Micromanipulation of sperm by a laser generated optical trap.


:The force generated by the radiation pressure of a low power laser beam induces an optical trap which may be used to manipulate sperm. We studied the effect of the optical trap on sperm motility. A Nd:YAG laser beam was coupled to a conventional microscope and focused into the viewing plane by the objective lens. Sperm were caught in the trap and manipulated by a joy stick controlled motorized stage. After different exposure periods, the velocity and patterns were analysed by a computerized image processor. There were minor changes in sperm velocity when exposed to the trap for 30 seconds or less. A gradual decrease in the mean linear velocity was observed after 45 seconds of exposure. This optical micromanipulator may also be useful for studying the force generated by a single spermatozoa and evaluating the influence of drugs on motility.


Fertil Steril


Fertility and sterility


Tadir Y,Wright WH,Vafa O,Ord T,Asch RH,Berns MW


Has Abstract


1989-11-01 00:00:00














  • The efficiency of transvaginal ultrasonography in the diagnosis of endometrioma.

    abstract:OBJECTIVE:To assess the efficiency of transvaginal ultrasonography in the screening of endometriomas and in differentiating endometriomas from other ovarian masses. DESIGN:Prospective study with surgical and pathological confirmation of the sonographic diagnosis. SETTING:Department of Obstetrics and Gynecology at the...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Mais V,Guerriero S,Ajossa S,Angiolucci M,Paoletti AM,Melis GB

    更新日期:1993-11-01 00:00:00

  • Hyaluronic acid binding and acrosin activity are decreased in sperm from men with spinal cord injury.

    abstract::In this study, acrosin activity and hyaluronic acid binding were compared in sperm from men with spinal cord injury versus sperm from healthy controls. The results showed statistically significantly lower acrosin activity and statistically significantly lower hyaluronic acid binding in spinal cord injured patients com...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Iremashvili V,Brackett NL,Ibrahim E,Aballa TC,Bruck D,Lynne CM

    更新日期:2010-10-01 00:00:00

  • Freeze-all policy: fresh vs. frozen-thawed embryo transfer.

    abstract:OBJECTIVE:To compare in vitro fertilization (IVF) outcomes between fresh embryo transfer (ET) and frozen-thawed ET (the "freeze-all" policy), with fresh ET performed only in cases without progesterone (P) elevation. DESIGN:Prospective, observational, cohort study. SETTING:Private IVF center. PATIENT(S):A total of 53...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Roque M,Valle M,Guimarães F,Sampaio M,Geber S

    更新日期:2015-05-01 00:00:00

  • Use of transperineal fine needle aspiration of seminal vesicles to retrieve sperm in a man with obstructive azoospermia.

    abstract:OBJECTIVE:To report the successful and feasible use of transperineal fine needle aspiration of seminal vesicles (FNASV) for sperm retrieval in obstructive azoospermia. DESIGN:Case report. SETTING:Outpatient care in institutional clinic. PATIENT(S):A 31-year-old man with obstructive azoospermia due to a middle prosta...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Cerruto MA,Novella G,Antoniolli SZ,Zattoni F

    更新日期:2006-12-01 00:00:00

  • Infertility among women working in horticulture. A follow-up study in the Danish Occupational Hospitalization Register.

    abstract::The possible association between employment in horticulture with potential exposure to pesticides and female infertility was examined by identification of women with hospital contact due to infertility and working in horticulture through the Danish Occupational Hospitalization Register. This follow-up study gave a sta...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Hougaard KS,Hannerz H,Feveile H,Bonde JP,Burr H

    更新日期:2009-04-01 00:00:00

  • Effect of hysteroscopy on the peritoneal dissemination of endometrial cancer cells: a meta-analysis.

    abstract:OBJECTIVE:To determine whether preoperative hysteroscopic examination increases the risk for peritoneal dissemination of endometrial cancer cells and the effect of hysteroscopy on disease prognosis. DESIGN:Meta-analysis. SETTING:Literature search conducted via domestic and international databases for studies on preop...

    journal_title:Fertility and sterility

    pub_type: 杂志文章,meta分析


    authors: Chang YN,Zhang Y,Wang YJ,Wang LP,Duan H

    更新日期:2011-10-01 00:00:00

  • Asymmetric dimethylarginine levels and carotid intima-media thickness in obese patients with polycystic ovary syndrome and their relationship to metabolic parameters.

    abstract:OBJECTIVE:To evaluate the plasma asymmetric dimethyl arginine (ADMA) levels and carotid intima-media thickness (IMT) in patients with polycystic ovary syndrome (PCOS). DESIGN:Prospective study. SETTING:University medical center. PATIENT(S):Thirty-five patients with PCOS and 31 healthy controls. INTERVENTION(S):Peri...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Pamuk BO,Torun AN,Kulaksizoglu M,Ertugrul D,Ciftci O,Kulaksizoglu S,Yildirim E,Demirag NG

    更新日期:2010-03-01 00:00:00

  • Significance of sperm characteristics in the evaluation of male infertility.

    abstract:OBJECTIVE:To compare sperm characteristics among: patients undergoing infertility evaluation, patients with male factor infertility (MFI), healthy sperm donors, and men with proven fertility; to examine the overlap of sperm characteristics in all four of these groups; and to identify good discriminators of fertility ve...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Nallella KP,Sharma RK,Aziz N,Agarwal A

    更新日期:2006-03-01 00:00:00

  • Subcutaneously administered Repronex in female patients undergoing in vitro fertilization is as effective and well tolerated as intramuscular menotropin treatment. Repronex SC, IVF Study Group.

    abstract:OBJECTIVE:To determine the efficacy and safety of Repronex SC as compared with Repronex IM and Pergonal IM in patients undergoing IVF. DESIGN:Open-label, randomized, parallel-group, multicenter study. SETTING:Fifteen academic and private fertility clinics with IVF experience. PATIENT(S):Premenopausal women with regu...

    journal_title:Fertility and sterility

    pub_type: 临床试验,杂志文章,多中心研究,随机对照试验


    authors: Gocial B,Keye WR,Fein SH,Nardi RV

    更新日期:2000-07-01 00:00:00

  • Interruption of pregnancy by prostaglandin 15-methyl F2alpha.

    abstract::The current study was formulated to investigate the abortifacient activity of prostaglandin 15-methyl F2alpha (15-methyl PGF2alpha) administered intramuscularly to 80 healthy women with gestations between 8 and 22 weeks. Goals were the establishment of an effective dosage schedule and assessment of the incidence and s...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Bolognese RJ,Corson SL

    更新日期:1975-07-01 00:00:00

  • Genetic screening of prospective oocyte donors.

    abstract:OBJECTIVE:To report our experience with genetic screening of oocyte donor candidates and to determine the frequency with which significant genetic issues are identified. DESIGN:Prospective genetic screening of oocyte donor candidates. SETTING:University hospital oocyte donation program. PATIENT(S):Women presenting c...

    journal_title:Fertility and sterility

    pub_type: 临床试验,杂志文章


    authors: Wallerstein R,Jansen V,Grifo JA,Berkeley AS,Noyes N,Licker J,Licciardi F

    更新日期:1998-07-01 00:00:00

  • Separation efficiency of a microfluidic sperm sorter to minimize sperm DNA damage.

    abstract:OBJECTIVE:To evaluate whether microfluidic sperm sorters (MFSSs) allow effective recovery of sorted motile sperm without DNA damage compared with the centrifugation and swim-up procedure. DESIGN:Experimental laboratory study. All participants completed questionnaires regarding previous and/or current diseases, surgery...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Shirota K,Yotsumoto F,Itoh H,Obama H,Hidaka N,Nakajima K,Miyamoto S

    更新日期:2016-02-01 00:00:00

  • HOXA-10 and E-cadherin expression in the endometrium of women with recurrent implantation failure and recurrent miscarriage.

    abstract:OBJECTIVE:To compare the expression of HOXA-10 and E-cadherin in the endometrium of women with recurrent implantation failure (RIF), women with recurrent miscarriage (RM), and women with proven fertility (normal control; NC). DESIGN:Observational cohort study. SETTING:University assisted reproductive unit. PATIENT(S...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Yang Y,Chen X,Saravelos SH,Liu Y,Huang J,Zhang J,Li TC

    更新日期:2017-01-01 00:00:00

  • Oxytocin-induced cell growth proliferation in human myometrial cells and leiomyomas.

    abstract:OBJECTIVE:To assess the expression of the oxytocin receptor (OTR) and the role of oxytocin (OT) in the proliferation of myometrial and leiomyoma cells. DESIGN:Prospective laboratory study. SETTING:Research laboratory at the Italian National Research Council. PATIENT(S):Twenty-two women who underwent therapeutic myom...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Busnelli M,Rimoldi V,Viganò P,Persani L,Di Blasio AM,Chini B

    更新日期:2010-10-01 00:00:00

  • BRCA carriers have similar reproductive potential at baseline to noncarriers: comparisons in cancer and cancer-free cohorts undergoing fertility preservation.

    abstract:OBJECTIVE:To investigate whether BRCA carriers with and without malignancy have decreased ovarian reserve at baseline compared with BRCA noncarriers. DESIGN:Retrospective cohort study. SETTING:Academic medical center. PATIENT(S):Seven-hundred and ninety-five oocyte cryopreservation patients, comprising BRCA carriers...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Gunnala V,Fields J,Irani M,D'Angelo D,Xu K,Schattman G,Rosenwaks Z

    更新日期:2019-02-01 00:00:00

  • Combined use of progesterone and human chorionic gonadotropin determinations for differential diagnosis of very early pregnancy.

    abstract::Progesterone (P) level and daily change in human chorionic gonadotropin (hCG) were determined in the serum of 307 patients with suspected ectopic pregnancy (EP). Of the viable intrauterine pregnancies (IUP), 99% had P values above 30 nmol/L, whereas 75% of the EP and 81% of the spontaneous abortions had P values less ...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Hahlin M,Sjöblom P,Lindblom B

    更新日期:1991-03-01 00:00:00

  • Exaggerated effects of progestogen on uterine artery pulsatility index in Turner's syndrome patients receiving hormone replacement therapy.

    abstract:OBJECTIVE:To investigate the effect of estrogen and progestogen on the resistance to blood flow in the uterine arteries of Turner's syndrome patients. DESIGN:Prospective clinical study. SETTING:A tertiary infertility clinic. PATIENTS:Five Turner's syndrome patients, six patients who had surgical castration, and five...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Biljan MM,Taylor CT,Matijevic R,Jones SV,Garden AS,Fraser WD,Diver MJ,Kingsland CR

    更新日期:1995-12-01 00:00:00

  • Repeated in vitro fertilization cycle attempts in patients undergoing controlled ovarian hyperstimulation with use of gonadotropin-releasing hormone antagonists.

    abstract::To examine pregnancy rate in patients undergoing controlled ovarian hyperstimulation (COH) with use of multidose GnRH antagonists in multiple repeated IVF cycle attempts, we conducted a survey in 785 consecutive IVF cycles in our unit. Although pregnancy rate significantly decreased beyond the second IVF cycle attempt...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Rabinson J,Ashkenazi J,Homburg R,Meltcer S,Anteby EY,Orvieto R

    更新日期:2009-04-01 00:00:00

  • In vitro maturation (IVM) of murine and human germinal vesicle (GV)-stage oocytes by coculture with immortalized human fallopian tube epithelial cells.

    abstract:OBJECTIVE:To improve the maturation rate of murine and human germinal vesicle (GV) oocytes using human tubal epithelial cells (hTECs). DESIGN:Murine and human GV oocytes were randomized to human tubal fluid (HTF) media alone or cocultured with mouse embryonic fibroblasts (MEFs) or primary hTECs or immortalized hTECS (...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Lee HJ,Quaas AM,Wright DL,Toth TL,Teixeira JM

    更新日期:2011-03-15 00:00:00

  • Partial androgen insensitivity--are all tissues equal?

    abstract::Nonaromatizable androgens, administered in high doses to an adult patient with partial androgen insensitivity, failed to result in a change in phallic size despite a clear decline in SHBG and gonadotropin levels. These findings raise the question of differential tissue sensitivity to androgens. Because ancillary labor...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Keely EJ,Belsham DD,Wrogemann K,Faiman C

    更新日期:1993-08-01 00:00:00

  • Endometrial receptivity in an in vitro fertilization program as assessed by spiral artery blood flow, endometrial thickness, endometrial volume, and uterine artery blood flow.

    abstract:OBJECTIVE:To investigate the role of sonographic parameters in assessing endometrial receptivity in an in vitro fertilization (IVF) program. DESIGN:Prospective clinical study. SETTING:University setting. PATIENT(S):One hundred thirty-five patients in our IVF program, selected prospectively on the day of oocyte retri...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Schild RL,Knobloch C,Dorn C,Fimmers R,van der Ven H,Hansmann M

    更新日期:2001-02-01 00:00:00

  • Transferrin and somatomedin C receptors in the human ovarian follicles.

    abstract::Transferrin and somatomedin C receptors (TFr and SMCr) were studied in human ovaries by immunostaining using monoclonal antisera. The oocyte of evoluting follicles was intensely positive for both types of receptors, but this positivity decreases in large follicles. An evident positivity for both TFr and SMCr was shown...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Balboni GC,Vannelli GB,Barni T,Orlando C,Serio M

    更新日期:1987-11-01 00:00:00

  • Nulliparity, fertility treatments and twins: a time for rethinking.

    abstract:OBJECTIVE:To evaluate the risk that nulliparity poses to the outcome of twin pregnancies, an issue that gained importance due to the rise of twin gestations following assisted reproduction interventions. DESIGN:A prospective cohort study. SETTING AND PATIENT(S):Between January 1, 2004, and January 7, 2008, we prospec...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Berkovitz A,Hershko-Klement A,Fejgin M

    更新日期:2010-04-01 00:00:00

  • Endometrial microbiota-new player in town.

    abstract::Detection of bacteria with molecular techniques has enabled the study of low biomass microbiomes in tissues and organs previously considered sterile, such as the endometrium. Subsequently, an abnormal endometrial microbiota has been associated with implantation failure, pregnancy loss, and other gynecological and obst...

    journal_title:Fertility and sterility

    pub_type: 杂志文章,评审


    authors: Moreno I,Franasiak JM

    更新日期:2017-07-01 00:00:00

  • Improved controlled ovarian hyperstimulation in poor responder in vitro fertilization patients with a microdose follicle-stimulating hormone flare, growth hormone protocol.

    abstract:OBJECTIVE:To assess the efficacy of a novel protocol-microdose GnRH agonist (GnRH-a), FSH, and GH, for the stimulation of IVF patients who were canceled previously on a standard luteal GnRH-a, FSH, GH protocol. DESIGN:Prospective evaluation using the patient's previous IVF stimulation attempt as historic controls. SE...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Schoolcraft W,Schlenker T,Gee M,Stevens J,Wagley L

    更新日期:1997-01-01 00:00:00

  • Prenatal hyperandrogenism and lipid profile during different age stages: an experimental study.

    abstract:OBJECTIVE:The present study investigates the effect of prenatal hyperandrogenization on lipid metabolism and oxidant/antioxidant balance. DESIGN:Experimental study. SETTING:Research institute. ANIMAL(S):Pregnant Sprague Dawley rats were subcutaneously injected with 2 mg free T between days 16 and 19 of pregnancy, an...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Heber MF,Ferreira SR,Vélez LM,Motta AB

    更新日期:2013-02-01 00:00:00

  • Preimplantation genetic diagnosis increases the implantation rate in human in vitro fertilization by avoiding the transfer of chromosomally abnormal embryos.

    abstract:OBJECTIVE:To verify the percentage of chromosomally abnormal preimplantation embryos in patients with a poor prognosis and possibly to increase the chance of implantation by selecting chromosomally normal embryos. DESIGN:A prospective, randomized, controlled study. SETTING:In vitro fertilization program at the Reprod...

    journal_title:Fertility and sterility

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Gianaroli L,Magli MC,Ferraretti AP,Fiorentino A,Garrisi J,Munné S

    更新日期:1997-12-01 00:00:00

  • Increased odds of live birth in fresh in vitro fertilization cycles with shorter ovarian stimulation.

    abstract:OBJECTIVE:To investigate the impact of prolonged ovarian stimulation on pregnancy outcomes in IVF cycles with fresh day 3 ET. DESIGN:Retrospective cohort study. SETTING:University-affiliated center. PATIENT(S):All patients initiating their first IVF cycle with fresh day 3 ET. Prolonged ovarian stimulation was define...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Pereira N,Friedman C,Hutchinson AP,Lekovich JP,Elias RT,Rosenwaks Z

    更新日期:2017-01-01 00:00:00

  • Gamete intrafallopian tube transfer (GIFT): making laparoscopy more than "diagnostic".

    abstract::Diagnostic laparoscopy is commonly performed on patients as part of a complete infertility investigation. Recently published protocols have investigated the efficacy of using empiric ovulation induction, intrauterine insemination, or both before beginning in vitro fertilization. Because many patients enrolled in these...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Barad DH,Bartfai G,Barg P,Cohen BL,Feinman M

    更新日期:1988-12-01 00:00:00

  • Biological evaluation of a novel copper-containing composite for contraception.

    abstract:OBJECTIVE:To investigate the biocompatibility of a novel copper-containing composite to provide preclinical data for clinical application of intrauterine device (IUD) or intra-vas device (IVD). DESIGN:Prospective experimental study. SETTING:Good laboratory practices laboratories. ANIMALS:Twenty healthy adult mice (S...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Sun L,Huang XB,Suo JP,Fan BL,Chen ZL,Yang WX,Li J

    更新日期:2011-03-15 00:00:00