Abstract:
OBJECTIVE:To develop and apply efficient and reliable protocols for preimplantation genetic diagnosis (PGD) for Marfan syndrome. DESIGN:Two mutation-specific protocols were developed, and the markers D15S1028, D15S992, D15S196, D15S576, D15S123, and D15S143 were used to set up four multiplex polymerase chain reactions (PCRs). SETTING:Research Center Reproduction and Genetics. PATIENT(S):Ten couples carrying mutations in the FBN1 gene. INTERVENTION(S):Six PGD protocols were developed for 10 couples, and 7 of them underwent a total of 16 clinical cycles. MAIN OUTCOME MEASURE(S):Amplification, allele drop-out (ADO), and contamination rates during the preclinical assays. DNA analyses of blastomeres from embryos biopsied during PGD cycles. RESULT(S):Six different protocols were set up, with the main objective being to to use one protocol for several couples. A total of 16 PGD cycles were performed, which resulted in the delivery of an unaffected boy and three ongoing pregnancies. CONCLUSION(S):The development of single-cell multiplex PCRs for linked markers and its use in PGD reduce the workload of the genetic diagnostic laboratory as well as the average waiting time for patients. This approach also allows for the simultaneous and accurate detection of recombination, contamination, and ADO, thereby increasing the reliability of the diagnosis.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Spits C,De Rycke M,Verpoest W,Lissens W,Van Steirteghem A,Liebaers I,Sermon Kdoi
10.1016/j.fertnstert.2005.12.059subject
Has Abstractpub_date
2006-08-01 00:00:00pages
310-20issue
2eissn
0015-0282issn
1556-5653pii
S0015-0282(06)00652-2journal_volume
86pub_type
杂志文章abstract:OBJECTIVE:To investigate whether levothyroxine (LT4) treatment has beneficial effects on IVF results and pregnancy outcome in infertile patients with subclinical hypothyroidism undergoing IVF/intracytoplasmic sperm injection (ICSI). DESIGN:Prospective, randomized trial. SETTING:University-affiliated infertility clini...
journal_title:Fertility and sterility
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.fertnstert.2010.12.004
更新日期:2011-04-01 00:00:00
abstract:OBJECTIVE:To investigate whether BRCA carriers with and without malignancy have decreased ovarian reserve at baseline compared with BRCA noncarriers. DESIGN:Retrospective cohort study. SETTING:Academic medical center. PATIENT(S):Seven-hundred and ninety-five oocyte cryopreservation patients, comprising BRCA carriers...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2018.10.014
更新日期:2019-02-01 00:00:00
abstract:OBJECTIVE:To establish a mouse model for endometriosis and to evaluate roles of vascular endothelial growth factor (VEGF) and matrix metalloproteinase-2 (MMP-2) in the formation of disease. DESIGN:Experimental laboratory study. SETTING:A women's hospital in China. PATIENT(S) AND ANIMAL(S):Ten women with endometriosi...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2005.12.083
更新日期:2006-10-01 00:00:00
abstract:OBJECTIVE:To examine the effect of a gonadotropin-releasing hormone agonist (GnRH-a), leuprolide acetate (LA), on human chorionic gonadotropin/luteinizing hormone (LH) receptors content and progesterone (P) and estradiol (E2) production in cultured granulosa or luteal cells. DESIGN:Prospective. SETTING:Private Fertil...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:
更新日期:1993-04-01 00:00:00
abstract:OBJECTIVE:To study the daily levels of salivary progesterone (P) during the menstrual cycles of adolescent girls 14 to 19 years of age and the occurrence of anovulations and defective luteal phases. DESIGN:The salivary P levels were analyzed by radioimmunoassay from three age groups of girls (n = 65) and compared with...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:
更新日期:1992-10-01 00:00:00
abstract:OBJECTIVE:To determine the size of the baboon corpus luteum (CL) and levels of plasma P, 17alpha-hydroxyprogesterone (17-OHP), and E2 in the ovarian vein draining it, the contralateral ovarian vein, and peripheral blood throughout the luteal phase of the menstrual cycle. DESIGN:Prospective study. SETTING:Academic dep...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2007.03.047
更新日期:2008-05-01 00:00:00
abstract::Both EST and Mayer-Rokitansky-Kuster-Hauser syndrome are uncommon disorders. This report illustrates a case of these two rare conditions occurring in the same patient. Patients with Mayer-Rokitansky-Kuster-Hauser syndrome, although not appearing to be at increased risk for germ cell tumors, unlike testicular feminizat...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)54562-2
更新日期:1991-09-01 00:00:00
abstract:OBJECTIVE:To determine the efficacy of treating women with severe menstrual migraine headaches with GnRH agonist (GnRH-a) therapy, alone and combined with continuous estrogen-progestin "add-back." DESIGN:Nonrandomized, prospective treatment study. SETTING:Outpatient clinic in a university medical center. PATIENT(S):...
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章
doi:10.1016/S0015-0282(97)81928-0
更新日期:1997-02-01 00:00:00
abstract::We studied endometrial luteal phase in specimens from 660 biopsies done in 300 patients from our infertility clinic. A minimum of two (240 women) or three (60 women) endometrial biopsy specimens from separate cycles were taken regardless of the previous histologic findings in all patients. Statistical analysis of resu...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)48990-9
更新日期:1985-11-01 00:00:00
abstract::Fifty-one female volunteers used a superactive stimulatory luteinizing hormone-releasing hormone (LH-RH) analog for suppression of ovulation for 3 to 12 months. The potent LH-RH agonist D-Ser(TBU)6-EA10-LH-RH was administered intranasally once daily in a dose of 400 or 600 micrograms. No pregnancies occurred during th...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:
更新日期:1982-08-01 00:00:00
abstract::Hypoxia downregulated the concentration of endostatin in the culture media of human endometrial stromal cells but did not affect the messenger (m)RNA expression of collagen XVIII. Both mRNA and protein expression of vascular endothelial growth factor were upregulated in a hypoxic condition. ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2004.03.032
更新日期:2004-09-01 00:00:00
abstract::Daily leukocyte alkaline phosphatase activity (LAP), basal body temperature (BBT), fern test (FT) and serum luteinizing hormone (LH) were determined during 15 normal menstrual cycles and 10 cycles treated with clomiphene citrate. A rise in LAP activity, as determined by LAP-relative score (LAP-RS), was observed durin...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)39623-6
更新日期:1973-04-01 00:00:00
abstract:OBJECTIVES:To evaluate prospectively the impact of subtle tubal disorders on GIFT pregnancy rates in women with pelvic endometriosis. DESIGN:A prospective comparison of pregnancy rates in different stages of endometriosis while controlling for the presence of tubal abnormalities. INTERVENTIONS:A total of 146 patients...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)57007-1
更新日期:1994-10-01 00:00:00
abstract::The outcome of a series of pre-embryo transfers to 31 women with ovarian failure is described. Twenty six fertile women functioned as nonanonymous donors, providing oocytes for in vitro fertilization after undergoing controlled ovarian hyperstimulation and transvaginal ultrasound directed oocyte aspiration. Recipients...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)54055-2
更新日期:1991-01-01 00:00:00
abstract:OBJECTIVE:To explore the long-term outcome of patients who began IVF treatment by considering not only treatment outcome in the center but also the parenthood project outcome after discontinuation of unsuccessful IVF. DESIGN:Retrospective cohort follow-up study. SETTING:Two French IVF centers. PATIENT(S):Seven hundr...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2008.05.067
更新日期:2009-07-01 00:00:00
abstract:OBJECTIVE:A challenge of in vitro maturation (IVM) treatment in some women is insufficient development of the endometrium prior to embryo transfer. DESIGN:Retrospective study. SETTING:McGill Reproductive Center, Montreal, Canada. PATIENT(S):Women with endometrial thickness <6 mm on days 6-10 ultrasound (US) scan of ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2008.07.1750
更新日期:2009-09-01 00:00:00
abstract:OBJECTIVE:To determine the level of standardization in performance of the semen analysis among clinical laboratories in the United States. DESIGN:A survey was mailed to laboratories requesting information about the laboratory and performance of the semen analysis. Responses were received from 536 laboratories. SETTIN...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(02)03296-x
更新日期:2002-09-01 00:00:00
abstract:OBJECTIVE:Report of a rare case of consecutive spontaneous cervical pregnancies. DESIGN:Case study. SETTING:Teaching hospital. PATIENT(S):One healthy nulliparous woman in the early years of her fourth decade. INTERVENTION(S):The first of two cervical pregnancies was treated with two doses of methotrexate and subseq...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2003.06.012
更新日期:2004-01-01 00:00:00
abstract:OBJECTIVE:To compare danazol and gestrinone treatment as preoperative endometrial preparation for operative hysteroscopy. DESIGN:Prospective, randomized clinical study. SETTING:University department of gynecological, obstetrical sciences and reproductive medicine. PATIENT(S):One hundred thirty-five patients with end...
journal_title:Fertility and sterility
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.fertnstert.2005.09.034
更新日期:2006-04-01 00:00:00
abstract::Encounters for infertility care are opportunities to assess and update immunization status. Women of reproductive age are often unaware of their need for immunization, their own immunization status, and the potentially serious consequences of preventable disease on pregnancy outcome. The purpose of this ASRM Practice ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2012.08.027
更新日期:2013-02-01 00:00:00
abstract:OBJECTIVE:To investigate the long-term effects of raloxifene on fasting plasma homocysteine levels in postmenopausal women compared with conjugated equine estrogen (CEE). DESIGN:Randomized, double-blind, placebo-controlled study. SETTING:Outpatient department of a university hospital. PATIENT(S):Fifty-two hysterecto...
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/s0015-0282(98)00381-1
更新日期:1998-12-01 00:00:00
abstract:OBJECTIVE:To evaluate whether adolescent obesity is associated with difficulties in becoming pregnant later in life. DESIGN:Cross-sectional analysis of baseline data from a longitudinal cohort. SETTING:Multiethnic, community-based observational study of U.S. women. PATIENT(S):Three thousand one hundred fifty-four mi...
journal_title:Fertility and sterility
pub_type: 杂志文章,多中心研究
doi:10.1016/j.fertnstert.2008.12.059
更新日期:2010-04-01 00:00:00
abstract:OBJECTIVE:To determine the relative efficacy of ultrasound-guided embryo transfer and embryo transfer by clinical touch alone. DESIGN:Systematic review and meta-analysis of randomized, controlled trials comparing ultrasound-guided embryo transfer with embryo transfer by clinical touch alone. SETTING:Infertility cente...
journal_title:Fertility and sterility
pub_type: 杂志文章,meta分析,评审
doi:10.1016/s0015-0282(03)01015-x
更新日期:2003-10-01 00:00:00
abstract:OBJECTIVE:To describe the reproductive health history and characteristics of women having bariatric surgery and to determine whether this differs by age of onset of obesity. DESIGN:Retrospective and cross-sectional analyses of self-reported survey data. SETTING:Six sites of the Longitudinal Assessment of Bariatric Su...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2009.08.028
更新日期:2010-09-01 00:00:00
abstract:OBJECTIVE:To determine whether the expression of leukemia inhibitory factor during the implantation window in endometrial tissue and uterine flushing fluid of patients with adenomyosis differs from that of healthy fertile women. DESIGN:Experimental study using endometrial tissue and uterine flushing fluid. SETTING:Pe...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2009.03.012
更新日期:2010-06-01 00:00:00
abstract:OBJECTIVE:To evaluate the mRNA levels of connexins in different sizes of luteinized follicles. DESIGN:Semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) to determine the connexin mRNA levels in the granulosa cells of 91 luteinized follicles. SETTING:Academic tertiary care medical center and res...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2003.05.015
更新日期:2003-12-01 00:00:00
abstract:OBJECTIVE:To determine the feasibility of asking women undergoing fertility treatment the maximum increased risk of ovarian cancer they would be willing to tolerate in order to take ovulation-induction drugs. DESIGN:A prospective pilot study of women attending fertility clinics over a 2-month period. SETTING:Two tert...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(97)81481-1
更新日期:1997-07-01 00:00:00
abstract::The current study was formulated to investigate the abortifacient activity of prostaglandin 15-methyl F2alpha (15-methyl PGF2alpha) administered intramuscularly to 80 healthy women with gestations between 8 and 22 weeks. Goals were the establishment of an effective dosage schedule and assessment of the incidence and s...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:
更新日期:1975-07-01 00:00:00
abstract:OBJECTIVE:To evaluate the outcome of repeated electroejaculation for obtaining semen from adolescents and young men before initiation of anticancer therapies. DESIGN:Retrospective clinical study. SETTING:Bikur Cholim Hospital, Jerusalem, Israel. PATIENT(S):Six young male patients (average age, 18+/-3 years) with dia...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(01)01680-6
更新日期:2001-04-01 00:00:00
abstract::The aim of the present study was to compare the levels of leukemia inhibitory factor (LIF) in the tubal ampullary region in normal and ectopic pregnancies and assess possible causative role of LIF in ectopic pregnancy. Although there was a tendency of increase in LIF concentrations in the ectopic pregnancy group, we w...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2004.09.014
更新日期:2005-02-01 00:00:00