Semen processing by density gradient centrifugation does not improve sperm apoptotic deoxyribonucleic acid fragmentation rates.

Abstract:

:We wished to verify whether semen processing by discontinuous double-layered density gradient centrifugation could improve sperm apoptotic DNA fragmentation rates using a commercially available deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling assay in 35 consecutive men presenting for assisted reproductive treatments. Although sperm motility did improve as expected, no effects were observed in sperm apoptotic DNA fragmentation rates, and this should be considered in the routine assisted reproduction setting.

journal_name

Fertil Steril

journal_title

Fertility and sterility

authors

Stevanato J,Bertolla RP,Barradas V,Spaine DM,Cedenho AP,Ortiz V

doi

10.1016/j.fertnstert.2007.01.059

subject

Has Abstract

pub_date

2008-09-01 00:00:00

pages

889-90

issue

3

eissn

0015-0282

issn

1556-5653

pii

S0015-0282(07)00220-8

journal_volume

90

pub_type

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