MCL1 is a key regulator of steroidogenesis in mouse Leydig cells.

abstract：:Klinefelter syndrome (KS) is the most common genetic cause of human infertility, but the mechanism(s) responsible for its phenotype remain largely unknown. KS is associated with alterations in body composition and with a higher risk of developing metabolic diseases. We therefore hypothesized that KS men seeking fertil...

journal_title：Molecular reproduction and development

authors： Alves MG,Martins AD,Jarak I,Barros A,Silva J,Sousa M,Oliveira PF

更新日期：2016-03-01 00:00:00

abstract：:The objective of this research was to evaluate if DNA hypomethylation in cells used as karyoplasts would improve development of bovine nuclear transplantation (NT) embryos. DNA from serum-fed (SF), serum-starved (SS), and 1, or 5 microM 5-azacytidine (5-aza-CR) treated cells was digested with a methylation sensitive e...

journal_title：Molecular reproduction and development

authors： Jones KL,Hill J,Shin TY,Lui L,Westhusin M

更新日期：2001-10-01 00:00:00

abstract：:The present study investigated the effects of triiodothyronine (T3) on pituitary gonadotropin (GTH) subunits, thyroid stimulating hormone (TSH) β subunit, and growth hormone (GH) mRNA levels, as well as gonadal steroid secretion during different stages of reproduction in goldfish. Goldfish pituitary cells cultured wit...

journal_title：Molecular reproduction and development

authors： Allan ER,Habibi HR

更新日期：2012-09-01 00:00:00

abstract：:The present study examined the effect of elevated Ca(2+) concentration in fusion/activation medium on the fusion and development of fetal fibroblast nuclear transfer (NT) porcine embryos. Frozen-thawed and serum starved fetal fibroblasts were transferred into the perivitelline space of enucleated oocytes. Cell fusion ...

journal_title：Molecular reproduction and development

authors： Cheong HT,Park KW,Im GS,Lai L,Sun QY,Day BN,Prather RS

更新日期：2002-04-01 00:00:00

abstract：:The lack of a paternal genome in parthenogenetic embryos clearly limits their postimplantation development, but apparently not their preimplantation development, since morphologically normal blastocysts can be formed. The cleavage rate of these embryos during the preimplantation period gives a better indication of the...

journal_title：Molecular reproduction and development

authors： Henery CC,Kaufman MH

更新日期：1992-04-01 00:00:00

abstract：:The analysis of differences in gene expression, responding to cryopreservation may explain some of the observed differences in further development of the preimplantation stage embryos. The aim of this study was to create a link, for the first time, between morphological/developmental observations and gene activity cha...

journal_title：Molecular reproduction and development

authors： Boonkusol D,Gal AB,Bodo S,Gorhony B,Kitiyanant Y,Dinnyes A

更新日期：2006-06-01 00:00:00

abstract：:We previously demonstrated that the progesterone-(P) initiated human sperm acrosome reaction (AR) was dependent on the presence of extracellular Na+ (Na(-)0). Moreover, Na(-)0 depletion resulted in a decreased cytosolic pH (pHi), suggesting involvement of a Na(+)-dependent pHi regulatory mechanism during the P-initiat...

journal_title：Molecular reproduction and development

authors： Garcia MA,Meizel S

更新日期：1999-02-01 00:00:00

abstract：:Exposing day 5 bovine morulae to reactive oxygen species induces a delayed degeneration of some blastocysts on day 8 post-insemination (pi) but without affecting the blastocyst rates. The aim of this study was to characterize the resisting and the degenerating population of blastocysts. The kinetics of degeneration of...

journal_title：Molecular reproduction and development

authors： Feugang JM,Donnay I,Mermillod P,Marchandise J,Lequarre AS

更新日期：2005-07-01 00:00:00

abstract：:Both ovarian and pituitary hormones are required for the pubertal development of the mouse mammary gland. Estradiol directs ductal elongation and branching, while progesterone leads to tertiary branching and alveolar development. The purpose of this investigation was to identify estrogen-responsive genes associated wi...

journal_title：Molecular reproduction and development

authors： Deroo BJ,Hewitt SC,Collins JB,Grissom SF,Hamilton KJ,Korach KS

更新日期：2009-08-01 00:00:00

abstract：:Human ejaculate spermatozoa contain multiple mRNA species carried over from earlier stages in spermatogenesis. To date, gene-specific RT-PCR or in situ hybridization has detected transcripts for beta-actin, heat shock proteins (HSP) 70 and 90, protamines (PRM) 1 and 2, transition protein (TNp) 2, HLA II, beta-integrin...

journal_title：Molecular reproduction and development

authors： Miller D

更新日期：2000-06-01 00:00:00

abstract：:The production of chimeras, by use of cell transplantation, has proved to be highly valuable in studies of development by providing insights into cell fate, differentiation, and developmental potential. So far, chimeric honeybees have been created by nuclear transfer technologies. We have developed protocols to produc...

journal_title：Molecular reproduction and development

authors： Bergem M,Norberg K,Roseth A,Meuwissen T,Lien S,Aamodt RH

更新日期：2006-04-01 00:00:00

abstract：:Myricetin is a natural flavonoid, particularly enriched in red wines, whose occurrence is widespread among plants. Despite extensive research, the beneficial effects of Myricetin on human health are still controversial. Here, we tested the estrogen-like effect of the phytoestrogen Myricetin on human ejaculated sperm b...

journal_title：Molecular reproduction and development

authors： Aquila S,Santoro M,De Amicis F,Guido C,Bonofiglio D,Lanzino M,Cesario MG,Perrotta I,Sisci D,Morelli C

更新日期：2013-02-01 00:00:00

abstract：:Fresh cytosols extracted from unfertilized amphibian eggs contain a cytostatic factor (CSF) which arrests the cell cycle at metaphase when microinjected into cleaving blastomeres. This CSF is sensitive to Ca2+, and is designated primary CSF (1 degree CSF). During storage of Ca(2+)-containing cytosols at 2 degrees C, s...

journal_title：Molecular reproduction and development

authors： Zhang J,Masui Y

更新日期：1997-07-01 00:00:00

abstract：:This study was designed to examine the recovery of mitochondrial function and endogenous antioxidant systems in vitrified oocytes during extended incubations. After 16 hr of in vitro maturation, bovine meiosis-II oocytes were vitrified, and then surviving oocytes were cultured an additional 8 hr. ATP content, ATP synt...

journal_title：Molecular reproduction and development

authors： Zhao XM,Du WH,Wang D,Hao HS,Liu Y,Qin T,Zhu HB

更新日期：2011-12-01 00:00:00

abstract：:Exposure to nickel has been shown to cause damage to the testis in several animal models. It is not known if the testis expresses protein(s) that can bind nickel. To test this, we used a nickel-binding assay to isolate testicular nickel-binding proteins. We identified glutamate-ammonia ligase (GLUL) as a prominent nic...

journal_title：Molecular reproduction and development

authors： Sun Y,Ou Y,Cheng M,Ruan Y,van der Hoorn FA

更新日期：2011-02-01 00:00:00

abstract：:The present study was designed to investigate the effect of vitrification on mitochondrial distribution, membrane potential (Deltapsi) and microtubule distribution in mouse 2-PN embryos, as well as to document the relationship between mitochondrial distribution and developmental ability of those embryos. Mitochondrial...

journal_title：Molecular reproduction and development

authors： Zhao XM,Fu XW,Hou YP,Yan CL,Suo L,Wang YP,Zhu HB,Dinnyés A,Zhu SE

更新日期：2009-11-01 00:00:00

abstract：:Abnormal oocyte spindle due to the improper function of ooplasm is associated with female infertility of advanced maternal age. A possible way to overcome this problem is to transfer an oocyte germinal vesicle (GV) which contains genetic materials of a patient with a history of poor embryo development to the cytoplast...

journal_title：Molecular reproduction and development

authors： Li GP,Chen DY,Lian L,Sun QY,Wang MK,Liu JL,Li JS,Han ZM

更新日期：2001-02-01 00:00:00

abstract：:Abattoir-derived oocytes were exposed to a concentrated cryoprotectant solution (DAP213: 2 M DMSO, 1 M acetamide, 3 M propanediol, and 10% FCS in TCM199) for 1.5 or 5 min at the germinal vesicle (GV) stage or after maturation in vitro (IVM). Their viability was assessed by in vitro fertilization (IVF) and culture (IVC...

journal_title：Molecular reproduction and development

authors： Fuku EJ,Liu J,Downey BR

更新日期：1995-02-01 00:00:00

abstract：:Embryonal carcinoma (EC) cells are used widely as a model system for studying the expression of developmentally regulated genes, in particular genes that are regulated at the transcriptional level when EC cells differentiate. This review focuses on the molecular mechanisms that govern the transcription of the fibrobla...

journal_title：Molecular reproduction and development

authors： Lamb KA,Rizzino A

更新日期：1998-10-01 00:00:00

abstract：:Previously we demonstrated that lines of transgenic mice carrying the herpes simplex type 1 virus thymidine kinase (HSV1-tk) reporter gene are male-sterile. Ectopic transcription of the HSV1-tk reporter in the testis was initiated downstream of the normal translation initiation codon and truncated proteins consistent ...

journal_title：Molecular reproduction and development

authors： Ellison AR,Wallace H,al-Shawi R,Bishop JO

更新日期：1995-08-01 00:00:00

abstract：:The aim of this study was to evaluate the role of adiponectin in the delayed embryonic development of Cynopterus sphinx. Adiponectin receptor (ADIPOR1) abundance was first observed to be lower during the delayed versus non-delayed periods of utero-embryonic unit development. The effects of adiponectin treatment on emb...

journal_title：Molecular reproduction and development

authors： Anuradha,Krishna A

更新日期：2014-12-01 00:00:00

abstract：:The mouse metallothionein (MT) gene family consists of four known members (MT-I through IV) clustered on chromosome 8. Studies reported herein examine the expression and regulation of the MT-III and MT-IV genes in specific cell types in the maternal reproductive tract, developing embryo, and fetus known to express the...

journal_title：Molecular reproduction and development

authors： Liang L,Fu K,Lee DK,Sobieski RJ,Dalton T,Andrews GK

更新日期：1996-01-01 00:00:00

abstract：:The nuclear matrix is thought to be responsible for DNA organization, DNA replication, RNA synthesis, and RNA processing. We have looked for the presence of nuclear matrix antigens during early mouse embryogenesis. Antibodies to peripheral and interior antigens (P1, Pl1, Pl2, and lamin B) were used to immunolocalize n...

journal_title：Molecular reproduction and development

authors： Prather RS,Schatten G

更新日期：1992-07-01 00:00:00

abstract：:The mussel Mytilus edulis can be used as model to study the molecular basis of reproductive isolation because this species maintains its species integrity, despite of hybridizing in zones of contact with the closely related species M. trossulus or M. galloprovincialis. This study uses selective antibody production by ...

journal_title：Molecular reproduction and development

authors： Stuckas H,Messerschmidt K,Putzler S,Baumann O,Schenk JA,Tiedemann R,Micheel B

更新日期：2009-01-01 00:00:00

abstract：:Follicle-stimulating hormone (FSH) regulates folliculogenesis in the ovary and spermatogenesis in the testis via specific, high affinity membrane-bound receptors (FSHR). To assess the role of FSHR gene expression in regulating expression of FSHR protein in the plasma membrane, the effects of a porcine FSHR cDNA antise...

journal_title：Molecular reproduction and development

authors： Zhu C,Wang Y,Nixon MD,La Barbera AR

更新日期：2003-06-01 00:00:00

abstract：:Activation of the mammalian egg results in cortical reaction (CR), which is correlated with an increase in intracellular Ca2+ concentration and PKC activation. The CR is a gradual rather then an "all or none" response, and can be regulated by different concentrations of parthenogenetic activators. To evaluate the biol...

journal_title：Molecular reproduction and development

authors： Raz T,Skutelsky E,Amihai D,Hammel I,Shalgi R

更新日期：1998-11-01 00:00:00

abstract：:During the molecular cloning of the ovine testicular follicle-stimulating (FSH) receptor that couples to the Gs-type effector systems, we discovered novel cDNA clones that were highly homologous. Some of these clones contained an insert of 1,584 bp, which consisted of a divergent 3' region spliced with a 5' region tha...

journal_title：Molecular reproduction and development

authors： Yarney TA,Jiang L,Khan H,MacDonald EA,Laird DW,Sairam MR

更新日期：1997-12-01 00:00:00

abstract：:Spermatozoa from the sperm-rich fractions of the semen of 6 beagle dogs were capacitated and the effect of both zona pellucida (ZP) proteins and progesterone on calcium flux and the acrosome reaction measured. Sperm calcium flux was determined using the dual wavelength calcium probe indo-1/AM (6 microM) in a flow cyto...

journal_title：Molecular reproduction and development

authors： Brewis IA,Morton IE,Moore HD,England GC

更新日期：2001-12-01 00:00:00

abstract：:To establish reliable criteria for the evaluation of nuclear donor embryos, we studied the effect of cell number and cell size of in vitro produced day 6 donor morulae on the rate of blastocyst formation following nuclear transfer to in vitro matured oocytes. In experiment 1, donor embryos were divided into three grou...

journal_title：Molecular reproduction and development

authors： Zakhartchenko V,Wolf E,Palma GA,Brem G

更新日期：1995-09-01 00:00:00

abstract：:The present study was conducted to investigate the mechanisms underlying fluid movement across the trophectoderm during blastocyst formation by determining whether aquaporins (AQPs) are expressed during early mammalian development. AQPs belong to a family of major intrinsic membrane proteins and function as molecular ...

journal_title：Molecular reproduction and development

authors： Offenberg H,Barcroft LC,Caveney A,Viuff D,Thomsen PD,Watson AJ

更新日期：2000-12-01 00:00:00