Abstract：
OBJECTIVE:To investigate the ability of N-formyl-methionyl-leucyl-phenylalanine (f-MLP), complement 5a (C5a), and nerve growth factor (NGF) to stimulate human spermatozoal reactive oxygen species generation in fertile and infertile patients. DESIGN:Prospective, controlled study measuring human spermatozoal reactive oxygen species generation after addition of f-MLP, C5a, or NGF. SETTING:A large health maintenance organization. PATIENTS, PARTICIPANTS:The fertile group consisted of 14 men with established fertility and normal bulk semen parameters. The infertile group was comprised of 8 men who were infertile after > 18 months of unprotected sexual intercourse. INTERVENTIONS:The sperm samples were subjected to four test conditions: f-MLP stimulation, C5a stimulation, NGF stimulation, and no stimulation (control). MAIN OUTCOME MEASURE:Reactive oxygen generation was measured over a 15-minute period using the method of chemiluminescence. RESULTS:In both the fertile and infertile groups, reactive oxygen species generation was significantly enhanced by f-MLP, C5a, and NGF compared with controls. No significant difference in f-MLP- and C5a-stimulated reactive oxygen production was demonstrated between the infertile and fertile groups; however, there was a significant difference in reactive oxygen generation between infertile and fertile subjects when stimulated with NGF. CONCLUSIONS:The current study represents the first report of f-MLP-, C5a-, and NGF-stimulated reactive oxygen species generation by human spermatozoa. Nerve growth factor enhanced reactive oxygen species production to a greater extent in infertile subjects compared with fertile subjects. This points to a possible NGF-mediated biochemical defect in the sperm of infertile patients.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Weese DL,Peaster ML,Hernandez RD,Leach GE,Lad PM,Zimmern PEdoi
10.1016/s0015-0282(16)55874-9subject
Has Abstractpub_date
1993-04-01 00:00:00pages
869-75issue
4eissn
0015-0282issn
1556-5653pii
S0015-0282(16)55874-9journal_volume
59pub_type
杂志文章abstract：OBJECTIVE:To investigate the effect of vitrification and beta-mercaptoethanol (beta-ME) on reactive oxygen species (ROS) activity and in vitro development of oocytes vitrified before or after in vitro fertilization (IVF). DESIGN:Randomized prospective study. SETTING:University-based assisted reproductive technology l...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2010.01.043
更新日期：2010-05-15 00:00:00
abstract：:The hyperandrogenic-insulin-resistant acanthosis nigricans syndrome affects between 2% and 5% of hirsute women and is characterized by INS resistance, elevated INS levels, acanthosis nigricans, and androgen excess. These patients' response to therapy is unclear, although long-acting GnRH-a suppression has been propose...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1994-03-01 00:00:00
abstract：OBJECTIVE:To provide an up-to-date review of studies that have examined the relative role of endometrial development in in vitro fertilization (IVF) and embryo transfer (ET) treatment in relation to the treatment outcome. DATA IDENTIFICATION:The most important published studies and personal communications related to t...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
doi：10.1016/s0015-0282(16)55008-0
更新日期：1992-05-01 00:00:00
abstract：OBJECTIVE:To review and evaluate published studies that have assessed the effect on the skeleton of treatment with gonadotropin-releasing hormone agonists (GnRH-a). The effects on bone biochemistry and bone density are presented. DATA IDENTIFICATION:Major studies reporting on bone and GnRH-a use were identified throug...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
doi：10.1016/s0015-0282(16)54948-6
更新日期：1992-04-01 00:00:00
abstract：OBJECTIVE:To compare the effectiveness and safety of the Filshie Clip and Tubal Ring systems when applied via minilaparotomy and laparoscopy. DESIGN:Prospective, multicenter randomized controlled clinical trial, with postoperative evaluation by a physician who was masked to the operative technique. SETTING:Healthy vo...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章,多中心研究,随机对照试验
doi：10.1016/s0015-0282(00)00696-8
更新日期：2000-09-01 00:00:00
abstract：:Hemochromatosis is a rare disorder of iron storage. This report illustrates a case of hypogonadotropic-hypogonadism in a female with biopsy-proven hemochromatosis. Dynamic pituitary and gonadal testing revealed subnormal gonadotropin responses to gonadotropin-releasing hormone (GnRH) but normal ovarian reserve, as sho...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)53842-4
更新日期：1990-10-01 00:00:00
abstract：OBJECTIVE:To determine the genetic cause of primary amenorrhea. DESIGN:Case series. SETTING:Pediatric endocrinology, endocrinology, and gynecology departments of academic hospitals. PATIENT(S):Three adolescents and one young woman 46, XY patients with srd5A2 gene mutations. MAIN OUTCOME MEASURE(S):Genetic analysis ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2010.08.007
更新日期：2011-02-01 00:00:00
abstract：:The histologic appearance of the testes of men exposed to chronic gonadotropin-releasing hormone agonist (GnRH-A) therapy has not been previously documented. Herein, we report on the histologic features of the testes of four patients with disseminated prostatic carcinoma who received at least 1 year of daily treatment...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)48205-1
更新日期：1984-11-01 00:00:00
abstract：OBJECTIVE:To establish an intracytoplasmic sperm injection treatment program for couples with male infertility and to determine those factors important for success. DESIGN:A retrospective analysis of 171 consecutive cycles of intracytoplasmic sperm injection concerning 145 infertile couples. SETTING:Infertility clini...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)57489-5
更新日期：1995-04-01 00:00:00
abstract：OBJECTIVE:To report a case of tubal pregnancy in a unicornuate uterus with rudimentary horn on the side of the rudiment and its pathology. DESIGN:Case report. SETTING:University hospital. PATIENT:An 18-year-old woman, primigravida, with tubal pregnancy. INTERVENTION(S):Systemic administration of methotrexate, salpi...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(99)00212-5
更新日期：1999-08-01 00:00:00
abstract：OBJECTIVE:To report a rare case of somatic mosaicism with a germline component of campomelic dysplasia in a woman undergoing in vitro fertilization with preimplantation genetic diagnosis (IVF-PGD). DESIGN:Case report. SETTING:Clinic. PATIENT(S):A 28-year old G2P0110 and her 34-year old husband had two previous pregn...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2018.05.002
更新日期：2018-09-01 00:00:00
abstract：:This Committee Opinion reviews the unresolved issues and experimental nature associated with the procedure and its limited success rates. ...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
doi：10.1016/j.fertnstert.2008.08.033
更新日期：2008-11-01 00:00:00
abstract：OBJECTIVE:To test the hypothesis that an acute reduction in circulating insulin affects LH secretion in women with polycystic ovary syndrome (PCOS). DESIGN:Prospective study in normal and PCOS women. SETTING:General Clinical Research Centers at the University of Virginia, the Medical College of Virginia, and the Mass...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2006.11.109
更新日期：2007-07-01 00:00:00
abstract：:Transvaginal follicular aspiration (TVA) with ultrasonically guided needles allows the transfer of in vitro generated embryos to the fallopian tubes (TET), performing only one surgical procedure in the process. Up to now, this approach has been used to treat 16 couples with infertility due to severe male factor. Folli...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)60136-x
更新日期：1988-09-01 00:00:00
abstract：OBJECTIVE:To correlate histology with endometriosis characteristics. DESIGN:Secondary data analysis. SETTING:Government research hospital. PATIENT(S):One hundred thirty-three women with chronic pelvic pain and endometriosis who underwent laparoscopic surgery between 1999 and 2004. INTERVENTION(S):Laparoscopic excis...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2007.05.042
更新日期：2008-06-01 00:00:00
abstract：OBJECTIVE:To study the hormonal regulation of primary and recurrent endometriosis by comparing the levels of estrogen receptors (ER) and progesterone receptors (PR) with the levels in uterine endometrium obtained simultaneously. DESIGN:Tissue samples collected at routine operations. SETTING:One university clinic. PA...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1993-07-01 00:00:00
abstract：OBJECTIVE:To assess whether seminal vesicle sperm aspiration (SVSA) is an option for wounded warriors with severe genital and testicular injuries, with the goal of cryopreservation to use in future assisted reproductive technology (ART) cycles. DESIGN:Retrospective case series. SETTING:Tertiary care military hospital...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2016.05.028
更新日期：2016-09-01 00:00:00
abstract：:The human placenta in vitro releases luteinizing hormone-releasing factor (LRF) which is immunologically indistinguishable from hypothalamic LRF. The release of LRF by placental tissue increased during the 4 days of culture and exceeded the original tissue content more than 5-fold. These data suggest that the human pl...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)44236-6
更新日期：1979-09-01 00:00:00
abstract：OBJECTIVE:To investigate the role(s) of colony-stimulating factor 1 (CSF-1) on the development of early endometriosis in a murine model by comparing rate of lesion formation in mice [1] homozygous for a CSF-1 mutation versus syngeneic controls and [2] after treatment with imatinib, a commercially available tyrosine kin...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2008.09.050
更新日期：2010-01-01 00:00:00
abstract：OBJECTIVE:To report a case of a methotrexate (MTX)-resistant heterotopic interstitial pregnancy in which dactinomycin was necessary as a second-line chemotherapy. DESIGN:Case report. SETTING:Medical college-affiliated hospital. PATIENT(S):A 39-year-old woman with vaginal bleeding after the transfer of two thawed 8-c...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2008.10.003
更新日期：2009-03-01 00:00:00
abstract：:Where histology used the presence of glands and/or stroma in the myometrium as pathognomonic for adenomyosis, imaging uses the appearance of the myometrium, the presence of striations, related to the presence of endometrial tissue within the myometrium, the presence of intramyometrial cystic structures and the size an...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
doi：10.1016/j.fertnstert.2018.01.006
更新日期：2018-03-01 00:00:00
abstract：:The concentrations of calcium, magnesium, and zinc were measured by atomic absorption spectrometry. The concentrations of all three cations were found to be strongly correlated with one another and with acidity (pH). Analysis of the relative concentrations in prostatic fluid, split ejaculates, and whole seminal plasma...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)43281-4
更新日期：1978-05-01 00:00:00
abstract：OBJECTIVE:To evaluate the safety and efficacy of an antiprogesterone (mifepristone, RU486; Roussel-Uclaf, Romaineville, France) on endometriosis. DESIGN:An open, prospective clinical trial. SETTING:The clinical practice of an academic faculty. PATIENTS:Nine women with endometriosis were studied. INTERVENTIONS:RU486...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章
doi：10.1016/s0015-0282(16)58022-4
更新日期：1996-01-01 00:00:00
abstract：:Klemetti's birth registry study is put in context of other major studies. Summary advice is offered to practitioners as to what they should tell parents who conceive with assisted reproductive technology. ...
journal_title：Fertility and sterility
pub_type： 评论,信件
doi：10.1016/j.fertnstert.2005.04.067
更新日期：2005-11-01 00:00:00
abstract：OBJECTIVE:To evaluate the efficacy of blastocyst culture and transfer in human in vitro fertilization (IVF) as compared to day 3 embryo transfer. DESIGN:Prospective randomized trial. SETTING:Private assisted reproduction unit. PATIENT(S):A total of 162 IVF patients were included in the day 3 embryo transfer (n = 82)...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0015-0282(01)02939-9
更新日期：2002-01-01 00:00:00
abstract：:In response to large numbers of requests for laparoscopic sterilization, the University of North Carolina began a training program to provide this service on a regional basis throughout the state. This report reviews the final distribution of centers providing these services: one within 50 miles of every person in the...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)42312-5
更新日期：1977-01-01 00:00:00
abstract：OBJECTIVE:To report an update on the role of vitamin D (VD) in ovarian physiology with a focus on genes involved in steroidogenesis, follicular development, and ovarian reserve, as well as ovulatory dysfunction associated with polycystic ovary syndrome (PCOS), and ovarian response to assisted reproductive technology (A...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
doi：10.1016/j.fertnstert.2014.04.046
更新日期：2014-08-01 00:00:00
abstract：:Eighty-seven patients who underwent a late secretory phase endometrial biopsy while taking clomiphene citrate (CC) for ovulation induction were studied. Of the endometrial biopsies, 21 (24%) showed an endometrium greater than 2 days out of phase (OOP) with respect to the subsequent menstrual cycle. All 87 patients wer...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)60726-4
更新日期：1989-06-01 00:00:00
abstract：:Anatomic distances along retroperitoneal, inguinal, and infrainguinal segments of the vas deferens were measured in 14 formalin fixed cadavers and in 15 recently postmortem males. There were no significant differences in segment lengths between the two groups nor between the right and left vasa. Data from the recent p...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)53959-4
更新日期：1990-11-01 00:00:00
abstract：:The alkaloids ergocornine, vinblastine, and colcemide, with reported effects interfering with ovum implantation and development were investigated in the white New Zealand rabbit and the macaque monkey. 3 antimetabolites, 5-fluorouracil, 6-azauridine, and BW 57-323H, the 6 mercaptopurine derivative, were also investig...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)36181-7
更新日期：1967-01-01 00:00:00
