Abstract：
OBJECTIVE:To standardize and validate an assay for reactive oxygen species (ROS) in human semen. DESIGN:ROS levels assayed in blanks, negative and positive control samples (30% H2O2), and human semen, with the use of a luminol-based chemiluminescence assay measured in a single tube luminometer. SETTING:Andrology laboratory. PATIENT(S):Semen samples from 19 men attending for routine semen analysis. INTERVENTION(S):None. MAIN OUTCOME MEASURE(S):ROS levels reported in relative light units (RLU) per second, adjusted for sperm concentration. RESULT(S):The ROS assay equipment performed according to expectations, generating a chemiluminescence signal for positive control samples and semen samples that decayed rapidly and was captured within 10 minutes. Blanks and negative control samples gave negligible readings. There was no significant intra- or interassay variation. Interference from extraneous factors was negligible. The assay distinguished changes in ROS over a wide range of concentrations and provided consistent results between reagent batches. Working reagents remained stable for 3 months. Acceptable levels for negative and positive control samples were established to set criteria for the test passing or failing on any given day. The assay was sensitive to ambient temperature >25°C. ROS declined significantly with time after ejaculation. Mechanical agitation doubled ROS production in semen. CONCLUSION(S):These results validate the ROS assay and demonstrate that it is a highly reliable and accurate diagnostic test.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Vessey W,Perez-Miranda A,Macfarquhar R,Agarwal A,Homa Sdoi
10.1016/j.fertnstert.2014.09.009subject
Has Abstractpub_date
2014-12-01 00:00:00pages
1576-83.e4issue
6eissn
0015-0282issn
1556-5653pii
S0015-0282(14)02161-Xjournal_volume
102pub_type
杂志文章abstract：OBJECTIVE:To evaluate the results of IVF in women > or = 40 years of age using their own oocytes. DESIGN:Retrospective study. SETTING:Wolfson and Royal Masonic in vitro fertilization units, London, United Kingdom. PATIENT(S):A total of 1,087 IVF cycles were started in women > or = 40 years of age. INTERVENTION(S):M...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(98)00353-7
更新日期：1998-12-01 00:00:00
abstract：OBJECTIVE:To evaluate the effects of resveratrol on growth and function of granulosa cells. Previously, we demonstrated that resveratrol exerts profound proapoptotic effects on theca-interstitial cells. DESIGN:In vitro study. SETTING:Research laboratory. ANIMAL(S):Immature Sprague-Dawley female rats. INTERVENTION(S...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2012.08.004
更新日期：2012-12-01 00:00:00
abstract：:The presence of progestogens in the cervical mucus suppresses and arrests sperm penetration. Using the Kremer technique, the effects of in vitro released progesterone, d-norgestrel, and cyproterone acetate were studied by inserting silicone rubber threads bearing the respective compounds into capillary tubes containin...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)40875-7
更新日期：1975-01-01 00:00:00
abstract：:Standardized, high-quality training in reproductive endocrinology, infertility, and assisted reproductive technologies (REI-ART) faces challenges owing to the high-tech nature of ART and the important country-to-country differences in clinical practice and regulations overseeing training. Moreover, while the training ...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
doi：10.1016/j.fertnstert.2015.04.031
更新日期：2015-07-01 00:00:00
abstract：OBJECTIVE:To investigate the role of heart and neural crest derivatives-expressed transcript 2 (HAND2) during decidualization of human endometrial stromal cells (ESCs). DESIGN:In vitro experiment. SETTING:Research laboratory. PATIENT(S):Twenty-six patients undergoing hysterectomy for benign reasons. INTERVENTION(S)...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2014.03.013
更新日期：2014-06-01 00:00:00
abstract：OBJECTIVE:To assess the effect of age and ovarian function on endometrial receptivity. DESIGN:Retrospective comparison between standard IVF and ovum donation in younger and older patients (< 40 and > or = 40 years of age, respectively). PATIENTS:In standard IVF, there were 325 transfer cycles in older patients and 1,...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)56104-4
更新日期：1993-08-01 00:00:00
abstract：OBJECTIVE:To reduce estrogen exposure in women with endometrial cancer undergoing in vitro fertilization using an aromatase inhibitor. DESIGN:Prospective case series. SETTING:Academic center for reproductive medicine. PATIENT(S):Endometrial carcinoma patients presenting for fertility preservation or fresh embryo tra...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2006.12.068
更新日期：2007-09-01 00:00:00
abstract：OBJECTIVE:To evaluate clinical outcomes of day 2 versus day 3 ET using a culture media with no glucose or phosphate. DESIGN:Retrospective clinical study. SETTING:Hospital-based fertility clinic. PATIENT(S):One hundred seventy-six IVF-ET patients undergoing controlled ovarian supraovulation. INTERVENTION(S):IVF and ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(97)00499-8
更新日期：1998-02-01 00:00:00
abstract：:Appropriate endometrial maturation is of paramount importance to achieve reproductive success. Practical and ethical considerations require that in vitro methods be available to evaluate regulation of human endometrial function. Additionally, tissue complexity requires separation of individual cell populations. This r...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)53160-4
更新日期：1989-12-01 00:00:00
abstract：:Tissue microarrays (TMAs) are useful tools for studying protein expression in endometriosis. Tissue microarray analyses of immunohistochemical profiles of estrogen receptor-alpha and P receptor corroborate previously published results from the use of conventional immunohistochemistry, thus validating TMA use in endome...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2004.06.044
更新日期：2004-12-01 00:00:00
abstract：OBJECTIVE:To measure free T, free E2, and sex hormone-binding globulin (SHBG) levels in oligozoospermic men with varicoceles. DESIGN:Retrospective study. PATIENTS:Sixty-four infertile patients with varicoceles. SETTING:A university-based tertiary care referral center. INTERVENTIONS:Assessment of seminal cellular ch...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1996-08-01 00:00:00
abstract：:Fifteen subfertile males with absent or atrophic right testes underwent ligation of left varicoceles. Sperm concentration rose significantly with correction of scrotal thermographic abnormality and nine wives (60%) became pregnant. ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)55098-5
更新日期：1992-06-01 00:00:00
abstract：OBJECTIVE:To investigate whether zona drilling of oocytes, a technique used to locally disrupt the zona pellucida, causes important changes in intracellular pH. DESIGN AND METHODS:In a first set of experiments, intracellular pH was measured during zona drilling of mouse oocytes, using pH-sensitive microelectrodes. In ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)56525-x
更新日期：1994-02-01 00:00:00
abstract：:The present study has demonstrated the usefulness of GH in augmenting buserelin acetate/hMG to stimulate the rate of growth of follicles in women regarded as poor responders. The PR achieved was extremely encouraging in a group of patients whose prognosis was otherwise poor. Further studies are required to confirm the...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章
doi：10.1016/s0015-0282(16)54084-9
更新日期：1991-01-01 00:00:00
abstract：:Building families through the adoption of children has been supported by human society throughout history. The ethical appropriateness of patients donating embryos to other patients for family building, or for research, is well established and is affirmed by this Committee. The use of the term ''adoption'' for embryos...
journal_title：Fertility and sterility
pub_type： 杂志文章,实务指引
doi：10.1016/j.fertnstert.2016.03.017
更新日期：2016-07-01 00:00:00
abstract：OBJECTIVE:To evaluate the impact of female smoking on early embryo development in vitro using a time-lapse system. DESIGN:Retrospective analysis of prospectively collected database. SETTING:Assisted reproductive technology (ART) program. PATIENT(S):A total of 135 infertile couples undergoing IVF-intracytoplasmic spe...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2013.01.136
更新日期：2013-06-01 00:00:00
abstract：:Pregnancy outcomes and adverse outcomes in infertility trials are reported to varying extents; for example, 35% of clinical trials reported no information on pregnancy loss, only 43% reported adverse events during the preconception treatment period, and only 7% reported any serious adverse events. Incomplete reporting...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
doi：10.1016/j.fertnstert.2011.02.040
更新日期：2011-06-30 00:00:00
abstract：OBJECTIVE:To determine whether follicular fluid (FF) cortisol levels affect cumulus cell (CC) lipid content during oocyte meiotic resumption, and whether CCs express genes for glucocorticoid action. DESIGN:Prospective cohort study. SETTING:Academic medical center. PATIENT(S):Thirty-seven nonobese women underwent ova...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章
doi：10.1016/j.fertnstert.2014.09.034
更新日期：2015-01-01 00:00:00
abstract：OBJECTIVE:The present study examines the safety, effectiveness, and local tissue response for a new transcervical fallopian tube permanent contraceptive device, the STOP device (Conceptus, Inc., San Carlos, CA). DESIGN:Nonrandomized prospective evaluation of tubal occlusion and histologic response. SETTING:Inpatient,...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(01)02858-8
更新日期：2001-11-01 00:00:00
abstract：:Eight premenopausal women with cancer had blood drawn for analysis of brain-derived neurotrophic factor (BDNF) and müllerian-inhibiting substance (MIS) before and 3 months after receiving chemotherapy. Unlike MIS, BDNF levels were not reduced after chemotherapy. ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2010.10.033
更新日期：2011-04-01 00:00:00
abstract：:With advances in cancer therapies, survival rates in prepubescent patients have steadily increased. However, a number of these surviving patients have been rendered sterile owing to their rigorous oncologic treatment regimens. In addition to cancer treatments, men and women, who are genetically fertile, can become inf...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
doi：10.1016/j.fertnstert.2013.11.025
更新日期：2014-01-01 00:00:00
abstract：OBJECTIVE:To investigate the ability of N-formyl-methionyl-leucyl-phenylalanine (f-MLP), complement 5a (C5a), and nerve growth factor (NGF) to stimulate human spermatozoal reactive oxygen species generation in fertile and infertile patients. DESIGN:Prospective, controlled study measuring human spermatozoal reactive ox...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)55874-9
更新日期：1993-04-01 00:00:00
abstract：:This study examined coping with infertility and how coping is related to depression for men and women. Results showed that both men and women who engage in a disproportionate degree of escape/avoidance behavior and acceptance of responsibility for infertility were more vulnerable to symptoms of depression. ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2005.09.020
更新日期：2006-03-01 00:00:00
abstract：OBJECTIVE:To investigate adrenal steroid regulation in polycystic ovary syndrome. DESIGN:Five-hour oral glucose tolerance test (OGTT) and frequently sampled-intravenous gluclose tolerance test. SETTING:University research center. PATIENT(S):Thirty patients. INTERVENTION(S):None. MAIN OUTCOME MEASURE(S):Anthropomet...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2009.03.017
更新日期：2010-03-01 00:00:00
abstract：OBJECTIVE:To investigate whether interleukin-1beta (IL-1beta) and interleukin-1alpha (IL-1alpha) affect the implantation rate of patients undergoing IVF-ET. DESIGN:Follicular fluid and serum were obtained on the day of hCG administration, the day of oocyte retrieval, and the day of embryo transfer. SETTING:Cellular i...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章
doi：10.1016/s0015-0282(98)00243-x
更新日期：1998-09-01 00:00:00
abstract：OBJECTIVES:To assess the impact of a levonorgestrel-releasing implant contraceptive (Norplant; Wyeth-Ayerst Laboratories, Philadelphia, PA) on glucose metabolism. DESIGN:Prospective evaluation of insulin action and secretion in women under hyperglycemic hyperinsulinemic clamp conditions in the midfollicular phase befo...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章
doi：
更新日期：1995-04-01 00:00:00
abstract：:We conducted a case-control study and compared the results of IVF treatment in two groups of women with polycystic ovaries. The first group consisted of 42 women who were previously treated unsuccessfully with in vitro maturation (IVM), and the second group, 48 others with no previous IVM (control group). We included ...
journal_title：Fertility and sterility
pub_type： 信件
doi：10.1016/j.fertnstert.2009.09.033
更新日期：2010-06-01 00:00:00
abstract：:A prospective crossover study comparing ovulation induction techniques in eight women with premature ovarian failure is presented. These patients were treated with FSH rebound techniques using the GnRH-a, LA, alone in one treatment cycle and the same plus menopausal gonadotropins in the other treatment cycle. Two wome...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)54863-8
更新日期：1992-02-01 00:00:00
abstract：:Seven nonionic detergents of the Igepal CO series differing in molecular size and including nonoxynol-9 and a representative anionic (SDS) and cationic (Cepacol) detergent were tested as to their relative cytotoxicity. The biologic effects of these detergents on human W1--38 fibroblasts (DNA and glycosaminoglycans syn...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1980-05-01 00:00:00
abstract：:No nerve fibers are found in endometriomas or in the endometrioma-containing ovaries. These findings are consistent with our previous report that no correlation exists between the presence of nerve fibers and endometriosis. ...
journal_title：Fertility and sterility
pub_type： 信件
doi：10.1016/j.fertnstert.2003.11.049
更新日期：2004-07-01 00:00:00
