Abstract:
OBJECTIVE:To investigate the relationship between sperm chromatin structure assay (SCSA) parameters, DNA fragmentation index (DFI) and high DNA stainability (HDS), and outcomes of IVF and intracytoplasmic sperm injection (ICSI). DESIGN:Retrospective review and prospective study. SETTING:Academic human reproduction laboratory. PATIENT(S):Two hundred twenty-three couples undergoing conventional IVF (n = 137) and ICSI (n = 86). INTERVENTION(S):Testing with SCSA on a semen aliquot taken from ejaculate used for assisted reproductive technology (ART). MAIN OUTCOME MEASURE(S):Conventional semen parameters, DFI, HDS, outcomes of IVF and ICSI. RESULT(S):There were no significant differences in IVF and ICSI fertilization rate, good embryo rate, and pregnancy rate (PR) between high, moderate, and low DFI or HDS groups. Men with HDS >15% had significantly higher IVF abortion rates. There was a statistically insignificant trend toward an increased abortion rate in the high DFI (>27%) group. The DFI correlated negatively with sperm motility, and HDS correlated negatively with sperm morphology and concentration. CONCLUSION(S):Neither DFI nor HDS scores can provide independent information about embryo quality, fertilization, and PRs for infertility patients undergoing ART. Sperm DNA fragmentation probably affects sperm motility. The relationship between HDS and IVF abortion rates provides preliminary evidence that ICSI may be indicated for men with HDS >15%. The potential adverse effect of sperm DNA damage on the quality of postimplantation embryo and spontaneous abortion should be a concern.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Lin MH,Kuo-Kuang Lee R,Li SH,Lu CH,Sun FJ,Hwu YMdoi
10.1016/j.fertnstert.2007.06.018subject
Has Abstractpub_date
2008-08-01 00:00:00pages
352-9issue
2eissn
0015-0282issn
1556-5653pii
S0015-0282(07)01361-1journal_volume
90pub_type
杂志文章,评审abstract::Luteinization of an unruptured ovarian follicle has been reported as a cause of infertility and has heretofore been diagnosed only by direct visualization of the ovarian surface. Four patients who demonstrated defects in ovulation requiring therapy to induce ovulation and who were not achieving desired conception desp...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)46160-1
更新日期:1982-04-01 00:00:00
abstract::Semen analysis results obtained by a recently developed semi-automated Autosperm system (Amsaten N.V.S.A. Corp., De Pinte, Belgium) were compared with those obtained by the conventional, time-exposure photomicrographic, and automated CellSoft system (Cryo Resources Inc., New York, NY) analyses. The Autosperm system ei...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:
更新日期:1990-01-01 00:00:00
abstract:OBJECTIVE:To test for an association between DNA fragmentation and head shape at high magnification in fresh motile spermatozoa. DESIGN:Observational study. SETTING:Academic tertiary care center. PATIENT(S):A total of 60 men in our assisted reproductive program. INTERVENTION(S):Quantifying sperm head shape using el...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2013.01.100
更新日期:2013-05-01 00:00:00
abstract::The purpose of this article is to appraise the literature to ascertain whether the use of bariatric surgery should have a role in the contemporary management of the morbidly obese infertile patient before proceeding with infertility treatment. ...
journal_title:Fertility and sterility
pub_type: 杂志文章,meta分析,评审
doi:10.1016/j.fertnstert.2006.07.1499
更新日期:2007-02-01 00:00:00
abstract:OBJECTIVE:To investigate whether ghrelin signaling is involved in the pathogenesis of male factor infertility induced by leptin deficiency. DESIGN:Experimental study. SETTING:University academic medical center. ANIMAL(S):Ten-week-old C57BL/6J mice and ob/ob mice. INTERVENTION(S):Western blotting, (quantitative) rev...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2012.11.022
更新日期:2013-03-01 00:00:00
abstract::To evaluate the effect of luteal estrogen supplementation on pregnancy rates in normal responder IVF patients treated with recombinant FSH and fixed multidose GnRH antagonist, patients were randomized into two groups to receive vaginal 600 mg/d micronized progesterone for luteal phase supplementation with or without t...
journal_title:Fertility and sterility
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.fertnstert.2007.08.029
更新日期:2008-06-01 00:00:00
abstract:OBJECTIVE:To determine whether there is a difference in fibrinolytic compounds in endometriotic tissue, endometrium, peritoneal fluid (PF), and plasma from women with endometriosis and in endometrium and PF from healthy women. DESIGN:Prospective study. SETTING:Two university clinics. PATIENT(S):Regularly menstruatin...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(98)00285-4
更新日期:1998-11-01 00:00:00
abstract:OBJECTIVE:To observe the ultrastructure of human oocytes at different maturity stages and morphologies before and after in vitro maturation. DESIGN:Case report. SETTING:University-based center for human reproduction. PATIENT(S):A 28-year-old patient with polycystic ovary syndrome undergoing IVM/IVF-ET treatment. IN...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2009.02.010
更新日期:2009-07-01 00:00:00
abstract:OBJECTIVE:To provide a detailed summary of current scientific knowledge of somatic stem cells (SSCs) in murine and human myometrium and their putative implication in leiomyoma formation, as well as to establish new therapeutic options. DESIGN:Pubmed and Scholar One manuscripts were used to identify the most relevant s...
journal_title:Fertility and sterility
pub_type: 杂志文章,评审
doi:10.1016/j.fertnstert.2014.04.051
更新日期:2014-09-01 00:00:00
abstract:OBJECTIVE:To investigate whether quantitative analysis performed on one testicular specimen is adequate for quantitative evaluation of spermatogenic process. DESIGN:Comparison of quantitative analysis of spermatogenic cell types in testicular cytologic aspirates of various sites of each testis. SETTING:In each aspira...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)57741-3
更新日期:1995-08-01 00:00:00
abstract::A highly sensitive 2-hour tube test for the detection of human chorionic gonadotropin (hCG) in urine was compared with the more resource-demanding radioimmunoassay for hCG in serum as an aid in the diagnosis of ectopic pregnancy. In a first series of 107 patients with the possibility of ectopic pregnancy, the urine te...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)48559-6
更新日期:1985-05-01 00:00:00
abstract:OBJECTIVE:To study the effect of mifepristone on heparin-binding epidermal growth factor (HB-EGF) and its receptors HER1 and HER4 in the fallopian tube and in the endometrium. DESIGN:Prospective clinical study. SETTING:Hospital-based unit for obstetrics and gynecology and research laboratories. PATIENT(S):Healthy wo...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2005.08.011
更新日期:2006-01-01 00:00:00
abstract::On day 9 of an artificial menstrual cycle (28 day) in the rhesus monkey, endometrial epithelia and stroma and myometrial smooth muscle cells showed positive immunoreactivity for estradiol (E2) and progesterone (P) receptor. On day 23 a reduction of staining for E2 and P receptor was observed for epithelial cells in en...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)53531-6
更新日期:1990-05-01 00:00:00
abstract:OBJECTIVE:To survey the level of disclosure of conception method within families of children conceived by conventional in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) and to examine the factors that might influence parental attitudes and plans for disclosure. DESIGN:In-depth questionnaire. SET...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2004.12.019
更新日期:2005-04-01 00:00:00
abstract:OBJECTIVE:To evaluate prospectively the effects of high-dose, short-term treatment with a glucocorticoid in an attempt to normalize ovarian failure and induce pregnancy in women presenting with infertility. DESIGN:Uncontrolled, nonrandomized prospective study. SETTING:Two university-based reproductive endocrinology c...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)55915-9
更新日期:1993-05-01 00:00:00
abstract::Blood glucose and plasma insulin levels were measured during an oral glucose tolerance test in 18 women before and after 3 months' use of a low-estrogen oral contraceptive containing 0.035 mg of ethinylestradiol and 0.5 mg of norethindrone. There was no significant change in either of these two metabolic parameters. I...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)45620-7
更新日期:1981-07-01 00:00:00
abstract::Specialist training in reproductive medicine within Europe continues to evolve. Recent revisions, updates, and initiatives have helped to refine the core educational needs for the specialist trainee. ...
journal_title:Fertility and sterility
pub_type: 杂志文章,评审
doi:10.1016/j.fertnstert.2015.05.025
更新日期:2015-07-01 00:00:00
abstract::Evidence implicates the ovarian nerves in the regulation of hormonal and ovulatory functions. A technique for selectively denervating the in situ rabbit ovary is therefore of considerable investigative value. The ovarian artery was stripped of its adventitia and nerve bundles. Three weeks later, fluorescent histochemi...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:
更新日期:1975-04-01 00:00:00
abstract::In a prospective randomized fashion, this study evaluated embryo development in vitro after defragmentation versus assisted hatching alone of low grade day 3 embryos. Although a sustained decrease in day 5 fragmentation was observed in the defragmented group versus the assisted hatching only group, no difference in co...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2010.03.014
更新日期:2010-11-01 00:00:00
abstract::Based on American Society for Reproductive Medicine (ASRM) and Society for Assisted Reproductive Technology data available for 2010, ASRM's guidelines for the number of embryos to be transferred in in vitro fertilization cycles have been further refined in continuing efforts to reduce the number of higher-order multip...
journal_title:Fertility and sterility
pub_type: 杂志文章,实务指引
doi:10.1016/j.fertnstert.2012.09.038
更新日期:2013-01-01 00:00:00
abstract:OBJECTIVE:To study the association between two single-nucleotide polymorphisms (SNPs) variants (rs2241766 and rs1501299) of the adiponectin gene (ADIPOQ) and polycystic ovary syndrome (PCOS) in PCOS family trios. DESIGN:Family-based study. SETTING:University hospital. PATIENT(S):A total of 224 unrelated PCOS proband...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2014.01.035
更新日期:2014-05-01 00:00:00
abstract::Inflammatory exudates around intrauterine devices were found to produce large concentrations of prostaglandin F2alpha. To evaluate the effect of this substance, 100 semen specimens were incubated in concentrations 100 times greater than that found in normal semen. Motility indices were calculated, and significant inhi...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:
更新日期:1977-01-01 00:00:00
abstract::The present study details gross and histologic findings of 79 previously ligated fallopian tubes from 3 groups of patients. Of 20 oviducts removed after documented sterilization failure (group I), 6 revealed a process compatible with endometriosis. Four of nine previously ligated fallopian tubes removed at the Johns H...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)45251-9
更新日期:1981-01-01 00:00:00
abstract:OBJECTIVE:To determine if pentoxifylline has an in vitro effect on human sperm motility in subjects with normal semen analysis. DESIGN:Prospective, longitudinal study of the effect of pentoxifylline on normospermic semen samples using computerized analysis. SETTING:The Assisted Conception Unit, Royal Maternity Hospit...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)55708-2
更新日期:1993-02-01 00:00:00
abstract:OBJECTIVE:To report a case of a woman who presented with parasitic myomas 27 months after laparoscopic myomectomy. DESIGN:Case report. SETTING:University medical center. PATIENT(S):A 31-year-old G0 woman who had a previous history significant for laparoscopic myomectomy with morcellation 27 months before. INTERVENT...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2008.08.014
更新日期:2009-03-01 00:00:00
abstract:OBJECTIVE:To determine the probability of detecting the gestational sac of a normal intrauterine pregnancy by vaginal ultrasound at different gestational ages and serum hCG concentrations. DESIGN:Prospective, randomized study. SETTING:Pregnant human volunteers in a university-based clinical research environment. PAT...
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/s0015-0282(16)56749-1
更新日期:1994-06-01 00:00:00
abstract:OBJECTIVE:To describe two cases of in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) with testicular sperm in men with immotile cilia syndromes. DESIGN:Case report. SETTING:A university-based male infertility clinic and assisted reproduction unit. PATIENT(S):Two couples with male factor infert...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(01)01974-4
更新日期:2001-09-01 00:00:00
abstract::The effect of danazol on steroidogenesis in cultured human granulosa cells was studied. Granulosa cells obtained from antral follicles in the mid to late follicular phase were cultured for 2 to 6 days. Danazol (0.1 to 5 micrograms/ml) and human chorionic gonadotropin (hCG, 10 IU/ml), alone or in combination, were adde...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:
更新日期:1986-08-01 00:00:00
abstract::The immunobead test was used to study the immunoglobulin class of antibodies on sperm before and after penetration through a microcolumn of cervical mucus. Sixteen men with positive sperm antibodies (positive sperm immobilization test) and sperm that penetrated cervical mucus in prior tests were selected for study. Ho...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)48916-8
更新日期:1985-10-01 00:00:00
abstract:OBJECTIVE:To determine the prognostic impact of the nuclear status at the two-cell stage on intracytoplasmic sperm injection (ICSI) outcomes. DESIGN:Retrospective study. SETTING:Hospital. PATIENT(S):Only ICSI cycles with time-lapse monitoring of transferred embryos with known implantation/delivery data from November...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2016.09.022
更新日期:2017-01-01 00:00:00