocyte that interacts with granulosa cells via paracrine factors as well as gap junctions located at sites of contact between these two cell types. The objective of the present study was to define the mechanis...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22446
更新日期:2015-02-01 00:00:00
abstract::Maternal immune tolerance to the semi-allogenic fetus is required for successful pregnancy in mammals. Monoclonal nonspecific suppressor factor beta (MNSFB) is an immunosuppressive factor present in uterine epithelial and stromal cells, as well as in macrophages and T cells. Although the functional neutralization of M...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22495
更新日期:2015-06-01 00:00:00
abstract::Clusterin is a heterodimeric glycoprotein synthesized and secreted by rat Sertoli cells and epididymal epithelium. The goal of this study was to determine the presence of clusterin in the luminal fluid of the cauda epididymides and its association with the membranes of developing spermatozoa in the presence and absenc...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080320112
更新日期:1992-05-01 00:00:00