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Human serum and follicular fluid stimulate hyperactivation of human spermatozoa after preincubation.

Abstract：

OBJECTIVE:To test the effects of steroid-containing media such as follicular fluid (FF) and serum and of steroid-free but human protein-rich medium on hyperactivation (HA) of human spermatozoa with and without a preincubation period. DESIGN:Analysis of HA using a computer-aided sperm analysis system. SETTING:Academic hospital and andrologic laboratory. PATIENTS:Twenty-six sperm samples were obtained from 26 different IVF patients. INTERVENTIONS:None. RESULTS:After a preincubation period of 3 hours, FF and serum enhanced the HA after 1 hour. A human plasma protein-rich solution did not. Without preincubation, no stimulation of HA was observed. CONCLUSION:Steroids may be involved in HA, and a preincubation period may be required for an appropriate FF-induced HA.

journal_name

Fertil Steril

journal_title

Fertility and sterility

authors

Kulin S,Bastiaans BA,Hollanders HM,Janssen HJ,Goverde HJ

doi

10.1016/s0015-0282(16)57191-x

subject

Has Abstract

pub_date

1994-12-01 00:00:00

pages

1234-7

issue

6

eissn

0015-0282

issn

1556-5653

pii

S0015-0282(16)57191-X

journal_volume

62

pub_type

杂志文章

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