Abstract：
OBJECTIVE:To report a live birth after the transfer of a single blastocyst derived from a human oocyte cryopreserved for 5 years. DESIGN:Case report. SETTING:Private assisted reproduction center. PATIENT(S):A 39-year-old woman with tubal infertility and her partner with male-related infertility. INTERVENTION(S):Oocyte cryopreservation (in 1.5 mol/L 1,2-propanediol and 0.3 mol/L sucrose by slow freezing-rapid thawing protocol) when the patient was 34 years old. Oocyte thawing after 5 years of cryostorage. Insemination by intracytoplasmic sperm injection of the three best surviving oocytes, according to the Italian law regulating assisted reproductive technology. MAIN OUTCOME MEASURE(S):Oocyte survival after thawing. Fertilization, cleavage, and embryo development into blastocyst stage. RESULT(S):Four of six mature (metaphase II) frozen oocytes survived after thawing. Three of them were injected: these three oocytes were fertilized, and one developed into a five-cell embryo on day 3. This embryo developed into blastocyst and was transferred on day 6. A healthy female neonate weighing 3,410 g was born. CONCLUSION(S):After 5 years of storage in liquid nitrogen, cryopreserved oocytes with a slow cooling-rapid thawing protocol can develop in vitro to blastocyst stage and produce a live birth.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Parmegiani L,Fabbri R,Cognigni GE,Bernardi S,Pocognoli P,Filicori Mdoi
10.1016/j.fertnstert.2008.01.106subject
Has Abstractpub_date
2008-11-01 00:00:00pages
2014.e7-10issue
5eissn
0015-0282issn
1556-5653pii
S0015-0282(08)00231-8journal_volume
90pub_type
杂志文章abstract：OBJECTIVE:To determine the feasibility of performing in vivo magnetic resonance elastography (MRE) for uterine leiomyoma. DESIGN:Pilot study. SETTING:Academic medical center. PATIENT(S):Six subjects planning surgical excision of uterine leiomyomas. INTERVENTION(S):MRE before planned surgery. MAIN OUTCOME MEASURE(S...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章
doi：10.1016/j.fertnstert.2010.06.004
更新日期：2011-01-01 00:00:00
abstract：:This study was undertaken to determine whether women with endometriosis have altered protein, progesterone (P), and protease inhibitor concentrations in their uterine fluid and peritoneal fluid (PF) compared with controls at different phases of the menstrual cycle. Uterine flushings (UFs), PF, and blood were obtained ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)49994-2
更新日期：1987-02-01 00:00:00
abstract：OBJECTIVE:To evaluate expression of interleukin-1 receptor type I in the endometrium of fertile women throughout the menstrual cycle and to investigate whether unexplained infertility may be associated with abnormal expression of IL-1 receptor type I. DESIGN:Retrospective study using immunohistochemical technique, Wes...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(00)01634-4
更新日期：2001-01-01 00:00:00
abstract：OBJECTIVE:To evaluate the pregnancy and delivery outcome of robot-assisted tubal reanastomosis. DESIGN:Retrospective cohort study. SETTING:University hospital. PATIENT(S):Ninety-seven patients with available follow-up who underwent the reversal of tubal ligation, with a median age of 37 years (range, 24-47 years). ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2009.10.028
更新日期：2010-10-01 00:00:00
abstract：OBJECTIVE:To explore the role of tumor necrosis factor (TNF) α, an early embryonic product, on endometrial epithelial cell migration and endometrial milk fat globule-epidermal growth factor 8 protein (MFG-E8) production. DESIGN:In vitro study. SETTING:Academic center. INTERVENTION(S):Ishikawa cells, used as surrogat...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2013.10.032
更新日期：2014-02-01 00:00:00
abstract：OBJECTIVE:To evaluate tubal morphology, trophoblast proliferation, and inflammatory reaction in response to methotrexate (MTX) treatment of ectopic pregnancy (EP). DESIGN:Nonrandomized controlled study. SETTING:Academic hospital. PATIENTS:Archival specimens from 10 EP unsuccessfully treated with MTX and 10 cases pri...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章
doi：
更新日期：1996-04-01 00:00:00
abstract：OBJECTIVE:To determine the effects of tibolone and continuous combined HRT (ccHRT) on parameters in the clotting cascade. DESIGN:Randomized, double-blind study. SETTING:Hemostasis unit of a university hospital clinic in Germany. PATIENT(S):Sixty healthy postmenopausal women. INTERVENTION(S):Twenty-nine subjects wer...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章,多中心研究,随机对照试验
doi：10.1016/s0015-0282(00)00587-2
更新日期：2000-07-01 00:00:00
abstract：OBJECTIVE:The present study examines the safety, effectiveness, and local tissue response for a new transcervical fallopian tube permanent contraceptive device, the STOP device (Conceptus, Inc., San Carlos, CA). DESIGN:Nonrandomized prospective evaluation of tubal occlusion and histologic response. SETTING:Inpatient,...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(01)02858-8
更新日期：2001-11-01 00:00:00
abstract：OBJECTIVE:To assess the endocrine and clinical responses to microdose GnRH agonist (GnRH-a) that was administered in the early follicular phase before controlled ovarian hyperstimulation to poor responders who were candidates for IVF-ET. DESIGN:Prospective nonrandomized trial with historical controls. SETTING:Tertiar...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(97)00575-x
更新日期：1998-03-01 00:00:00
abstract：:The role of maternal diabetes as a cause of repetitive early pregnancy loss was evaluated by examining the reproductive performance of 154 diabetic patients in 489 pregnancies. The overall incidence of spontaneous abortion among gestational diabetics (12.7%) and frank diabetics (12.2%) was not significantly different ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)45797-3
更新日期：1981-10-01 00:00:00
abstract：OBJECTIVE:To determine whether there is an association between abnormal semen parameters and occupational exposures to organic solvents, metals, and pesticides. DESIGN:Case-control study using three case groups based on different cutoff values for semen parameters and one standard reference group. SETTING:University ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(98)00542-1
更新日期：1999-04-01 00:00:00
abstract：OBJECTIVE:To evaluate vaginal bleeding profiles with lower doses of conjugated equine estrogens (CEE) and medroxyprogesterone acetate (MPA) as continuous combined therapy. DESIGN:The Women's Health, Osteoporosis, Progestin, Estrogen (Women's HOPE) study, a randomized, double-blind, placebo-controlled trial. SETTING:S...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章,多中心研究,随机对照试验
doi：10.1016/s0015-0282(01)01792-7
更新日期：2001-06-01 00:00:00
abstract：OBJECTIVE:To determine the levels of postacrosomal WW binding protein (PAWP) in the spermatozoa of men that were used clinically for intracytoplasmic sperm injection (ICSI) and to correlate them with infertility treatment outcomes. DESIGN:Prospective clinical and laboratory study. SETTING:University-based laboratory ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2014.05.003
更新日期：2014-08-01 00:00:00
abstract：:The human placenta in vitro releases luteinizing hormone-releasing factor (LRF) which is immunologically indistinguishable from hypothalamic LRF. The release of LRF by placental tissue increased during the 4 days of culture and exceeded the original tissue content more than 5-fold. These data suggest that the human pl...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)44236-6
更新日期：1979-09-01 00:00:00
abstract：:Estrogen (E)/progestin therapy for functional ovarian cysts is widely used in clinical practice, but the efficacy of this treatment has not been determined in controlled trials. In this study, we examined the effect of E/progestin administration in a group of infertility patients enrolled in a program of ovulation ind...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章,随机对照试验
doi：
更新日期：1990-11-01 00:00:00
abstract：:Diagnostic laparoscopy is commonly performed on patients as part of a complete infertility investigation. Recently published protocols have investigated the efficacy of using empiric ovulation induction, intrauterine insemination, or both before beginning in vitro fertilization. Because many patients enrolled in these...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)60374-6
更新日期：1988-12-01 00:00:00
abstract：OBJECTIVE:To investigate the molecular mechanism for declining sperm motility in older men. DESIGN:Retrospective and prospective study. SETTING:University-based andrology unit. PATIENT(S):Semen was collected from 2,111 patients and sperm donors. INTERVENTION(S):None. MAIN OUTCOME MEASURE(S):Age, sperm concentratio...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2005.05.020
更新日期：2005-11-01 00:00:00
abstract：OBJECTIVE:To test the hypothesis that adrenal androgen (AA) secretion is an inherited trait in PCOS and that serum DHEAS concentrations, as a marker of AA secretion, will be correlated between women with PCOS and their sisters. DESIGN:Prospective case-control. SETTING:Tertiary care center. PATIENT(S):Sixty-two PCOS ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2006.05.045
更新日期：2006-12-01 00:00:00
abstract：:This study investigated the prevalence of Tyr204Phe and Val34Leu polymorphisms in two Brazilian ethnic groups (171 Caucasians and 27 Blacks, and 117 men and 81 women) and in patients with recurrent miscarriages (RM) (86 women: 53 Caucasians and 33 Blacks). Study groups were matched to control groups by race and age. T...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2004.04.052
更新日期：2004-11-01 00:00:00
abstract：OBJECTIVE:To determine the relative effectiveness of single vs. two transcervical monthly insertions of 252 mg of quinacrine for female sterilization. DESIGN:Controlled clinical study. SETTING:Family planning clinics of 6 academic centers. PATIENT(S):Sexually active reproductive-age women requesting sterilization. ...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0015-0282(02)02957-6
更新日期：2002-05-01 00:00:00
abstract：:Hormonal profiles were compared in 14 ovulatory women who were treated with two different doses of gonadotropins in successive in vitro fertilization cycles. All patients suffered from mechanical causes of infertility. Serum estradiol (E2), follicle-stimulating hormone (FSH), luteinizing hormone (LH), and progesterone...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1988-06-01 00:00:00
abstract：:A 30-year-old nulliparous woman, who was infertile for greater than 3 years, conceived after the procedures of a simple method of zona cutting, cryopreservation, and ZIFT. This case illustrates: (1) acid medium, chymotrypsin, or sucrose are not needed for the procedure of zona cutting; (2) the zygotes resulting from z...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)54139-9
更新日期：1991-02-01 00:00:00
abstract：:Effects of single subcutaneous doses (1, 5, 20, and 100 micrograms) of nafarelin, a potent gonadotropin-releasing hormone agonist, on the physiologic events of the human menstrual cycle were studied in 28 normal women. Nafarelin entered the circulation rapidly after injection. Peak concentrations were observed within ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)48432-3
更新日期：1985-03-01 00:00:00
abstract：OBJECTIVE:To evaluate, using the hemizona assay (HZA), whether egg yolk treatment of human sperm enhances binding to the human zona pellucida in vitro and to determine whether such a treatment is as efficient as the standard swim-up procedure for promoting sperm binding ability. DESIGN:Ejaculates were divided into ali...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)55261-3
更新日期：1992-09-01 00:00:00
abstract：OBJECTIVE:To determine whether cryptorchidism associated with azoospermia or oligozoospermia may be due to microdeletions of the Y chromosome. DESIGN:Controlled clinical study. SETTINGS:Division of Medical Genetics and the Andrology Centre, Department of Obstetrics and Gynecology, University Medical Center, Ljubljana...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(03)00375-3
更新日期：2003-06-01 00:00:00
abstract：:The hormonal profile of the aging male reveals an associated decrease in free testosterone and 5 alpha-dihydrotestosterone (DHT) levels and increased luteinizing hormone levels. Later events consist of a decrease in total testosterone, stable DHT, and increased follicle-stimulating hormone and estradiol levels. Althou...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)41958-8
更新日期：1976-07-01 00:00:00
abstract：:Forty-seven patients at high risk for ovarian hyperstimulation syndrome because of markedly elevated serum E2 levels on either long-luteal or microdose flare leuprolide acetate regimens were treated with ganirelix acetate. Despite being pretreated with GnRH agonist and without withholding gonadotropins, serum E2 decre...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2005.07.1291
更新日期：2006-01-01 00:00:00
abstract：:This study fails to uphold the concept of invisible, microscopic endometriosis. Peritoneum devoid of disease exists, can be described, and can be consistently found. ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)60205-4
更新日期：1988-10-01 00:00:00
abstract：OBJECTIVE:To analyze sonographically the endometrium in patients undergoing controlled ovarian stimulation with menotropins in order to determine the significance of endometrial pattern and thickness on pregnancy rate. DESIGN:This is a prospective, nonrandomized study comparing pregnancy rates in patients with hyperec...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章
doi：10.1016/s0015-0282(16)58447-7
更新日期：1996-08-01 00:00:00
abstract：:The result of the hormone concentration of one blood sample was used to determine the accuracy of predicting not only the hormone concentrations of a second and third sample drawn 15 minutes apart, but also the mean value of the three samples. Three blood specimens from 73 men involved in two previously reported studi...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)59662-9
更新日期：1988-01-01 00:00:00
