Abstract:
OBJECTIVE:To compare levels of apoptosis in granulosa cells from women treated with the gonadotropin-releasing hormone (GnRH) agonist triptorelin or the GnRH antagonist cetrorelix. DESIGN:Randomized, prospective study. SETTING:University hospital. PATIENT(S):Thirty-two women undergoing assisted reproduction techniques after ovulation induction with recombinant follicle-stimulating hormone (FSH) plus GnRH agonist or antagonist. INTERVENTION(S):Granulosa cells were isolated from follicular aspirates after oocyte removal. MAIN OUTCOME MEASURE(S):Apoptosis was assessed with Annexin V binding assay, terminal deoxynucleotidyl transferase (TdT)-mediated nick-end labeling (TUNEL) assay, flow cytometric analysis of DNA, and ultrastructural analysis of cell morphology in transmission electron microscopy. Serum and follicular hormonal levels were also determined. RESULT(S):Annexin V binding and TUNEL assays revealed comparable percentages of apoptosis in the two groups under investigation. Analysis of DNA histograms revealed a similar cell cycle distribution in the two groups. Ultrastructural analysis only occasionally displayed patterns of chromatin margination in apoptotic cells. The mean concentrations of all the follicular fluid steroid hormones evaluated (E2, T, and P) were significantly lower in the GnRH antagonist-treated group. CONCLUSION(S):Therapy with a GnRH agonist or antagonist is associated with comparable levels of apoptosis in granulosa cells.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Giampietro F,Sancilio S,Tiboni GM,Rana RA,Di Pietro Rdoi
10.1016/j.fertnstert.2005.08.020subject
Has Abstractpub_date
2006-02-01 00:00:00pages
412-9issue
2eissn
0015-0282issn
1556-5653pii
S0015-0282(05)03870-7journal_volume
85pub_type
杂志文章,随机对照试验abstract::The hormonal profile of the aging male reveals an associated decrease in free testosterone and 5 alpha-dihydrotestosterone (DHT) levels and increased luteinizing hormone levels. Later events consist of a decrease in total testosterone, stable DHT, and increased follicle-stimulating hormone and estradiol levels. Althou...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)41958-8
更新日期:1976-07-01 00:00:00
abstract:OBJECTIVE:To elucidate reinitiation factors in human spermatozoa preserved in the electrolyte-free solution at 4 degrees C. SETTING:Andrology laboratory of our hospital. PATIENTS:Semen samples were obtained from patients attending our infertility clinic. INTERVENTIONS:Ejaculated sperm were centrifuged and washed usi...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)58341-1
更新日期:1996-06-01 00:00:00
abstract:OBJECTIVE:To study differences between robotic and laparoscopic approach in Essure device removal. DESIGN:Demonstration video of two different surgical approaches. SETTING:Clinico San Carlos University Hospital in Madrid, Spain. PATIENT(S):A total of 737 Essure devices were implanted until June 2017 in Clinico San C...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2020.03.025
更新日期:2020-07-01 00:00:00
abstract::Hormonal profiles were compared in 14 ovulatory women who were treated with two different doses of gonadotropins in successive in vitro fertilization cycles. All patients suffered from mechanical causes of infertility. Serum estradiol (E2), follicle-stimulating hormone (FSH), luteinizing hormone (LH), and progesterone...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:
更新日期:1988-06-01 00:00:00
abstract::Defects of the DNA mismatch repair gene hMLH3 were screened by denaturing high-performance liquid chromatography and sequencing in germinal tissue DNA from patients with spermatogenic arrest, with sequence variations being confirmed in genomic DNA by polymerase chain reaction (PCR) direct sequencing analysis. Four mis...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2007.01.063
更新日期:2007-12-01 00:00:00
abstract:OBJECTIVE:To provide a step-by-step description of our published technique of single-site robot-assisted laparoscopic myomectomy with the goal of promoting its safe adoption. DESIGN:Surgical video tutorial. SETTING:University medical center. PATIENT(S):Ten women undergoing single-site robot-assisted laparoscopic myo...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2015.07.1159
更新日期:2015-11-01 00:00:00
abstract:OBJECTIVE:To compare saline infusion sonography (SIS) with gel instillation sonography (GIS) in terms of feasibility and diagnostic accuracy. DESIGN:Prospective cohort study. SETTING:Leuven University bleeding clinic. PATIENT(S):A total of 804 patients: two consecutive cohorts of 402 women undergoing SIS or GIS. IN...
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章
doi:10.1016/j.fertnstert.2010.04.074
更新日期:2011-01-01 00:00:00
abstract:OBJECTIVE:To test the hypothesis that IVF-ET pregnancy rates (PRs) for patients with tubal factor infertility are decreased in patients with hydrosalpinges and that surgical correction reverses this effect. DESIGN:Retrospective chart review. SETTING:Private practice IVF-ET program. PATIENT(S):Patients (n = 160) unde...
journal_title:Fertility and sterility
pub_type: 杂志文章,评审
doi:10.1016/s0015-0282(97)00447-0
更新日期:1998-01-01 00:00:00
abstract:OBJECTIVE:To assess the impact of embryo retention in the embryo transfer catheter followed by "immediate" retransfer on pregnancy outcome in women undergoing assisted reproduction. DESIGN:Retrospective analysis of embryo transfer following in vitro fertilization. SETTING:Assisted reproductive technology practice in ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2004.01.035
更新日期:2004-08-01 00:00:00
abstract:OBJECTIVE:To compare the low-dose recombinant FSH and hMG protocols in treatment of patients with history of severe ovarian hyperstimulation syndrome (OHSS). DESIGN:A prospective study on 22 patients with history of severe OHSS. Group A (n = 14) was treated with low-dose recombinant FSH 40 cycles and group B (n = 8) w...
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章
doi:10.1016/s0015-0282(16)58631-2
更新日期:1996-11-01 00:00:00
abstract::A study has been carried out, by means of an indole microchemical technique, of the DNA content of spermatozoa from a group of 13 normal subjects and 13 patients affected by infertility, characterized by oligospermia with a clinical, hormonal, and testicular picture peculiar to "idiopathic spermatidic arrest." The DNA...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)41590-6
更新日期:1976-01-01 00:00:00
abstract:OBJECTIVE:To determine the prognostic impact of the nuclear status at the two-cell stage on intracytoplasmic sperm injection (ICSI) outcomes. DESIGN:Retrospective study. SETTING:Hospital. PATIENT(S):Only ICSI cycles with time-lapse monitoring of transferred embryos with known implantation/delivery data from November...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2016.09.022
更新日期:2017-01-01 00:00:00
abstract::In 115 patients (group I) in which normal liquefaction of semen occurred, the fertilization rate of the yielded oocytes was 71.2% after standard in vitro fertilization procedure, and the viable pregnancy rate per patient per cycle was 13.0%. In a comparable group of 26 patients (group II), in which semen liquefaction ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)60444-2
更新日期:1989-01-01 00:00:00
abstract:OBJECTIVE:To provide a detailed summary of current scientific knowledge of somatic stem cells (SSCs) in murine and human myometrium and their putative implication in leiomyoma formation, as well as to establish new therapeutic options. DESIGN:Pubmed and Scholar One manuscripts were used to identify the most relevant s...
journal_title:Fertility and sterility
pub_type: 杂志文章,评审
doi:10.1016/j.fertnstert.2014.04.051
更新日期:2014-09-01 00:00:00
abstract::This document is designed to assist in vitro fertility clinics in the management of cryopreserved reproductive tissues stored in cryogenic storage (cryostorage) tanks, based upon scientific principles and laboratory experience related to best practice for safe and reliable storage of cryopreserved reproductive tissue....
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2020.06.019
更新日期:2020-09-01 00:00:00
abstract:OBJECTIVE:To demonstrate a new approach for performing an ovarian cystectomy via transvaginal natural orifice transluminal endoscopic surgery (vNOTES) as an alternative for a laparoscopic ovarian cystectomy. DESIGN:Stepwise explanation of the technique using original video footage. SETTING:Hospital. PATIENT(S):Fourt...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2017.10.037
更新日期:2018-02-01 00:00:00
abstract:OBJECTIVE:To describe three cases of azoospermia in patients with oligospermia after clomiphene citrate (CC) intake. DESIGN:Case report. SETTING(S):Academic medical center. PATIENT(S):Three patients with oligospermia. INTERVENTION(S):Three oligospermic men used CC, resulting in azoospermia. MAIN OUTCOME MEASURE(S)...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2008.03.036
更新日期:2008-11-01 00:00:00
abstract::Peritoneal fluid and serum were collected from 78 patients at the time of laparoscopy. Twenty-two were fertile controls (CTL), and 56 were infertility patients, who were subdivided into three main groups: endometriosis (EMS), pelvic adhesions (ADH), and ovarian dysfunction (OvDF). Based on control group data, biochemi...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)48020-9
更新日期:1984-08-01 00:00:00
abstract:OBJECTIVE:To compare the expression of the prostaglandin (PG) E(2) transporter multidrug resistance-associated protein 4 (MRP4) in eutopic and ectopic endometrial tissue from endometriosis patients with that of control subjects and to examine whether MRP4 is regulated by the antiinflammatory lipid lipoxin A(4) (LXA(4))...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2013.01.146
更新日期:2013-06-01 00:00:00
abstract:OBJECTIVE:To assess whether IV tramadol before outpatient hysteroscopy could reduce procedure-related pain. DESIGN:A randomized double-blind placebo controlled trial. SETTING:Outpatient Hysteroscopy Centre in the Department of Obstetrics and Gynaecology of Cagliari University. PATIENT(S):Fifty healthy, parous, women...
journal_title:Fertility and sterility
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.fertnstert.2006.05.072
更新日期:2007-01-01 00:00:00
abstract:OBJECTIVE:To compare the mRNA expression of vascular endothelial growth factor (VEGF) 121 and 165 isoforms and thrombospondin-1 (TSP-1) after raloxifene, 17beta-E(2), and P administration in cultured Ishikawa cells. DESIGN:Prospective basic research study. SETTING:Jones Institute for Reproductive Medicine, Eastern Vi...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(03)00350-9
更新日期:2003-06-01 00:00:00
abstract::Twelve adult does in estrus were artificially inseminated with 1 ml of semen from bucks that had received injections of 3H-thymidine 22 to 32 days previously. The ejaculates contained radioactive seminal plasma and unlabeled sperm. The reproductive tracts of the does were removed at selected intervals after inseminati...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)42622-1
更新日期:1977-06-01 00:00:00
abstract::This study compares the quality, developmental capacity and global gene expression profile of embryos generated from superovulated and natural cycles. We found that ovarian stimulation impaired quality of blastocysts (cell number: 68.9 +/- 6.9 vs. 76.8 +/- 7.9; diameter of blastocysts: 145.3 +/- 76.9 microm vs. 152.6 ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2010.03.018
更新日期:2010-05-15 00:00:00
abstract::Most oocyte donation programs have experienced higher pregnancy rates than usually seen in regular in vitro fertilization (IVF), suggesting that the quality of either the oocytes or the endometrium is superior. To clarify this issue we analyzed the results of transfers of 136 cryopreserved embryos originating either f...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)53830-8
更新日期:1990-10-01 00:00:00
abstract:OBJECTIVE:To investigate the relationship between growth hormone (GH) and luteal function. DESIGN:In vivo comparative and in vitro culture studies. SETTING:University hospital. PATIENT(S):Eighteen women who were interested in becoming pregnant and visited our clinic voluntarily participated in the study for urinary ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2007.07.1304
更新日期:2008-09-01 00:00:00
abstract::Eighty-seven patients who underwent a late secretory phase endometrial biopsy while taking clomiphene citrate (CC) for ovulation induction were studied. Of the endometrial biopsies, 21 (24%) showed an endometrium greater than 2 days out of phase (OOP) with respect to the subsequent menstrual cycle. All 87 patients wer...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)60726-4
更新日期:1989-06-01 00:00:00
abstract:OBJECTIVE:To evaluate the potential clinical application of Raman spectroscopy (RS) as a tool that may identify spermatogenesis within human seminiferous tubules. DESIGN:RS scanning of human testicular tissue at different maturational stages; immunohistochemistry study and metabolomic analysis of nonobstructive azoosp...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2014.03.035
更新日期:2014-07-01 00:00:00
abstract:OBJECTIVE:To evaluate spontaneous apoptosis in single-cell suspensions of eutopic and ectopic endometrium from women with endometriosis and in eutopic endometrium from fertile controls without endometriosis. DESIGN:Paired specimens of eutopic and ectopic endometrial tissue from patients with endometriosis and eutopic ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(98)00073-9
更新日期:1998-06-01 00:00:00
abstract:OBJECTIVE:To compare zygote intrafallopian transfer (ZIFT) and in vitro fertilization and embryo transfer (IVF-ET) as treatments of male-factor infertility. DESIGN:Patients were prospectively randomized to ZIFT or IVF-ET. SETTING:In vitro fertilization program of the Centre for Reproductive Medicine of the Dutch-spea...
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/s0015-0282(16)55195-4
更新日期:1992-08-01 00:00:00
abstract:OBJECTIVE:To investigate the potential effects of TAT-PRDX2 protein supplementation to the cryopreservation medium on post-thaw sperm quality and function. DESIGN:In vitro prospective study. SETTING:Medical university hospital. PATIENT(S):Fifty normozoospermic, 50 asthenozoospermic, and 50 oligoasthenozoospermic men...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2018.07.008
更新日期:2018-11-01 00:00:00