Abstract:
OBJECTIVE:To compare polyacrylamide gel as synthetic medium with human cervical mucus (CM) for the in vitro sperm-penetration test during infertility investigation. PATIENTS:One hundred sixty-nine randomly chosen couples with a median duration of infertility of 4 (range, 1 to 16) years presenting at the infertility unit of the Women's University Hospital of Heidelberg, Germany. MAIN OUTCOME MEASURES:Evaluation of sperm migration in polyacrylamide gel used in four different concentrations (1.5%, 1.6%, 1.7%, 1.8%) in the capillary tube test in parallel with CM of patients' female partners and CM of fertile donors, obtained under standardized conditions. Correlation of migration test results with outcome of semen analysis including microbial cultures and testing for local antisperm antibodies by means of the mixed antiglobulin reaction, postcoital testing, and the subsequent pregnancy rate after control for female infertility factors in a prospective study. RESULTS:Sperm ability to penetrate the synthetic medium (concerning all concentrations) correlated significantly with the penetration of human CM, although polyacrylamide proved to be a stronger barrier. Sperm velocity and duration of progressive motility were markedly reduced in polyacrylamide. Polyacrylamide results correlated with the outcome of standard sperm analyses but not with sperm antibody testing. No clear differentiation was obtained with regard to subsequent fertility (19% after 6 months), although adequate sperm migration in polyacrylamide 1.8% was significantly more frequent in the fertile group. CONCLUSIONS:In analyzing the intrinsic motility, penetration testing with polyacrylamide gel provides important information not obtained by routine sperm analysis. However, particularly with regard to immunological factors and fertility prognosis, human CM should be preferred whenever possible.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Eggert-Kruse W,Schwalbach B,Rohr G,Klinga K,Tilgen W,Runnebaum Bdoi
10.1016/s0015-0282(16)56174-3subject
Has Abstractpub_date
1993-09-01 00:00:00pages
540-9issue
3eissn
0015-0282issn
1556-5653pii
S0015-0282(16)56174-3journal_volume
60pub_type
临床试验,杂志文章abstract::Transvaginal follicular aspiration (TVA) with ultrasonically guided needles allows the transfer of in vitro generated embryos to the fallopian tubes (TET), performing only one surgical procedure in the process. Up to now, this approach has been used to treat 16 couples with infertility due to severe male factor. Folli...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)60136-x
更新日期:1988-09-01 00:00:00
abstract:OBJECTIVE:To measure free T, free E2, and sex hormone-binding globulin (SHBG) levels in oligozoospermic men with varicoceles. DESIGN:Retrospective study. PATIENTS:Sixty-four infertile patients with varicoceles. SETTING:A university-based tertiary care referral center. INTERVENTIONS:Assessment of seminal cellular ch...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:
更新日期:1996-08-01 00:00:00
abstract:OBJECTIVE:To study and compare the effects of three sperm separation media, two silica-based (Percoll; Pharmacia Biotech AB, Uppsala, Sweden, and Isolate; Irvine Scientific, Santa Ana, CA) and one non-silica-based (Ixaprep; Medicult, Copenhagen, Denmark), on the recovery of progressive motile sperm, the percentage of s...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(99)00375-1
更新日期:1999-11-01 00:00:00
abstract::Meta-analyses have become an increasingly popular method of drawing conclusions when there are multiple publications addressing a particular topic. While the statistical calculations are straightforward, many of the decisions regarding the research question, populations of interest, and inclusion/exclusion criteria ar...
journal_title:Fertility and sterility
pub_type: 杂志文章,评审
doi:10.1016/j.fertnstert.2011.05.029
更新日期:2011-07-01 00:00:00
abstract::In an attempt to improve the motility of thawed spermatozoa after 1 week of its being frozen, we treated ten normal semen samples with 16.7 nmol relaxin and 6 mmol caffeine. Motility and grade of forward progression were examined after thawing. Caffeine significantly improved motility of spermatozoa. Relaxin, when add...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)48868-0
更新日期:1985-09-01 00:00:00
abstract:OBJECTIVE:To evaluate the effect of metformin in polycystic ovary syndrome (PCOS). As follow-up on a previous paper describing hormonal and metabolic factors, this paper focuses on correlations between adiponectin and anthropometric, hormonal, and metabolic factors in PCOS and the effect of metformin. DESIGN:Randomize...
journal_title:Fertility and sterility
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.fertnstert.2010.01.057
更新日期:2010-11-01 00:00:00
abstract:OBJECTIVE:To investigate P secretion by granulosa cells (GCs) versus cumulus cells derived from human preovulatory follicles. DESIGN:Cells were recovered by aspiration of preovulatory follicles in 44 women participating in an IVF program. Induction of ovulation was performed using clomiphene citrate, hMG and hCG (grou...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)56404-8
更新日期:1993-12-01 00:00:00
abstract:OBJECTIVE:To determine the fertility potential of women with congenital ampullary atresia of the fallopian tube. DESIGN:Case report and review of literature. SETTING:University-affiliated tertiary care infertility clinic. PATIENT(S):Six infertile women with congenital ampullary atresia of the oviduct. INTERVENTION(...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(02)04691-5
更新日期:2003-02-01 00:00:00
abstract:OBJECTIVE:To evaluate and compare transforming growth factor β3 (TGF-β3) serum concentration in patients with uterine fibroids (UFs) without hormone treatment, treated with ulipristal acetate (UPA), and controls; to evaluate TGF-β3 concentrations in UF tissue in patients without hormone treatment and those treated with...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2017.11.023
更新日期:2018-03-01 00:00:00
abstract:OBJECTIVES:To examine and discuss the pathology, diagnosis, incidence, and patient profile of salpingitis isthmica nodosa and to question its natural history, propose management strategies, and identify areas of promising research. DESIGN:Over 50 studies were reviewed, evaluated, and compared to offer the clinician a ...
journal_title:Fertility and sterility
pub_type: 杂志文章,评审
doi:10.1016/s0015-0282(16)56207-4
更新日期:1993-10-01 00:00:00
abstract:OBJECTIVE:To conduct a pilot study to investigate the possible presence of bacteria throughout the female reproductive tract and to make a preliminary assessment of whether there are differences in the composition of the microbial communities between these body sites and/or between patients. DESIGN:Prospective pilot s...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2016.11.028
更新日期:2017-03-01 00:00:00
abstract::On the basis of studies in monkeys, the hypothesis has been proposed that vasectomy induces the formation of circulating immune complexes (CICs), which--via activation of the complement system--may result in immune injury of the endothelium and thereby exacerbate atherosclerosis; the same mechanism has been suggested ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:
更新日期:1982-06-01 00:00:00
abstract::The penetration of a gonadotropin-releasing hormone agonist (GnRH-a), Buserelin (Hoechst AG, Frankfurt, West Germany), into human follicular fluids (FF) was studied by means of a radioimmunoassay (RIA) and a bioassay. Acute nasal administration of a therapeutic dose of Buserelin (300 and 600 micrograms) before the ovu...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)60852-x
更新日期:1989-08-01 00:00:00
abstract::Eight castrate, estrogen-replaced women were given 200 mg spironolactone daily for 4 weeks. The response of plasma dehydroepiandrosterone sulfate (DHEAS), testosterone (T), and androstenedione (delta 4A), all indicators of adrenal C19-steroid production, varied greatly among individuals. Sixteen women with idiopathic ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)59346-7
更新日期:1987-08-01 00:00:00
abstract::The beta-adrenergic agonist isoproterenol was infused intravenously for 4 hours during the midluteal phase of the cycle in six normal women. A rapid increase in cardiac output occurred for the entire duration of the isoproterenol infusion and serum estrogen and progesterone levels (but not luteinizing hormone levels) ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)47712-5
更新日期:1984-03-01 00:00:00
abstract:OBJECTIVE:To develop an understanding of hypogonadal men with a history of anabolic-androgenic steroid (AAS) use and to outline recommendations for management. DESIGN:Review of published literature and expert opinions. Intended as a meta-analysis, but no quality studies met the inclusion criteria. SETTING:Not applica...
journal_title:Fertility and sterility
pub_type: 杂志文章,meta分析,评审
doi:10.1016/j.fertnstert.2014.02.002
更新日期:2014-05-01 00:00:00
abstract:OBJECTIVE:To evaluate, using the hemizona assay (HZA), whether egg yolk treatment of human sperm enhances binding to the human zona pellucida in vitro and to determine whether such a treatment is as efficient as the standard swim-up procedure for promoting sperm binding ability. DESIGN:Ejaculates were divided into ali...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)55261-3
更新日期:1992-09-01 00:00:00
abstract::This study describes the results with immature human follicular oocytes harvested from unstimulated ovaries, matured in vitro, fertilized, and transferred to an agonadal recipient. Two hundred seventy immature oocytes were aspirated from 23 ovaries removed for various gynecological indications from August 1988 to Octo...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)54068-0
更新日期:1991-01-01 00:00:00
abstract::This retrospective study examined the association of endometrial pattern and pregnancy after a day 5 ET. The pregnancy rate of women with a triple-line ultrasound endometrial pattern on the day of hCG administration was significantly higher than the pregnancy rate of women with the other ultrasound patterns. This obse...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2004.05.102
更新日期:2005-01-01 00:00:00
abstract::The immunobead test was used to study the immunoglobulin class of antibodies on sperm before and after penetration through a microcolumn of cervical mucus. Sixteen men with positive sperm antibodies (positive sperm immobilization test) and sperm that penetrated cervical mucus in prior tests were selected for study. Ho...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)48916-8
更新日期:1985-10-01 00:00:00
abstract:OBJECTIVE:To analyze the endometrium sonographically in patients undergoing controlled ovarian stimulation with clomiphene citrate (CC) in addition to menotropins to determine the significance of endometrial pattern and thickness on pregnancy rate (PR). DESIGN:A prospective study analyzing patients receiving sequentia...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(97)81509-9
更新日期:1997-08-01 00:00:00
abstract::Fertilization, embryo development, and successful pregnancy can be achieved after transfer of embryos derived from brown oocytes at the same rate as embryos from morphologically normal oocytes. Therefore, brown oocytes are probably normal and this morphological criterion does not seem to be indicative of any adverse o...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2006.03.056
更新日期:2006-11-01 00:00:00
abstract:OBJECTIVE:To report two cases of early onset cholestasis of pregnancy associated with IVF and ovarian hyperstimulation syndrome. DESIGN:Case report. SETTING:University-based IVF program. PATIENT(S):Two patients with first-trimester cholestasis of pregnancy after IVF that was associated with ovarian hyperstimulation ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2007.08.072
更新日期:2008-10-01 00:00:00
abstract::Although previous large series have reported that from 0.3% to 1.3% of patients undergoing hysterosalpingography (HSG) develop infectious morbidity, our impression was that the incidence was higher among our patients. Therefore the records of 448 consecutive healthy women who underwent HSG were examined. Within 24 hou...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)44712-6
更新日期:1980-05-01 00:00:00
abstract::Preimplantation genetic diagnosis has been proposed as a method to improve assisted reproduction technology outcomes, but different techniques have produced conflicting results. The use of appropriate techniques may provide positive outcomes. ...
journal_title:Fertility and sterility
pub_type: 杂志文章,评审
doi:10.1016/j.fertnstert.2009.02.091
更新日期:2010-07-01 00:00:00
abstract::To assess whether procedures of semen preparation can reduce the proportion of antibody-coated spermatozoa, semen samples with positive direct mixed antiglobulin reaction (MAR) were washed in media supplemented with 10% or 50% fetal cord serum (FCS). Washing reduced the MAR to a negative level, but the MAR was identic...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)53169-0
更新日期:1989-12-01 00:00:00
abstract:OBJECTIVE:To investigate whether antitrophoblast antibodies are associated with unexplained recurrent miscarriages, we used choriocarcinoma cells JEG-3, since these cells are negative for class I and II antigens, but they do express HLA-G, resembling an antigen expression of endovascular and interstitial trophoblasts. ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2011.11.014
更新日期:2012-02-01 00:00:00
abstract:OBJECTIVE:To investigate the molecular mechanism for declining sperm motility in older men. DESIGN:Retrospective and prospective study. SETTING:University-based andrology unit. PATIENT(S):Semen was collected from 2,111 patients and sperm donors. INTERVENTION(S):None. MAIN OUTCOME MEASURE(S):Age, sperm concentratio...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2005.05.020
更新日期:2005-11-01 00:00:00
abstract::The effects of freezing and thawing (F/T) on functional activity of immature rat oocyte-cumulus complexes (OCC) were studied. The OCC were divided into three groups according to the number of cumulus-cell layers surrounding them. The OCC were then frozen and thawed (F/T), with dimethyl sulfoxide (DMSO) as cryoprotecta...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:
更新日期:1988-11-01 00:00:00
abstract::Seven nonionic detergents of the Igepal CO series differing in molecular size and including nonoxynol-9 and a representative anionic (SDS) and cationic (Cepacol) detergent were tested as to their relative cytotoxicity. The biologic effects of these detergents on human W1--38 fibroblasts (DNA and glycosaminoglycans syn...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:
更新日期:1980-05-01 00:00:00