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The effect of preimplantation culture conditions on murine embryo implantation and fetal development.

Abstract：

:Ham's F-10 medium (Gibco, Grand Island, NY) and medium T6 with and without 15% fetal calf serum (FCS) were compared for their ability to support development of murine blastocysts with the capacity to implant and produce normal fetuses when transferred to pseudopregnant females. All media supported equal rates of blastocyst development from 2-cell embryos. In addition, there were no differences in the rates of blastocyst implantation. However, once implanted, blastocysts grown in T6 produced a significantly higher proportion of normal fetuses (58.5% to 65.9%) than blastocysts grown in either Ham's F-10 (2.4%) or T6 with FCS (27.6%). These results demonstrate that the rate of murine blastocyst development from 2-cell embryos in vitro is not a good criterion of healthy embryos. Murine blastocysts transferred in medium with 0% versus 50% FCS implanted and developed into normal fetuses at equal rates.

journal_name

Fertil Steril

journal_title

Fertility and sterility

authors

Arny M,Nachtigall L,Quagliarello J

doi

10.1016/s0015-0282(16)59545-4

subject

Has Abstract

pub_date

1987-11-01 00:00:00

pages

861-5

issue

5

eissn

0015-0282

issn

1556-5653

pii

S0015-0282(16)59545-4

journal_volume

48

pub_type

杂志文章

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