Evaluation of unrestricted somatic stem cells as a feeder layer to support undifferentiated embryonic stem cells.

abstract：:Triosephosphate isomerase 1 (TPI1) is a member of the glycolytic pathway, which is a critical source of energy for motility in mouse sperm. By immunoblotting, we detected two male, germ line-specific TPI1 bands (Mr 33,400 and 30,800) as well as the somatic-type band (Mr 27,700). Although all three bands were observed ...

journal_title：Molecular reproduction and development

authors： Ijiri TW,Vadnais ML,Lin AM,Huang AP,Cao W,Merdiushev T,Gerton GL

更新日期：2013-10-01 00:00:00

abstract：:Previously we demonstrated that lines of transgenic mice carrying the herpes simplex type 1 virus thymidine kinase (HSV1-tk) reporter gene are male-sterile. Ectopic transcription of the HSV1-tk reporter in the testis was initiated downstream of the normal translation initiation codon and truncated proteins consistent ...

journal_title：Molecular reproduction and development

authors： Ellison AR,Wallace H,al-Shawi R,Bishop JO

更新日期：1995-08-01 00:00:00

abstract：:Mouse and rabbit preimplantation embryos with intact zona pellucida were incubated for 3 hr with DNA-carrying constructs containing insulin as an internalizable ligand: (insulin-polylysine)-DNA and (insulin-polylysine)-DNA-(streptavidin-polylysine)-(biotinylated adenovirus). Video-intensified microscopy demonstrated t...

journal_title：Molecular reproduction and development

authors： Ivanova MM,Rosenkranz AA,Smirnova OA,Nikitin VA,Sobolev AS,Landa V,Naroditsky BS,Ernst LK

更新日期：1999-10-01 00:00:00

abstract：:In vitro culture of mammalian preimplantation embryos is associated with various developmental disorders such as retardation in development and cell damage. The molecular mechanisms underlying these processes are poorly understood. One of the possible reasons may be the unphysiologically high oxygen concentration used...

journal_title：Molecular reproduction and development

authors： Koerber S,Santos AN,Tetens F,Küchenhoff A,Fischer B

更新日期：1998-04-01 00:00:00

abstract：:The homeobox-containing gene family plays a pivotal role in regulating, patterning, and axial morphogenesis in the developing embryo. But there is still very little known about the expression and function of these genes in mammalian oocytes and preimplantation stage embryos. In this study we have used degenerate prime...

journal_title：Molecular reproduction and development

authors： Ponsuksili S,Wimmers K,Adjaye J,Schellander K

更新日期：2001-11-01 00:00:00

abstract：:The possibility to isolate canine mesenchymal stem cells (MSCs) from foetal adnexa is interesting since several canine genetic disorders are reported to resemble similar dysfunctions in humans. In this study, we successfully isolated, cytogenetically and molecularly characterized, and followed the differentiation pote...

journal_title：Molecular reproduction and development

authors： Filioli Uranio M,Valentini L,Lange-Consiglio A,Caira M,Guaricci AC,L'Abbate A,Catacchio CR,Ventura M,Cremonesi F,Dell'Aquila ME

更新日期：2011-05-01 00:00:00

abstract：:Collagen is denatured in the gradual cervical ripening process during late pregnancy, already before the onset of final cervical ripening at parturition. Matrix Metallo Proteinases (MMPs) might be responsible for this process. To investigate the presence and potential function of MMPs at the different stages of the ri...

journal_title：Molecular reproduction and development

authors： van Engelen E,Breeveld-Dwarkasing VN,Taverne MA,Everts ME,van der Weijden GC,Rutten VP

更新日期：2008-11-01 00:00:00

abstract：:Glycogen synthase kinase-3 (GSK-3) is a highly conserved serine/threonine protein kinase implicated in diverse cellular processes. Activity of GSK-3 is essential for meiotic chromatin segregation in oocytes, yet expression and/or function of GSK-3 have not been reported in mammalian preimplantation embryos. Objectives...

journal_title：Molecular reproduction and development

authors： Acevedo N,Wang X,Dunn RL,Smith GD

更新日期：2007-02-01 00:00:00

abstract：:Proteins synthesized by DDK mice embryos were analyzed by 2D electrophoresis and a new egg-specific polypeptide, D14, was identified. The protein is characterized by its high rate of synthesis and electrophoretic properties (MW 36,500, pl greater than 8). The synthesis of D14 is strictly developmentally regulated: sta...

journal_title：Molecular reproduction and development

authors： Richoux V,Renard JP,Babinet C

更新日期：1991-03-01 00:00:00

abstract：:Germinal vesicle (GV) breakdown in mammalian oocytes is regulated by the activation of maturation promoting factor (MPF). We investigated a specific cdc2 kinase inhibitor, roscovitine, to maintain pig oocytes in the GV stage. Cumulus-oocyte complexes (COCs) were aspirated from slaughterhouse ovaries and cultured for 4...

journal_title：Molecular reproduction and development

authors： Ju JC,Tsay C,Ruan CW

更新日期：2003-04-01 00:00:00

abstract：:A mouse cDNA encoding a putative DNA-binding protein of the zinc-finger type was isolated from an E8.5 mouse embryonic cDNA library. Sequence comparison revealed a high degree of homology between this mouse cDNA and the human and rat orphan receptor Tr2-11 isolated from prostate cDNA libraries. This transcript was det...

journal_title：Molecular reproduction and development

authors： Lee CH,Chang L,Wei LN

更新日期：1996-07-01 00:00:00

abstract：:Transcription factors inhibit or assist RNA polymerases in the initiation and maintenance of transcription; however, the cell specific expression and roles of transcription factors within bovine ovarian follicles during development are unknown. The aim of present study was to determine if the expression of transcripti...

journal_title：Molecular reproduction and development

authors： Zielak AE,Canty MJ,Forde N,Coussens PM,Smith GW,Lonergan P,Ireland JJ,Evans AC

更新日期：2008-05-01 00:00:00

abstract：:It was reported that a hamster protein, called "oscillin," with a sequence related to that of an Escherichia coli GNPDA triggered Ca(2+) oscillations in mammalian oocytes when introduced into their cytoplasm upon fertilization. Recently, it was shown that GNPDA/oscillin is ubiquitously expressed in rat tissues and tha...

journal_title：Molecular reproduction and development

authors： Amireault P,Dubé F

更新日期：2000-07-01 00:00:00

abstract：:The Src family kinase (SFK) is important in normal cell cycle control. However, its role in meiotic maturation in mammalian has not been examined. We used confocal microscope immunofluorescence to examine the in vitro dynamics of the subcellular distribution of SFK during the mouse oocyte meiotic maturation and furthe...

journal_title：Molecular reproduction and development

authors： Zheng KG,Meng XQ,Yang Y,Yu YS,Liu DC,Li YL

更新日期：2007-01-01 00:00:00

abstract：:Proteins in the seminal fluid of animals with internal fertilization effect numerous responses in mated females that impact both male and female fertility. Among these proteins is the highly represented class of proteolysis regulators (proteases and their inhibitors). Though proteolysis regulators have now been identi...

journal_title：Molecular reproduction and development

authors： Laflamme BA,Wolfner MF

更新日期：2013-02-01 00:00:00

abstract：:Abattoir-derived oocytes were exposed to a concentrated cryoprotectant solution (DAP213: 2 M DMSO, 1 M acetamide, 3 M propanediol, and 10% FCS in TCM199) for 1.5 or 5 min at the germinal vesicle (GV) stage or after maturation in vitro (IVM). Their viability was assessed by in vitro fertilization (IVF) and culture (IVC...

journal_title：Molecular reproduction and development

authors： Fuku EJ,Liu J,Downey BR

更新日期：1995-02-01 00:00:00

abstract：:The presence and possible role of products of nuclear (c-fos and c-jun) and c-ras proto-oncogenes were investigated in preimplantation embryonic development in mice. Polyclonal antibodies to c-fos or c-jun proto-oncogene products did not affect development of in vitro-cultured embryos from two-cell to morula or from m...

journal_title：Molecular reproduction and development

authors： Ahmad K,Naz RK

更新日期：1993-11-01 00:00:00

abstract：:Gonadotropin-Releasing Hormones (GnRHs) are decapeptides well known to regulate the reproductive cycle. They are expressed not only in the brain, but also in other tissues including the gonads. It is believed that they may be involved in the endocrine and paracrine regulation of the reproductive cycle. To date, two fo...

journal_title：Molecular reproduction and development

authors： Uzbekova S,Lareyre JJ,Madigou T,Davail B,Jalabert B,Breton B

更新日期：2002-05-01 00:00:00

abstract：:Seminiferous tubules of the testis and epididymal tubules in adult rodents are enveloped by contractile myoid cells, which move sperm and fluids along the male reproductive tract. Myoid cells in the testis influence Sertoli cells by paracrine signaling, but their role in the epididymis is unknown. Electron microscopy ...

journal_title：Molecular reproduction and development

authors： Oliveira RL,Parent A,Cyr DG,Gregory M,Mandato CA,Smith CE,Hermo L

更新日期：2016-06-01 00:00:00

abstract：:Embryonal carcinoma (EC) cells are used widely as a model system for studying the expression of developmentally regulated genes, in particular genes that are regulated at the transcriptional level when EC cells differentiate. This review focuses on the molecular mechanisms that govern the transcription of the fibrobla...

journal_title：Molecular reproduction and development

authors： Lamb KA,Rizzino A

更新日期：1998-10-01 00:00:00

abstract：:XIST is an X-linked, non-coding gene responsible for the cis induction of X-chromosome inactivation (XCI). Knockout of the XIST allele on an active X chromosome abolishes erroneous XCI and enhances the in vivo development of cloned mouse embryos by more than 10-fold. This study aimed to investigate whether a similar m...

journal_title：Molecular reproduction and development

authors： Yang Y,Wu D,Liu D,Shi J,Zhou R,He X,Quan J,Cai G,Zheng E,Wu Z,Li Z

更新日期：2017-06-01 00:00:00

abstract：:Various thiol compounds are known to improve cytoplasmic and/or nuclear maturation of oocytes in vitro. The present study examined the effects of two thiol compounds, cysteine (0.1, 0.5, and 1.0 mM) and cysteamine (50, 100, and 200 microM), on cytoplasmic and nuclear maturation of canine oocytes. Oocytes collected fro...

journal_title：Molecular reproduction and development

authors： Hossein MS,Kim MK,Jang G,Oh HJ,Koo O,Kim JJ,Kang SK,Lee BC,Hwang WS

更新日期：2007-09-01 00:00:00

abstract：:Enzyme activities were determined quantitatively in individual rat oocytes to study their energy metabolism during maturation. Low hexokinase activity and high activities of lactate dehydrogenase and enzymes in the phosphate pathway, i.e., glucose 6-P and 6-P gluconate dehydrogenases, were characteristic of immature o...

journal_title：Molecular reproduction and development

authors： Tsutsumi O,Satoh K,Taketani Y,Kato T

更新日期：1992-11-01 00:00:00

abstract：:Normal epididymal function, such as protein expression and secretion, is primarily regulated by testicular androgens and temperature. However, the role of spermatozoa in this critical process has never been studied. In order to determine whether sperm itself could regulate epididymal function, we have developed a cell...

journal_title：Molecular reproduction and development

authors： Reyes-Moreno C,Laflamme J,Frenette G,Sirard MA,Sullivan R

更新日期：2008-03-01 00:00:00

abstract：:RNase MRP is a ribonucleoprotein endoribonuclease found predominantly in nucleoli, but which has been associated with mitochondria and mitochondrial RNA processing. In order to analyze the intracellular localization of specific RNA components of ribonucleoproteins of this type, a whole-mount method for in situ hybridi...

journal_title：Molecular reproduction and development

authors： Davis AF,Jeong-Yu S,Clayton DA

更新日期：1995-11-01 00:00:00

abstract：:The present study was conducted to reveal effects of in ovo injection of nonsteroidal aromatase inhibitor (Fadrozole) or estradiol at day 3 of incubation on mRNA levels of P45017alphahydroxylase (P450c17), P450 aromatase (P450arom) and anti-Müllerian hormone (AMH) in the chicken gonads. The mRNA levels in the gonads a...

journal_title：Molecular reproduction and development

authors： Nishikimi H,Kansaku N,Saito N,Usami M,Ohno Y,Shimada K

更新日期：2000-01-01 00:00:00

abstract：:Cysteamine when added during in vitro maturation (IVM) or in vitro embryo culture (IVC) stimulates glutathione (GSH) synthesis and improves embryo developmental rates. This suggests that GSH synthesis is decreased in the in vitro produced embryo. The present study was carried out to evaluate if addition of cysteamine ...

journal_title：Molecular reproduction and development

authors： de Matos DG,Herrera C,Cortvrindt R,Smitz J,Van Soom A,Nogueira D,Pasqualini RS

更新日期：2002-06-01 00:00:00

abstract：:To improve in vitro maturation and to understand the mechanism for meiotic resumption of oocytes, meiotic progression, and its control by hypoxanthine (HX) were studied in goat oocytes. Ovaries were obtained from a local abattoir, and cumulus-oocyte complexes (COCs) and follicular fluid were collected from follicles o...

journal_title：Molecular reproduction and development

authors： Ma S,Lan G,Miao Y,Wang Z,Chang Z,Luo M,Tan J

更新日期：2003-11-01 00:00:00

abstract：:Estrogen receptor mRNA expression was detected in the head and tail parts of the human epididymis by means of reverse transcription polymerase chain reaction (RT-PCR). Immunocytochemical labelling showed that ciliate and nonciliate cells from the epithelium of the efferent ductules possessed strong to moderate nuclear...

journal_title：Molecular reproduction and development

authors： Ergün S,Ungefroren H,Holstein AF,Davidoff MS

更新日期：1997-08-01 00:00:00

abstract：:Sperm capacitation is defined as the maturational changes that render a sperm competent for fertilization and occurs in the female reproductive tract. Identification of the factor/s that regulate sperm capacitation would allow the understanding of these phenomena. Among these factors, gamma-aminobutyric acid (GABA) ha...

journal_title：Molecular reproduction and development

authors： Ritta MN,Bas DE,Tartaglione CM

更新日期：2004-04-01 00:00:00