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Interleukin-17A is present in neutrophils in endometrioma and stimulates the secretion of growth-regulated oncogene-α (Gro-α) from endometrioma stromal cells.

Abstract：

OBJECTIVE:To investigate a new role of interleukin (IL)-17A in endometriosis. DESIGN:Laboratory study. SETTING:University hospital. PATIENT(S):Patients with ovarian endometrioma undergoing laparoscopy or laparotomy. INTERVENTION(S):Primary culture of endometrioma stromal cells (EoSCs) was stimulated with IL-17A. Sections of endometrioma tissue were immunostained with antibodies for IL-17A, growth-regulated oncogene-α (Gro-α), and elastase, a marker of neutrophils. They were also examined with immunofluorescent double staining for IL-17A and myeloperoxidase, another marker of neutrophils. MAIN OUTCOME MEASURE(S):Concentration of Gro-α was measured using a specific ELISA. Neutrophil chemotaxis was measured with Boyden chamber method. Immunostained sections were examined under microscope. RESULT(S):Interleukin-17A increased the secretion of Gro-α from EoSCs dose-dependently. The conditioned medium of EoSCs stimulated with IL-17A attracted more neutrophils than that of EoSCs stimulated with vehicle, and the increase was inhibited by the addition of Gro-α-neutralizing antibody. On immunostaining, IL-17A and Gro-α were detected in similar areas of the stroma beneath the epithelium, where Gro-α was detected in cells with a stromal cell appearance whereas IL-17A was detected in neutrophils as determined by detection of elastase. Fluorescent immunostaining corroborated that myeloperoxidase-positive neutrophils were also positive for IL-17A. CONCLUSION(S):It is suggested that IL-17A produced by neutrophils stimulates Gro-α secretion from EoSCs, thereby recruiting more neutrophils and inducing perpetuating inflammation in endometriosis.

journal_name

Fertil Steril

journal_title

Fertility and sterility

authors

Takamura M,Osuga Y,Izumi G,Yoshino O,Koga K,Saito A,Hirata T,Hirota Y,Harada M,Hasegawa A,Taketani Y

doi

10.1016/j.fertnstert.2012.07.1117

subject

Has Abstract

pub_date

2012-11-01 00:00:00

pages

1218-24.e1-2

issue

eissn

0015-0282

issn

1556-5653

pii

S0015-0282(12)01870-5

journal_volume

pub_type

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Interleukin-17A is present in neutrophils in endometrioma and stimulates the secretion of growth-regulated oncogene-α (Gro-α) from endometrioma stromal cells.