Abstract：
OBJECTIVE:To evaluate human sperm acrosomal status, zona pellucida (ZP)-binding capacity, and sperm motion characteristics after treatment with pertussis toxin followed by exposure to increasing concentrations of solubilized human ZP. DESIGN:Prospective analytical study. SETTING:Normal human sperm donors in an academic research environment. INTERVENTION:Sperm were prepared with a wash and swim-up method and treated with a final concentration of 100 ng/mL pertussis toxin. Acrosomal status were determined using a Pisum sativum agglutinin-fluorescien-isothiocyanate method after exposure of sperm to 0.25, 0.5, 0.75, and 1.00 ZP/microL solutions of human ZP. Zona binding potential was recorded using intact zona-binding assays. Motion characteristics were recorded with a semen analyzer. MAIN OUTCOME MEASURE:Percentage acrosome-reacted sperm, number of zona-bound sperm, and sperm motion parameters. RESULTS:Spermatozoa treated with 100 ng/mL pertussis toxin, followed by ZP-mediated acrosome reaction induction, showed a significant decrease in the percentage of acrosome-reacted sperm compared with untreated controls. Motion characteristics of 3-hour capacitated sperm after treatment with either phosphate-buffered saline (PBS) or pertussis toxin were not different. Pertussis toxin-treated sperm populations bound significantly more sperm to the ZP after 4 hours incubation compared with the PBS-control groups: 137.1 +/- 8.0 compared with 96.3 +/- 7.0 (mean +/- SEM). CONCLUSIONS:The data support the concept of the controlling mechanism and importance of G proteins during the ZP-mediated acrosome reaction. Intact acrosome correlate with and are needed to ensure tight zona binding.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Franken DR,Morales PJ,Habenicht UFsubject
Has Abstractpub_date
1996-12-01 00:00:00pages
1009-11issue
6eissn
0015-0282issn
1556-5653pii
S0015-0282(16)58698-1journal_volume
66pub_type
杂志文章abstract：OBJECTIVE:To determine whether retinoic acid (RA) can regulate the expression of interleukin (IL)-6 in human endometrial cells in a manner that might be beneficial to women with endometriosis. DESIGN:In vitro study. SETTING:Academic medical center. PATIENT(S):Patients with endometriosis and controls. INTERVENTION(S...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(00)00483-0
更新日期：2000-05-01 00:00:00
abstract：OBJECTIVE:To evaluate vaginal bleeding profiles with lower doses of conjugated equine estrogens (CEE) and medroxyprogesterone acetate (MPA) as continuous combined therapy. DESIGN:The Women's Health, Osteoporosis, Progestin, Estrogen (Women's HOPE) study, a randomized, double-blind, placebo-controlled trial. SETTING:S...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章,多中心研究,随机对照试验
doi：10.1016/s0015-0282(01)01792-7
更新日期：2001-06-01 00:00:00
abstract：OBJECTIVE:To evaluate the effect of melatonin on cyclophosphamide (CP)- and cisplatin-induced testicular toxicity with use of sperm parameters and biochemical and histopathologic approaches. DESIGN:Experimental study. SETTING:Vakif Gureba Hospital, Istanbul, Turkey. ANIMALS:Six-week-old adult male Wistar albino rats...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2008.07.1758
更新日期：2009-09-01 00:00:00
abstract：OBJECTIVES:To evaluate sequential changes in fetal heart rate (FHR) during the first trimester of well-timed pregnancies resulting from infertility treatment and to correlate the data with fetal growth curve and beta-hCG levels. DESIGN:Serial measurements of FHR, crown-rump length (CRL) and beta-hCG at weekly interval...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)55971-8
更新日期：1993-06-01 00:00:00
abstract：:Human testes, removed in treating patients for prostatic carcinoma, were used. Tissues were homogenized and incubated in vitro with labeled progesterone and 2 different antiovulatories, lynestrenol (17alpha-ethin yl-17beta-hydroxy-estr-4-one) and MK 665 (17alpha-chloroethinyl-19-nor-4 ,9(10)-androstadiene-17beta-ol-...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)36309-9
更新日期：1967-05-01 00:00:00
abstract：:Twelve women of normal weight (ages 17 to 36 years) with scanty menstrual bleeding were studied. They had no signs of virilization, gynecologic or endocrine pathology, or past history of hormonal treatment. Five women (group 1) experienced withdrawal bleeding after a 3-day course of chlormadinone acetate (2 mg/day) an...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)43213-9
更新日期：1978-04-01 00:00:00
abstract：:Women bear the predominant burden of our obesogenic environment, with a higher incidence of obesity than men, more impact on their fertility and success with treatment, and significant maternal and perinatal morbidity and mortality. In this series, the causes, consequences, and solutions regarding the obesity pandemic...
journal_title：Fertility and sterility
pub_type：
doi：10.1016/j.fertnstert.2017.02.110
更新日期：2017-04-01 00:00:00
abstract：:On the basis of studies in monkeys, the hypothesis has been proposed that vasectomy induces the formation of circulating immune complexes (CICs), which--via activation of the complement system--may result in immune injury of the endothelium and thereby exacerbate atherosclerosis; the same mechanism has been suggested ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1982-06-01 00:00:00
abstract：:Human menopausal gonadotropins (hMG) and clomiphene citrate (CC), either alone or in combination, are frequently used for in vitro fertilization (IVF) in an attempt to maximize the number of oocytes recovered and the number of embryos transferred. However, direct comparison of the relative efficacy of these protocols ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)49889-4
更新日期：1986-12-01 00:00:00
abstract：OBJECTIVE:To evaluate the effectiveness of sublingual misoprostol for cervical ripening before diagnostic hysteroscopy in premenopausal women. DESIGN:Placebo-controlled, double-blind, randomized trial. SETTING:University hospital. PATIENT(S):Fifty-two women with an indication for diagnostic hysteroscopy. INTERVENTI...
journal_title：Fertility and sterility
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.fertnstert.2009.01.073
更新日期：2010-05-01 00:00:00
abstract：OBJECTIVE:To compare pregnancy rates after fallopian tubal and uterine transfer of cryopreserved embryos. DESIGN:Prospective randomized trial with assignment to treatment groups by a random number table. SETTING:University of Iowa Hospitals and Clinics, a tertiary care academic institution. PATIENTS:Forty patients w...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0015-0282(16)57429-9
更新日期：1995-03-01 00:00:00
abstract：OBJECTIVE:To describe trends in age-specific incidence rates of female sterilization (FS) procedures in Western Australia and to evaluate the effects of the introduction of government-subsidized contraceptive methods and the implementation of the Australian government's baby bonus policy on FS rates. DESIGN:Population...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2016.04.035
更新日期：2016-09-01 00:00:00
abstract：:Carbohydrate and lipid metabolism was evaluated in 31 women before and after 1 year's use of a progesterone-releasing intrauterine device. The studies involved measuring blood glucose and plasma insulin levels during a 3-hour oral glucose tolerance test and also measuring fasting cholesterol and triglyceride levels. T...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)43932-4
更新日期：1979-04-01 00:00:00
abstract：:Defects of the DNA mismatch repair gene hMLH3 were screened by denaturing high-performance liquid chromatography and sequencing in germinal tissue DNA from patients with spermatogenic arrest, with sequence variations being confirmed in genomic DNA by polymerase chain reaction (PCR) direct sequencing analysis. Four mis...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2007.01.063
更新日期：2007-12-01 00:00:00
abstract：OBJECTIVE:To report a case of major intrauterine synechiae associated with ovarian function failure secondary to uterine devascularization. DESIGN:Case report. SETTING:Departments of gynecology and obstetrics in two hospitals in France. PATIENT(S):A 29-year-old woman, para 1, presenting severe postpartum hemorrhage....
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2004.07.975
更新日期：2005-03-01 00:00:00
abstract：OBJECTIVE:To investigate the relationship between sperm chromatin structure assay (SCSA) parameters, DNA fragmentation index (DFI) and high DNA stainability (HDS), and outcomes of IVF and intracytoplasmic sperm injection (ICSI). DESIGN:Retrospective review and prospective study. SETTING:Academic human reproduction la...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
doi：10.1016/j.fertnstert.2007.06.018
更新日期：2008-08-01 00:00:00
abstract：:Varicocelectomy has been found to improve the spermogram in many subfertile men. However, there still remains a sizable group of subfertile men whose counts fail to improve sufficiently following surgery. Clomiphene citrate, which has been shown to improve fertility in idiopathic oligospermia, was similarly tried in m...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)44661-3
更新日期：1980-04-01 00:00:00
abstract：OBJECTIVE:To review the use of radiotherapy for relieving the symptoms of recurrent endometriosis caused by functioning ovarian remnants. DESIGN:Retrospective study (case report). PATIENT(S):A woman with recurrent endometriosis of 14 years' duration. INTERVENTION(S):After hysterectomy and bilateral oophorectomy, hor...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(97)00342-7
更新日期：1997-11-01 00:00:00
abstract：:Two male cousins with partial 21-hydroxylase deficiency presented with bilateral testicular masses and infertility. In both cases, the testicular masses, consisting of adrenocorticotropic hormone-dependent pluripotential cells, were thought to play a major etiologic role in infertility. The administration of very smal...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)47485-6
更新日期：1983-12-01 00:00:00
abstract：OBJECTIVE:Report of a rare case of consecutive spontaneous cervical pregnancies. DESIGN:Case study. SETTING:Teaching hospital. PATIENT(S):One healthy nulliparous woman in the early years of her fourth decade. INTERVENTION(S):The first of two cervical pregnancies was treated with two doses of methotrexate and subseq...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2003.06.012
更新日期：2004-01-01 00:00:00
abstract：:This study indicates that SP occur in a higher percentage than theoretically expected after IVE-ET treatment, but it is not conclusive as to whether the SP is independent or a result of the treatment or operative laparoscopy. Our results are in agreement with those of previous studies that have shown that SP occur wit...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)49289-7
更新日期：1986-04-01 00:00:00
abstract：OBJECTIVE:To examine the results of 7 years of thawed ET during natural or controlled cycles using exogenous steroids. DESIGN:Retrospective evaluation to compare implantation and pregnancy rates with two protocols for transfer of cryopreserved-thawed pre-embryos. SETTING:Tertiary care academic center. PATIENTS:From ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)56943-x
更新日期：1994-09-01 00:00:00
abstract：OBJECTIVE:To evaluate the association between female cystic fibrosis (CF) carrier status and in vitro fertilization (IVF) response and outcomes. The presence of cystic fibrosis transmembrane conductance regulator (CFTR) gene mutations in male carriers has been associated with infertility, yet possible adverse effects o...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2014.07.1234
更新日期：2014-11-01 00:00:00
abstract：:There has been increasing interest in combining the oral agents clomiphene citrate (CC) and letrozole with gonadotropins in IVF: for poor responders to reduce the amount of gonadotropins used, and in normal responders to reduce the incidence of ovarian hyperstimulation (OHSS). In normal responders, mild stimulation wi...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
doi：10.1016/j.fertnstert.2017.08.017
更新日期：2017-10-01 00:00:00
abstract：:The possible association between employment in horticulture with potential exposure to pesticides and female infertility was examined by identification of women with hospital contact due to infertility and working in horticulture through the Danish Occupational Hospitalization Register. This follow-up study gave a sta...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2008.04.036
更新日期：2009-04-01 00:00:00
abstract：OBJECTIVE:To review rodent animal models of polycystic ovary syndrome (PCOS), with a focus on those associated with the metabolic syndrome and cardiovascular disease risk factors. DESIGN:Review. ANIMAL(S):Rodent models of PCOS. INTERVENTION(S):Description and comparison of animal models. MAIN OUTCOME MEASURE(S):Com...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
doi：10.1016/j.fertnstert.2012.04.006
更新日期：2012-07-01 00:00:00
abstract：OBJECTIVE:To study the distribution and progression of terminal hair growth in pregnant women and to determine the feasibility of a simplified scoring system for assessing hirsutism. DESIGN:Prospective follow-up observational study. SETTING:Academic hospital. PATIENT(S):A total of 115 pregnant women (discovery cohor...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2015.10.036
更新日期：2016-02-01 00:00:00
abstract：OBJECTIVE:To evaluate whether adolescent obesity is associated with difficulties in becoming pregnant later in life. DESIGN:Cross-sectional analysis of baseline data from a longitudinal cohort. SETTING:Multiethnic, community-based observational study of U.S. women. PATIENT(S):Three thousand one hundred fifty-four mi...
journal_title：Fertility and sterility
pub_type： 杂志文章,多中心研究
doi：10.1016/j.fertnstert.2008.12.059
更新日期：2010-04-01 00:00:00
abstract：OBJECTIVE:To assess whether IV tramadol before outpatient hysteroscopy could reduce procedure-related pain. DESIGN:A randomized double-blind placebo controlled trial. SETTING:Outpatient Hysteroscopy Centre in the Department of Obstetrics and Gynaecology of Cagliari University. PATIENT(S):Fifty healthy, parous, women...
journal_title：Fertility and sterility
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.fertnstert.2006.05.072
更新日期：2007-01-01 00:00:00
abstract：:Repetitively short luteal phases were found in eight infertile women. The short luteal phase was defined as 10 days or less from the presumed time of ovulation (as assessed by basal body temperature recording) to the onset of menses. Clomiphene citrate (Clomid) therapy resulted in pregnancy in two patients and lengthe...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章
doi：10.1016/s0015-0282(16)43930-0
更新日期：1979-04-01 00:00:00
