Abstract:
OBJECTIVE:To evaluate the potential clinical application of Raman spectroscopy (RS) as a tool that may identify spermatogenesis within human seminiferous tubules. DESIGN:RS scanning of human testicular tissue at different maturational stages; immunohistochemistry study and metabolomic analysis of nonobstructive azoospermic/obstructive azoospermic testes. SETTING:State-owned hospital. PATIENT(S):Fifty-two patients with clinical indications of nonobstructive azoospermia (NOA) and obstructive azoospermia (OA) who underwent infertility evaluation and treatment. INTERVENTION(S):None. MAIN OUTCOME MEASUREMENT(S):Raman spectra of seminiferous tubules, thickness of lamina propria (LP), immunohistochemistry of type I, III, and IV collagens and laminin, metabolites of human testes. RESULT(S):Tubules of OA patients had spectral intensities below 2,000 (au), while tubules of NOA patients had higher intensities, depending on the degree of spermatogenesis. RS was able to separate samples of NOA and OA testicular tissue with a sensitivity of 90% and specificity of 85.71%. The LP of NOA tubules were thickened and had increased deposition of type I and type III collagens. Gas chromatography-mass spectrometer (GC-MS) detected 12 metabolites that showed significant differences between NOA and OA testes. CONCLUSION(S):RS can noninvasively distinguish seminiferous tubules with complete and incomplete spermatogenesis and may serve as a novel and potentially useful tool to guide surgeons performing micro-testicular sperm extraction to improve sperm retrieval.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Liu Y,Zhu Y,Di L,Osterberg EC,Liu F,He L,Hu H,Huang Y,Li PS,Li Zdoi
10.1016/j.fertnstert.2014.03.035subject
Has Abstractpub_date
2014-07-01 00:00:00pages
54-60.e2issue
1eissn
0015-0282issn
1556-5653pii
S0015-0282(14)00290-8journal_volume
102pub_type
杂志文章abstract::This document discusses the evaluation and management of varicoceles in the male partners of infertile couples, and presents the controversies and recommendations regarding this condition. This document replaces the ASRM Practice Committee document titled "Report on Varicocele and Infertility," last published in 2008,...
journal_title:Fertility and sterility
pub_type: 杂志文章,实务指引
doi:10.1016/j.fertnstert.2014.10.007
更新日期:2014-12-01 00:00:00
abstract:OBJECTIVE:To propose the sperm recovering efficiency (SRE) model, an equation that can be used to objectively quantify the technical skill of individual operators processing semen specimens in an andrology laboratory. Additionally, this equation can be used to compare different semen processing methods and protocols. T...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(03)00253-x
更新日期:2003-06-01 00:00:00
abstract:OBJECTIVE:To investigate whether antiphospholipid and related autoantibodies are associated with IVF implantation failure as well as with recurrent spontaneous miscarriage. DESIGN:Prevalence study. SETTING:University teaching hospital and associated IVF unit. PATIENT(S):Patients with at least three consecutive first...
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章
doi:10.1016/s0015-0282(98)00312-4
更新日期:1998-11-01 00:00:00
abstract::Meta-analyses have become an increasingly popular method of drawing conclusions when there are multiple publications addressing a particular topic. While the statistical calculations are straightforward, many of the decisions regarding the research question, populations of interest, and inclusion/exclusion criteria ar...
journal_title:Fertility and sterility
pub_type: 杂志文章,评审
doi:10.1016/j.fertnstert.2011.05.029
更新日期:2011-07-01 00:00:00
abstract:OBJECTIVE:To analyze embryo morphology in relation to the corresponding chromosomal status, in order to evaluate the effects of aneuploidy over fragmentation, delayed cleavage, and arrested cleavage. DESIGN:Retrospective study. SETTING:Reproductive Medicine Unit, Società Italiana di Studi di Medicina della Riproduzio...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2006.07.1512
更新日期:2007-03-01 00:00:00
abstract:OBJECTIVE:To examine the histopathological effects of diabetes on spermatogenesis and to detect the proliferation of germ cells with proliferative cell nuclear antigen (PCNA). DESIGN:Controlled experimental study. SETTING:The animal laboratory of the Experimental Surgery Center of Ege University, Izmir, Turkey. ANIM...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(03)00984-1
更新日期:2003-09-01 00:00:00
abstract::This study indicates that SP occur in a higher percentage than theoretically expected after IVE-ET treatment, but it is not conclusive as to whether the SP is independent or a result of the treatment or operative laparoscopy. Our results are in agreement with those of previous studies that have shown that SP occur wit...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)49289-7
更新日期:1986-04-01 00:00:00
abstract:OBJECTIVE:To review MEDLINE literature for correlations between insulin resistance and erectile dysfunction (ED). DESIGN:MEDLINE literature review (1966 to present). SETTING:Academic medical center. PATIENT(S):None. INTERVENTION(S):None. MAIN OUTCOME MEASURE(S):None. RESULT(S):Erectile dysfunction affects more th...
journal_title:Fertility and sterility
pub_type: 杂志文章,评审
doi:10.1016/j.fertnstert.2007.01.116
更新日期:2007-10-01 00:00:00
abstract:OBJECTIVE:To characterize and localize interleukin (IL)-1alpha and IL-1beta in human sperm cells. DESIGN:Prospective and comparative study. SETTING:Andrology clinic of a university hospital. PATIENT(S):Two groups of normogonadotropic men: 17 fertile men (donors with proved fertility) and 8 oligoteratoasthenospermic ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(00)00499-4
更新日期:2000-06-01 00:00:00
abstract::This study investigated the prevalence of Tyr204Phe and Val34Leu polymorphisms in two Brazilian ethnic groups (171 Caucasians and 27 Blacks, and 117 men and 81 women) and in patients with recurrent miscarriages (RM) (86 women: 53 Caucasians and 33 Blacks). Study groups were matched to control groups by race and age. T...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2004.04.052
更新日期:2004-11-01 00:00:00
abstract::The data from 83 consecutive in vitro fertilization-embryo transfer cycles were examined with emphasis on the presence of subfertile male parameters, including abnormal sperm density, motility, morphologic features, or an abnormal result in the sperm penetration assay (zona-free hamster ova penetration). There were 25...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)49338-6
更新日期:1986-05-01 00:00:00
abstract:OBJECTIVE:To confirm successful implantation of IVF, cryopreserved human embryos after assisted hatching with acidic Tyrode's solution. DESIGN:Case report. SETTING:In vitro fertilization-ET facility of a university-based practice. PATIENT:A 28-year-old female with nonoperable bilateral tubal occlusion and > 1 1/2 ye...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)57376-2
更新日期:1995-02-01 00:00:00
abstract:OBJECTIVE:To evaluate the effects on the lipid pattern and insulin sensitivity of hirsute women of an oral contraceptive pill containing 30 microg of ethinyl estradiol and 150 microg of desogestrel. DESIGN:Prospective clinical study. SETTING:Tertiary care institutional hospital. PATIENT(S):16 hirsute women. INTERVE...
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章
doi:10.1016/s0015-0282(00)00718-4
更新日期:2000-10-01 00:00:00
abstract::Fifty patients have been sterilized laparoscopically using a plastic tubal (Bleier) clip having a "snap-shut" mechanism. Initial assessment indicates that the method is simple in principle but there are limitations, including the bulky dimensions of the applicator, absence of a safety-catch mechanism, and faults in th...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)44111-7
更新日期:1979-07-01 00:00:00
abstract:OBJECTIVE:To determine the presence of M. genitalium and C. trachomatis in women attending fertility clinics and to follow these women for the effects of previous infections or tubal damage on pregnancy rate and outcome. DESIGN:Prospective study. SETTING:Fertility clinics and university. PATIENT(S):Two hundred twelv...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2006.12.056
更新日期:2008-09-01 00:00:00
abstract:OBJECTIVE:To compare the performance of the SureView catheter, a new ultrasonic embryo transfer (ET), with the classic Wallace catheter during ultrasound-guided ET. DESIGN:Prospective, randomized study. SETTING:Private, academically affiliated clinic. PATIENT(S):175 patients undergoing donor egg in vitro fertilizati...
journal_title:Fertility and sterility
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.fertnstert.2009.01.090
更新日期:2010-02-01 00:00:00
abstract::Pelvic adhesion formation represents a major problem following fallopian tube surgery for infertility. Intraperitoneal dextran may prevent pelvic adhesions. Extensive personal clinical experience (W. H. U.) with intraperitoneal dextran organ-flotation on completion of tubal and ovarian surgery has appeared to limit ad...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)43765-9
更新日期:1979-01-01 00:00:00
abstract::The predictive value of the metoclopramid-provocation test to detect transient hyperprolactinemia during cycle stimulation was assessed. Patients developing hyperprolactinemia during cycle stimulation showed a significantly higher PRL response to the metoclopramid-provocation test before cycle stimulation. The current...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)53300-7
更新日期:1990-02-01 00:00:00
abstract:OBJECTIVE:To assess the molecular signatures underlying endometrial disorder using cDNA microarray. DESIGN:Gene expression-based oligonucleotide array of the normal endometrium. SETTING:University hospital. PATIENT(S):Humans. INTERVENTION(S):Endometrial tissues were obtained from 28 normal cycling women undergoing ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2009.01.130
更新日期:2010-06-01 00:00:00
abstract::One hundred forty-seven cycles in normal ovulatory patients are reported. All were stimulated with human menopausal/human chorionic gonadotropin. Three estrogen responses were identified: normal, high, and low. Patients who achieved pregnancy formed a fourth category, the pregnancy group. The number of preovulatory an...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)47590-4
更新日期:1984-02-01 00:00:00
abstract:OBJECTIVE:To determine if pentoxifylline has an in vitro effect on human sperm motility in subjects with normal semen analysis. DESIGN:Prospective, longitudinal study of the effect of pentoxifylline on normospermic semen samples using computerized analysis. SETTING:The Assisted Conception Unit, Royal Maternity Hospit...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)55708-2
更新日期:1993-02-01 00:00:00
abstract:OBJECTIVE:To determine whether cognitive behavior therapy (CBT) targeted to problematic attitudes common among women with functional hypothalamic amenorrhea would restore ovarian function. DESIGN:Randomized, prospective, controlled intervention. SETTING:Clinical research center in an academic medical institution. PA...
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/s0015-0282(03)01124-5
更新日期:2003-10-01 00:00:00
abstract::Serum and peritoneal fluid from five fertile women without endometriosis and serum (n = 23) and peritoneal fluid (n = 12) from infertile women with endometriosis were tested for the presence of antibodies against endometrial tissue antigens by a Western blot analysis. Antigens with molecular weights (MW) of 19, 31, 38...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)60362-x
更新日期:1988-12-01 00:00:00
abstract:OBJECTIVE:To investigate the role of heart and neural crest derivatives-expressed transcript 2 (HAND2) during decidualization of human endometrial stromal cells (ESCs). DESIGN:In vitro experiment. SETTING:Research laboratory. PATIENT(S):Twenty-six patients undergoing hysterectomy for benign reasons. INTERVENTION(S)...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2014.03.013
更新日期:2014-06-01 00:00:00
abstract:OBJECTIVE:To investigate differentially expressed genes in epithelial and stromal cells of eutopic endometrium from patients with deep endometriosis and women with normal pelvic cavities using laser capture microdissection and complementary DNA microarrays. DESIGN:Prospective study. SETTING:University hospital. PATI...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2005.04.041
更新日期:2005-10-01 00:00:00
abstract::We found that four polymorphisms in the protein L-isoaspartyl-O-methyltransferase (PCMT1) gene, encoding a protein repair enzyme, are associated with premature ovarian failure (POF). All four polymorphisms were in strong linkage disequilibrium. The frequencies of two haplotypes were statistically significantly differe...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2008.03.078
更新日期:2009-04-01 00:00:00
abstract::Where histology used the presence of glands and/or stroma in the myometrium as pathognomonic for adenomyosis, imaging uses the appearance of the myometrium, the presence of striations, related to the presence of endometrial tissue within the myometrium, the presence of intramyometrial cystic structures and the size an...
journal_title:Fertility and sterility
pub_type: 杂志文章,评审
doi:10.1016/j.fertnstert.2018.01.006
更新日期:2018-03-01 00:00:00
abstract:OBJECTIVE:To evaluate endometrial adhesion and invasion of peritoneal mesothelium. DESIGN:Descriptive study using confocal laser-scanning microscopy. SETTING:University-based laboratory. PATIENT(S):Women undergoing surgery for benign conditions. INTERVENTION(S):Fluorescence-labeled peritoneal mesothelial cells (PMC...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(02)04834-3
更新日期:2003-03-01 00:00:00
abstract:OBJECTIVE:To present a case report of a unilateral triplet ectopic pregnancy (EP) conceived by IVF-embryo transfer. DESIGN:Case report. SETTING:University Hospital, Budapest, Hungary. PATIENT(S):A 26-year-old infertile woman with a history of right salpingectomy, hyperprolactinemia, and male factor infertility under...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2008.03.018
更新日期:2008-11-01 00:00:00
abstract:OBJECTIVE:To report a case of hyperreactio luteinalis (HL) with elevated levels of human chorionic gonadotropin (hCG) and a severe imbalance of angiogenic factors, which developed into severe, early onset preeclampsia (PE). DESIGN:Case report. SETTING:University perinatal center. PATIENT(S):A 32-year-old primigravid...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2009.04.002
更新日期:2009-07-01 00:00:00