Abstract：
OBJECTIVE:To analyze the protein production into the surrounding medium (secretome) of both human and mouse embryos and correlate these findings with ongoing blastocyst development. Along with improvements in culture systems, there is renewed focus on the development of noninvasive viability assays in human IVF. Because the majority of biologic functions are carried out by proteins, it is important to study the dynamics of the proteome during embryonic development and its response to both internal and external stimuli. DESIGN:Experimental study. SETTING:Research laboratory. PATIENT(S):Couples undergoing infertility treatment donated with consent spent culture media for research. INTERVENTION(S):Analysis by time-of-flight mass spectrometry. MAIN OUTCOME MEASURE(S):Protein profiles of spent culture media. RESULT(S):Distinctive and significantly different secretome profiles were observed at each embryonic developmental stage (P<0.05). Correlation of day 5 secretome data with ongoing blastocyst development revealed an 8.5-kDa protein biomarker that was significantly up-regulated (P<0.05). The best candidate for this protein biomarker was ubiquitin, which has been implicated in the implantation process of mammalian species. CONCLUSION(S):This approach to analyze the secretome should not only further our understanding of embryo physiology but provide the basis for the development of noninvasive assays of embryo viability in human IVF.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Katz-Jaffe MG,Schoolcraft WB,Gardner DKdoi
10.1016/j.fertnstert.2006.05.022subject
Has Abstractpub_date
2006-09-01 00:00:00pages
678-85issue
3eissn
0015-0282issn
1556-5653pii
S0015-0282(06)01495-6journal_volume
86pub_type
杂志文章abstract：OBJECTIVE:To perform a systematic review and meta-analysis of studies that compared the prevalence of anxiety symptoms in women with polycystic ovary syndrome (PCOS) and control women. DESIGN:Meta-analysis and systematic review. SETTING:University practice. PATIENT(S):Cross-sectional studies comparing PCOS subjects ...
journal_title：Fertility and sterility
pub_type： 杂志文章,meta分析,评审
doi：10.1016/j.fertnstert.2011.10.022
更新日期：2012-01-01 00:00:00
abstract：OBJECTIVE:To evaluate the effect of reproductive history on the outcome of different procedures in assisted reproductive technologies (ART) comparing IVF, ICSI, and cryopreserved embryo transfer (CPE). DESIGN:Prospective registration of ART cycles and their outcomes. SETTING:One hundred three reproductive programs in...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(03)00760-x
更新日期：2003-09-01 00:00:00
abstract：:Patient requests for transfer of embryos with genetic anomalies linked to serious health-affecting disorders detected in preimplantation testing are rare but do exist. This Opinion sets out the possible rationales for a provider's decision to assist or decline to assist in such transfers. The Committee concludes in mo...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2017.02.121
更新日期：2017-05-01 00:00:00
abstract：:In this study, both IVF-ET cycle and pregnancy outcome were compared with increases seen in P concentration during the 24-hour period from 12 hours before to 12 hours after hCG administration. A significantly higher PR (P < 0.001) was seen in women who had at least a threefold increase in P during this time period. Fu...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1994-11-01 00:00:00
abstract：OBJECTIVE:To evaluate the effect of sperm treatment with exogenous platelet-activating factor (PAF) on IUI clinical pregnancy rate. DESIGN:Prospective randomized study. SETTING:Assisted Reproduction Unit, 2nd Department of Obstetrics and Gynecology, University of Athens, Aretaieion Hospital, Athens, Greece. SUBJECT(...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/j.fertnstert.2004.07.980
更新日期：2005-03-01 00:00:00
abstract：:Oocytes are complex cells comprising many cellular systems, each of which is essential for proper oocyte function. Methods to assess the diverse and critical cellular systems in oocytes derived from in vitro maturation are badly needed. ...
journal_title：Fertility and sterility
pub_type： 评论,杂志文章,评审
doi：10.1016/j.fertnstert.2005.12.009
更新日期：2006-04-01 00:00:00
abstract：OBJECTIVE:To study the effect of mifepristone on heparin-binding epidermal growth factor (HB-EGF) and its receptors HER1 and HER4 in the fallopian tube and in the endometrium. DESIGN:Prospective clinical study. SETTING:Hospital-based unit for obstetrics and gynecology and research laboratories. PATIENT(S):Healthy wo...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2005.08.011
更新日期：2006-01-01 00:00:00
abstract：OBJECTIVE:To compare the effectiveness and safety of the Filshie Clip and Tubal Ring systems when applied via minilaparotomy and laparoscopy. DESIGN:Prospective, multicenter randomized controlled clinical trial, with postoperative evaluation by a physician who was masked to the operative technique. SETTING:Healthy vo...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章,多中心研究,随机对照试验
doi：10.1016/s0015-0282(00)00696-8
更新日期：2000-09-01 00:00:00
abstract：OBJECTIVE:To quantify nerve fibers and mast cells in human ovaries at different functional stages. DESIGN:Retrospective study. SETTING:Research laboratory of the university. SPECIMEN(S): 8 human ovaries in the follicular (cyclic) phase, 7 polycystic ovaries, and postmenopausal ovaries with (n=5) or without (n=7) hype...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(01)01805-2
更新日期：2001-06-01 00:00:00
abstract：:The morphologic characteristics of 85 cryptorchid and 63 contralateral (scrotal) testes showing histologic changes of puberty were studied. Spermatogenesis was not only delayed on the cryptorchid side but also hypocellular in all cases. The scrotal testes appeared hypocellular in 82.5% of the cases. Repetitive biopsie...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)46346-6
更新日期：1982-06-01 00:00:00
abstract：:The negative correlation between the thickness of the lamina propria and Johnsen's score count in individual seminiferous tubules was observed and the tubules with thin lamina propria indicated almost complete spermatogenesis. It is suggested that the thin lamina propria may be one of the good indicators of complete s...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2007.10.019
更新日期：2008-10-01 00:00:00
abstract：OBJECTIVE:To evaluate the effect of hypoxia on the production of vascular endothelial growth factor (VEGF) and soluble fms-like tyrosine kinase-1 (sFlt-1) in the human fallopian tube. DESIGN:The secretion of VEGF and sFlt-1 by cultured oviductal epithelial cells (OECs) and oviductal stromal fibroblasts (OSFs) in respo...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2005.10.023
更新日期：2006-04-01 00:00:00
abstract：OBJECTIVE:To determine whether the oncofetal protein IMP3 is detectable in endometriomas with or without histological atypia and whether IMP3 staining can be used as a triage tool to identify foci of atypical endometriosis in doubtful cases. DESIGN:Retrospective study. SETTING:Academic department and referral center ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2013.02.002
更新日期：2013-06-01 00:00:00
abstract：:Appropriate endometrial maturation is of paramount importance to achieve reproductive success. Practical and ethical considerations require that in vitro methods be available to evaluate regulation of human endometrial function. Additionally, tissue complexity requires separation of individual cell populations. This r...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)53160-4
更新日期：1989-12-01 00:00:00
abstract：OBJECTIVE:To study the efficacy of performing two inseminations per cycle in IUI with husband's sperm compared with one insemination per cycle. DESIGN:Meta-analysis. SETTING:Randomized and prospective trials comparing two inseminations vs. one insemination per cycle in IUI with husband's sperm, retrieved by MEDLINE a...
journal_title：Fertility and sterility
pub_type： 评论,杂志文章,meta分析,评审
doi：10.1016/j.fertnstert.2003.12.035
更新日期：2004-07-01 00:00:00
abstract：OBJECTIVE:To study the association of perinatal outcome and IVF transfer type in a group of infertility patients with standardized treatment and similar prognosis. DESIGN:Retrospective cohort study. SETTING:University-based infertility center, January 1998 to June 2006. PATIENT(S):Two hundred eighteen IVF pregnancie...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2010.05.049
更新日期：2011-02-01 00:00:00
abstract：OBJECTIVE:To evaluate surgical outcome and long-term follow-up of conservative laparoscopic management of urinary tract endometriosis (UTE). DESIGN:Prospective study. SETTING:Tertiary-care university hospital. PATIENT(S):Women with laparoscopic diagnosis and histologic confirmation of urinary bladder or ureteral end...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章
doi：10.1016/j.fertnstert.2009.04.019
更新日期：2010-08-01 00:00:00
abstract：:This report describes our initial experience with elective single blastocyst transfer in 19 patients who had a mean age of 36.3 +/- 2.4 years. The ongoing pregnancy rate, 53% after the fresh embryo transfer and 68% when thaw cycles are included, suggests that single blastocyst transfer has a place in this relatively o...
journal_title：Fertility and sterility
pub_type： 信件
doi：10.1016/j.fertnstert.2003.10.050
更新日期：2004-06-01 00:00:00
abstract：OBJECTIVE:To compare the FSH threshold level for follicle growth up to the preovulatory stage between superovulated and natural cycles. DESIGN:Normally cycling women were investigated during an untreated spontaneous cycle (control) and three cycles treated with FSH. SETTING:Infertility clinic in a university hospital...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)57610-9
更新日期：1995-06-01 00:00:00
abstract：OBJECTIVE:To evaluate spontaneous apoptosis in single-cell suspensions of eutopic and ectopic endometrium from women with endometriosis and in eutopic endometrium from fertile controls without endometriosis. DESIGN:Paired specimens of eutopic and ectopic endometrial tissue from patients with endometriosis and eutopic ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(98)00073-9
更新日期：1998-06-01 00:00:00
abstract：OBJECTIVE:To determine the expression of nitric oxide synthases (NOSs) and their modulation by hypoxia in human peritoneal (NF) and adhesion fibroblasts (ADF). DESIGN:Prospective experimental study. SETTING:University medical center. PATIENT(S):Fibroblasts from peritoneum and adhesion tissues. INTERVENTION(S):Hypox...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2007.07.1313
更新日期：2008-09-01 00:00:00
abstract：:We wished to verify whether semen processing by discontinuous double-layered density gradient centrifugation could improve sperm apoptotic DNA fragmentation rates using a commercially available deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling assay in 35 consecutive men presenting for ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2007.01.059
更新日期：2008-09-01 00:00:00
abstract：OBJECTIVE:To establish a mouse model for endometriosis and to evaluate roles of vascular endothelial growth factor (VEGF) and matrix metalloproteinase-2 (MMP-2) in the formation of disease. DESIGN:Experimental laboratory study. SETTING:A women's hospital in China. PATIENT(S) AND ANIMAL(S):Ten women with endometriosi...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2005.12.083
更新日期：2006-10-01 00:00:00
abstract：:A case of a genital tuberculosis found during laparoscopic evaluation for recurrent acute pelvic pain is presented. The symptoms of genital tuberculosis can vary greatly, and a laparoscopic approach is effective for making the diagnosis. Clinicians should consider tuberculosis as a differential diagnosis when encounte...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2007.09.063
更新日期：2008-06-01 00:00:00
abstract：OBJECTIVE:To compare saline infusion sonography (SIS) with gel instillation sonography (GIS) in terms of feasibility and diagnostic accuracy. DESIGN:Prospective cohort study. SETTING:Leuven University bleeding clinic. PATIENT(S):A total of 804 patients: two consecutive cohorts of 402 women undergoing SIS or GIS. IN...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章
doi：10.1016/j.fertnstert.2010.04.074
更新日期：2011-01-01 00:00:00
abstract：OBJECTIVE:To investigate a new role of interleukin (IL)-17A in endometriosis. DESIGN:Laboratory study. SETTING:University hospital. PATIENT(S):Patients with ovarian endometrioma undergoing laparoscopy or laparotomy. INTERVENTION(S):Primary culture of endometrioma stromal cells (EoSCs) was stimulated with IL-17A. Se...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2012.07.1117
更新日期：2012-11-01 00:00:00
abstract：OBJECTIVE:To review the literature to determine the magnitude of the effect of 150 micrograms desogestrel-30 micrograms ethinyl E2, an oral contraceptive (OC) formulation, on plasma lipid concentrations in healthy women using meta-analysis techniques. DATA SOURCES:All English-language published reports (1981 to 1991) ...
journal_title：Fertility and sterility
pub_type： 杂志文章,meta分析
doi：
更新日期：1996-06-01 00:00:00
abstract：OBJECTIVE:To evaluate how varicocele repair (VR) impacts pregnancy (PRs) and live birth rates in infertile couples undergoing assisted reproduction wherein the male partner has oligospermia or azoospermia and a history of varicocele. DESIGN:Systematic review and meta-analysis. SETTING:Not applicable. PATIENT(S):Azoo...
journal_title：Fertility and sterility
pub_type： 杂志文章,meta分析,评审
doi：10.1016/j.fertnstert.2016.07.1093
更新日期：2016-11-01 00:00:00
abstract：:Infertile men with azo- or oligospermia of unknown cause were investigated for evidence of testicular autoimmunity. Testicular germinal cell antibodies were found in 14% of the patients, compared with 5% of normal men, and 21% had spermatozoal antibodies, compared with 5% of the normal subjects. One-third had positive...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)41421-4
更新日期：1975-10-01 00:00:00
abstract：OBJECTIVE:To evaluate whether the efficacy of naltrexone administration in patients with hypothalamic amenorrhea correlates to the response to an acute naloxone test. DESIGN:Thirty patients with hypothalamic amenorrhea associated with weight loss were studied. After naloxone test (4 mg in bolus IV) patients were divid...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章
doi：10.1016/s0015-0282(16)57908-4
更新日期：1995-11-01 00:00:00
