Abstract：
OBJECTIVE:To evaluate sperm DNA fragmentation in normozoospermic male partners of couples undergoing infertility evaluation. DESIGN:Retrospective cohort study. SETTING:Clinical andrology laboratory. PATIENT(S):A total of 1,974 consecutive normozoospermic men selected from a larger cohort of 4,345 consecutive, nonazoospermic men presenting for infertility evaluation. INTERVENTION(S):None. MAIN OUTCOME MEASURE(S):Clinical parameters, conventional semen parameters, and sperm DNA fragmentation assessed by flow cytometry-based TUNEL assay and reported as percent sperm DNA fragmentation (%SDF). RESULT(S):The mean (± SD) %SDF and the proportion of men with high %SDF (>30%) were significantly lower in the normozoospermic compared with the entire cohort of 4,345 evaluable infertile men (17.6% ± 10.1% vs. 20.7% ± 12.4% and 11% vs. 20%, respectively). In the group of 1,974 normozoospermic men, %SDF was positively correlated with paternal age (r = 0.17) and inversely correlated with progressive motility (r = -0.26). In the subset of normozoospermic men with sperm parameters above the 50th percentile (≥ 73 × 10(6) sperm/mL, ≥ 55% progressive motility, and ≥ 14% normal forms, World Health Organization 2010 guidelines), 5% (4 of 83) had elevated %SDF (>30%). CONCLUSION(S):In this large cohort of normozoospermic men presenting for infertility evaluation, DNA fragmentation level is related to sperm motility and paternal age, and 11% of these men have high levels of sperm DNA fragmentation. Furthermore, the data indicate that a nonnegligible proportion (5%) of normozoospermic men with high-normal sperm parameters may also have significant sperm DNA fragmentation.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Belloc S,Benkhalifa M,Cohen-Bacrie M,Dalleac A,Amar E,Zini Adoi
10.1016/j.fertnstert.2014.02.006subject
Has Abstractpub_date
2014-06-01 00:00:00pages
1588-93issue
6eissn
0015-0282issn
1556-5653pii
S0015-0282(14)00144-7journal_volume
101pub_type
杂志文章abstract：:Nine anovulatory patients with hypothalamic-pituitary dysfunction were treated with d-Trp6-luteinizing hormone-releasing hormone, an analog with far greater gonadotropin-releasing activity than luteinizing hormone-releasing hormone. Four of eight patients, who were formerly unsuccessfully treated with clomiphene, huma...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章
doi：
更新日期：1978-04-01 00:00:00
abstract：OBJECTIVE:To examine the effects of different thermodynamic control systems on the temperature stability of human eggs during in vitro manipulation, with the integrity of meiotic spindles imaged using the LC-PolScope (Cambridge Research & Instrumentation, Inc., Woburn, MA). DESIGN:We performed intracytoplasmic sperm i...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(02)03117-5
更新日期：2002-06-01 00:00:00
abstract：OBJECTIVE:To understand the barriers that serodiscordant couples with human immunodeficiency virus (HIV) face in accessing services for risk reduction and infertility using assisted reproductive technology (ART). DESIGN:Two-arm cross-sectional telephone "secret shopper" study. SETTING:Infertility clinics designated b...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2017.11.039
更新日期：2018-03-01 00:00:00
abstract：:The steroidogenic activity of purified theca interstitial cells (TIC) from polycystic ovaries of a patient with virilizing congenital adrenal hyperplasia due to 21-hydroxylase deficiency was studied in serum-free medium. The results show that CVAH TIC have high intrinsic basal androgen secretory activity that is drama...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)60450-8
更新日期：1989-01-01 00:00:00
abstract：:The amino acid and protein composition of human follicular fluid, obtained during surgery from women with polycystic ovaries, and of a simultaneously obtained sample of blood plasma were studied. In general, amino acid concentrations were higher in follicular fluid than in blood plasma: only the concentration of Cys w...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)42324-1
更新日期：1977-01-01 00:00:00
abstract：OBJECTIVE:To extend and confirm earlier observations that serial CA-125 determinations appear to have prognostic value in some infertile women with surgically treated endometriosis using a new automated enzyme immunoassay. DESIGN:A prospective consecutive case series. SETTING:A university-based tertiary practice. PA...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1995-08-01 00:00:00
abstract：OBJECTIVE:To determine the effect of sonographer experience during ultrasound guided embryo transfer on pregnancy outcomes. DESIGN:Retrospective chart review. SETTING:A university-based academic practice. PATIENT(S):A total of 319 women who underwent in vitro fertilization and embryo transfer (IVF-ET) from the site-...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2008.07.1743
更新日期：2009-09-01 00:00:00
abstract：:Two successive intranasal doses (300 micrograms at 8:00 A.M. and 6:00 P.M.) of the luteinizing hormone-releasing hormone (LH-RH) agonist (D-Ser[TBU]6-des-Gly-NH2(10))LH-RH ethylamide (Buserelin) were administered on days 1 to 10 after the midcycle luteinizing hormone (LH) surge in 33 normally cycling women. Maximal st...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)47062-7
更新日期：1983-05-01 00:00:00
abstract：OBJECTIVE:To investigate the impact of intracytoplasmic sperm injection (ICSI) and assisted hatching (AH) on assisted reproductive technology (ART) outcomes in initial cycles with diminished ovarian reserve (DOR) as the primary diagnosis. DESIGN:Retrospective cohort study of cycles from the Society for Assisted Reprod...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2014.06.043
更新日期：2014-10-01 00:00:00
abstract：OBJECTIVE:To identify the cumulus cell gene expression associated with oocyte developmental competence, specifically live birth, after single ET (SET) assisted reproductive technology. DESIGN:Retrospective gene expression analysis in human cumulus cells from oocytes that established a pregnancy resulting in live birth...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2011.04.033
更新日期：2011-07-01 00:00:00
abstract：OBJECTIVE:To investigate, with pre-COVID-19 data, whether parental exposure to severe systemic infections near the time of conception is associated with pregnancy outcomes. DESIGN:Retrospective cohort study. SETTING:Population-based study covering births within the United States from 2009 to 2016. PARTICIPANTS:The I...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2020.09.153
更新日期：2020-12-01 00:00:00
abstract：OBJECTIVE:To assess the association of total isoflavone intake with ovulatory function, including sporadic anovulation in healthy premenopausal women. DESIGN:Prospective cohort study. SETTING:University. PATIENT(S):Participants included 259 healthy regularly menstruating women aged 18-44 years. INTERVENTION(S):None...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2013.08.002
更新日期：2013-12-01 00:00:00
abstract：OBJECTIVE:To investigate the expression kinetics of interleukin-1 receptors (IL-1R), receptor antagonist (IL-1RN), and monocyte chemotactic protein 1 (MCP-1) throughout early gestation in mice. DESIGN:Assessment of IL-1R, IL-1RN, and MCP-1 throughout early pregnancy. SETTING:Reproduction laboratory. ANIMAL(S):B6C3F1...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2014.01.011
更新日期：2014-04-01 00:00:00
abstract：OBJECTIVE:To present the success rates of assisted reproductive technologies (ART) cycles performed in 2001 in Canada. DESIGN:Retrospective cohort study. SETTING:Nineteen of 22 ART centers in Canada. PARTICIPANTS:Couples undergoing ART treatment in Canada during 2001. METHODS:Data on each ART cycle performed during...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2005.03.037
更新日期：2005-09-01 00:00:00
abstract：OBJECTIVE:To review the discovery of chromothripsis and analyze its impact on human reproduction. DESIGN:Database and literature analysis. SETTING:University hospital. PATIENT(S):Carriers of massive and complex chromosomal rearrangements. INTERVENTION(S):Cytogenetic analysis and molecular testing (fluorescence in s...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
doi：10.1016/j.fertnstert.2014.09.006
更新日期：2014-12-01 00:00:00
abstract：OBJECTIVE:To obtain proof-of-concept that locally produced anti-inflammatory steroids suppress ovulation-associated extracellular matrix proteases in human ovarian surface epithelial (OSE) cells. DESIGN:Primary OSE cell cultures treated with interleukin-1alpha (IL-1alpha) (500 pg/mL) as proxy for inflammation, with/wi...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2008.06.040
更新日期：2009-08-01 00:00:00
abstract：OBJECTIVE:To determine if the decline in infertility has been uniform across subgroups. DESIGN:Periodic data from the National Fertility Survey and the National Survey of Family Growth were used to determine which factors contributed to the decline in 12-month infertility in the United States. SETTING:National Survey...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2006.02.129
更新日期：2006-09-01 00:00:00
abstract：:Standardized, high-quality training in reproductive endocrinology, infertility, and assisted reproductive technologies (REI-ART) faces challenges owing to the high-tech nature of ART and the important country-to-country differences in clinical practice and regulations overseeing training. Moreover, while the training ...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
doi：10.1016/j.fertnstert.2015.04.031
更新日期：2015-07-01 00:00:00
abstract：:Adhesion formation after laparoscopic surgery was evaluated in mice of different strains. More adhesions were observed in Swiss, NMRI, and BALB/c mice, with less interanimal variability in BALB/c mice. These data point to genetics effects on adhesion formation, which open new insights in its pathogenesis and indicate ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2004.11.084
更新日期：2005-06-01 00:00:00
abstract：OBJECTIVE:To assess the relative efficacy, in terms of clinical pregnancy rates (PRs), of transuterotubal insemination versus the more traditional intrauterine insemination (IUI) procedure. DESIGN:Prospective, randomized, cross-over. SETTING:University-affiliated tertiary care center. PATIENTS:One hundred sixty infe...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章,随机对照试验
doi：
更新日期：1992-07-01 00:00:00
abstract：OBJECTIVE:Evaluate racial differences in reproducibility of hormone levels over time (estradiol, DHEAS, FSH, and testosterone) while adjusting for covariates previously identified as relevant in the study population. DESIGN:Longitudinal cohort study. SETTING:Healthy, late-reproductive-age women in a community-based s...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(00)01723-4
更新日期：2001-02-01 00:00:00
abstract：OBJECTIVE:To compare the FSH threshold level for follicle growth up to the preovulatory stage between superovulated and natural cycles. DESIGN:Normally cycling women were investigated during an untreated spontaneous cycle (control) and three cycles treated with FSH. SETTING:Infertility clinic in a university hospital...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)57610-9
更新日期：1995-06-01 00:00:00
abstract：:In this observational, case-control study in infertile women, we observed elevated serum oxidative stress that was significantly correlated with an increase in serum paraoxonase-1 activity and without any evidence of a proinflammatory reaction. These results suggest a protective role of paraoxonase-1 against inflammat...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2009.11.043
更新日期：2010-08-01 00:00:00
abstract：:This immunohistochemical study shows that platelet endothelial cell adhesion molecule-1 (PECAM-1), known to play a role in neoangiogenesis and vascular development, is strongly and homogeneously expressed in endothelial cells lining blood vessels from red and black pelvic endometriotic lesions. The distribution of PEC...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2004.05.075
更新日期：2004-10-01 00:00:00
abstract：OBJECTIVE:To develop a reliable, reproducible, and sensitive method for investigating gene-expression profiles from individual human oocytes. DESIGN:Five commercially available protocols were investigated for their efficiency to amplify messenger RNA (mRNA) from 54 single human oocytes. Protocols resulting in sufficie...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2015.10.025
更新日期：2016-02-01 00:00:00
abstract：:High-volume intravenous urography was performed in 63 women with surgically proven endometriosis. Subtle abnormalities were found in 15.9% of these women. No patient had urologic symptoms, and there was no evidence of hydroureter or ureteral obstruction on the IVP. Long-term follow-up study will be required to determi...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)49900-0
更新日期：1986-12-01 00:00:00
abstract：OBJECTIVE:To evaluate the levels of interleukin-1beta (IL1beta) and its inhibitory soluble interleukin-1 receptor type II (IL1R2) in the peritoneal fluid (PF) of normal women and patients with endometriosis suffering from pelvic pain and infertility. DESIGN:Retrospective study using ELISA to measure peritoneal fluid I...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2007.06.019
更新日期：2008-06-01 00:00:00
abstract：OBJECTIVE:To examine the relationship between an antibody against GAPDH-2, a sperm-specific protein, and infertility of female mice. DESIGN:Basic research. SETTING:National Research Institute for Family Planning Beijing, World Health Organization Collaboration Center of Human Reproduction. ANIMAL(S):New Zealand rabb...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2008.09.001
更新日期：2009-12-01 00:00:00
abstract：:There is increasing evidence that a behavioral treatment approach might be efficacious in the treatment of the emotional aspects of infertility and may lead to increased conception rates. The first 54 women to complete a behavioral treatment program based on the elicitation of the relaxation response showed statistica...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)53275-0
更新日期：1990-02-01 00:00:00
abstract：OBJECTIVE:To develop a reliable sperm test that would predict pregnancy rate in assisted reproductive technologies. DESIGN:Blind prospective cohort study. SETTING:Tertiary-care, university hospital-affiliated IVF program. PATIENTS:One hundred nineteen sperm samples were obtained from 110 males from couples undergoin...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章
doi：10.1016/s0015-0282(16)58107-2
更新日期：1996-02-01 00:00:00
