Abstract：
OBJECTIVE:Immunobead testing (IBT), the current standard for antisperm antibody detection, is time consuming and somewhat subjective. To overcome these limitations and maintain accuracy, we studied an immunofluorescent assay using flow cytometry. DESIGN:A validation study comparing flow cytometry to IBT in the detection of serum antisperm antibodies. SETTING:Flow cytometry laboratory. PATIENTS:Sera from 37 men after vasectomy (test) and sera from 35 fertile men (control). MAIN OUTCOME MEASURE:Test serum with and without immunoglobulin (Ig)G, IgA, and IgM antisperm antibodies as defined by IBT were analyzed by flow cytometry. Sensitivity and specificity of flow cytometry was calculated by defining the IBT as the true result. RESULTS:Flow cytometry identified 22 of 22 sera that were IgG positive (100% sensitivity), 12 of 14 sera that were IgA positive (86% sensitivity), and 4 of 4 sera that were IgM positive (100% sensitivity). Overall, 22 of 37 men were positive for antisperm antibodies. The flow cytometry correctly identified 71 of 71 negative sera (100% specificity). Fluorescence intensity values from the 37 study patients significantly correlated with immunobead binding to the head region and to the entire (more than one) region. CONCLUSIONS:Detection of IgG, IgA, and IgM antisperm antibodies by flow cytometry is highly sensitive and specific. In addition, flow cytometry is able to assess thousands of sperm rapidly and accurately, reducing sampling error and technical time.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Ke RW,Dockter ME,Majumdar G,Buster JE,Carson SAsubject
Has Abstractpub_date
1995-04-01 00:00:00pages
902-6issue
4eissn
0015-0282issn
1556-5653pii
S0015-0282(16)57501-3journal_volume
63pub_type
杂志文章abstract：OBJECTIVE:To determine the contribution of several variables to fluid loss during transcervical resection of submucous myomas. DESIGN:An observational study using multiple linear regression analyses. SETTING:A university-affiliated training hospital and a university department of clinical epidemiology and biostatisti...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(97)00335-x
更新日期：1997-11-01 00:00:00
abstract：:When dispersed spermatogenic cells obtained by enzymatic digestion from prepuberal mice, adult male mice, nonazoospermic men and normospermic men were observed live using Normarski optics, it was found that, respectively, 47.4%, 1.4%, 5.1%, and 2.4% of them protruded active pseudopodia. These cells were 8 to 10 mum in...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2007.06.055
更新日期：2008-08-01 00:00:00
abstract：:A retrospective study of 254 infertile men with serum sperm agglutination titers ranging from 4 to 1024 determined by the Kibrick agglutination test between 1954 and 1968 and reexamined in the 1970s is presented. 36 of the subjects had become fertile in the years following the testing, 30 of whom were normospermic. ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)40388-2
更新日期：1974-05-01 00:00:00
abstract：OBJECTIVE:To determine the prevalence of microdeletions of the long arm of chromosome Y within the AZFa, AZFb, and AZFc subregions in patients with idiopathic azoospermia, and then correlate the microdeletions with clinical phenotypes to determine the most important subregion for screening. DESIGN:Controlled clinical ...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章
doi：10.1016/s0015-0282(01)01955-0
更新日期：2001-09-01 00:00:00
abstract：OBJECTIVE:To examine the relationship between MMPI-2 scores and oocyte donation outcome. DESIGN:Descriptive chart review. SETTING:Two oocyte donation programs. SUBJECT(S):Five hundred anonymous oocyte donor applicants. INTERVENTION(S):None. MAIN OUTCOME MEASURE(S):Demographics, MMPI-2 scores and donation outcome. ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2009.08.064
更新日期：2010-10-01 00:00:00
abstract：OBJECTIVE:To describe the detection of Hodgkin lymphoma within ovarian tissue taken at the time of harvest for cryopreservation. DESIGN:Case report. SETTING:University-affiliated women's hospital. PATIENT(S):A 19-year-old woman diagnosed with Hodgkin lymphoma. INTERVENTION(S):Laparoscopic removal of ovarian tissue ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2010.07.1068
更新日期：2011-02-01 00:00:00
abstract：OBJECTIVE:To evaluate the use of sonohysterography for uterine screening before IVF. DESIGN:Prospective screening with sonohysterography and comparison with available hysterosalpingographic and hysteroscopic evaluations. SETTING:Private practice. PATIENT(S):Seventy-two women undergoing IVF-ET using their own or dono...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(98)00045-4
更新日期：1998-05-01 00:00:00
abstract：OBJECTIVE:To develop and apply efficient and reliable protocols for preimplantation genetic diagnosis (PGD) for Marfan syndrome. DESIGN:Two mutation-specific protocols were developed, and the markers D15S1028, D15S992, D15S196, D15S576, D15S123, and D15S143 were used to set up four multiplex polymerase chain reactions...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2005.12.059
更新日期：2006-08-01 00:00:00
abstract：OBJECTIVE:To determine whether nerve fibers can be detected in the endometrium and myometrium in women with painful uterine fibroids and adenomyosis. DESIGN:A retrospective immunohistochemical study. SETTING:An academic training hospital. PATIENT(S):Thirty-seven women with uterine fibroids and 29 women with adenomyo...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2009.03.026
更新日期：2010-07-01 00:00:00
abstract：OBJECTIVE:To report a case of laparoscopic treatment of a heterotopic primary abdominal pregnancy after IVF with preservation of the concurrent intrauterine pregnancy. DESIGN:Case report. SETTING:University-based IVF program. PATIENT(S):A woman with a heterotopic abdominal pregnancy after IVF-ET. INTERVENTION(S):Pi...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(98)00104-6
更新日期：1998-07-01 00:00:00
abstract：OBJECTIVE:To investigate the effect of vitrification and beta-mercaptoethanol (beta-ME) on reactive oxygen species (ROS) activity and in vitro development of oocytes vitrified before or after in vitro fertilization (IVF). DESIGN:Randomized prospective study. SETTING:University-based assisted reproductive technology l...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2010.01.043
更新日期：2010-05-15 00:00:00
abstract：OBJECTIVE:To describe how parents envision, plan, and enact disclosing to their children that they were conceived with donor gametes. DESIGN:In-depth ethnographic interviews. SETTING:Participants were recruited from 11 medical infertility practices and 1 sperm bank in Northern California. PATIENT(S):A total of 141 m...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2006.07.1514
更新日期：2007-03-01 00:00:00
abstract：:13 women with suspected hypothalamic anovulation were treated with l uteinizing hormone-releasing hormone (LH-RH) by a variety of regimens. 3 women received LH-RH by continuous intravenous infusion equal to 100 mcg LH-RH for 8 hours, followed by an acute injection of LH-RH 100 mcg. 10 days later the infusion was rep...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)39196-8
更新日期：1972-09-01 00:00:00
abstract：OBJECTIVE:To determine whether inhibition of epidermal growth factor (EGF) receptor tyrosine kinase with lapatinib affects oocyte maturation, expression of the cumulus expansion-associated genes such as tumor necrosis factor alpha-induced protein 6 (TNFAIP6) and prostaglandin-endoperoxide synthase 2 (PTGS2), and synthe...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2012.12.040
更新日期：2013-05-01 00:00:00
abstract：:Oocytes are complex cells comprising many cellular systems, each of which is essential for proper oocyte function. Methods to assess the diverse and critical cellular systems in oocytes derived from in vitro maturation are badly needed. ...
journal_title：Fertility and sterility
pub_type： 评论,杂志文章,评审
doi：10.1016/j.fertnstert.2005.12.009
更新日期：2006-04-01 00:00:00
abstract：:Elevation of serum luteinizing hormone levels was observed in five normal male volunteers while they were taking Centchroman. This effect resembles that of clomiphene and suggests a new biologic potential of Centchroman. ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)41785-1
更新日期：1976-04-01 00:00:00
abstract：OBJECTIVE:To evaluate whether intravaginal application of seminal plasma at the time of follicle aspiration in IVF or intracytoplasmic sperm injection treatment cycles has the potential to increase pregnancy rate. To calculate the number of patients needed to achieve significance in a multicenter trial. DESIGN:Double-...
journal_title：Fertility and sterility
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1016/j.fertnstert.2007.11.036
更新日期：2009-01-01 00:00:00
abstract：:Turner syndrome (TS), which affects approximately 1 in 2,500 live-born females, is characterized by loss or structural anomalies of an X chromosome. Clinical features vary among patients; multiple organ systems can be affected. Endocrinologists are involved in the management of short stature, delayed puberty, and infe...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
doi：10.1016/j.fertnstert.2012.07.1104
更新日期：2012-10-01 00:00:00
abstract：:Twelve women of normal weight (ages 17 to 36 years) with scanty menstrual bleeding were studied. They had no signs of virilization, gynecologic or endocrine pathology, or past history of hormonal treatment. Five women (group 1) experienced withdrawal bleeding after a 3-day course of chlormadinone acetate (2 mg/day) an...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)43213-9
更新日期：1978-04-01 00:00:00
abstract：OBJECTIVE:To investigate the impact of intracytoplasmic sperm injection (ICSI) and assisted hatching (AH) on assisted reproductive technology (ART) outcomes in initial cycles with diminished ovarian reserve (DOR) as the primary diagnosis. DESIGN:Retrospective cohort study of cycles from the Society for Assisted Reprod...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2014.06.043
更新日期：2014-10-01 00:00:00
abstract：OBJECTIVE:To test the hypothesis that plasma visfatin concentrations will be higher in women with polycystic ovary syndrome (PCOS) than in women without PCOS. DESIGN:Clinical study. SETTING:University hospital. PATIENT(S):A total of 52 women were evaluated. Twenty-six had PCOS, and the remaining 26 were healthy wome...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2006.11.120
更新日期：2007-08-01 00:00:00
abstract：OBJECTIVE:To evaluate whether stimulation of endometrium embryo transfer (SEET) can improve implantation rate and pregnancy rate (PR) for patients undergoing assisted reproductive technology (ART) for the first time by injecting embryo culture supernatant into the uterus before blastocyst transfer (BT). DESIGN:Randomi...
journal_title：Fertility and sterility
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.fertnstert.2008.08.076
更新日期：2009-10-01 00:00:00
abstract：OBJECTIVE:To describe a live birth obtained in Italy after autologous orthotopic transplantation of cryopreserved ovarian cortical tissue. DESIGN:Case report. SETTING:University department of gynecology and obstetrics, reproductive medicine and IVF unit. PATIENT(S):A 29-year-old patient affected by β-thalassemia (in...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2012.09.029
更新日期：2013-01-01 00:00:00
abstract：OBJECTIVE:To evaluate the predictive value of seminal inhibin B and anti-Müllerian hormone (AMH) on the outcome of testicular sperm extraction (TESE) in patients with nonobstructive azoospermia. DESIGN:Prospective study. SETTING:Reproductive biology department. PATIENT(S):Forty-seven normospermic, 28 oligozoospermic...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2007.03.032
更新日期：2008-02-01 00:00:00
abstract：:The key global issue surrounding gamete donation is not the well-rehearsed known vs. anonymous donation argument but rather one of providing patients with a flexible dual-track system (i.e., ability to know or not to know donor identity), complete with psychosocial support and education that enables them to make well-...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2005.07.1304
更新日期：2006-02-01 00:00:00
abstract：OBJECTIVE:To review the current understanding of the role the uterus plays in embryo implantation and to outline congenital anomalies and acquired diseases that impact normal uterine function. DESIGN:The publications related to the embryo implantation, Mullerian anomalies, uterine polyps, uterine synechiae, and myomas...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
doi：10.1016/j.fertnstert.2007.09.069
更新日期：2008-01-01 00:00:00
abstract：:We have previously observed that preimplantation embryo biopsy in the mouse causes a reduction in implantation rate in utero. After minor modifications to the technique, we now find that sampling a single blastomere from the 4-cell mouse embryo does not compromise continued development in vitro or in vivo. When transf...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)60564-2
更新日期：1989-03-01 00:00:00
abstract：OBJECTIVE:To describe an ovarian abscess presenting very late after oocyte retrieval for IVF with several unusual clinical features. DESIGN:Case report. SETTING:Academic medical center. PATIENT(S):A 35-year-old nulliparous woman underwent IVF with uncomplicated transvaginal oocyte retrieval (TVOR), resulting in a di...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2005.12.045
更新日期：2006-07-01 00:00:00
abstract：:Oocytes with three or more pronuclei and one or two polar bodies arising after intracytoplasmic sperm injection were analyzed cytogenetically, and the data were used to develop a general model explaining the possible origin of the abnormal pronuclear stages. The mechanisms, either separately or in variable combination...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2010.03.075
更新日期：2010-11-01 00:00:00
abstract：:Surgical alteration of rabbit oviducts was employed to investigate the mechanisms of ovum transport. Resection of a 5- to 10-mm segment of midampulla was followed by microsurgical end-to-end anastomosis. One to two months later, direct observations were made, in situ, of ampullary egg transport. Stained cumulus masses...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)44421-3
更新日期：1979-12-01 00:00:00
