Activation mechanism and function of the MAP kinase cascade.


:MAP kinase (MAPK) and its activator, MAP kinase kinase (MAPKK), are commonly activated by a variety of extracellular stimuli in mammalian cells and in the process of Xenopus oocyte maturation. In order to investigate the function of the MAPK cascade in oocyte maturation, we produced an anti-Xenopus MAPKK which specifically reacts with MAPKK in vitro. When this antibody was microinjected into immature oocytes, MAPK activation induced by progesterone was prevented. Surprisingly, H1 kinase activation and germinal vesicle breakdown were also inhibited in the oocytes injected with this antibody. These results suggest that the MAPK cascade plays an important role in the maturation promoting factor (MPF) activation during the oocyte maturation process. When this antibody together with Mos was microinjected into Xenopus two-cell embryos, the Mos-induced metaphase arrest (CSF arrest) was prevented. Thus, the MAPK cascade may mediate CSF arrest. During Xenopus early embryogenesis, a low but significant level of MAPK remains active. Injection of mRNA encoding a constitutively active MAPKK resulted in mesoderm induction in animal cap explants. In addition, fibroblast growth-factor (FGF)-induced mesoderm induction was inhibited by expressing CL100 (a MAP kinase phosphatase) in animal cap explants. Thus the MAPK cascade may be involved in the mesoderm induction of Xenopus embryos. The activation pathways and roles of the MAPKK/MAPK cascade in various signaling processes will be discussed.


Mol Reprod Dev


Gotoh Y,Nishida E




Has Abstract


1995-12-01 00:00:00












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    abstract::The sperm nucleus has a unique chromatin structure where the DNA is highly condensed and associated with specific proteins, the protamines. It is a nondividing cell which is also transcriptionally inactive. After fusion with an oocyte, the sperm nucleus undergoes decondensation and, in the same time, starts replicatio...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Hazzouri M,Rousseaux S,Mongelard F,Usson Y,Pelletier R,Faure AK,Vourc'h C,Sèle B

    更新日期:2000-03-01 00:00:00

  • Paracrine effects of bFGF and KGF on the process of mouse blastocyst implantation.

    abstract::Implantation is a complex process that requires the interaction of the blastocyst, and subsequently, that of the developing embryos with the endometrium. Several growth factors and cytokines are involved in implantation, but the details of their actions as related to the regulation of blastocyst implantation remain un...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Taniguchi F,Harada T,Yoshida S,Iwabe T,Onohara Y,Tanikawa M,Terakawa N

    更新日期:1998-05-01 00:00:00

  • Sex determination and gender expression: Reproductive investment in snails.

    abstract::Sex determination is generally seen as an issue of importance for separate-sexed organisms; however, when considering other sexual systems, such as hermaphroditism, sex allocation is a less-binary form of sex determination. As illustrated here, with examples from molluscs, this different vantage point can offer import...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章,评审


    authors: Koene JM

    更新日期:2017-02-01 00:00:00

  • Oocyte maturation in Xenopus laevis is blocked by the hormonal herbicide, 2,4-dichlorophenoxy acetic acid.

    abstract::Oocyte maturation is dependent on a complex program of morphological, ultrastructural, and biochemical signaling events, and if disrupted could lead to decreased fertility and population decline. The in vitro sensitivity of amphibian oocytes and oocyte maturation to plant growth factor and widely used hormonal herbici...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Stebbins-Boaz B,Fortner K,Frazier J,Piluso S,Pullen S,Rasar M,Reid W,Sinclair K,Winger E

    更新日期:2004-02-01 00:00:00

  • Ran, a GTP-binding protein involved in nucleocytoplasmic transport and microtubule nucleation, relocates from the manchette to the centrosome region during rat spermiogenesis.

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    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Kierszenbaum AL,Gil M,Rivkin E,Tres LL

    更新日期:2002-09-01 00:00:00

  • Purification and biological characterization of N-acetyl beta-D glucosaminidase from Bufo arenarum spermatozoa.

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    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Martínez ML,Martelotto L,Cabada MO

    更新日期:2000-10-01 00:00:00

  • Timing of MAP kinase inactivation effects on emission of polar body in porcine oocytes activated by Ca2+ ionophore.

    abstract::Artificial activation is required for successful intracytoplasmic sperm injection (ICSI) to induce haploidy pronuclear formation with extraction of second polar body. The present study showed that an additional treatment with Phorbol 12-myristate 13-acetate (PMA) followed by Ca(2+) ionophore treatment improved the rat...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Ito J,Shimada M

    更新日期:2005-01-01 00:00:00

  • Non-hormonal targets underlying endometriosis: A focus on molecular mechanisms.

    abstract::Endometriosis is regarded as a hormone-dependent disease. Current therapeutic approaches to treating this common gynecological disorder mainly depend on surgical and hormonal interventions, but the high rate of disease recurrence as well as the side effects related to such therapies make it difficult for patients to r...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章,评审


    authors: Che XH,Chen YC,Chen CL,Ye XL,Zhu H

    更新日期:2015-06-01 00:00:00

  • Effect of vitrification on promoter methylation and the expression of pluripotency and differentiation genes in mouse blastocysts.

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    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Zhao XM,Du WH,Hao HS,Wang D,Qin T,Liu Y,Zhu HB

    更新日期:2012-07-01 00:00:00

  • Differential growth, cell proliferation, and apoptosis of mouse embryo in various culture media and in coculture.

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    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Xu JS,Chan ST,Lee WW,Lee KF,Yeung WS

    更新日期:2004-05-01 00:00:00

  • High iNOS mRNA and protein localization during late pregnancy suggest a role for nitric oxide in mouse pubic symphysis relaxation.

    abstract::Remodeling and relaxation of the mouse pubic symphysis (PS) are central events in parturition. The mouse PS remodels in a hormone-controlled process that involves the modification of the fibrocartilage into an interpubic ligament (IpL), followed by its relaxation prior to parturition. It is recognized that nitric oxid...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Moro CF,Consonni SR,Rosa RG,Nascimento MA,Joazeiro PP

    更新日期:2012-04-01 00:00:00

  • Decreased protein tyrosine phosphorylation and membrane fluidity in spermatozoa from infertile men with varicocele.

    abstract::Varicocele is a prevalent pathology among infertile men. The mechanisms linking this condition to infertility, however, are poorly understood. Our previous work showed a relationship between sperm functional quality and the ability of spermatozoa to respond to capacitating conditions with increased membrane fluidity a...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Buffone MG,Brugo-Olmedo S,Calamera JC,Verstraeten SV,Urrutia F,Grippo L,Corbetta JP,Doncel GF

    更新日期:2006-12-01 00:00:00

  • Molecular cloning, structure, and expression of a testicular follitropin receptor with selective alteration in the carboxy terminus that affects signaling function.

    abstract::During the molecular cloning of the ovine testicular follicle-stimulating (FSH) receptor that couples to the Gs-type effector systems, we discovered novel cDNA clones that were highly homologous. Some of these clones contained an insert of 1,584 bp, which consisted of a divergent 3' region spliced with a 5' region tha...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Yarney TA,Jiang L,Khan H,MacDonald EA,Laird DW,Sairam MR

    更新日期:1997-12-01 00:00:00

  • Role of the major ecto-phosphoprotein in sperm flagellar motility using a cell electroporation method.

    abstract::Previous studies from our laboratory have identified MPS, a 100-kDa protein, as the major phosphoprotein substrate of caprine sperm ecto-cyclic AMP independent protein kinase. In this study the isolated (32)P-labelled MPS has been incorporated into mature caprine (Capra indicus) cauda-epididymal spermatozoa with the h...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Maiti A,Mishra KP,Majumder GC

    更新日期:2008-07-01 00:00:00

  • Effect of cryopreservation on the frequency of chromosomal abnormalities and sex ratio in human sperm.

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    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Martin RH,Chernos JE,Rademaker AW

    更新日期:1991-10-01 00:00:00

  • Endophyte-infected tall fescue diet alters gene expression in heifer luteal tissue as revealed by interspecies microarray analysis.

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    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Jones KL,King SS,Iqbal MJ

    更新日期:2004-02-01 00:00:00

  • Regulation of intracellular pH in capacitated human spermatozoa by a Na+/H+ exchanger.

    abstract::We previously demonstrated that the progesterone-(P) initiated human sperm acrosome reaction (AR) was dependent on the presence of extracellular Na+ (Na(-)0). Moreover, Na(-)0 depletion resulted in a decreased cytosolic pH (pHi), suggesting involvement of a Na(+)-dependent pHi regulatory mechanism during the P-initiat...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Garcia MA,Meizel S

    更新日期:1999-02-01 00:00:00

  • Molecular cloning and expression analysis of Fshr and Lhr in relation to Fshb and Lhb subunits during the period of temperature-dependent sex determination in pejerrey Odontesthes bonariensis.

    abstract::In this study, we cloned and characterized the follicle stimulating hormone receptor (Fshr) and luteinizing hormone receptor (Lhr) cDNAs of pejerrey Odontesthes bonariensis, a species with temperature-dependent sex determination (TSD), and analyzed their expression in relation to Fshb and Lhb subunits during gonadogen...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Shinoda T,Miranda LA,Okuma K,Hattori RS,Fernandino JI,Yoshizaki G,Somoza GM,Strüssmann CA

    更新日期:2010-06-01 00:00:00

  • Phosphatidylcholine signaling in response to CSF-1.

    abstract::The formation of cell membrane following CSF-1 stimulation of a macrophage cell line is coordinated with cell cycle progression. The majority of membrane phospholipid accumulates during the S phase and results from cell-cycle dependent oscillations in the rates of phosphatidylcholine biosynthesis and degradation. Both...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章,评审


    authors: Jackowski S,Xu XX,Rock CO

    更新日期:1997-01-01 00:00:00

  • EGF-like peptides mediate FSH-induced maturation of cumulus cell-enclosed mouse oocytes.

    abstract::This study was carried out to examine the participation of epidermal growth factor (EGF)-like peptides in the induction of germinal vesicle breakdown (GVB) in mouse cumulus cell-enclosed oocytes (CEO). The EGF-like peptide, amphiregulin (AR), dose-dependently stimulated meiotic resumption in CEO, but not denuded oocyt...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Downs SM,Chen J

    更新日期:2008-01-01 00:00:00

  • Targeted suppression of Has2 mRNA in mouse cumulus cell-oocyte complexes by adenovirus-mediated short-hairpin RNA expression.

    abstract::RNA interference (RNAi) is an effective tool for studying gene function in oocytes, but no studies have targeted somatic cells of primary cultured cumulus cell-oocyte complexes (COCs). This is probably due to difficulty in introducing RNAi-inducing molecules, such as a short-hairpin RNA (shRNA) gene, into COCs by comm...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Sugiura K,Su YQ,Eppig JJ

    更新日期:2009-06-01 00:00:00

  • Ca2+ signaling during maturation of cumulus-oocyte complex in mammals.

    abstract::Under the influence of gonadotropins or growth factors, a close cooperation develops between cumulus cells and the oocyte that is implicated in transmitting signals involved in maintaining or releasing the meiotic arrest in the oocyte. While cyclic adenosine 5'-monophosphate (cAMP) is a key molecule in maintaining the...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章,评审


    authors: Silvestre F,Boni R,Fissore RA,Tosti E

    更新日期:2011-10-01 00:00:00

  • Structural and functional modifications of the nucleolus during previtellogenic oocyte growth in the lizard Podarcis sicula.

    abstract::In the present study we analyse the nature and the functional significance of the spherical and fibrillo-granular structures appearing in the oocyte nucleus of the lizard Podarcis sicula, following the disappearance of the typical nucleolus. By LM and TEM approaches, we demonstrate that the fibrillo-granuli, containin...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Tammaro S,Andreuccetti P,Filosa S,Indolfi P,Prisco M,Motta CM

    更新日期:1998-12-01 00:00:00

  • Expression of cell death regulatory genes and limited apoptosis induction in avian blastodermal cells.

    abstract::Apoptosis is a well-established cellular mechanism for selective cell deletion during development. However, little is known about the expression of an apoptotic pathway and its role in determining the relative sensitivity of the early, pre-gastrula, avian embryo to stress-induced cell death. We examined the sensitivit...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Muscarella DE,Rachlinski MK,Bloom SE

    更新日期:1998-10-01 00:00:00

  • Prolactin receptor expression in the developing mouse embryo.

    abstract::We have examined the developmental pattern of prolactin receptor expression in the mouse by reverse transcription-polymerase chain reaction, in situ hybridization, and radioligand binding and have found two unexpected aspects of temporal regulation. First, high levels of prolactin receptor mRNA were detected in mouse ...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Tzeng SJ,Linzer DI

    更新日期:1997-09-01 00:00:00

  • Clusterin (SGP-2) in epididymal luminal fluid and its association with epididymal spermatozoa in androgen-deprived rats.

    abstract::Clusterin is a heterodimeric glycoprotein synthesized and secreted by rat Sertoli cells and epididymal epithelium. The goal of this study was to determine the presence of clusterin in the luminal fluid of the cauda epididymides and its association with the membranes of developing spermatozoa in the presence and absenc...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Mattmueller DR,Hinton BT

    更新日期:1992-05-01 00:00:00

  • Characterization of low Mr zona pellucida binding proteins from boar spermatozoa and seminal plasma.

    abstract::A group of low Mr (16 kDa-23 kDa) glycoproteins on ejaculated boar spermatozoa have been shown to have high affinity for homologous zona pellucida glycoproteins (ZPGPs). These ZPGP binding proteins are derived from seminal plasma as shown by their absence from epididymal spermatozoa and their presence in seminal plasm...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Parry RV,Barker PJ,Jones R

    更新日期:1992-09-01 00:00:00

  • Molecular cloning and characterization of SRG-L, a novel mouse gene developmentally expressed in spermatogenic cells.

    abstract::Full-length cDNA of a novel mouse gene upregulated in late stages of spermatogenic cells was cloned from mouse testis using overlapping RT-PCR and RACE. The mRNA of the gene was expressed mainly in diplotene/pachytene spermatocytes, round and elongating spermatids. We named this gene as SRG-L (Spermatogenesis Related ...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Ma Q,Wang H,Guo R,Wang H,Ge Y,Ma J,Xue S,Han D

    更新日期:2006-09-01 00:00:00

  • Ultrastructure of nuclear condensation and localization of DNA and proteins in spermatozoa of a dasyurid marsupial, Sminthopsis crassicaudata.

    abstract::In the dasyurid marsupial, Sminthopsis crassicaudata, the mature spermatozoon has an inner homogeneous (C1) and a peripheral indented (C2) region. Using DNase-gold conjugates, and biotinylated genomic DNA probes, DNA was found to occur in both C1 and C2 regions. The morphogenesis of the spermatozoon nucleus was invest...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Soon LL,Breed WG

    更新日期:1996-02-01 00:00:00

  • Effect of elevated Ca(2+) concentration in fusion/activation medium on the fusion and development of porcine fetal fibroblast nuclear transfer embryos.

    abstract::The present study examined the effect of elevated Ca(2+) concentration in fusion/activation medium on the fusion and development of fetal fibroblast nuclear transfer (NT) porcine embryos. Frozen-thawed and serum starved fetal fibroblasts were transferred into the perivitelline space of enucleated oocytes. Cell fusion ...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章


    authors: Cheong HT,Park KW,Im GS,Lai L,Sun QY,Day BN,Prather RS

    更新日期:2002-04-01 00:00:00