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Assessing loss of imprint methylation in sperm from subfertile men using novel methylation polymerase chain reaction Luminex analysis.

Abstract：

OBJECTIVE:To assess the clinical value of bisulfite polymerase chain reaction Luminex (BPL), an automated, high-throughput procedure for the detection of alterations in DNA methylation. DESIGN:Experimental prospective study. SETTING:University research laboratory and private in vitro fertilization (IVF) clinic. PATIENT(S):A total of 337 men, 61 with severe oligozoospermia, 67 with moderate oligozoospermia, and 209 with microscopically normozoospermia. INTERVENTION(S):The ejaculated sperm samples after the routine semen analysis with patients' consent. MAIN OUTCOME MEASURE(S):Examination of the methylation patterns of eight imprinted loci in sperm DNA, and confirmation with combined bisulfite PCR restriction analysis (COBRA). RESULT(S):A total of 47 cases (13.9%) showed abnormal methylation at one or more imprinted loci (18 paternal, 18 maternal, and 11 cases with alterations of both maternal and paternal imprints). CONCLUSION(S):The relative ease of the BPL method provides a practical method within a clinical setting to reduce the likelihood of abnormal samples being used in assisted reproduction treatments.

journal_name

Fertil Steril

journal_title

Fertility and sterility

authors

Sato A,Hiura H,Okae H,Miyauchi N,Abe Y,Utsunomiya T,Yaegashi N,Arima T

doi

10.1016/j.fertnstert.2010.06.076

subject

Has Abstract

pub_date

2011-01-01 00:00:00

pages

129-34, 134.e1-4

issue

1

eissn

0015-0282

issn

1556-5653

pii

S0015-0282(10)01029-0

journal_volume

95

pub_type

临床试验,杂志文章

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