Activation mechanism and function of the MAP kinase cascade.

abstract：:Several animal and human studies have demonstrated that sex affects kinetics and metabolism during early embryo development. However, the mechanism governing these differences at the molecular level before the expression of the sex-determining gene SRY is unknown. We performed a systematic profiling of gene expression...

journal_title：Molecular reproduction and development

authors： Zhou Q,Wang T,Leng L,Zheng W,Huang J,Fang F,Yang L,Chen F,Lin G,Wang WJ,Kristiansen K

更新日期：2019-07-01 00:00:00

abstract：:These experiments were designed to test the effects of an electrofusion and an electroporation pulse on bovine sperm-hamster egg development. In experiment 1, single motile sperm were injected into the perivitelline space of each egg. A 4,500 V/cm, 30 microseconds fusion pulse (FP) was applied while sperm-egg membrane...

journal_title：Molecular reproduction and development

authors： Rickords LF,White KL,Wiltbank JN

更新日期：1990-10-01 00:00:00

abstract：:An organized microtubular cytoskeleton was discovered in the cytoplasm of Xenopus laevis oocytes. The microtubules were observed in 10- to 30-micron cryostat sections by indirect immunoperoxidase labeling using an antibody to tubulin. A gradual extraction of cells with a nonionic detergent was essential for good penet...

journal_title：Molecular reproduction and development

authors： Tourte M,Besse C,Mounolou JC

更新日期：1991-12-01 00:00:00

abstract：:The dysregulation of microRNAs (miRNAs) plays an important role in asthenozoospermia. This study evaluated the sperm microRNA-423-5p (miR-423-5p) expression in asthenozoospermia and normozoospermia, exploring the role of miR-423-5p in asthenozoospermia. Eighty participants were divided into asthenozoospermic (AZS, n =...

journal_title：Molecular reproduction and development

authors： Zhang R,Zuo Y,Cao S

更新日期：2021-01-28 00:00:00

abstract：:Nuclear transplantation studies demonstrated the importance of paternal contribution to embryogenesis. Paternal treatment with agents like cyclophosphamide and 5-azacytidine has been shown to cause an increase in pre-implantation loss (PIL) and post-implantation loss (POL). Studies from our laboratory have shown that ...

journal_title：Molecular reproduction and development

authors： Kedia N,Gill-Sharma MK,Parte P,Juneja HS,Balasinor N

更新日期：2004-09-01 00:00:00

abstract：:Artificial activation is required for successful intracytoplasmic sperm injection (ICSI) to induce haploidy pronuclear formation with extraction of second polar body. The present study showed that an additional treatment with Phorbol 12-myristate 13-acetate (PMA) followed by Ca(2+) ionophore treatment improved the rat...

journal_title：Molecular reproduction and development

authors： Ito J,Shimada M

更新日期：2005-01-01 00:00:00

abstract：:Meiotic progression requires the translation of maternal mRNAs in a strict temporal order. In isolated animal oocytes, translation of maternal mRNAs containing a cytoplasmic polyadenylation element (CPE), such as cyclin B, is activated by in vitro stimulation of meiotic resumption which induces phosphorylation of CPEB...

journal_title：Molecular reproduction and development

authors： Ochi H,Aoto S,Tachibana K,Hara M,Chiba K

更新日期：2016-01-01 00:00:00

abstract：:Nucleolar fine structure, "blebbing" activity of nuclear envelope, and activation of heterogeneous nuclear RNA (hnRNA) synthesis were studied in bovine reconstructed embryos obtained by electrofusion of a single eight-cell blastomere with an enucleated oocyte. Developmental progress of nucleolar fine structure and hnR...

journal_title：Molecular reproduction and development

authors： Kanka J,Fulka J Jr,Fulka J,Petr J

更新日期：1991-06-01 00:00:00

abstract：:Estrogen receptor (ER)- (alpha, beta, and both alpha and beta) and aromatase (Ar) knockout (KO) mice have been created to assess the biological effects of estrogens. This review article discusses the ovarian and uterine phenotypes of these mice. The data obtained have confirmed some older inferences about how the ster...

journal_title：Molecular reproduction and development

authors： Rosenfeld CS,Roberts RM,Lubahn DB

更新日期：2001-07-01 00:00:00

abstract：:The aim of the present study was to investigate the mechanism of androgen-mediated, prolonged sperm-storage in the female reproductive tract of the bat, Scotophilus heathii. The bat treated in vivo with flutamide, an androgen antagonist, showed loss of spermatozoa at the storage site, utero-tubal junction. Immunocytoc...

journal_title：Molecular reproduction and development

authors： Roy VK,Krishna A

更新日期：2011-07-01 00:00:00

abstract：:During bovine embryogenesis, bovine growth hormone (bGH) contributes to proliferation, differentiation, and modulation of embryo metabolism. Pituitary-specific transcription factor-1 (Pit-1) is a transcription factor that binds to promoters of GH, prolactin (PRL), and thyroid-stimulating hormone-beta (TSHbeta) encodin...

journal_title：Molecular reproduction and development

authors： Joudrey EM,Lechniak D,Petrik J,King WA

更新日期：2003-03-01 00:00:00

abstract：:Fragmentation occurs during early developmental stages of electrically activated oocytes and nuclear transfer (NT) embryos. It might contribute to the low developmental rate of porcine NT embryos. The present study was conducted to investigate whether the addition of sugars such as sorbitol or sucrose suppresses fragm...

journal_title：Molecular reproduction and development

authors： Im GS,Yang BS,Lai L,Liu Z,Hao Y,Prather RS

更新日期：2005-06-01 00:00:00

abstract：:Semen analysis lacks a functional component and best identifies extreme cases of infertility. The ganglioside GM1 is known to have functional roles during capacitation and acrosome exocytosis. Here, we assessed whether GM1 localization patterns (Cap-Score™) correspond with male fertility in different settings: Study 1...

journal_title：Molecular reproduction and development

authors： Cardona C,Neri QV,Simpson AJ,Moody MA,Ostermeier GC,Seaman EK,Paniza T,Rosenwaks Z,Palermo GD,Travis AJ

更新日期：2017-05-01 00:00:00

abstract：:Unlike in higher vertebrates, in fish it is not known whether the nerve supply of the testis can influence testicular functions or not. In addition to neurotransmitters, nerve terminals may release ATP and adenosine in the extracellular medium. On the assumption that these molecules might be released by fibers innerva...

journal_title：Molecular reproduction and development

authors： Loir M

更新日期：1999-08-01 00:00:00

abstract：:Inhibin α (Inha) gene expression is regulated, in rat granulosa cells, via a cyclic 3',5'-adenosine monophosphate (AMP)-response element (CRE) found in a region of the promoter that is homologous to the human INHA promoter. We previously found that during in vitro cytotrophoblast differentiation, human INHA gene expre...

journal_title：Molecular reproduction and development

authors： Depoix CL,Debiève F,Hubinont C

更新日期：2014-11-01 00:00:00

abstract：:The neomycin-resistant gene (neo(r)) is probably the most commonly used selectable marker gene in gene targeting and gene transfection research. In this study, the neo(r) gene construct was introduced into in vitro cultured goat foetal fibroblast cells (IV-5), and the cells were selected with 900 microg/ml G418. The G...

journal_title：Molecular reproduction and development

authors： Zou X,Wang Y,Cheng Y,Yang Y,Ju H,Tang H,Shen Y,Mu Z,Xu S,Du M

更新日期：2002-02-01 00:00:00

abstract：:Changes in spermatozoan ultrastructure have been studied during spermiogenesis of the slug Arion rufus (Gastropoda, Pulmonata, Stylommatophora). The ovotestis was investigated during the male stage, definite by the presence of spermatozoa. Some peculiar characteristics are shown by early spermatids: Around the nucleus...

journal_title：Molecular reproduction and development

authors： Pastisson C,Lacorre I

更新日期：1996-04-01 00:00:00

abstract：:Polycystic ovary syndrome (PCOS) is the most common endocrinological pathology among women of reproductive age, and is characterized by abnormalities in ovarian angiogenesis, among other features. Consistent with this association, follicular fluid (FF) concentration and ovarian expression of vascular endothelial growt...

journal_title：Molecular reproduction and development

authors： Scotti L,Parborell F,Irusta G,De Zuñiga I,Bisioli C,Pettorossi H,Tesone M,Abramovich D

更新日期：2014-08-01 00:00:00

abstract：:Oocytes enucleated at metaphase II stage can support reprogramming of transferred nucleus and further developing to term. However, the first polar body in mice sometimes migrates away from the original place of expulsion, so the chromosomes of the oocyte will displace from the first polar body. Thus, it is not always ...

journal_title：Molecular reproduction and development

authors： Wang MK,Liu JL,Li GP,Lian L,Chen DY

更新日期：2001-04-01 00:00:00

abstract：:Global warming represents a major stressful environmental condition that compromises the reproductive efficiency of animals and humans via a rise of body temperature above its physiological homeothermic point (heat stress [HS]). The injuries caused by HS on reproductive function involves both male and female component...

journal_title：Molecular reproduction and development

authors： Boni R

更新日期：2019-10-01 00:00:00

abstract：:FGF-4 appears to be the first fibroblast growth factor (FGF) expressed during embryogenesis, and its expression is critical for early mammalian development. FGF-4 is expressed in the embryonic cell lines, F9, D3, and NT2/D1; but its expression in these cells is repressed upon differentiation. Transcription of the FGF-...

journal_title：Molecular reproduction and development

authors： Lamb KA,Johnson LR,Rizzino A

更新日期：1997-11-01 00:00:00

abstract：:Three monoclonal antibodies against human protamines were obtained by immunization with total human basic nuclear proteins or purified protamine HP3. The specificity of antibodies was assessed by enzyme-linked immunosorbent assay (ELISA) and Western blot. They recognized three distinct epitopes: One was specific for t...

journal_title：Molecular reproduction and development

authors： Le Lannic G,Arkhis A,Vendrely E,Chevaillier P,Dadoune JP

更新日期：1993-09-01 00:00:00

abstract：:We describe the cloning and characterization of a partial cDNA, x121, that represents an RNA, which is localized in the animal hemisphere of Xenopus oocytes. This RNA is also detected in an animal to vegetal gradient during early cleavage stages. The x121 RNA titer decreases from fertilization through the gastrula sta...

journal_title：Molecular reproduction and development

authors： Kloc M,Reddy BA,Miller M,Eastman E,Etkin LD

更新日期：1991-04-01 00:00:00

abstract：:The present study examined the effect of elevated Ca(2+) concentration in fusion/activation medium on the fusion and development of fetal fibroblast nuclear transfer (NT) porcine embryos. Frozen-thawed and serum starved fetal fibroblasts were transferred into the perivitelline space of enucleated oocytes. Cell fusion ...

journal_title：Molecular reproduction and development

authors： Cheong HT,Park KW,Im GS,Lai L,Sun QY,Day BN,Prather RS

更新日期：2002-04-01 00:00:00

abstract：:Oocyte maturation is an important process required to achieve optimal oocyte quality, and later affects fertilization potential and subsequent embryo development. The maturation process includes synchronized nuclear and cytoplasmic remodeling, in which cytoskeletal and centrosome dynamics play an important role and si...

journal_title：Molecular reproduction and development

authors： Schatten H,Sun QY

更新日期：2011-10-01 00:00:00

abstract：:Irregular uterine bleeding is a major side effect of long-acting progestogen-only contraceptives in women, and is the primary reason women discontinue their use. In this study, a mouse model of endometrial breakdown was established using a subcutaneous progesterone implant to understand how irregular bleeding begins. ...

journal_title：Molecular reproduction and development

authors： Zhang GH,Cui LJ,Li AY,Zhang JP,Liu Y,Zhao JS,Xu XB,He B,Wang JD,Chu L,Li YF

更新日期：2016-09-01 00:00:00

abstract：:In the present study, immunogold labeling of ultrathin sections of ejaculated sperm was used to obtain insight into the ultrastructural localization and presumable function of type II cAMP-dependent protein kinase in sperm motion. In the flagellum, a human-specific isoform of the RII alpha subunit was located on the a...

journal_title：Molecular reproduction and development

authors： Pariset C,Weinman S

更新日期：1994-12-01 00:00:00

abstract：:We have cloned and sequenced cDNAs encoding autoantigen 1 (AA1), a testis-specific protein and the major autoantigen of the guinea pig sperm acrosome. The cDNA predicts a precursor protein of 244 amino acids including a 21 amino acid hydrophobic, secretory signal sequence. The mature polypeptide is predicted to have a...

journal_title：Molecular reproduction and development

authors： Foster JA,Gerton GL

更新日期：1996-06-01 00:00:00

abstract：:Expression of ovine interferon-tau (oIFNtau) genes, essential for the maternal recognition of pregnancy in ruminant ungulates, is restricted to the trophoblast and is not detected in any other cell types or tissues. Substantial secretion of oIFNtau starts on day 12-13 of pregnancy (day 0 = day of estrus), reaches the ...

journal_title：Molecular reproduction and development

authors： Nojima H,Nagaoka K,Christenson RK,Shiota K,Imakawa K

更新日期：2004-04-01 00:00:00

abstract：:Fertilization in invertebrates results in tyrosine (Tyr) phosphorylation of several egg proteins. However, the involvement of Tyr phosphorylation in mediating mammalian egg activation has not yet been investigated. Using an antibody specific for phosphotyrosine (P-Tyr), immunoblotting, and densitometric analysis, we f...

journal_title：Molecular reproduction and development

authors： Ben-Yosef D,Talmor A,Shwartz L,Granot Y,Shalgi R

更新日期：1998-02-01 00:00:00