Abstract:
:This study describes the results with immature human follicular oocytes harvested from unstimulated ovaries, matured in vitro, fertilized, and transferred to an agonadal recipient. Two hundred seventy immature oocytes were aspirated from 23 ovaries removed for various gynecological indications from August 1988 to October 1989. The numbers of follicular oocytes collected from ovaries were compared by patients' ages and the stages of menstrual cycle. Immature oocytes in vitro were incubated with either mature follicular fluid (FF) or fetal cord serum (FCS). The maturation rate in the mature FF group was 55.8%, significantly higher than the 35.9% in the FCS group. In addition, mature FF group was shown to provide a significantly higher fertilization rate than the FCS group (81.0% versus 31.6%). More fertilized eggs developed into normal embryos in the nonstimulated cycle group than in stimulated cycles with routine treatment. Finally, five embryos were transferred to a woman with premature ovarian failure on day 18 of a steroid replacement cycle. She subsequently delivered healthy triplet girls. These results suggest that in vitro maturation of immature oocytes from unstimulated ovaries with mature follicular fluid could be used successfully in a donor oocyte program after in vitro fertilization.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Cha KY,Koo JJ,Ko JJ,Choi DH,Han SY,Yoon TKdoi
10.1016/s0015-0282(16)54068-0subject
Has Abstractpub_date
1991-01-01 00:00:00pages
109-13issue
1eissn
0015-0282issn
1556-5653pii
S0015-0282(16)54068-0journal_volume
55pub_type
杂志文章abstract:OBJECTIVE:To characterize and to purify the growth substances for mouse embryo fibroblasts (NIH/3T3) and endometrial-like cells of the peritoneal fluid of women with endometriosis. DESIGN:A pool of 102 mL of peritoneal fluid (PF) collected from 24 women with laparoscopic evidence of endometriosis was dialyzed and ther...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
abstract::We studied a prospective cohort of 434 couples in Northern California and found that 13% did not pursue any form of infertility treatment after their initial consultation. Although age, education, and financial concerns remain important for patients in choosing whether to pursue infertility treatment, depressive sympt...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2010.03.068
更新日期:2010-11-01 00:00:00
abstract::Nonaromatizable androgens, administered in high doses to an adult patient with partial androgen insensitivity, failed to result in a change in phallic size despite a clear decline in SHBG and gonadotropin levels. These findings raise the question of differential tissue sensitivity to androgens. Because ancillary labor...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)56114-7
更新日期:1993-08-01 00:00:00
abstract::To understand the mechanism of action of danazol, the binding of danazol to multiple classes of intracellular steroid binding proteins was studied in the human uterine endometrium. Danazol bound to endometrial receptors for estrogen, progesterone, and androgen and seemed to bind to endometrial intracellular corticoste...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)47840-4
更新日期:1984-05-01 00:00:00
abstract::The immune system plays a critical role in pregnancy establishment and maintenance, but application of immunological principles to the treatment of infertility and pregnancy loss has had a checkered past. Improving knowledge in this area holds great promise for improving the treatment of subfertile patients. ...
journal_title:Fertility and sterility
pub_type:
doi:10.1016/j.fertnstert.2016.07.1090
更新日期:2016-09-01 00:00:00
abstract:OBJECTIVE:To compare two commonly used protocols (fresh vs. vitrified) used to transfer euploid blastocysts after IVF with preimplantation genetic screening. DESIGN:Randomized controlled trial. SETTING:Private assisted reproduction center. PATIENT(S):A total of 179 patients undergoing IVF treatment using preimplanta...
journal_title:Fertility and sterility
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.fertnstert.2016.12.022
更新日期:2017-03-01 00:00:00
abstract:OBJECTIVE:To improve the maturation rate of murine and human germinal vesicle (GV) oocytes using human tubal epithelial cells (hTECs). DESIGN:Murine and human GV oocytes were randomized to human tubal fluid (HTF) media alone or cocultured with mouse embryonic fibroblasts (MEFs) or primary hTECs or immortalized hTECS (...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2010.08.034
更新日期:2011-03-15 00:00:00
abstract:OBJECTIVE:To report preterm birth and small for gestational age (SGA) rates from assisted reproduction technologies (ART) patients with ovarian endometriomata compared with control groups. DESIGN:Retrospective cohort study. SETTING:Tertiary university affiliated ART center and Perinatal Data Collection Unit (PDCU). ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2008.01.096
更新日期:2009-02-01 00:00:00
abstract::Two successive intranasal doses (300 micrograms at 8:00 A.M. and 6:00 P.M.) of the luteinizing hormone-releasing hormone (LH-RH) agonist (D-Ser[TBU]6-des-Gly-NH2(10))LH-RH ethylamide (Buserelin) were administered on days 1 to 10 after the midcycle luteinizing hormone (LH) surge in 33 normally cycling women. Maximal st...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)47062-7
更新日期:1983-05-01 00:00:00
abstract:OBJECTIVE:To validate recently proposed models of ovarian nongrowing follicle (NGF) decay associated with aging within the context of an independent data set. DESIGN:Prospective investigation. SETTING:Academic medical center. PATIENT(S):Normal appearing ovaries collected from 52 women (age 28-51 years) undergoing oo...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2013.12.008
更新日期:2014-03-01 00:00:00
abstract::Discrepancies about a possible relationship between prothrombotic mutations and a decreased uteroplacental blood flow are present in literature. We prospectively evaluated a cohort of pregnant women (n = 41) with bilateral uterine artery notches and found that women with inherited thrombophilia showed a sixfold higher...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2006.01.038
更新日期:2006-09-01 00:00:00
abstract:OBJECTIVE:To determine if the solubilized human zona pellucida (ZP)-induced acrosome reaction is mediated by protein kinases. DESIGN:Capacitated spermatozoa were incubated with inhibitors of cyclic adenosine 3':5'-monophosphate (cAMP)-dependent kinase (KT5720), Ca(2+)- and phospholipid-dependent kinase (Calphostin C),...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:
更新日期:1994-03-01 00:00:00
abstract::To explore possible relationships between genital tract infections, sperm antibodies, and infertility, seminal fluid from 100 men was analyzed. Sperm antibodies, measured by an enzyme-linked immunosorbent assay, were detected in 14 of 29 men (48%) with culture-positive asymptomatic infections, 8 of 17 men (47%) with a...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)47484-4
更新日期:1983-12-01 00:00:00
abstract:OBJECTIVE:To determine the effect of human sperm incubation at room temperature (20 degrees C) upon capacitation-related events. DESIGN:Prospective study. SETTING:Basic research laboratory. PATIENT(S):Semen samples were obtained from normozoospermic volunteers. Human follicular fluid (hFF) was collected from women u...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(01)02982-x
更新日期:2002-02-01 00:00:00
abstract:OBJECTIVE:To determine the proportion of euthyroid women attending a fertility practice who develop hypothyroidism in very early pregnancy (gestational hypothyroidism [GHT]), and to examine the association of GHT with exogenous gonadotropin treatment. DESIGN:Retrospective cohort study. SETTING:A private reproductive ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2015.03.006
更新日期:2015-06-01 00:00:00
abstract::The promoter of P receptor isoform B (PR-B) has recently been shown to be hypermethylated in endometriotic tissues, but one question remains as what causes the aberrant methylation. We show that sustained stimulation of an epithelial-like endometriotic cell line with tumor necrosis factor alpha stimulation for 30 days...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2007.06.008
更新日期:2008-07-01 00:00:00
abstract:OBJECTIVE:To assign cutoff values for human chorionic gonadotropin (beta-hCG) in pretreatment and after one methotrexate (MTX) cycle and determine its correspondence to the number of MTX cycles in successfully treated ectopic pregnancy. DESIGN:Retrospective study. SETTING:Polish university hospital. PATIENT(S):68 wo...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2008.07.1775
更新日期:2009-10-01 00:00:00
abstract:OBJECTIVE:To determine Müllerian inhibiting substance (MIS) levels in follicular fluid (FF) and sera of IVF patients. DESIGN:Prospective study. SETTING:Fertility center. PATIENT(S):Sixty-six patients: 20 with tubal factor infertility, 17 with polycystic ovary syndrome (PCOS), and 29 with endometriosis. INTERVENTION...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(97)81417-3
更新日期:1997-05-01 00:00:00
abstract:OBJECTIVE:To assess the safety and effectiveness of a sprayable, site-specific adhesion barrier system (SprayGel; Confluent Surgical, Waltham, MA) for reduction of adhesion formation. DESIGN:Prospective, randomized, controlled phase III trial. SETTING:University clinic (Kiel, Germany) and private clinic (Bordeaux, Fr...
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1016/j.fertnstert.2003.12.046
更新日期:2004-08-01 00:00:00
abstract::Throughout the world, adults with HIV are living longer, and many are assessing their options for reproduction. The growing body of scientific evidence and commentary concerning the outcomes of infertility services provided to these adults demands systematic summary and long-term surveillance if safety, quality, and b...
journal_title:Fertility and sterility
pub_type: 评论,杂志文章
doi:10.1016/j.fertnstert.2005.07.1325
更新日期:2006-02-01 00:00:00
abstract::Preimplantation genetic diagnosis has been proposed as a method to improve assisted reproduction technology outcomes, but different techniques have produced conflicting results. The use of appropriate techniques may provide positive outcomes. ...
journal_title:Fertility and sterility
pub_type: 杂志文章,评审
doi:10.1016/j.fertnstert.2009.02.091
更新日期:2010-07-01 00:00:00
abstract:OBJECTIVE:To determine the prevalence of anticardiolipin and antiphophatidylserine antibodies in an IVF population and to correlate their presence and specific isotype with IVF cycle outcome. DESIGN:Retrospective clinical study using stored midfollicular sera for determination of antibody status. SETTING:University h...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(97)81519-1
更新日期:1997-08-01 00:00:00
abstract::Five patients with primary infertility and secondary amenorrhea who did not respond to clomiphene with a gonadotropin or estrogen surge were treated with 500 mug of luteinizing hormone, follicle-stimulating hormone-releasing hormone (LH-RH), self-administered subcutaneously every 8 hours for 14 days. Of four patients ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)41890-x
更新日期:1976-06-01 00:00:00
abstract:OBJECTIVE:To review the effectiveness of rectal probe electroejaculation in terms of pregnancy in treatment of anejaculatory males due to spinal cord injury. The characteristics of electroejaculates also are examined. DATA IDENTIFICATION:All English language studies reporting pregnancies as a result of electroejaculat...
journal_title:Fertility and sterility
pub_type: 杂志文章,评审
doi:
更新日期:1995-07-01 00:00:00
abstract:OBJECTIVE:To assess the molecular signatures underlying endometrial disorder using cDNA microarray. DESIGN:Gene expression-based oligonucleotide array of the normal endometrium. SETTING:University hospital. PATIENT(S):Humans. INTERVENTION(S):Endometrial tissues were obtained from 28 normal cycling women undergoing ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2009.01.130
更新日期:2010-06-01 00:00:00
abstract:OBJECTIVE:To investigate the influence of folic acid supplementation on the follicular fluid concentrations of folate and total homocysteine and their relationship to the diameter of the follicle. DESIGN:Observational study. SETTING:Tertiary referral fertility clinic at the Erasmus MC, University Medical Center, Rott...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2007.06.036
更新日期:2008-06-01 00:00:00
abstract:OBJECTIVE:To investigate the effect of human oviductal cells on various sperm functions in vitro. DESIGN:Controlled experimental laboratory study. SETTING:University gynecology unit. PATIENT(S):Women undergoing tubal ligation or hysterectomy and men who were visiting our subfertility clinics. INTERVENTION(S):Cocult...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(98)00430-0
更新日期:1999-02-01 00:00:00
abstract:OBJECTIVE:To evaluate the role of the environmental estrogens polychlorinated biphenyls (PCBs) and phthalate esters (PEs) as potential environmental hazards in the deterioration of semen parameters in infertile men without an obvious etiology. DESIGN:Randomized controlled study. SETTING:Tertiary care referral inferti...
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/s0015-0282(02)04389-3
更新日期:2002-12-01 00:00:00
abstract:OBJECTIVE:To explore the role of tumor necrosis factor (TNF) α, an early embryonic product, on endometrial epithelial cell migration and endometrial milk fat globule-epidermal growth factor 8 protein (MFG-E8) production. DESIGN:In vitro study. SETTING:Academic center. INTERVENTION(S):Ishikawa cells, used as surrogat...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2013.10.032
更新日期:2014-02-01 00:00:00
abstract::Selenium (Se) was measured in the semen of 125 men from couples consulting for infertility. A mean concentration of 71.3 +/- 29.7 ng/ml of semen was found, with a range of 7 to 230 ng/ml. More than 85% of the Se is in the seminal plasma. There was a significant positive correlation between sperm count and semen Se. Sp...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:
更新日期:1984-12-01 00:00:00