Haprin-deficient spermatozoa are incapable of in vitro fertilization.

abstract：:During the molecular cloning of the ovine testicular follicle-stimulating (FSH) receptor that couples to the Gs-type effector systems, we discovered novel cDNA clones that were highly homologous. Some of these clones contained an insert of 1,584 bp, which consisted of a divergent 3' region spliced with a 5' region tha...

journal_title：Molecular reproduction and development

authors： Yarney TA,Jiang L,Khan H,MacDonald EA,Laird DW,Sairam MR

更新日期：1997-12-01 00:00:00

abstract：:Mitogen-activated protein kinase (MAPK) plays a crucial role in meiotic maturation of mouse oocytes. In order to understand the mechanism by which MAPK regulates meiotic maturation, we examined the effects of the MAPK pathway inhibitor U0126 on microtubule organization, gamma-tubulin and nuclear mitotic apparatus prot...

journal_title：Molecular reproduction and development

authors： Lee SE,Kim JH,Kim NH

更新日期：2007-07-01 00:00:00

abstract：:The fibrous sheath is a major cytoskeletal structure in the principal piece of the mammalian sperm flagellum. Two peptide sequences obtained from a tryptic digest of mouse fibrous sheath proteins exhibited high homology with mu-class glutathione S-transferases (GSTs). Using a DNA probe amplified from degenerate polyme...

journal_title：Molecular reproduction and development

authors： Fulcher KD,Welch JE,Klapper DG,O'Brien DA,Eddy EM

更新日期：1995-12-01 00:00:00

abstract：:Zona pellucida (ZP) glycoproteins have been proposed as candidate antigens for an immunocontraceptive vaccine. The efficacy of such a vaccine has to be evaluated in nonhuman primates, thus necessitating the characterization of their ZP glycoproteins. A bonnet monkey (Macaca radiata) ovarian cDNA lambda gt11 library wa...

journal_title：Molecular reproduction and development

authors： Jethanandani P,Santhanam R,Gupta SK

更新日期：1998-06-01 00:00:00

abstract：:An organized microtubular cytoskeleton was discovered in the cytoplasm of Xenopus laevis oocytes. The microtubules were observed in 10- to 30-micron cryostat sections by indirect immunoperoxidase labeling using an antibody to tubulin. A gradual extraction of cells with a nonionic detergent was essential for good penet...

journal_title：Molecular reproduction and development

authors： Tourte M,Besse C,Mounolou JC

更新日期：1991-12-01 00:00:00

abstract：:Dead-end1 (Dnd1) expression is restricted to the vertebrate germline where it is believed to activate translation of messenger RNAs (mRNAs) required to protect and promote that unique lineage. Nanos1 is one such germline mRNA whose translation is blocked by a secondary mRNA structure within the open reading frame (ORF...

journal_title：Molecular reproduction and development

authors： Aguero T,Jin Z,Owens D,Malhotra A,Newman K,Yang J,King ML

更新日期：2018-12-01 00:00:00

abstract：:Plac1 is a recently identified, X-linked gene whose expression is restricted primarily to cells of the trophoblast lineage. It localizes to a chromosomal locus previously implicated in placental growth. We therefore sought to determine if Plac1 is necessary for placental and embryonic development by examining a mutant...

journal_title：Molecular reproduction and development

authors： Jackman SM,Kong X,Fant ME

更新日期：2012-08-01 00:00:00

abstract：:cDNA clones encoding stage-and/or space-specific genes in porcine ovarian follicles have been investigated by differential screening of cDNA libraries of granulosa cells and corpus luteum. A clone corresponding to mRNA preferentially expressed in the functional stage of corpus luteum was found to encode a protein simi...

journal_title：Molecular reproduction and development

authors： Tanaka T,Itahana K,Andoh N,Takeya T,Sato E

更新日期：1995-10-01 00:00:00

abstract：:The possibility to isolate canine mesenchymal stem cells (MSCs) from foetal adnexa is interesting since several canine genetic disorders are reported to resemble similar dysfunctions in humans. In this study, we successfully isolated, cytogenetically and molecularly characterized, and followed the differentiation pote...

journal_title：Molecular reproduction and development

authors： Filioli Uranio M,Valentini L,Lange-Consiglio A,Caira M,Guaricci AC,L'Abbate A,Catacchio CR,Ventura M,Cremonesi F,Dell'Aquila ME

更新日期：2011-05-01 00:00:00

abstract：:In this study, we sought to determine the mechanism of early sex reversal in a teleost by applying 4 hr feminization treatments to XY (17α-ethynylestradiol 2000 μg L(-1) ) and YY (6500 μg L(-1) ) Nile tilapia embryos on the first day post-fertilization (dpf). We then searched for changes in the expression profiles of ...

journal_title：Molecular reproduction and development

authors： Gennotte V,Mélard C,D'Cotta H,Baroiller JF,Rougeot C

更新日期：2014-12-01 00:00:00

abstract：:The purposes of this study were to establish and to explore the biological basis of the chronic stress-induced premature ovarian failure (POF) model and to explore the therapeutic effects of the traditional Chinese medicine Muniziqi. Sexually matured female Sprague-Dawley rats were fed with spinach and cilantro in col...

journal_title：Molecular reproduction and development

authors： Keremu A,Yaoliwasi A,Tuerhong M,Kadeer N,Heyi,Yiming A,Yilike X

更新日期：2019-02-01 00:00:00

abstract：:Bicarbonate/CO2 is believed to be the key in vitro effector of sperm capacitation, a process which induces major changes in the sperm plasma membrane in preparation for fertilization. In a flow cytometric study, we examined the effect of bicarbonate on boar spermatozoa using merocyanine, an impermeant lipophilic probe...

journal_title：Molecular reproduction and development

authors： Harrison RA,Ashworth PJ,Miller NG

更新日期：1996-11-01 00:00:00

abstract：:In the lizard Podarcis sicula, the major vitellogenin (VTG)-derived yolk proteins, lipovitellins and phosvitins, were extracted from the yolk globules of laid and fertilized eggs at different periods of incubation up to 44 days close to hatching. Embryonic development was almost over at this time. Yolk proteins were i...

journal_title：Molecular reproduction and development

authors： Romano M,Rosanova P,Anteo C,Limatola E

更新日期：2002-11-01 00:00:00

abstract：:RNA interference (RNAi) is an effective tool for studying gene function in oocytes, but no studies have targeted somatic cells of primary cultured cumulus cell-oocyte complexes (COCs). This is probably due to difficulty in introducing RNAi-inducing molecules, such as a short-hairpin RNA (shRNA) gene, into COCs by comm...

journal_title：Molecular reproduction and development

authors： Sugiura K,Su YQ,Eppig JJ

更新日期：2009-06-01 00:00:00

abstract：:The present study was designed to investigate the effect of vitrification on mitochondrial distribution, membrane potential (Deltapsi) and microtubule distribution in mouse 2-PN embryos, as well as to document the relationship between mitochondrial distribution and developmental ability of those embryos. Mitochondrial...

journal_title：Molecular reproduction and development

authors： Zhao XM,Fu XW,Hou YP,Yan CL,Suo L,Wang YP,Zhu HB,Dinnyés A,Zhu SE

更新日期：2009-11-01 00:00:00

abstract：:Many studies have shown a correlation between transferrin (Tf) concentration and sperm yield in several mammalian species. We have used transgenic mice expressing human Tf (hTf) to investigate if overexpression of Tf increases the efficiency of mouse spermatogenesis. We demonstrated that a 36% increase of Tf does not ...

journal_title：Molecular reproduction and development

authors： Lécureuil C,Staub C,Fouchécourt S,Maurel MC,Fontaine I,Martinat N,Gauthier C,Daudignon A,Delaleu B,Sow A,Jégou B,Guillou F

更新日期：2007-02-01 00:00:00

abstract：:During bovine embryogenesis, bovine growth hormone (bGH) contributes to proliferation, differentiation, and modulation of embryo metabolism. Pituitary-specific transcription factor-1 (Pit-1) is a transcription factor that binds to promoters of GH, prolactin (PRL), and thyroid-stimulating hormone-beta (TSHbeta) encodin...

journal_title：Molecular reproduction and development

authors： Joudrey EM,Lechniak D,Petrik J,King WA

更新日期：2003-03-01 00:00:00

abstract：:In mammals, Sry, Sox9, and M33 act as regulators at a chromatin level and promote the maturation of embryonic gonads into testes. Recently, it was shown that transcriptional regulation by DNA methylation plays crucial roles in gene expression during the differentiation and development of various cell types. To determi...

journal_title：Molecular reproduction and development

authors： Mizukami T,Kanai Y,Fujisawa M,Kanai-Azuma M,Kurohmaru M,Hayashi Y

更新日期：2008-06-01 00:00:00

abstract：:The objective of the present study was to investigate the ultrastructural consequences of vitrification of bovine oocytes at the metaphase II (MII) stage by the so-called "Open Pulled Straw" method. Oocytes were matured in vitro for 22 hr and cryopreserved by vitrification. After warming and additional 2 hr of culture...

journal_title：Molecular reproduction and development

authors： Hyttel P,Vajta G,Callesen H

更新日期：2000-05-01 00:00:00

abstract：:In newt testis, spermatocytes never appear during winter, because secondary spermatogonia die by apoptosis just before meiosis. In the current study, we examined the effect of low temperatures on spermatogenesis. Incubation of newts at low temperatures (8, 12, 15 degrees C) induced defects in spermatogenesis in a temp...

journal_title：Molecular reproduction and development

authors： Yazawa T,Nakayama Y,Fujimoto K,Matsuda Y,Abe K,Kitano T,Abé S,Yamamoto T

更新日期：2003-09-01 00:00:00

abstract：:The mitochondrial probe 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolyl-carbocyan ine iodide (JC-1) not only identifies mitochondria exhibiting low membrane potentials by the emission of green fluorescence (range, 510-520 nm) but also differentiates these from mitochondria exhibiting relatively high membrane ...

journal_title：Molecular reproduction and development

authors： Garner DL,Thomas CA

更新日期：1999-06-01 00:00:00

abstract：:Germ cells occupy a unique position in animal reproduction, development, and evolution. In sexually reproducing animals, only they can produce gametes and contribute genetically to subsequent generations. Nonetheless, germ line specification during embryogenesis is conceptually the same as the specification of any som...

journal_title：Molecular reproduction and development

authors： Ewen-Campen B,Schwager EE,Extavour CG

更新日期：2010-01-01 00:00:00

abstract：:The vascular cell responses to the type 1, 2, and 3 isoforms of transforming growth factor-beta (TGF-beta 1, TGF-beta 2, TGF-beta 3) were studied using bovine aortic endothelial (BAECs) and smooth muscle cells (BASMC3) as well as rat epididymal fat pad microvascular endothelia (RFCs). Three distinct bioassays indicate...

journal_title：Molecular reproduction and development

authors： Madri JA,Bell L,Merwin JR

更新日期：1992-06-01 00:00:00

abstract：:Clusterin is a heterodimeric glycoprotein synthesized and secreted by rat Sertoli cells and epididymal epithelium. The goal of this study was to determine the presence of clusterin in the luminal fluid of the cauda epididymides and its association with the membranes of developing spermatozoa in the presence and absenc...

journal_title：Molecular reproduction and development

authors： Mattmueller DR,Hinton BT

更新日期：1992-05-01 00:00:00

abstract：:Three monoclonal antibodies against human protamines were obtained by immunization with total human basic nuclear proteins or purified protamine HP3. The specificity of antibodies was assessed by enzyme-linked immunosorbent assay (ELISA) and Western blot. They recognized three distinct epitopes: One was specific for t...

journal_title：Molecular reproduction and development

authors： Le Lannic G,Arkhis A,Vendrely E,Chevaillier P,Dadoune JP

更新日期：1993-09-01 00:00:00

abstract：:Heat shock proteins (HSPs) are known to play an important role not only in various stress conditions such as exposure to heat shock, but also in normal development and/or differentiation. The role of small heat shock proteins such as HSP25 in early embryo development remains largely unknown. In the present study, we e...

journal_title：Molecular reproduction and development

authors： Kim M,Geum D,Khang I,Park YM,Kang BM,Lee KA,Kim K

更新日期：2002-01-01 00:00:00

abstract：:In the present study propidium iodide was used as a fluorescent dye to stain DNA of cells of hamster testicular origin and fluorescent intensities were analyzed by flow cytometry. We used hamster testicular cells from the first spermatogenic wave to observe the consecutive appearance of the different types of cells du...

journal_title：Molecular reproduction and development

authors： Golan R,Weissenberg R,Oschry Y,Shochat L,Lewin LM

更新日期：2000-02-01 00:00:00

abstract：:The presence of retinoic acid (RA) during in vitro maturation (IVM) improves bovine oocyte quality and developmental potential. In this work, we investigated the underlying molecular mechanisms. Cumulus-oocyte complexes were meiotically arrested by roscovitine and/or matured in defined medium containing RA, 1% ethanol...

journal_title：Molecular reproduction and development

authors： Gomez E,Rodríguez A,Goyache F,Díez C,José Royo L,Moreira PN,Néstor Caamaño J,Morán E,Gutiérrez-Adán A

更新日期：2004-09-01 00:00:00

abstract：:Acrosin (ACR), a serine proteinase located in the acrosome of the sperm, has been presumed to be involved in the recognition and binding of the sperm to the zona pellucida of the ovum and the sperm penetration through the zona pellucida. To examine the function of acrosin in vivo, we have generated mice carrying a mut...

journal_title：Molecular reproduction and development

authors： Adham IM,Nayernia K,Engel W

更新日期：1997-03-01 00:00:00

abstract：:Hyperactivated motility was studied in guinea pig spermatozoa. In the presence of the local anesthetic procaine, a high number of sperm cells (64%) showed hyperactivation when incubated in minimal culture medium with pyruvate, lactate, and glucose. Hyperactivated motility was dependent on glucose in the medium. Sperm ...

journal_title：Molecular reproduction and development

authors： Mújica A,Neri-Bazan L,Tash JS,Uribe S

更新日期：1994-07-01 00:00:00