当前位置： SCI文献检索 > FERTILITY AND STERILITY期刊下所有文献 > In vitro maturation (IVM) of murine and human germinal vesicle (GV)-stage oocytes by coculture with immortalized human fallopian tube epithelial cells.

In vitro maturation (IVM) of murine and human germinal vesicle (GV)-stage oocytes by coculture with immortalized human fallopian tube epithelial cells.

Abstract：

OBJECTIVE:To improve the maturation rate of murine and human germinal vesicle (GV) oocytes using human tubal epithelial cells (hTECs). DESIGN:Murine and human GV oocytes were randomized to human tubal fluid (HTF) media alone or cocultured with mouse embryonic fibroblasts (MEFs) or primary hTECs or immortalized hTECS (ihTECs) for various times. Rates of maturation to meiosis II (MII) were compared between groups. INTERVENTION(S):TECs were isolated from discarded salpingectomy specimens. One batch was immortalized with TERT and SV40 large T-antigen. GV oocytes (n = 710) were isolated from 8-week-old-mice at 40 hours after pregnant mare's serum gonadotropin stimulation. Discarded human GV oocytes (n = 62) were obtained from intracytoplasmic sperm injection cycle IVF center patients. Oocytes were cultured in HTF media alone or with MEFs, hTECs, or ihTECs. MAIN OUTCOME MEASURE(S):Maturation rates were assessed by standard morphological criteria and compared. RESULT(S):The maturation rate of murine GV oocytes to MII at 12 and 24 hours increased significantly in coculture with hTECS and ihTECS compared with MEF and HTF media alone. In addition, the development rate after IVF was significantly higher with hTECS and ihTECS than in MEF and HTF media alone. Maturation of human GV oocytes to MII at 24 and 48 hours was significantly higher in hTECS and ihTECS compared with HTF media alone. CONCLUSION(S):Coculture with either primary or immortalized TECs might improve oocyte quality and significantly raise in vitro maturation rates for GV oocytes.

journal_name

Fertil Steril

journal_title

Fertility and sterility

authors

Lee HJ,Quaas AM,Wright DL,Toth TL,Teixeira JM

doi

10.1016/j.fertnstert.2010.08.034

subject

Has Abstract

pub_date

2011-03-15 00:00:00

pages

1344-8

issue

4

eissn

0015-0282

issn

1556-5653

pii

S0015-0282(10)02422-2

journal_volume

95

pub_type

杂志文章

相关文献

FERTILITY AND STERILITY文献大全
  • Absence of block to polyspermy at the human oolemma.

    abstract：OBJECTIVE:To study the fusiogenic ability of human spermatozoa and oocytes. DESIGN:Retrospective study of 3,027 oocytes included in a program of subzonal insemination (SUZI). SETTING:Assisted fertilization program in an academic research environment. PATIENT(S):Couples with characterized male factor infertility or p...

    journal_title：Fertility and sterility

    pub_type： 杂志文章

    doi：10.1016/s0015-0282(97)81445-8

    authors： Wolf JP,Ducot B,Aymar C,Rodrigues D,Desjardin S,Jardin A,Jouannet P

    更新日期：1997-06-01 00:00:00

  • Studies on nonoxynol-9. III. Effect on fibroblasts and spermatozoa.

    abstract：:Seven nonionic detergents of the Igepal CO series differing in molecular size and including nonoxynol-9 and a representative anionic (SDS) and cationic (Cepacol) detergent were tested as to their relative cytotoxicity. The biologic effects of these detergents on human W1--38 fibroblasts (DNA and glycosaminoglycans syn...

    journal_title：Fertility and sterility

    pub_type： 杂志文章

    doi：

    authors： Chvapil M,Ulreich JB,O'Dea K,Betts K,Droegemueller W

    更新日期：1980-05-01 00:00:00

  • Essure hysteroscopic tubal occlusion device for the treatment of hydrosalpinx prior to in vitro fertilization-embryo transfer in patients with a contraindication for laparoscopy.

    abstract：OBJECTIVE:To investigate the success rate of proximal tubal occlusion with Essure devices in subfertile women with hydrosalpinges, and to observe the results of subsequent treatment with IVF. DESIGN:Prospective, single-arm, clinical study. SETTING:University hospital and teaching hospital. PATIENT(S):Ten women with ...

    journal_title：Fertility and sterility

    pub_type： 临床试验,杂志文章

    doi：10.1016/j.fertnstert.2008.11.022

    authors： Mijatovic V,Veersema S,Emanuel MH,Schats R,Hompes PG

    更新日期：2010-03-01 00:00:00

  • On the morphology of live spermatozoa of human semen.

    abstract：:Live and dead spermatozoa of human semen were distinguished and evaluated separately with respect to their morphology after supravital staining with buffered eosin. This evaluation was shown to be almost as reliable as that of the commonly stained smear. Different samples from the same individual were found to be simi...

    journal_title：Fertility and sterility

    pub_type： 杂志文章

    doi：10.1016/s0015-0282(16)42377-0

    authors： Fredricsson B,Waxegård G,Brege S,Lundberg I

    更新日期：1977-02-01 00:00:00

  • Undetectable serum anti-Müllerian hormone levels and occurrence of ongoing pregnancy.

    abstract：OBJECTIVE:To discuss, on the basis of the experience of two clinical cases and extensive literature review, the significance of extremely low levels of anti-Müllerian hormone (AMH), also known as Müllerian-inhibiting substance, in infertile women. DESIGN:Case report. SETTING:University-based infertility clinic at a m...

    journal_title：Fertility and sterility

    pub_type： 杂志文章,评审

    doi：10.1016/j.fertnstert.2007.03.084

    authors： Fraisse T,Ibecheole V,Streuli I,Bischof P,de Ziegler D

    更新日期：2008-03-01 00:00:00

  • Significance of inhibin B and anti-Müllerian hormone in seminal plasma: a preliminary study.

    abstract：OBJECTIVE:To evaluate the predictive value of seminal inhibin B and anti-Müllerian hormone (AMH) on the outcome of testicular sperm extraction (TESE) in patients with nonobstructive azoospermia. DESIGN:Prospective study. SETTING:Reproductive biology department. PATIENT(S):Forty-seven normospermic, 28 oligozoospermic...

    journal_title：Fertility and sterility

    pub_type： 杂志文章

    doi：10.1016/j.fertnstert.2007.03.032

    authors： Duvilla E,Lejeune H,Trombert-Paviot B,Gentil-Perret A,Tostain J,Levy R

    更新日期：2008-02-01 00:00:00

  • Quantification of spermatogenesis by dual-parameter flow cytometry.

    abstract：OBJECTIVE:To investigate the usefulness of dual-parameter flow cytometry of testicular fine-needle aspirates for the quantification of spermatogenesis. Immunofluorescence staining for vimentin was introduced to discriminate vimentin-negative germ cells from vimentin-positive stromal cells. The results of flow cytometry...

    journal_title：Fertility and sterility

    pub_type： 杂志文章

    doi：10.1016/s0015-0282(16)56656-4

    authors： Hittmair A,Rogatsch H,Mikuz G,Feichtinger H

    更新日期：1994-04-01 00:00:00

  • Acid Tyrode's solution can stimulate parthenogenetic activation of human and mouse oocytes.

    abstract：:Fresh and aged (24 hours after ovulation) human oocytes and recently ovulated mouse oocytes may be activated by exposure to acidified Tyrode's solution. No activation of either type of human oocyte was observed after exposure to hyaluronidase or pronase, but significant numbers of fresh mouse oocytes were activated af...

    journal_title：Fertility and sterility

    pub_type： 杂志文章

    doi：10.1016/s0015-0282(16)53279-8

    authors： Johnson MH,Pickering SJ,Braude PR,Vincent C,Cant A,Currie J

    更新日期：1990-02-01 00:00:00

  • Endometrial expression of relaxin and relaxin receptor in endometriosis.

    abstract：:Our studies demonstrate significantly lower expression of relaxin and its receptor in ectopic endometriotic tissues than their expression in eutopic endometrium and in endometrium from normal controls. These data suggest that in normal and eutopic endometrium, relaxin may exert a protective effect against endometriosi...

    journal_title：Fertility and sterility

    pub_type： 杂志文章

    doi：10.1016/j.fertnstert.2010.06.051

    authors： Morelli SS,Petraglia F,Weiss G,Luisi S,Florio P,Wojtczuk A,Goldsmith LT

    更新日期：2010-12-01 00:00:00

  • Supplemented tissue culture medium 199 is a better medium for in vitro maturation of oocytes from women with polycystic ovary syndrome women than human tubal fluid.

    abstract：OBJECTIVE:To compare oocyte maturation, fertilization and cleavage rates, and embryonic developmental quality after culture of human immature oocytes from polycystic ovary syndrome (PCOS) patients in human tubal fluid (HTF) or tissue culture medium (TCM) 199. DESIGN:Prospective, randomized, controlled trial. SETTING:...

    journal_title：Fertility and sterility

    pub_type： 杂志文章,随机对照试验

    doi：10.1016/j.fertnstert.2007.11.082

    authors： de Araujo CH,Nogueira D,de Araujo MC,Martins Wde P,Ferriani RA,dos Reis RM

    更新日期：2009-02-01 00:00:00

  • Prolactin as an inhibitor of granulosa cell luteinization: implications for hyperprolactinemia-associated luteal phase dysfunction.

    abstract：:Chronic and transient hyperprolactinemia have been associated with luteal phase dysfunction in both spontaneously cycling women and those subjected to ovarian hyperstimulation by exogenous gonadotropins. It is the objective of the in vitro and in vivo studies reported herein to examine the possibility that prolactin (...

    journal_title：Fertility and sterility

    pub_type： 杂志文章

    doi：

    authors： Adashi EY,Resnick CE

    更新日期：1987-07-01 00:00:00

  • Does stimulation with human gonadotropins and gonadotropin-releasing hormone agonist enhance and accelerate the developmental capacity of oocytes in human ovarian tissue xenografted into severe combined immunodeficient mice?

    abstract：OBJECTIVE:To assess the capacity of human frozen-thawed ovarian follicles matured in xenografts to form metaphase II (MII) oocytes after xenotransplantation and exogenous stimulation. DESIGN:Prospective controlled animal study. SETTING:University hospital gynecology research unit. PATIENT(S):Ovarian fragments were o...

    journal_title：Fertility and sterility

    pub_type： 杂志文章

    doi：10.1016/j.fertnstert.2014.01.038

    authors： Lotz L,Schneider H,Hackl J,Wachter D,Hoffmann I,Jurgons R,Beckmann MW,Dittrich R

    更新日期：2014-05-01 00:00:00

  • Micromanipulation of sperm by a laser generated optical trap.

    abstract：:The force generated by the radiation pressure of a low power laser beam induces an optical trap which may be used to manipulate sperm. We studied the effect of the optical trap on sperm motility. A Nd:YAG laser beam was coupled to a conventional microscope and focused into the viewing plane by the objective lens. Sper...

    journal_title：Fertility and sterility

    pub_type： 杂志文章

    doi：

    authors： Tadir Y,Wright WH,Vafa O,Ord T,Asch RH,Berns MW

    更新日期：1989-11-01 00:00:00

  • Creation of a neovagina following Williams vaginoplasty and the Creatsas modification in 111 patients with Mayer-Rokitansky-Küster-Hauser syndrome.

    abstract：OBJECTIVE:Evaluation of the Creatsas modification of Williams vaginoplasty for the creation of neovagina in patients with Mayer-Rokitansky-Küster-Hauser syndrome (MRKH syndrome). DESIGN:Record of perioperative and postoperative results and complications. Follow-up evaluations of patients yearly after the operation. S...

    journal_title：Fertility and sterility

    pub_type： 杂志文章

    doi：10.1016/s0015-0282(01)02836-9

    authors： Creatsas G,Deligeoroglou E,Makrakis E,Kontoravdis A,Papadimitriou L

    更新日期：2001-11-01 00:00:00

  • Outcome of assisted reproductive technology in women over the age of 41.

    abstract：OBJECTIVE:To analyze the results of ongoing pregnancies and deliveries after assisted reproductive technology (ART) in women aged >/=41 years, stratified by year of age. DESIGN:Retrospective study. SETTING:University hospital, IVF unit. PATIENT(S):A total of 431 IVF and intracytoplasmic sperm injection (ICSI) cycles...

    journal_title：Fertility and sterility

    pub_type： 杂志文章

    doi：10.1016/s0015-0282(00)00697-x

    authors： Ron-El R,Raziel A,Strassburger D,Schachter M,Kasterstein E,Friedler S

    更新日期：2000-09-01 00:00:00

  • Gay men choosing parenthood through assisted reproduction: medical and psychosocial considerations.

    abstract：OBJECTIVE:To medically and psychologically assess gay men seeking parenthood through assisted reproduction and to provide guidelines for the assessment. DESIGN:Qualitative observational study. SETTING:Academic medical center. PATIENT(S):Thirty gay males (15 couples) presenting for assisted reproduction using an oocy...

    journal_title：Fertility and sterility

    pub_type： 杂志文章

    doi：10.1016/j.fertnstert.2010.05.053

    authors： Greenfeld DA,Seli E

    更新日期：2011-01-01 00:00:00

  • Fertilization and cleavage rates of heparin-exposed human oocytes in vitro, and the effect of heparin on the acrosome reaction.

    abstract：:Fertilization and cleavage rates were compared in 1024 heparin-exposed and nonexposed human oocytes recovered from 183 consecutive in vitro fertilization (IVF) cycles. Heparinized Ham's F-10 medium, (Gibco, Grand Island, NY) 1.0 ml (2.0 mg heparin/ml) was added to bloody follicular fluid; clear follicular aspirates di...

    journal_title：Fertility and sterility

    pub_type： 杂志文章

    doi：10.1016/s0015-0282(16)59476-x

    authors： Boyers SP,Tarlatzis BC,Stronk JN,DeCherney AH

    更新日期：1987-10-01 00:00:00

  • Effects of dydrogesterone and of its stable metabolite, 20-alpha-dihydrodydrogesterone, on nitric oxide synthesis in human endothelial cells.

    abstract：OBJECTIVE:To investigate the effects of P, medroxyprogesterone acetate (MPA), and dydrogesterone (DYD) and its metabolite, 20-alpha-dihydrodydrogesterone (DHD) on endothelial synthesis of nitric oxide (NO) and characterize the signaling events recruited by these compounds. The Women's Health Initiative trial reports an...

    journal_title：Fertility and sterility

    pub_type： 杂志文章

    doi：10.1016/j.fertnstert.2006.05.018

    authors： Simoncini T,Caruso A,Giretti MS,Scorticati C,Fu XD,Garibaldi S,Baldacci C,Mannella P,Fornari L,Genazzani AR

    更新日期：2006-10-01 00:00:00

  • Hyperreactio luteinalis with both markedly elevated human chorionic gonadotropin levels and an imbalance of angiogenic factors subsequently developed severe early-onset preeclampsia.

    abstract：OBJECTIVE:To report a case of hyperreactio luteinalis (HL) with elevated levels of human chorionic gonadotropin (hCG) and a severe imbalance of angiogenic factors, which developed into severe, early onset preeclampsia (PE). DESIGN:Case report. SETTING:University perinatal center. PATIENT(S):A 32-year-old primigravid...

    journal_title：Fertility and sterility

    pub_type： 杂志文章

    doi：10.1016/j.fertnstert.2009.04.002

    authors： Masuyama H,Tateishi Y,Matsuda M,Hiramatrsu Y

    更新日期：2009-07-01 00:00:00

  • Consecutive cervical pregnancies.

    abstract：OBJECTIVE:Report of a rare case of consecutive spontaneous cervical pregnancies. DESIGN:Case study. SETTING:Teaching hospital. PATIENT(S):One healthy nulliparous woman in the early years of her fourth decade. INTERVENTION(S):The first of two cervical pregnancies was treated with two doses of methotrexate and subseq...

    journal_title：Fertility and sterility

    pub_type： 杂志文章

    doi：10.1016/j.fertnstert.2003.06.012

    authors： Radpour CJ,Keenan JA

    更新日期：2004-01-01 00:00:00

  • Hysteroscopic selective salpingography.

    abstract：OBJECTIVE:To evaluate the effectiveness of hysteroscopic selective salpingography (HSS) as a method for diagnosing the tubal proximal occlusion shown by hysterosalpingography (HSG). DESIGN:Prospective study. SETTING:Outpatient Department of Obstetrics and Gynecology, Social Insurance Saitama Chuo Hospital, Urawa, Jap...

    journal_title：Fertility and sterility

    pub_type： 杂志文章

    doi：10.1016/s0015-0282(99)00324-6

    authors： Inagaki N,Sato K,Toyoshima K,Ito K,Kitai H,Gabionza DT,Yoshimura Y

    更新日期：1999-10-01 00:00:00

  • Extensive tissue damage of bovine ovaries after bipolar ovarian drilling compared to monopolar electrocoagulation or carbon dioxide laser.

    abstract：OBJECTIVE:To evaluate the size of ovarian damage caused by ovarian drilling in polycystic ovary syndrome, the amount of inflicted damage was assessed for the most frequently used ovarian drilling techniques. DESIGN:Experimental prospective design. SETTING:University clinic. PATIENT(S):Six fresh bovine ovaries per te...

    journal_title：Fertility and sterility

    pub_type： 杂志文章

    doi：10.1016/j.fertnstert.2008.10.046

    authors： Hendriks ML,van der Valk P,Lambalk CB,Broeckaert MA,Homburg R,Hompes PG

    更新日期：2010-02-01 00:00:00

  • Frequency of premature menopause in women who carry a BRCA1 or BRCA2 mutation.

    abstract：OBJECTIVE:To evaluate the impact of carrying a BRCA1 or BRCA2 mutation on the probability of experiencing premature natural menopause. DESIGN:Observational study. SETTING:Patients in an academic research environment. PATIENT(S):Women who carry a BRCA1 or BRCA2 mutation (case subjects) and women who do not carry a mu...

    journal_title：Fertility and sterility

    pub_type： 杂志文章,多中心研究

    doi：10.1016/j.fertnstert.2013.01.109

    authors： Finch A,Valentini A,Greenblatt E,Lynch HT,Ghadirian P,Armel S,Neuhausen SL,Kim-Sing C,Tung N,Karlan B,Foulkes WD,Sun P,Narod S,Hereditary Breast Cancer Study Group.

    更新日期：2013-05-01 00:00:00

  • Preconceptional antithyroid peroxidase antibodies, but not thyroid-stimulating hormone, are associated with decreased live birth rates in infertile women.

    abstract：OBJECTIVE:To study whether preconceptual thyroid-stimulating hormone (TSH) and antithyroid peroxidase (TPO) antibodies are associated with poor reproductive outcomes in infertile women. DESIGN:Secondary analysis of data from two multicenter, randomized, controlled trials conducted by the Reproductive Medicine Network ...

    journal_title：Fertility and sterility

    pub_type： 杂志文章

    doi：10.1016/j.fertnstert.2017.08.026

    authors： Seungdamrong A,Steiner AZ,Gracia CR,Legro RS,Diamond MP,Coutifaris C,Schlaff WD,Casson P,Christman GM,Robinson RD,Huang H,Alvero R,Hansen KR,Jin S,Eisenberg E,Zhang H,Santoro N,Eunice Kennedy Shriver National Institute

    更新日期：2017-10-25 00:00:00

  • The effect of baseline complex ovarian cysts on in vitro fertilization outcome.

    abstract：STUDY OBJECTIVE:To determine the effect of baseline complex ovarian cysts on controlled ovarian hyperstimulation and in vitro fertilization (IVF) outcome. DESIGN:Retrospective analysis with stratification by stimulation regimen and the presence or absence of surgically documented endometriosis. PATIENTS:Two hundred s...

    journal_title：Fertility and sterility

    pub_type： 杂志文章

    doi：

    authors： Stewart EA,Jackson KV,Friedman AJ,Rein MS,Fox JH,Hornstein MD

    更新日期：1992-06-01 00:00:00

  • Whole genome deoxyribonucleic acid microarray analysis of gene expression in ectopic versus eutopic endometrium.

    abstract：OBJECTIVE:To use DNA microarrays to identify differentially expressed genes in eutopic endometrium compared with ectopic endometrium. DESIGN:Prospective, cross-sectional, observational study. SETTING:University Medical Center and Research Laboratory. PATIENT(S):Eleven women with endometriosis. INTERVENTION(S):None....

    journal_title：Fertility and sterility

    pub_type： 杂志文章

    doi：10.1016/j.fertnstert.2007.01.056

    authors： Eyster KM,Klinkova O,Kennedy V,Hansen KA

    更新日期：2007-12-01 00:00:00

  • Treatment of functional amenorrhea-galactorrhea with 2-bromoergocryptine.

    abstract：:The present study was undertaken to investigate not only the effectiveness of bromoergocryptine therapy in 13 women with amenorrhea-galactorrhea and hyperprolactinemia without evidence of organic pathology, but also to assess the value of pretreatment evaluation in predicting the response to therapy. Sella turcica tom...

    journal_title：Fertility and sterility

    pub_type： 杂志文章

    doi：10.1016/s0015-0282(16)42490-8

    authors： Wiebe RH,Hammond CB,Handwerger S

    更新日期：1977-04-01 00:00:00

  • HOXA-10 and E-cadherin expression in the endometrium of women with recurrent implantation failure and recurrent miscarriage.

    abstract：OBJECTIVE:To compare the expression of HOXA-10 and E-cadherin in the endometrium of women with recurrent implantation failure (RIF), women with recurrent miscarriage (RM), and women with proven fertility (normal control; NC). DESIGN:Observational cohort study. SETTING:University assisted reproductive unit. PATIENT(S...

    journal_title：Fertility and sterility

    pub_type： 杂志文章

    doi：10.1016/j.fertnstert.2016.09.016

    authors： Yang Y,Chen X,Saravelos SH,Liu Y,Huang J,Zhang J,Li TC

    更新日期：2017-01-01 00:00:00

  • The effect of the biochemical marker soluble human leukocyte antigen G on pregnancy outcome in assisted reproductive technology--a multicenter study.

    abstract：OBJECTIVE:To determine whether the presence of soluble human leukocyte antigen G (sHLA-G) affects implantation and pregnancy outcomes in vitro. DESIGN:A multicenter retrospective study. SETTING:Six certified in vitro fertilization (IVF) units. PATIENT(S):Embryos obtained from 2,040 patients from six different IVF cl...

    journal_title：Fertility and sterility

    pub_type： 杂志文章,多中心研究

    doi：10.1016/j.fertnstert.2013.07.1977

    authors： Kotze D,Kruger TF,Lombard C,Padayachee T,Keskintepe L,Sher G

    更新日期：2013-11-01 00:00:00

  • Amenorrhea in Olympic marathon runners.

    abstract：:Women who exercise heavily may develop secondary amenorrhea. Since the mechanism of so-called "runner's amenorrhea" has not been conclusively established, the authors examined the occurrence of amenorrhea in one of the most intensively exercising groups of female runners in the United States (average, 70 miles/week): ...

    journal_title：Fertility and sterility

    pub_type： 杂志文章

    doi：

    authors： Glass AR,Deuster PA,Kyle SB,Yahiro JA,Vigersky RA,Schoomaker EB

    更新日期：1987-11-01 00:00:00