In vitro fertilization outcomes after intracytoplasmic sperm injection with fresh or frozen-thawed testicular spermatozoa.


OBJECTIVE:To compare the outcomes of intracytoplasmic sperm injection (ICSI) and in vitro fertilization (IVF) with fresh and cryopreserved testicular spermatozoa in patients with obstructive and nonobstructive azoospermia. DESIGN:Retrospective analysis of consecutive ICSI cycles. SETTING:Large urban reproductive medicine program. PATIENT(S):Twenty-nine patients with obstructive and nonobstructive azoospermia undergoing testicular sperm extraction for a total of 46 IVF-ICSI cycles (12 fresh, 34 frozen). INTERVENTION(S):Testicular sperm extraction, cryopreservation, and IVF-ICSI with fresh or frozen-thawed spermatozoa. MAIN OUTCOME MEASURE(S):Fertilization rates, embryo cleavage rates, embryo implantation rates, clinical pregnancy rates per cycle and per embryo transfer, and delivery and spontaneous abortion rates. RESULT(S):No statistically significant differences were noted in any of the parameters examined between IVF-ICSI cycles from fresh or frozen-thawed testicular spermatozoa. Fertilization rates were 56% with fresh vs. 61% with frozen-thawed testicular sperm, cleavage rates 92% vs. 95%, implantation rates 26% vs. 17%, clinical pregnancy rates per cycle 33% vs. 41%, and pregnancy rates per embryo transfer 33% vs. 45%, respectively. Delivery rates were 75% with fresh vs. 69.2% with frozen-thawed testicular sperm, and spontaneous abortion rates 25% and 30.8%, respectively. CONCLUSION(S):No differences were found in IVF-ICSI outcomes between cryopreserved and fresh testicular sperm. In addition, cryopreservation provides several advantages for the patients and reproductive team.


Fertil Steril


Fertility and sterility


Habermann H,Seo R,Cieslak J,Niederberger C,Prins GS,Ross L





Has Abstract


2000-05-01 00:00:00














  • Do variations in assisted reproductive technology programs' screening practices indicate a need for national guidelines? Another perspective.

    abstract::Some see an "alarming inconsistency" in candidate-screening practices of assisted reproductive technology (ART) programs and call for common guidelines. However, reasonable people can disagree about who should be prevented from having children and why. ...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Steinbock B

    更新日期:2005-11-01 00:00:00

  • Correlation between fetal heart rate, crown-rump length, and beta-human chorionic gonadotropin levels during the first trimester of well-timed conceptions resulting from infertility treatment.

    abstract:OBJECTIVES:To evaluate sequential changes in fetal heart rate (FHR) during the first trimester of well-timed pregnancies resulting from infertility treatment and to correlate the data with fetal growth curve and beta-hCG levels. DESIGN:Serial measurements of FHR, crown-rump length (CRL) and beta-hCG at weekly interval...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Rotsztejn D,Rana N,Dmowski WP

    更新日期:1993-06-01 00:00:00

  • Mitochondrial activation in the growth-restricted fetus of monochorionic twins.

    abstract:OBJECTIVE:To study the regulatory mechanisms of selective intrauterine growth restriction (sIUGR) independent of confounding genetic factors, monochorionic (MC) twins are the ideal model, because they have identical genomic DNA. We hypothesize that the intrauterine growth restriction fetus has mitochondrial activation ...

    journal_title:Fertility and sterility

    pub_type: 杂志文章,随机对照试验


    authors: Chang YL,Wang CN,Wei PC,Peng HH,Chao AS,Chang SD,Cheng PJ,Wang TH

    更新日期:2013-07-01 00:00:00

  • Endometrioma-related reduction in ovarian reserve (ERROR): a prospective longitudinal study.

    abstract:OBJECTIVE:To evaluate whether endometrioma is associated with a progressive decline in ovarian reserve, and to compare the rate of decline with natural decline in ovarian reserve. DESIGN:Prospective, observational study. SETTING:Tertiary university hospital, endometriosis clinic. PATIENT(S):Forty women with endometr...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Kasapoglu I,Ata B,Uyaniklar O,Seyhan A,Orhan A,Yildiz Oguz S,Uncu G

    更新日期:2018-07-01 00:00:00

  • Advanced surgical sperm recovery is a viable option for intracytoplasmic sperm injection in patients with obstructive or nonobstructive azoospermia.

    abstract:OBJECTIVE:To determine whether advanced sperm retrieval is appropriate in cases of obstructive and nonobstructive azoospermia. DESIGN:Prospective controlled study. SETTING:Tertiary care center. PATIENT(S):Men with obstructive and nonobstructive azoospermia, and their partners. INTERVENTION(S):Surgical sperm retriev...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Jaroudi K,Coskun S,Hollanders J,Al-Hassan S,Al-Sufayan H,Atared A,Merdad T

    更新日期:1999-09-01 00:00:00

  • Treatment of uterine leiomyomata with a luteinizing hormone-releasing hormone agonist: the possibility of nonsurgical management in selected perimenopausal women.

    abstract:OBJECTIVE:To evaluate the efficacy of luteinizing hormone-releasing hormone agonist (LH-RH-a) in the treatment of leiomyomata. DESIGN:A retrospective randomized trial. SETTING:Hospital department of obstetrics and gynecology. PATIENTS:Twenty-five women, ages 36 to 54 years with symptomatic uterine leiomyomata, were ...

    journal_title:Fertility and sterility

    pub_type: 临床试验,杂志文章


    authors: Nakamura Y,Yoshimura Y,Yamada H,Ubukata Y,Ando M,Suzuki M

    更新日期:1991-05-01 00:00:00

  • Pregnancy rate is significantly higher in in vitro fertilization procedure with spermatozoa isolated from nonliquefying semen in which liquefaction is induced by alpha-amylase.

    abstract::In 115 patients (group I) in which normal liquefaction of semen occurred, the fertilization rate of the yielded oocytes was 71.2% after standard in vitro fertilization procedure, and the viable pregnancy rate per patient per cycle was 13.0%. In a comparable group of 26 patients (group II), in which semen liquefaction ...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Vermeiden JP,Bernardus RE,ten Brug CS,Statema-Lohmeijer CH,Willemsen-Brugma AM,Schoemaker J

    更新日期:1989-01-01 00:00:00

  • Treatment of hirsutism with ethinyl estradiol-desogestrel contraceptive pills has beneficial effects on the lipid profile and improves insulin sensitivity.

    abstract:OBJECTIVE:To evaluate the effects on the lipid pattern and insulin sensitivity of hirsute women of an oral contraceptive pill containing 30 microg of ethinyl estradiol and 150 microg of desogestrel. DESIGN:Prospective clinical study. SETTING:Tertiary care institutional hospital. PATIENT(S):16 hirsute women. INTERVE...

    journal_title:Fertility and sterility

    pub_type: 临床试验,杂志文章


    authors: Escobar-Morreale HF,Lasunción MA,Sancho J

    更新日期:2000-10-01 00:00:00

  • Absence of block to polyspermy at the human oolemma.

    abstract:OBJECTIVE:To study the fusiogenic ability of human spermatozoa and oocytes. DESIGN:Retrospective study of 3,027 oocytes included in a program of subzonal insemination (SUZI). SETTING:Assisted fertilization program in an academic research environment. PATIENT(S):Couples with characterized male factor infertility or p...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Wolf JP,Ducot B,Aymar C,Rodrigues D,Desjardin S,Jardin A,Jouannet P

    更新日期:1997-06-01 00:00:00

  • A randomized, placebo-controlled, double-blind study evaluating leuprolide acetate depot treatment before myomectomy.

    abstract::Eighteen premenopausal women with symptomatic leiomyomata uteri were enrolled in a stratified, randomized, double-blind, placebo-controlled study evaluating the efficacy of leuprolide acetate (LA) depot treatment before myomectomy. Stratification was based on pretreatment uterine volume (less than 600 cm3 versus great...

    journal_title:Fertility and sterility

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Friedman AJ,Rein MS,Harrison-Atlas D,Garfield JM,Doubilet PM

    更新日期:1989-11-01 00:00:00

  • Ultrasonographically determined size of seminiferous tubules predicts sperm retrieval by microdissection testicular sperm extraction in men with nonobstructive azoospermia.

    abstract:OBJECTIVE:To investigate the value of the ultrasonographically determined size of seminiferous tubules and other conventional parameters for predicting sperm retrieval by microdissection testicular sperm extraction (micro-TESE). DESIGN:Clinical retrospective study. SETTING:Two urological clinics. PATIENT(S):Eight hu...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Nariyoshi S,Nakano K,Sukegawa G,Sho T,Tsuji Y

    更新日期:2020-01-01 00:00:00

  • The antiprogesterone Org 31710 inhibits human blastocyst-endometrial interactions in vitro.

    abstract:OBJECTIVE:To investigate the effect of the anti-P Org 31710 on human blastocyst attachment to cultured endometrial epithelial cells. DESIGN:Experimental in vitro study. SETTING:University hospital. PATIENT(S):Eleven fertile endometrial donors. INTERVENTION(S):Timed endometrial biopsy for cell cultures. MAIN OUTCOM...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Petersen A,Bentin-Ley U,Ravn V,Qvortrup K,Sørensen S,Islin H,Sjögren A,Mosselmann S,Hamberger L

    更新日期:2005-04-01 00:00:00

  • Factors affecting sperm motility. V. Washing and resuspension of human spermatozoa in various artificial media.

    abstract::Fresh semen specimens from fertile donors were subjected to one-step and two-step washings in six various commonly used artificial media. It was found that washing procedures per se, in most cases, had an immediate and extended harmful effect on sperm motility which was much more prominent after the second washing. Ho...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Makler A,Jakobi P

    更新日期:1981-04-01 00:00:00

  • Congenital anomalies and assisted reproductive technology: more of the same.

    abstract::Klemetti's birth registry study is put in context of other major studies. Summary advice is offered to practitioners as to what they should tell parents who conceive with assisted reproductive technology. ...

    journal_title:Fertility and sterility

    pub_type: 评论,信件


    authors: Sutcliffe AG

    更新日期:2005-11-01 00:00:00

  • Optimal timing of oocyte maturation and its relationship with the spindle assembly and developmental competence of in vitro matured human oocytes.

    abstract:OBJECTIVE:To develop and test a simple, feasible approach to improve spindle assembly and developmental competence of human in vitro matured oocytes by parthenogenetic activation in maturation medium. DESIGN:Prospective, randomized study. SETTING:University research laboratory-based assisted reproductive technology l...

    journal_title:Fertility and sterility

    pub_type: 杂志文章,随机对照试验


    authors: Yu Y,Yan J,Liu ZC,Yan LY,Li M,Zhou Q,Qiao J

    更新日期:2011-07-01 00:00:00

  • Effects of aging on menstrual cycle hormones and endometrial maturation.

    abstract:OBJECTIVE:To investigate changes in menstrual cycle hormones and endometrial maturation that may contribute to the decline in fertility with aging. DESIGN:Prospective controlled clinical study. SETTING:Normal human volunteers in an academic research institution. SUBJECTS:Women with regular menstrual cycles. INTERVE...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Batista MC,Cartledge TP,Zellmer AW,Merino MJ,Axiotis C,Bremner WJ,Nieman LK

    更新日期:1995-09-01 00:00:00

  • Effects of ethinyl estradiol on semen quality and various hormonal parameters in a eugonadal male.

    abstract:OBJECTIVE:To determine the influence of estrogens on male fertility. DESIGN:A 36-year-old eugonadal male was subjected to two different regimens of treatment with ethinyl estradiol (EE2). Sperm quality, immunoreactive luteinizing hormone (LH) and follicle-stimulating Hormone (FSH), testosterone (T), estrone (E1), estr...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Lübbert H,Leo-Rossberg I,Hammerstein J

    更新日期:1992-09-01 00:00:00

  • Effects of fibrin sealant on single-layer uterine incision closure in the New Zealand white rabbit.

    abstract:OBJECTIVE:To determine if the addition of fibrin sealant to incision closure in a rabbit uterine horn myomectomy model affects adhesion formation or strength of incision closure. DESIGN:Prospective randomized controlled trial. SETTING:Academic research center. ANIMAL(S):New Zealand white female rabbits. INTERVENTIO...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Peacock KE,Hurst BS,Marshburn PB,Matthews ML

    更新日期:2006-04-01 00:00:00

  • Inhibitory effect of interferon and tumor necrosis factor on human luteal function in vitro.

    abstract:OBJECTIVE:To investigate whether immunological mechanisms may be involved in human luteal function. DESIGN:The effects of the cytokines, interferon-alpha (IFN-alpha), interferon-gamma (IFN-gamma) and tumor necrosis factor alpha (TNF-alpha) on steroidogenesis by human luteal cells were examined in vitro. The dispersed ...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Wang HZ,Lu SH,Han XJ,Zhou W,Sheng WX,Sun ZD,Gong YT

    更新日期:1992-11-01 00:00:00

  • Modified natural-cycle in vitro fertilization should be considered as the first approach in young poor responders.

    abstract::The use of modified natural-cycle in vitro fertilization (IVF) is a valuable alternative to controlled ovarian hyperstimulation in young poor responders and should be considered in patients who require IVF and demonstrate endocrinologic evidence of ovarian aging and in those who have had one or two canceled controlled...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Kadoch IJ,Phillips SJ,Bissonnette F

    更新日期:2011-11-01 00:00:00

  • Variation in antimüllerian hormone concentration during the menstrual cycle may change the clinical classification of the ovarian response.

    abstract:OBJECTIVE:To assess the variability of antimüllerian hormone (AMH) concentrations in women with "adequate ovarian reserve" during unstimulated menstrual cycles and to determine the impact on clinical classifications. DESIGN:Pilot cohort study. SETTING:Private fertility clinic. PATIENT(S):Twelve consecutive women (ag...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Hadlow N,Longhurst K,McClements A,Natalwala J,Brown SJ,Matson PL

    更新日期:2013-05-01 00:00:00

  • A prospective, randomized study comparing laparoscopic ovarian cystectomy versus fenestration and coagulation in patients with endometriomas.

    abstract:OBJECTIVE:To determine the difference between two laparoscopic methods for the management of endometriomas with regard to recurrence of signs and symptoms and pregnancy rate. DESIGN:Prospective, randomized clinical trial. SETTING:Infertility and gynecologic endoscopy units of two medical university hospitals. PATIEN...

    journal_title:Fertility and sterility

    pub_type: 临床试验,杂志文章,多中心研究,随机对照试验


    authors: Alborzi S,Momtahan M,Parsanezhad ME,Dehbashi S,Zolghadri J,Alborzi S

    更新日期:2004-12-01 00:00:00

  • Specific isoforms of leucine-rich alpha2-glycoprotein detected in the proliferative endometrium of women undergoing assisted reproduction are associated with spontaneous pregnancy.

    abstract:OBJECTIVE:To examine differences in specific protein expression from the surface of the human endometrium with respect to eventual pregnancy in infertile women. DESIGN:Laboratory study. SETTING:University hospital. PATIENT(S):Thirty-one women presenting for investigation into infertility at an assisted reproductive ...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Gillott DJ,Al-Rumaih HM,Leung KY,Eldib A,Grudzinskas JG

    更新日期:2008-09-01 00:00:00

  • The relation of fertility and ovarian histology after bilateral ovarian wedge resection.

    abstract::The immediate and long term fertility after bilateral ovarian wedge resection in 53 women with clomiphene citrate-resistant hyperandrogenic chronic anovulation is related to the histology of the ovarian wedges. Patients with polycystic ovaries and hyperplastic stromal abnormalities had most spontaneous conceptions and...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Lappöhn RE,Bogchelman DH

    更新日期:1989-08-01 00:00:00

  • Impact of reproductive history on in vitro fertilization and intracytoplasmic sperm injection outcome: evidence from the German IVF Registry.

    abstract:OBJECTIVE:To evaluate the effect of reproductive history on the outcome of different procedures in assisted reproductive technologies (ART) comparing IVF, ICSI, and cryopreserved embryo transfer (CPE). DESIGN:Prospective registration of ART cycles and their outcomes. SETTING:One hundred three reproductive programs in...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Kupka MS,Dorn C,Richter O,Felberbaum R,van der Ven H

    更新日期:2003-09-01 00:00:00

  • Preimplantation genetic diagnosis increases the implantation rate in human in vitro fertilization by avoiding the transfer of chromosomally abnormal embryos.

    abstract:OBJECTIVE:To verify the percentage of chromosomally abnormal preimplantation embryos in patients with a poor prognosis and possibly to increase the chance of implantation by selecting chromosomally normal embryos. DESIGN:A prospective, randomized, controlled study. SETTING:In vitro fertilization program at the Reprod...

    journal_title:Fertility and sterility

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Gianaroli L,Magli MC,Ferraretti AP,Fiorentino A,Garrisi J,Munné S

    更新日期:1997-12-01 00:00:00

  • Clomiphene citrate intrauterine insemination (IUI) before gonadotropin IUI affects the pregnancy rate and the rate of high-order multiple pregnancies.

    abstract:OBJECTIVE:To determine if previous treatment with clomiphene citrate intrauterine insemination (CC-IUI) affects pregnancy and high-order multiple pregnancy (HOMP) rates in subsequent hMG-IUI or FSH-IUI cycles. DESIGN:Retrospective cohort study. SETTING:Private infertility clinic. PATIENT(S):Five hundred fifty-one pa...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Dickey RP,Taylor SN,Lu PY,Sartor BM,Pyrzak R

    更新日期:2004-03-01 00:00:00

  • Pubertal development of the first cohort of young adults conceived by in vitro fertilization in the United States.

    abstract:OBJECTIVE:To characterize pubertal development of the first generation of young adults born as a result of in vitro fertilization (IVF). Demographic, clinical, and body size characteristics were examined regarding developmental milestones. DESIGN:Cross-sectional. SETTING:Academic center. PATIENT(S):Young adults (18-...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Beydoun HA,Sicignano N,Beydoun MA,Bocca S,Stadtmauer L,Oehninger S

    更新日期:2011-02-01 00:00:00

  • Association of human leukocyte antigen-G 14 bp polymorphism with recurrent pregnancy loss in European countries: a meta-analysis of literature studies.

    abstract:OBJECTIVE:To study the controversial association between human leukocyte antigen-G (HLA-G) 14 bp polymorphism and recurrent pregnancy loss (RPL). We performed a meta-analysis of studies in the literature that enrolled only women of European countries who experienced RPL spontaneously or after undergoing IVF. DESIGN:Sy...

    journal_title:Fertility and sterility

    pub_type: 杂志文章,meta分析


    authors: Monti M,Lupoli R,Sosa Fernandez LM,Cirillo F,Di Minno MND

    更新日期:2019-09-01 00:00:00

  • Stromal cells from endometriotic lesions and endometrium from women with endometriosis have reduced decidualization capacity.

    abstract:OBJECTIVE:To evaluate the phenotype, proliferative, and differentiation capacities in vitro of stromal cells derived from peritoneal, ovarian, and deeply infiltrating endometriosis. DESIGN:Experimental study using phase contrast microscopy, immunocytochemistry, and functional bioassays. SETTING:University-based labor...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Klemmt PA,Carver JG,Kennedy SH,Koninckx PR,Mardon HJ

    更新日期:2006-03-01 00:00:00