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Embryo multinucleation at the two-cell stage is an independent predictor of intracytoplasmic sperm injection outcomes.

Abstract：

OBJECTIVE:To determine the prognostic impact of the nuclear status at the two-cell stage on intracytoplasmic sperm injection (ICSI) outcomes. DESIGN:Retrospective study. SETTING:Hospital. PATIENT(S):Only ICSI cycles with time-lapse monitoring of transferred embryos with known implantation/delivery data from November 2012 to December 2014 were included. A total of 2,449 embryos were assessed for multinucleation rates at the two- and four-cell stage, and 608 transferred embryos were studied for ICSI outcomes. INTERVENTION(S):None. MAIN OUTCOME MEASURE(S):Implantation rate (IR) and live birth rate (LBR) according to the number of multinucleated blastomeres at the two-cell stage: none (Without-MNB2cell), one (MNB1/2cell), and two (MNB2/2cell); morphokinetics of MNB2cell embryos. RESULT(S):Embryos with MNB1/2cell led to lower IR (27.7%) and LBR (22.7%) than embryos Without-MNB2cell (33.4% and 29.8%, respectively). The MNB2/2cell embryos led to significantly lower IR (18.3%) and LBR (13.4%) than embryos Without-MNB2cell. This difference remained significant in multivariate analysis for implantation (odds ratio 0.57; 95% confidence interval 0.34-0.94) and birth (odds ratio 0.46; 95% confidence interval 0.26-0.80), independently of the other significant parameters (women's age, time of two-cell formation, and multinucleation at the four-cell stage). Among implanted MNB2cell, if cleavage into four cells occurred later than 37 hours after insemination, embryos were significantly more likely to lead to birth. CONCLUSION(S):The presence of multinucleation at the two-cell stage and more specifically in both blastomeres had a significant negative impact on birth potential. Thus, embryo multinucleation at the two-cell stage should be used as an additional noninvasive criterion for embryo selection.

journal_name

Fertil Steril

journal_title

Fertility and sterility

authors

Desch L,Bruno C,Luu M,Barberet J,Choux C,Lamotte M,Schmutz E,Sagot P,Fauque P

doi

10.1016/j.fertnstert.2016.09.022

subject

Has Abstract

pub_date

2017-01-01 00:00:00

pages

97-103.e4

issue

eissn

0015-0282

issn

1556-5653

pii

S0015-0282(16)62842-X

journal_volume

107

pub_type

杂志文章

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Embryo multinucleation at the two-cell stage is an independent predictor of intracytoplasmic sperm injection outcomes.