当前位置： SCI文献检索 > FERTILITY AND STERILITY期刊下所有文献 > Supplemented tissue culture medium 199 is a better medium for in vitro maturation of oocytes from women with polycystic ovary syndrome women than human tubal fluid.

Supplemented tissue culture medium 199 is a better medium for in vitro maturation of oocytes from women with polycystic ovary syndrome women than human tubal fluid.

Abstract：

OBJECTIVE:To compare oocyte maturation, fertilization and cleavage rates, and embryonic developmental quality after culture of human immature oocytes from polycystic ovary syndrome (PCOS) patients in human tubal fluid (HTF) or tissue culture medium (TCM) 199. DESIGN:Prospective, randomized, controlled trial. SETTING:University hospital. PATIENT(S):Thirteen women undergoing 23 in vitro maturation cycles, from whom 119 oocytes were retrieved. INTERVENTION(S):Cumulus-enclosed germinal vesicle-stage oocytes matured in TCM-199-supplemented or HTF-supplemented media. MAIN OUTCOME MEASURE(S):Oocyte maturation and fertilization rates, embryonic developmental quality. RESULT(S):Significant differences were observed between TCM 199 and HTF regarding maturation rate (82% vs. 56.9%), fertilization rate (70% vs. 39.4%), and embryo quality (81.3% vs. 41.7%). CONCLUSION(S):Human tubal fluid medium, although widely used for embryo fertilization and maintenance in IVF techniques, is not an appropriate medium for the maturation of oocytes obtained from PCOS patients in nonstimulated cycles.

journal_name

Fertil Steril

journal_title

Fertility and sterility

authors

de Araujo CH,Nogueira D,de Araujo MC,Martins Wde P,Ferriani RA,dos Reis RM

doi

10.1016/j.fertnstert.2007.11.082

subject

Has Abstract

pub_date

2009-02-01 00:00:00

pages

509-13

issue

2

eissn

0015-0282

issn

1556-5653

pii

S0015-0282(07)04147-7

journal_volume

91

pub_type

杂志文章,随机对照试验

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