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Factors of importance for the establishment of a successful program of intracytoplasmic sperm injection treatment for male infertility.

Abstract：

OBJECTIVE:To establish an intracytoplasmic sperm injection treatment program for couples with male infertility and to determine those factors important for success. DESIGN:A retrospective analysis of 171 consecutive cycles of intracytoplasmic sperm injection concerning 145 infertile couples. SETTING:Infertility clinic in a private hospital associated with a university hospital. PATIENTS:Couples with infertility in the male partner whose sperm parameters were unacceptable for conventional IVF or in whom fertilization by conventional IVF failed repeatedly. INTERVENTIONS:One hundred seventy-one transvaginal oocyte retrievals were completed after superovulation with GnRH agonist and gonadotropins. MAIN OUTCOME MEASURES:The parameters evaluated included fertilization, cleavage, implantation, pregnancy, and spontaneous abortion in relation to patient indications and improved procedures. RESULTS:After intracytoplasmic sperm injection, normal fertilization occurred in 45% of the oocytes (n = 1,499). Of 171 treatment cycles, 93% of the couples had fertilization and 86% had ET. Thirty-six pregnancies were achieved. During the period studied, the mean fertilization rate increased from 21.3% during the first 17 weeks to 67.8% during the last 13 weeks, and the pregnancy rate (PR) per started cycle increased from 12.8% to 31.3%. CONCLUSIONS:Technical factors critical for achieving high rates of fertilization and pregnancy were the use of standardized intracytoplasmic sperm injection pipettes, the immobilization of sperm before injection, and the aspiration of a minimal amount of ooplasm before reinjection with the sperm. Intracytoplasmic sperm injection appears to be superior to other micromanipulation methods for alleviating male infertility.

journal_name

Fertil Steril

journal_title

Fertility and sterility

authors

Svalander P,Forsberg AS,Jakobsson AH,Wikland M

doi

10.1016/s0015-0282(16)57489-5

subject

Has Abstract

pub_date

1995-04-01 00:00:00

pages

828-37

issue

eissn

0015-0282

issn

1556-5653

pii

S0015-0282(16)57489-5

journal_volume

pub_type

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Factors of importance for the establishment of a successful program of intracytoplasmic sperm injection treatment for male infertility.