Abstract：
:The authors compared the three most commonly used sperm preparation techniques--swim-up, fall-down, and Percoll gradient--for their ability to recover highly motile sperm and minimize bacterial contamination. Eleven human semen samples collected by masturbation were used and run in parallel with the three methods. A semiquantitative bacterial analysis was performed in all samples and results expressed in colony-forming units per milliliter (CFU/ml). The Percoll gradient technique resulted in an average sperm concentration of 5.81 +/- 4.4 X 10(6) ml, and the average bacterial concentration dropped from 8.66 +/- 12.96 X 10(3) CFU/ml in semen to 0.01 +/- 0.03 X 10(3) CFU/ml. The bacterial count was not significantly different when the raw semen was compared with the swim-up or the fall-down preparations. The authors conclude that the Percoll gradient method yields an adequate sperm concentration, with high motility and improved morphology, while eliminating bacterial contamination.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Sun LS,Gastaldi C,Peterson EM,de la Maza LM,Stone SCdoi
10.1016/s0015-0282(16)59481-3subject
Has Abstractpub_date
1987-10-01 00:00:00pages
659-63issue
4eissn
0015-0282issn
1556-5653pii
S0015-0282(16)59481-3journal_volume
48pub_type
杂志文章abstract：OBJECTIVE:To extend and confirm earlier observations that serial CA-125 determinations appear to have prognostic value in some infertile women with surgically treated endometriosis using a new automated enzyme immunoassay. DESIGN:A prospective consecutive case series. SETTING:A university-based tertiary practice. PA...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1995-08-01 00:00:00
abstract：OBJECTIVE:To determine the impact of a cryopreservation program on pregnancy rates and multiple-pregnancy rates in ART cycles. DESIGN:Retrospective study. SETTING:University teaching hospital. PATIENT(S):Women who underwent stimulation for in vitro fertilization at the Jones Institute for Reproductive Medicine betwe...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(00)01661-7
更新日期：2001-01-01 00:00:00
abstract：OBJECTIVE:To examine the emotional, sexual, physical, and quality-of-life (QOL) impact on infertile women awaiting oocyte donation. DESIGN:Cross-sectional cohort study. SETTING:Cancer center and reproductive medicine center. PATIENT(S):Fifty infertile women awaiting oocyte donation, enrolled from October 2006 to Feb...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2010.10.004
更新日期：2011-02-01 00:00:00
abstract：OBJECTIVE:To study the expression and function of adiponectin and its receptors in mouse and human follicle cells and in early embryo development. DESIGN:Whole ovaries, granulosa cells, and cumulus-oocyte complexes isolated from immature mice before and during hormone-induced ovulation were used to analyze the express...
journal_title：Fertility and sterility
pub_type： 杂志文章,多中心研究
doi：10.1016/j.fertnstert.2012.04.050
更新日期：2012-08-01 00:00:00
abstract：OBJECTIVE:To assess the efficiency of two vitrification protocols to cryopreserve human preantral follicles with the use of a xenografting model. DESIGN:Pilot study. SETTING:Gynecology research unit in a university hospital. PATIENT(S):Ovarian biopsies were obtained from seven women aged 30-41 years. INTERVENTION(S...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2012.07.1109
更新日期：2012-11-01 00:00:00
abstract：OBJECTIVE:To describe an ovarian abscess presenting very late after oocyte retrieval for IVF with several unusual clinical features. DESIGN:Case report. SETTING:Academic medical center. PATIENT(S):A 35-year-old nulliparous woman underwent IVF with uncomplicated transvaginal oocyte retrieval (TVOR), resulting in a di...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2005.12.045
更新日期：2006-07-01 00:00:00
abstract：OBJECTIVE:To evaluate the efficacy of the second micro-testicular sperm extraction (TESE)in men with nonobstructive azoospermia in whom the first micro-TESE failed. DESIGN:Retrospective. SETTING:Private clinic. PATIENT(S):One hundred twenty-five men with nonobstructive azoospermia with failed previous micro-TESE. Th...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2020.10.005
更新日期：2020-12-23 00:00:00
abstract：:This article has been withdrawn at the request of the author(s) and/or editor. The Publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at http://www.elsevier.com/locate/withdrawalpolicy. ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2013.08.053
更新日期：2013-10-07 00:00:00
abstract：OBJECTIVE:To establish an intracytoplasmic sperm injection treatment program for couples with male infertility and to determine those factors important for success. DESIGN:A retrospective analysis of 171 consecutive cycles of intracytoplasmic sperm injection concerning 145 infertile couples. SETTING:Infertility clini...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)57489-5
更新日期：1995-04-01 00:00:00
abstract：OBJECTIVE:To examine differences in specific protein expression from the surface of the human endometrium with respect to eventual pregnancy in infertile women. DESIGN:Laboratory study. SETTING:University hospital. PATIENT(S):Thirty-one women presenting for investigation into infertility at an assisted reproductive ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2007.01.094
更新日期：2008-09-01 00:00:00
abstract：:Forty years ago this month the first baby was born as a result of in vitro fertilization (IVF). The arrival of Louise Brown brought with her a new hope and possibility for the many men and women across the global struggling with infertility who previously had very few options for treatment or family building. In this ...
journal_title：Fertility and sterility
pub_type： 历史文章
doi：10.1016/j.fertnstert.2018.05.017
更新日期：2018-07-01 00:00:00
abstract：OBJECTIVE:To determine whether magnetic resonance imaging (MRI) and serum levels of CA-125 can be used to evaluate patients with endometriomas before initiating medical treatment. DESIGN:Comparative study before and after medical treatment. SETTING:Shimane Medical University Hospital, Izumo, Japan. PATIENTS:Eleven c...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章
doi：10.1016/s0015-0282(16)58087-x
更新日期：1996-02-01 00:00:00
abstract：:A total of 82 patients underwent 106 cycles in which immature oocytes were recovered after hCG priming and transferred at blastocyst-stage. After transfer, the implantation rate was 26.8%, and the clinical pregnancy rate was 51.9%. We found that the oocytes retrieved after hCG priming in women with high risk of ovaria...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2006.11.027
更新日期：2007-06-01 00:00:00
abstract：OBJECTIVE:To report the use of cumulative live-birth rates (CLBRs) per ovarian stimulation cycle to measure the success of IVF is proving to be the most accurate method for advising couples who failed to conceive, although the accuracy yielded is relatively low, and cycle outcome is highly dependent on the number of em...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2011.05.008
更新日期：2011-07-01 00:00:00
abstract：OBJECTIVE:To evaluate the cryopreservation of immature human oocytes obtained from unstimulated ovarian tissue. DESIGN:Immature prophase I oocytes were obtained from unstimulated follicles and were either cryopreserved or cultured as controls. Cryopreservation was performed in a programmable freezing machine using one...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1994-06-01 00:00:00
abstract：OBJECTIVE:To examine the effect of supraphysiologic doses of testosterone (T) on plasma total homocysteine (tHcy) concentrations in patients with Klinefelter's syndrome (KS). DESIGN:Prospective clinical study. SETTING:University hospital. PATIENT(S):Thirty-two newly diagnosed male patients with KS, and 20 healthy, v...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2003.11.029
更新日期：2004-05-01 00:00:00
abstract：OBJECTIVE:To develop molecular tools increasing the sensitivity of breast cancer micrometastases detection within ovarian tissue cryopreserved for fertility preservation. DESIGN:Expression of breast markers was evaluated by quantitative polymerase chain reaction in ovarian tissue from patients with benign or cancerous...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2015.04.036
更新日期：2015-08-01 00:00:00
abstract：OBJECTIVE:To describe the use of a Malecot catheter as a stent after radical trachelectomy (RT). DESIGN:Case report. SETTING:Assisted conception unit at a teaching hospital in the United Kingdom. PATIENT(S):A 36-year-old woman undergoing IVF after her cervix had been excised for cervical carcinoma. Previous attempts...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2004.11.081
更新日期：2005-06-01 00:00:00
abstract：OBJECTIVE:To assess cycle outcomes when antimüllerian hormone (AMH) is ultralow (≤0.16 ng/mL) and to determine which parameters contribute to the probability of cycle cancellation and/or outcome. DESIGN:Retrospective analysis. SETTING:Not applicable. PATIENT(S):5,087 (7.3%) fresh and 243 (1.5%) thawed cycles with ul...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2015.10.004
更新日期：2016-02-01 00:00:00
abstract：:This report describes our initial experience with elective single blastocyst transfer in 19 patients who had a mean age of 36.3 +/- 2.4 years. The ongoing pregnancy rate, 53% after the fresh embryo transfer and 68% when thaw cycles are included, suggests that single blastocyst transfer has a place in this relatively o...
journal_title：Fertility and sterility
pub_type： 信件
doi：10.1016/j.fertnstert.2003.10.050
更新日期：2004-06-01 00:00:00
abstract：:The transcervical division of uterine septa is usually described as a simple procedure associated with minimal morbidity. Two cases are presented describing late hemorrhage after this procedure. Approaches to avoiding this complication as well as management principles are described. ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)54984-x
更新日期：1992-04-01 00:00:00
abstract：OBJECTIVE:To review the impacts of obesity on women's reproductive health and fertility. DESIGN:Literature review. SETTING:Academic medical center. PATIENT(S):Forty articles are referenced. The number of patients evaluated in each of the studies varies from 33 to 213,208. INTERVENTION(S):Articles were identified fr...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2008.02.141
更新日期：2009-05-01 00:00:00
abstract：:The chromosome analysis of undivided oocytes and polyploid embryos within a human in vitro fertilization and embryo replacement program offers a unique opportunity to develop correlations between a successful or unsuccessful fertilization and the maturity of the oocytes or the quality of the sperm. Furthermore, it pro...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)48394-9
更新日期：1985-02-01 00:00:00
abstract：:One hundred and two women with insulin-resistant polycystic ovary syndrome were randomized to treatment with a vitamin B preparation, metformin, or both, in conjunction with standard infertility treatment. Plasma homocysteine levels were significantly reduced by both B vitamins and metformin, but to a greater degree b...
journal_title：Fertility and sterility
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.fertnstert.2006.11.071
更新日期：2007-07-01 00:00:00
abstract：OBJECTIVE:To identify and treat the gamete responsible for complete fertilization failure with intracytoplasmic sperm injection (ICSI) using a newly proposed assisted gamete treatment (AGT). DESIGN:Prospective cohort study. SETTING:Center for reproductive medicine. PATIENT(S):One-hundred and fourteen couples with an...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2020.04.044
更新日期：2020-09-01 00:00:00
abstract：OBJECTIVE:To determine the prevalence of nucleolar channel systems (NCSs) by uterine region, applying continuous quantification. DESIGN:Prospective clinical study. SETTING:Tertiary care academic medical center. PATIENT(S):Forty-two naturally cycling women who underwent hysterectomy for benign indications. INTERVENT...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2012.10.027
更新日期：2013-02-01 00:00:00
abstract：OBJECTIVE:To characterize and to purify the growth substances for mouse embryo fibroblasts (NIH/3T3) and endometrial-like cells of the peritoneal fluid of women with endometriosis. DESIGN:A pool of 102 mL of peritoneal fluid (PF) collected from 24 women with laparoscopic evidence of endometriosis was dialyzed and ther...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1993-01-01 00:00:00
abstract：:Both EST and Mayer-Rokitansky-Kuster-Hauser syndrome are uncommon disorders. This report illustrates a case of these two rare conditions occurring in the same patient. Patients with Mayer-Rokitansky-Kuster-Hauser syndrome, although not appearing to be at increased risk for germ cell tumors, unlike testicular feminizat...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)54562-2
更新日期：1991-09-01 00:00:00
abstract：OBJECTIVE:To estimate the incidence of endometriosis in an operative cohort of women seeking clinical care and in a matched population cohort to delineate more fully the scope and magnitude of endometriosis in the context of and beyond clinical care. DESIGN:Matched-exposure cohort design. SETTING:Surgical centers in ...
journal_title：Fertility and sterility
pub_type： 杂志文章,多中心研究
doi：10.1016/j.fertnstert.2011.05.087
更新日期：2011-08-01 00:00:00
abstract：OBJECTIVE:To evaluate the level of variance produced in a multicenter study with the use of a computer-assisted sperm morphology analyzer. DESIGN:A multicenter, prospective, blinded study. SETTING:Assisted reproduction research laboratories. PATIENT(S):Semen samples produced for assisted reproductive procedures. IN...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章,多中心研究,随机对照试验
doi：10.1016/s0015-0282(98)00421-x
更新日期：1999-01-01 00:00:00
