Successful single-cell biopsy and cryopreservation of preimplantation mouse embryos.

Abstract:

:We have previously observed that preimplantation embryo biopsy in the mouse causes a reduction in implantation rate in utero. After minor modifications to the technique, we now find that sampling a single blastomere from the 4-cell mouse embryo does not compromise continued development in vitro or in vivo. When transferred to pseudopregnant foster mice, 60.3% and 64.3% of biopsied and control embryos, respectively, implanted into the uterine wall, and 52.6% and 52.4% of biopsied and control embryos, respectively, developed into fetuses. In a separate series of experiments, we have demonstrated that biopsied mouse embryos can be successfully cryopreserved by ultrarapid freezing even though they have a punctured zona pellucida. Biopsied (frozen-thawed), control (frozen-thawed), and nonfrozen embryos had an implantation rate of 81.1%, 74.3%, and 74.1%, respectively, and a fetal formation rate of 62.2%, 62.9%, and 66.7%, respectively.

journal_name

Fertil Steril

journal_title

Fertility and sterility

authors

Wilton LJ,Shaw JM,Trounson AO

doi

10.1016/s0015-0282(16)60564-2

subject

Has Abstract

pub_date

1989-03-01 00:00:00

pages

513-7

issue

3

eissn

0015-0282

issn

1556-5653

pii

S0015-0282(16)60564-2

journal_volume

51

pub_type

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