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Improved clinical outcomes for in vitro fertilization with delay of embryo transfer from 48 to 72 hours after oocyte retrieval: use of glucose- and phosphate-free media.

Abstract：

OBJECTIVE:To evaluate clinical outcomes of day 2 versus day 3 ET using a culture media with no glucose or phosphate. DESIGN:Retrospective clinical study. SETTING:Hospital-based fertility clinic. PATIENT(S):One hundred seventy-six IVF-ET patients undergoing controlled ovarian supraovulation. INTERVENTION(S):IVF and delaying the ET by 1 day. MAIN OUTCOME MEASURE(S):Number of blastomeres per embryo, implantation and pregnancy rates. RESULT(S):Delaying the ET from day 2 to day 3 after oocyte retrieval significantly increased implantation rates (13% versus 24%) and ongoing/delivered pregnancy rates per retrieval (26% versus 44%). Day 3 embryos with > or = 8 blastomeres resulted in a significantly higher pregnancy rate (53%) than day 3 embryos with < 8 cells (23%) and day 2 embryos with > or = 4 cells (31%) or < 4 cells (11%). CONCLUSION(S):Day 3 ET was associated with a significant increase in implantation and pregnancy rates. Delaying the ET until day 3 may permit the selection of more viable embryos than on day 2. The absence of glucose and phosphate from the culture media is compatible with good IVF outcomes.

journal_name

Fertil Steril

journal_title

Fertility and sterility

authors

Carrillo AJ,Lane B,Pridman DD,Risch PP,Pool TB,Silverman IH,Cook CL

doi

10.1016/s0015-0282(97)00499-8

subject

Has Abstract

pub_date

1998-02-01 00:00:00

pages

329-34

issue

2

eissn

0015-0282

issn

1556-5653

pii

S0015028297004998

journal_volume

69

pub_type

杂志文章

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