当前位置： SCI文献检索 > FERTILITY AND STERILITY期刊下所有文献 > Long-term follow-up of cryopreserved hemi-ovary autografts in ewes: pregnancies, births, and histologic assessment.

Long-term follow-up of cryopreserved hemi-ovary autografts in ewes: pregnancies, births, and histologic assessment.

Abstract：

OBJECTIVE:To evaluate a 2-year follow-up of cryopreserved hemi-ovary autografts in ewes. DESIGN:Animal study. SERTTING: University fertility center, Hospices Civils de Lyon; Ecole Nationale Vétérinaire de Lyon, INSERM U 418 Hocaron;pital Debrousse, Lyon; and Hôpital Edouard Herriot, Lyon, France. PATIENT(S):Grivette ewes. INTERVENTION(S):Recently we reported four pregnancies and six live births after transplantation of frozen-thawed hemi-ovary in six different ewes. The four remaining ewes were monitored for 2 years. After the last birth, the autografted ovary was removed in each ewe during a final laparotomy. The entire grafted ovary was sliced to estimate the remaining primordial follicle population 2 years after grafting. MAIN OUTCOME MEASURE(S):Uterine ultrasound scanning was performed to diagnose pregnancy. Histological assessment of the grafted ovary was performed after delivery. RESULT(S):The four remaining ewes began new gestations. For two of them, this was a second gestation obtained more than 2 years after the autograft. These two ewes delivered male lambs, which died immediately after delivery because of distocia. The lambs were both oversized for gestational age; autopsy found no malformation. A twin pregnancy of a healthy male and a healthy female occurred in May 2002, and a singleton male was born in February 2002. All grafted ovaries showed drastic reduction in follicle population. CONCLUSION(S):Frozen-thawed ovary autograft allowed recovery of fertility a very long time after the procedure despite a drastic reduction in the total number of follicles.

journal_name

Fertil Steril

journal_title

Fertility and sterility

authors

Salle B,Demirci B,Franck M,Berthollet C,Lornage J

doi

10.1016/s0015-0282(03)00554-5

keywords:

subject

Has Abstract

pub_date

2003-07-01 00:00:00

pages

172-7

issue

1

eissn

0015-0282

issn

1556-5653

pii

S0015028203005545

journal_volume

80

pub_type

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