Abstract：
OBJECTIVE:To investigate the effect of vitrification and beta-mercaptoethanol (beta-ME) on reactive oxygen species (ROS) activity and in vitro development of oocytes vitrified before or after in vitro fertilization (IVF). DESIGN:Randomized prospective study. SETTING:University-based assisted reproductive technology laboratory. ANIMALS(S): Abattoir-derived porcine ovaries. INTERVENTIONS(S):Oocytes were vitrified either before or 4 hours after the end of IVF by solid surface vitrification (SSV) without centrifugation and/or delipation procedure. beta-ME was used to inhibit ROS activity. MAIN OUTCOME MEASURES(S):Viability was evaluated by membrane integrity and esterase enzyme activity using fluorescein diacetate staining while ROS activity was assessed by 2',7'-dichlorofluorescein assay. RESULT(S):Vitrification increased the ROS activity and decreased the viability and in vitro development of vitrified oocytes. Addition of beta-ME to vitrification and culture medium partially annihilated the ROS activity but did not improve the viability of vitrified-warmed oocytes. Furthermore, beta-ME had no effect on improving the fertilization ability of oocytes vitrified at metaphase II stage but significantly increased their ability to cleave. beta-ME also increased the rate of cleavage and blastocyst formation ability of oocytes vitrified 4 hours after the end IVF. CONCLUSION(S):Vitrification increases ROS activity in oocytes that can be partially annihilated by beta-ME to obtain enhanced embryonic development.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Gupta MK,Uhm SJ,Lee HTdoi
10.1016/j.fertnstert.2010.01.043subject
Has Abstractpub_date
2010-05-15 00:00:00pages
2602-7issue
8eissn
0015-0282issn
1556-5653pii
S0015-0282(10)00103-2journal_volume
93pub_type
杂志文章abstract：OBJECTIVE:To discuss, on the basis of the experience of two clinical cases and extensive literature review, the significance of extremely low levels of anti-Müllerian hormone (AMH), also known as Müllerian-inhibiting substance, in infertile women. DESIGN:Case report. SETTING:University-based infertility clinic at a m...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
doi：10.1016/j.fertnstert.2007.03.084
更新日期：2008-03-01 00:00:00
abstract：:The serum levels of bioactive luteinizing hormone (LH), immunoreactive LH, follicle-stimulating hormone, androstenedione (A), and testosterone (T) were determined in nine anovulatory women with polycystic ovarian syndrome (PCOS) before and after laparoscopic ovarian cautery. Eight ovulated spontaneously and three conc...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)53451-7
更新日期：1990-04-01 00:00:00
abstract：:One hundred four consecutive men who underwent a semen analysis as part of their infertility evaluation were assayed for sperm-associated immunoglobulin (Ig) and the third component of complement (C3). Sixteen (15%) of the 104 men had increased IgG, and 10 (12%) of 86 men tested had increased IgA on their sperm; howev...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1983-04-01 00:00:00
abstract：OBJECTIVE:To describe laparoscopic management of a case of leyomiomatosis peritonealis disseminata associated with ovarian endometriosis. DESIGN:Surgical video article. SETTING:Academic hospital. PATIENT:We present a case of a 30-year-old woman referred to our clinic for abdominal and pelvic pain and dyspareunia. A ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2019.03.023
更新日期：2019-06-01 00:00:00
abstract：OBJECTIVE:To determine whether there is an association between abnormal semen parameters and occupational exposures to organic solvents, metals, and pesticides. DESIGN:Case-control study using three case groups based on different cutoff values for semen parameters and one standard reference group. SETTING:University ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(98)00542-1
更新日期：1999-04-01 00:00:00
abstract：OBJECTIVE:To quantify the effect of the new 2010 World Health Organization (WHO) semen analysis reference values on reclassifying previous semen analysis parameters and definition of patients with male factor infertility. DESIGN:A multi-institutional retrospective chart review. SETTING:University and private male inf...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2012.07.1130
更新日期：2012-12-01 00:00:00
abstract：OBJECTIVE:Alterations in adrenocortical biosynthesis, as measured by the steroid response to acute adrenocorticotropic hormone (ACTH) stimulation, have frequently been reported in female hyperandrogenism. These patients are also commonly obese, which may account for some of these abnormalities. The object of this study...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1991-09-01 00:00:00
abstract：:In patients with polycystic ovarian disease (PCOD) ovulation was induced with a combination of human menopausal gonadotropin (hMG) and human chorionic gonadotropin (hCG) or with urinary follicle-stimulating hormone (uFSH; Metrodin, Serono Laboratories, Inc., Randolph, MA) alone. hMG/hCG and uFSH resulted in comparable...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)48551-1
更新日期：1985-05-01 00:00:00
abstract：:Human menopausal gonadotropins (hMG) and clomiphene citrate (CC), either alone or in combination, are frequently used for in vitro fertilization (IVF) in an attempt to maximize the number of oocytes recovered and the number of embryos transferred. However, direct comparison of the relative efficacy of these protocols ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)49889-4
更新日期：1986-12-01 00:00:00
abstract：:Inflammatory exudates around intrauterine devices were found to produce large concentrations of prostaglandin F2alpha. To evaluate the effect of this substance, 100 semen specimens were incubated in concentrations 100 times greater than that found in normal semen. Motility indices were calculated, and significant inhi...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1977-01-01 00:00:00
abstract：:The present case documents normalization of ovarian perfusion after conservative laparoscopic treatment of adnexal torsion. At our institution, Doppler flow assessment in cases of suspected torsion represents an important element of diagnosis and, in this case, allowed for early diagnosis with conservative laparoscopi...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)56535-2
更新日期：1994-02-01 00:00:00
abstract：OBJECTIVE:To visualize by microhysteroscopy any possible lesions on the endocervix and endometrium made by the catheters commonly used for embryo transfer (ET). DESIGN:Prospective descriptive study. Tertiary fertility center (IFER). PATIENT(S):Twenty-three infertile patients underwent a mock transfer before a microhy...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(03)00607-1
更新日期：2003-08-01 00:00:00
abstract：:Ham's F-10 medium (Gibco, Grand Island, NY) and medium T6 with and without 15% fetal calf serum (FCS) were compared for their ability to support development of murine blastocysts with the capacity to implant and produce normal fetuses when transferred to pseudopregnant females. All media supported equal rates of blast...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)59545-4
更新日期：1987-11-01 00:00:00
abstract：STUDY OBJECTIVE:To determine the effect of baseline complex ovarian cysts on controlled ovarian hyperstimulation and in vitro fertilization (IVF) outcome. DESIGN:Retrospective analysis with stratification by stimulation regimen and the presence or absence of surgically documented endometriosis. PATIENTS:Two hundred s...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1992-06-01 00:00:00
abstract：:Antiphospholipid syndrome patients with recurrent miscarriage have an impairment in fibrinolysis demonstrated by prolonged clot lysis time (CLT) that cannot be attributed to differences in thrombin activatable fibrinolysis inhibitor (TAFI) antigen levels. Patients with unexplained recurrent miscarriage have an impairm...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2010.02.032
更新日期：2010-11-01 00:00:00
abstract：OBJECTIVE:To establish a nonhuman primate model addressing follicular development in cryopreserved prepubertal ovarian tissue after xenografting. DESIGN:Experimental study. SETTING:Academic research center. ANIMAL(S):Ovarian tissue from female prepubertal common marmoset (Callytrix jacchus jacchus) grafted into immu...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2010.11.003
更新日期：2011-03-15 00:00:00
abstract：:A preliminary report on the effect of the addition of caffeine to 45 frozen sperm samples, in order to restore the effectiveness and motility of frozen stored human sperm, is presented. The addition of 7.2 mM caffeine proved optimal and resulted in 40% to 80% increased sperm motility after 30 days of frozen storage. T...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)42378-2
更新日期：1977-02-01 00:00:00
abstract：:In this observational, case-control study in infertile women, we observed elevated serum oxidative stress that was significantly correlated with an increase in serum paraoxonase-1 activity and without any evidence of a proinflammatory reaction. These results suggest a protective role of paraoxonase-1 against inflammat...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2009.11.043
更新日期：2010-08-01 00:00:00
abstract：OBJECTIVE:To modify Tarkowski's air-dry technique for mouse oocytes to develop a rapid, consistent procedure for human oocytes that enables accurate scoring of meiotic stage. DESIGN, SETTING, AND PATIENTS:Meiotically immature human oocytes, obtained after oophorectomy, were cultured for various periods and then subjec...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)55021-3
更新日期：1992-05-01 00:00:00
abstract：OBJECTIVE:To investigate the relative differences in outcomes among microdissection testicular sperm extraction (micro-TESE), conventional testicular sperm extraction (cTESE), and testicular sperm aspiration (TESA) in men with nonobstructive azoospermia. DESIGN:Systematic review and meta-analysis. SETTING:Outpatient ...
journal_title：Fertility and sterility
pub_type： 杂志文章,meta分析,评审
doi：10.1016/j.fertnstert.2015.07.1136
更新日期：2015-11-01 00:00:00
abstract：OBJECTIVE:The purpose of the study was to describe and to compare the rate of rise of human chorionic gonadotropin (hCG) in vanishing twin and normally progressing twin pregnancies during the first trimester. DESIGN:All patients with twin pregnancies between 1985 and 1989 were prospectively studied. Human chorionic go...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)54475-6
更新日期：1991-08-01 00:00:00
abstract：OBJECTIVE:To test by genomic analysis whether empty follicle syndrome (EFS) in a family with two affected sisters has a genetic basis. DESIGN:Whole-exome sequencing in the context of clinical genetics. SETTING:University hospital. PATIENT(S):Two women (36 and 32 years old at the time of the study) with EFS. INTERVE...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2011.05.057
更新日期：2011-08-01 00:00:00
abstract：OBJECTIVE:To describe an ovarian abscess presenting very late after oocyte retrieval for IVF with several unusual clinical features. DESIGN:Case report. SETTING:Academic medical center. PATIENT(S):A 35-year-old nulliparous woman underwent IVF with uncomplicated transvaginal oocyte retrieval (TVOR), resulting in a di...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2005.12.045
更新日期：2006-07-01 00:00:00
abstract：:Eight premenopausal women with cancer had blood drawn for analysis of brain-derived neurotrophic factor (BDNF) and müllerian-inhibiting substance (MIS) before and 3 months after receiving chemotherapy. Unlike MIS, BDNF levels were not reduced after chemotherapy. ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2010.10.033
更新日期：2011-04-01 00:00:00
abstract：:The effects of maternal preovulatory serum and human fetal cord serum supplement in culture medium in human in vitro fertilization and embryo development were compared in 208 cases over an 8-month period when there were no significant changes in other variables. A significantly higher pregnancy rate was observed in th...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)47537-0
更新日期：1984-01-01 00:00:00
abstract：OBJECTIVE:To report the successful and feasible use of transperineal fine needle aspiration of seminal vesicles (FNASV) for sperm retrieval in obstructive azoospermia. DESIGN:Case report. SETTING:Outpatient care in institutional clinic. PATIENT(S):A 31-year-old man with obstructive azoospermia due to a middle prosta...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2006.03.069
更新日期：2006-12-01 00:00:00
abstract：:The predictive value of the metoclopramid-provocation test to detect transient hyperprolactinemia during cycle stimulation was assessed. Patients developing hyperprolactinemia during cycle stimulation showed a significantly higher PRL response to the metoclopramid-provocation test before cycle stimulation. The current...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)53300-7
更新日期：1990-02-01 00:00:00
abstract：OBJECTIVE:To evaluate the effect of metformin in polycystic ovary syndrome (PCOS). As follow-up on a previous paper describing hormonal and metabolic factors, this paper focuses on correlations between adiponectin and anthropometric, hormonal, and metabolic factors in PCOS and the effect of metformin. DESIGN:Randomize...
journal_title：Fertility and sterility
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.fertnstert.2010.01.057
更新日期：2010-11-01 00:00:00
abstract：OBJECTIVE:To determine the feasibility of performing in vivo magnetic resonance elastography (MRE) for uterine leiomyoma. DESIGN:Pilot study. SETTING:Academic medical center. PATIENT(S):Six subjects planning surgical excision of uterine leiomyomas. INTERVENTION(S):MRE before planned surgery. MAIN OUTCOME MEASURE(S...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章
doi：10.1016/j.fertnstert.2010.06.004
更新日期：2011-01-01 00:00:00
abstract：OBJECTIVE:To evaluate the effectiveness of sublingual misoprostol for cervical ripening before diagnostic hysteroscopy in premenopausal women. DESIGN:Placebo-controlled, double-blind, randomized trial. SETTING:University hospital. PATIENT(S):Fifty-two women with an indication for diagnostic hysteroscopy. INTERVENTI...
journal_title：Fertility and sterility
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.fertnstert.2009.01.073
更新日期：2010-05-01 00:00:00
